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The map label for this gene is glpK-2 [H]

Identifier: 116329132

GI number: 116329132

Start: 3032545

End: 3034092

Strand: Direct

Name: glpK-2 [H]

Synonym: LBL_2555

Alternate gene names: 116329132

Gene position: 3032545-3034092 (Clockwise)

Preceding gene: 116329131

Following gene: 116329133

Centisome position: 83.9

GC content: 43.73

Gene sequence:

>1548_bases
ATGACTAATCCACAGGGGAAATACGTCCTTTCCATCGATAGCGGAGGAAGTGGAATTCGTGCATTTTTATTGGATAGAAA
AGGTAAAATCACCGAAAGACAATATGAAAAAACTCCTCCAAACATCCCCGAAGCGGGAGCCTTGGAACACGATCCGGAAA
TACTATGGAAGTCCCTACTTATTCTTTTGAAAAAAATTCAAAAGAATAAACAAATCACGAAAGAAATAGCTGCATTAGGA
ATTTGTAATCAACGAGGATCCTTTTTATTATGGGAAAGATTTTCCGGCAAACCCTTGACCCCTTTGATCAGTTGGGCGGA
TGTTCGTTCGCATAAAACCGCGGAGGCTATGAATCGGAATCCGATCTGGAAAACCATTCGTTTTGTTTCCACACTCGTCG
GAAGATTGACGAATCATCCAATGATGATCGCTACTTCTATGCTTAGGTTTACAACCGATCACGCAACTTGTAGACTGAAA
TTTGTTTTGGACGAAAATCCGGAGTTAAAACGCAGATGTAAAAAGGGTGAAGTCCTTTTCGGCACCTTAGATACCTGGTT
CATTTATAAACTCACAGGTGGGAAACAACATCTTACCGATTCGTCTAACGCGGTCGCAACAGGAATGTTTAATCCCTTCC
AATTAATTTGGAATCAACCGATCCTTACAATTTTTGGCATTCCCGCAAATCTGTTTCCGGAAGTGAAAGACAGCAACGGA
GATTTCGGTCATACCTTACCCGAGTTTTTAGGAGGACATTCGGTGCCGATTCGAGCATCAATCGGTGATCAGATGGCCGC
TCTTTTCGGTCAATGTTGTTTTGAGCCGGGTGAAGTGAAAATCTCCCAAGGCTCCGGAGCCTTCGTAGATATCAACATCG
GCCTCAAGGCACAATTATCCAAACGCGGACTTTTTCCTATGATCGCTTGGAGAATCGACGGAAAAACCACGTATATGTTG
GAAGGATATGTTGCAACCGCCGGAACTCTAATTGACTGGTTGGGTAAAGGGATCGGACTTTCCGACACCCCTAAAGTTCT
TAACGAACTTGCGGCGCAAGCCGAAGACACGGAAGGAGTCGTTTTTATTCCAACTCCTACAGGCGCTCGATTCCCTTACT
TTAATCCTCGCGCCAAGGCTTCCGTGATCGGCCTTTCACTTGCATCTCATAGAAGGCATGTAGCGAGAGCCGTCTTGGAA
GGAATTGCTCTTAGCCTCTACGAAATTTTGGAAGGAATTCAACAAGATACAAAAATCAAGATTAAGGATATCAAAGTGGA
TGGAGGAGTTTCTCAATCCGATATTCTACTCCAATGTCTTTCGGACTTTTCGAACGTGAGAGTGGATCGTGCTCCCGAAC
CGGATATGACCGCAACAGGTGCAGCGTATCTTGCGGGTTTATCCATCGGCTTTTGGAAAAATTTAGGAGAACTCAAAAGT
CTGCAAAAAGGTTATAAGTCTTTCGAACCGAAAATGGATTCAACCCAAAGAGCCCAAAAAATTCAACGCTGGAAGAAGGC
AGTTACAGCAACTCTTTTCATCGAATAA

Upstream 100 bases:

>100_bases
TTTTGAGACAAGCCCTAATCCTCAGAAAGAAATGGACAAAATCTTCATCTTTCGATTATTTTGATTTCTCTTTAAAAAAA
CTGAATACTCGGAGATTTGG

Downstream 100 bases:

>100_bases
GTTTCGAAAGATAAGACAAAAATGACGCCCTTTCCGGACTTGAAAAACGTGGGTTAGTATGCGAGATTTCACAACAAGGC
AAGAAAGCCAACAGAAGGAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTNPQGKYVLSIDSGGSGIRAFLLDRKGKITERQYEKTPPNIPEAGALEHDPEILWKSLLILLKKIQKNKQITKEIAALG
ICNQRGSFLLWERFSGKPLTPLISWADVRSHKTAEAMNRNPIWKTIRFVSTLVGRLTNHPMMIATSMLRFTTDHATCRLK
FVLDENPELKRRCKKGEVLFGTLDTWFIYKLTGGKQHLTDSSNAVATGMFNPFQLIWNQPILTIFGIPANLFPEVKDSNG
DFGHTLPEFLGGHSVPIRASIGDQMAALFGQCCFEPGEVKISQGSGAFVDINIGLKAQLSKRGLFPMIAWRIDGKTTYML
EGYVATAGTLIDWLGKGIGLSDTPKVLNELAAQAEDTEGVVFIPTPTGARFPYFNPRAKASVIGLSLASHRRHVARAVLE
GIALSLYEILEGIQQDTKIKIKDIKVDGGVSQSDILLQCLSDFSNVRVDRAPEPDMTATGAAYLAGLSIGFWKNLGELKS
LQKGYKSFEPKMDSTQRAQKIQRWKKAVTATLFIE

Sequences:

>Translated_515_residues
MTNPQGKYVLSIDSGGSGIRAFLLDRKGKITERQYEKTPPNIPEAGALEHDPEILWKSLLILLKKIQKNKQITKEIAALG
ICNQRGSFLLWERFSGKPLTPLISWADVRSHKTAEAMNRNPIWKTIRFVSTLVGRLTNHPMMIATSMLRFTTDHATCRLK
FVLDENPELKRRCKKGEVLFGTLDTWFIYKLTGGKQHLTDSSNAVATGMFNPFQLIWNQPILTIFGIPANLFPEVKDSNG
DFGHTLPEFLGGHSVPIRASIGDQMAALFGQCCFEPGEVKISQGSGAFVDINIGLKAQLSKRGLFPMIAWRIDGKTTYML
EGYVATAGTLIDWLGKGIGLSDTPKVLNELAAQAEDTEGVVFIPTPTGARFPYFNPRAKASVIGLSLASHRRHVARAVLE
GIALSLYEILEGIQQDTKIKIKDIKVDGGVSQSDILLQCLSDFSNVRVDRAPEPDMTATGAAYLAGLSIGFWKNLGELKS
LQKGYKSFEPKMDSTQRAQKIQRWKKAVTATLFIE
>Mature_514_residues
TNPQGKYVLSIDSGGSGIRAFLLDRKGKITERQYEKTPPNIPEAGALEHDPEILWKSLLILLKKIQKNKQITKEIAALGI
CNQRGSFLLWERFSGKPLTPLISWADVRSHKTAEAMNRNPIWKTIRFVSTLVGRLTNHPMMIATSMLRFTTDHATCRLKF
VLDENPELKRRCKKGEVLFGTLDTWFIYKLTGGKQHLTDSSNAVATGMFNPFQLIWNQPILTIFGIPANLFPEVKDSNGD
FGHTLPEFLGGHSVPIRASIGDQMAALFGQCCFEPGEVKISQGSGAFVDINIGLKAQLSKRGLFPMIAWRIDGKTTYMLE
GYVATAGTLIDWLGKGIGLSDTPKVLNELAAQAEDTEGVVFIPTPTGARFPYFNPRAKASVIGLSLASHRRHVARAVLEG
IALSLYEILEGIQQDTKIKIKDIKVDGGVSQSDILLQCLSDFSNVRVDRAPEPDMTATGAAYLAGLSIGFWKNLGELKSL
QKGYKSFEPKMDSTQRAQKIQRWKKAVTATLFIE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI88196792, Length=506, Percent_Identity=33.201581027668, Blast_Score=339, Evalue=4e-93,
Organism=Homo sapiens, GI4504007, Length=518, Percent_Identity=31.2741312741313, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI189409120, Length=518, Percent_Identity=31.2741312741313, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI41393575, Length=523, Percent_Identity=29.8279158699809, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI42794763, Length=524, Percent_Identity=31.1068702290076, Blast_Score=246, Evalue=3e-65,
Organism=Escherichia coli, GI1790361, Length=513, Percent_Identity=35.4775828460039, Blast_Score=325, Evalue=6e-90,
Organism=Escherichia coli, GI1789987, Length=507, Percent_Identity=26.0355029585799, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790005, Length=527, Percent_Identity=22.9601518026565, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71986140, Length=523, Percent_Identity=29.4455066921606, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17535599, Length=511, Percent_Identity=28.7671232876712, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI71986147, Length=297, Percent_Identity=31.986531986532, Blast_Score=155, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6321755, Length=509, Percent_Identity=28.2907662082515, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI21357867, Length=520, Percent_Identity=30.9615384615385, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24656367, Length=520, Percent_Identity=30.3846153846154, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24656372, Length=391, Percent_Identity=32.4808184143223, Blast_Score=235, Evalue=4e-62,
Organism=Drosophila melanogaster, GI17864214, Length=518, Percent_Identity=29.7297297297297, Blast_Score=234, Evalue=9e-62,
Organism=Drosophila melanogaster, GI24654573, Length=444, Percent_Identity=31.0810810810811, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24655555, Length=481, Percent_Identity=26.8191268191268, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24655560, Length=481, Percent_Identity=26.8191268191268, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24655570, Length=481, Percent_Identity=26.8191268191268, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24655574, Length=481, Percent_Identity=26.8191268191268, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24655565, Length=481, Percent_Identity=26.8191268191268, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI19922058, Length=511, Percent_Identity=25.6360078277886, Blast_Score=178, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24652871, Length=505, Percent_Identity=25.5445544554455, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45552909, Length=342, Percent_Identity=30.7017543859649, Blast_Score=171, Evalue=9e-43,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 57011; Mature: 56880

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPQGKYVLSIDSGGSGIRAFLLDRKGKITERQYEKTPPNIPEAGALEHDPEILWKSLL
CCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
ILLKKIQKNKQITKEIAALGICNQRGSFLLWERFSGKPLTPLISWADVRSHKTAEAMNRN
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHCCC
PIWKTIRFVSTLVGRLTNHPMMIATSMLRFTTDHATCRLKFVLDENPELKRRCKKGEVLF
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHCCCCCEEE
GTLDTWFIYKLTGGKQHLTDSSNAVATGMFNPFQLIWNQPILTIFGIPANLFPEVKDSNG
EECCEEEEEEEECCCHHCCCCCCCEEECCCCHHHHHHCCCEEEEECCCHHHCCCCCCCCC
DFGHTLPEFLGGHSVPIRASIGDQMAALFGQCCFEPGEVKISQGSGAFVDINIGLKAQLS
CCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCEEEHH
KRGLFPMIAWRIDGKTTYMLEGYVATAGTLIDWLGKGIGLSDTPKVLNELAAQAEDTEGV
CCCCCEEEEEEECCCEEEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCE
VFIPTPTGARFPYFNPRAKASVIGLSLASHRRHVARAVLEGIALSLYEILEGIQQDTKIK
EEEECCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IKDIKVDGGVSQSDILLQCLSDFSNVRVDRAPEPDMTATGAAYLAGLSIGFWKNLGELKS
EEEEEECCCCCHHHHHHHHHHHHHHCEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHH
LQKGYKSFEPKMDSTQRAQKIQRWKKAVTATLFIE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEC
>Mature Secondary Structure 
TNPQGKYVLSIDSGGSGIRAFLLDRKGKITERQYEKTPPNIPEAGALEHDPEILWKSLL
CCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
ILLKKIQKNKQITKEIAALGICNQRGSFLLWERFSGKPLTPLISWADVRSHKTAEAMNRN
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHCCC
PIWKTIRFVSTLVGRLTNHPMMIATSMLRFTTDHATCRLKFVLDENPELKRRCKKGEVLF
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHCCCCCEEE
GTLDTWFIYKLTGGKQHLTDSSNAVATGMFNPFQLIWNQPILTIFGIPANLFPEVKDSNG
EECCEEEEEEEECCCHHCCCCCCCEEECCCCHHHHHHCCCEEEEECCCHHHCCCCCCCCC
DFGHTLPEFLGGHSVPIRASIGDQMAALFGQCCFEPGEVKISQGSGAFVDINIGLKAQLS
CCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCEEEHH
KRGLFPMIAWRIDGKTTYMLEGYVATAGTLIDWLGKGIGLSDTPKVLNELAAQAEDTEGV
CCCCCEEEEEEECCCEEEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCE
VFIPTPTGARFPYFNPRAKASVIGLSLASHRRHVARAVLEGIALSLYEILEGIQQDTKIK
EEEECCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IKDIKVDGGVSQSDILLQCLSDFSNVRVDRAPEPDMTATGAAYLAGLSIGFWKNLGELKS
EEEEEECCCCCHHHHHHHHHHHHHHCEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHH
LQKGYKSFEPKMDSTQRAQKIQRWKKAVTATLFIE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA