Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 116329126

GI number: 116329126

Start: 3022731

End: 3024011

Strand: Direct

Name: purD [H]

Synonym: LBL_2549

Alternate gene names: 116329126

Gene position: 3022731-3024011 (Clockwise)

Preceding gene: 116329125

Following gene: 116329127

Centisome position: 83.63

GC content: 44.11

Gene sequence:

>1281_bases
TTGCAAGTTAAGTTAAAAGTCCTTCTCATCGGTTCAGGAGGCAGAGAAAGTGCAATCGCGTTCCATCTTCGTAAGTCCTC
ATTGTTAAGCGAATTAAAAGTTTTTCCCGGAAACGGCGGTTTTCCGGACAACGAGATTCTTCCCTCCGATTCGTTTAATA
TTTTGTCCAAAGAATCGGTTCAGTCTTTTTTAAAACTGAATCCTTTCGATTTTATCGTGGTCGGACCGGAAGACCCTCTA
GTTGCAGGCTTTGCGGATTGGGCGGCCGAATTGGAGATTCCCACTTTTGGTCCCGATTCCTACTGTGCACAAGTCGAAGG
TTCCAAAAATTTCGCGAAATCTTTAATGGTCGAAGCGAACATTCCTACGGCGGTGTATAAAACGTTTACGGAATATTCCG
CTTCCTTAAATTATCTAGAATCCCAAACGGTTCCCATCGTAATCAAAGCAGACGGGCTGGCCGCCGGAAAAGGCGTCACA
GTAGCCACGACAAAAAAAATGGCGATGAACGCACTCAAAGAAATCTTTAAAGATAAAAAATTCGGAGAAAGCGGCAATCA
AGTTGTCATTGAAGAGTTCATGGAGGGCCAAGAAGCGTCCATTTTCGCCGTTTCCGACGGGGATTCCTACTTTCTTTTAC
CGGCAGCACAAGATCATAAAAGAGCGTTCGACGGAGACCAAGGTCCGAATACCGGGGGTATGGGAGCCTATTGTCCCGCT
CCCGTCATTACGGAAACTATATTACAAAAAGTGAAAGAAAAAATTTTCGATCGGATGTTTGAAACGTTCCGCAAGAAAGG
GCATCCATATCGCGGTCTTCTTTACGCCGGTTTGATGATTTCCCCGGACGGAGAACCGAAAGTCGTTGAATTCAACTGCA
GATTCGGAGATCCTGAAACCCAATGTGTACTTGCAATGTTCGACGGAGATTTATTAGAACTTCTTTATATAGCTTCTACA
GGCAAAATTAAAGAAGTACGAACATCGATAAAAAGCGGAGCCGCAACAGTAGTGGTCCTCGCTGCTCAGGGCTATCCGGA
TTCTTATGAAAAAAATATTCCGTTGAATCTTCCGGAAACTTCCGGTCAAAACGTTTATCTTTTTCATGCGGGGACATCAA
AAAAAGATGGAAAAGTTTTTTCATCAGGGGGGAGAATCCTCGGAGTAGTCGCTCAAGGGACCGATCTTAGGAATTCGGTC
GACCAAGCTTATTCTTTCCTGGAAAAAATCCAGGTTCCAAAAACTTTTTATAGAAAAGACATCGGACACAAGGCCCTTTA
A

Upstream 100 bases:

>100_bases
TTTCTTTCCAAAGCCGCTCCCGATGTAGTGGAGAAAGAAAGAGAAAAACTCAAAATCTTCACCGATAAAGTGGAGGTTCT
GAAAAAGGGGATTCAAAATC

Downstream 100 bases:

>100_bases
CCTATGCCATTTTTGATTTCTGGAAATCTGGATCTTGTGGAGAGACATAGCCTCAAGAAAATGGAAAAATTTCTCGGGAT
CATCCTATCTCCGGTTCAAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MQVKLKVLLIGSGGRESAIAFHLRKSSLLSELKVFPGNGGFPDNEILPSDSFNILSKESVQSFLKLNPFDFIVVGPEDPL
VAGFADWAAELEIPTFGPDSYCAQVEGSKNFAKSLMVEANIPTAVYKTFTEYSASLNYLESQTVPIVIKADGLAAGKGVT
VATTKKMAMNALKEIFKDKKFGESGNQVVIEEFMEGQEASIFAVSDGDSYFLLPAAQDHKRAFDGDQGPNTGGMGAYCPA
PVITETILQKVKEKIFDRMFETFRKKGHPYRGLLYAGLMISPDGEPKVVEFNCRFGDPETQCVLAMFDGDLLELLYIAST
GKIKEVRTSIKSGAATVVVLAAQGYPDSYEKNIPLNLPETSGQNVYLFHAGTSKKDGKVFSSGGRILGVVAQGTDLRNSV
DQAYSFLEKIQVPKTFYRKDIGHKAL

Sequences:

>Translated_426_residues
MQVKLKVLLIGSGGRESAIAFHLRKSSLLSELKVFPGNGGFPDNEILPSDSFNILSKESVQSFLKLNPFDFIVVGPEDPL
VAGFADWAAELEIPTFGPDSYCAQVEGSKNFAKSLMVEANIPTAVYKTFTEYSASLNYLESQTVPIVIKADGLAAGKGVT
VATTKKMAMNALKEIFKDKKFGESGNQVVIEEFMEGQEASIFAVSDGDSYFLLPAAQDHKRAFDGDQGPNTGGMGAYCPA
PVITETILQKVKEKIFDRMFETFRKKGHPYRGLLYAGLMISPDGEPKVVEFNCRFGDPETQCVLAMFDGDLLELLYIAST
GKIKEVRTSIKSGAATVVVLAAQGYPDSYEKNIPLNLPETSGQNVYLFHAGTSKKDGKVFSSGGRILGVVAQGTDLRNSV
DQAYSFLEKIQVPKTFYRKDIGHKAL
>Mature_426_residues
MQVKLKVLLIGSGGRESAIAFHLRKSSLLSELKVFPGNGGFPDNEILPSDSFNILSKESVQSFLKLNPFDFIVVGPEDPL
VAGFADWAAELEIPTFGPDSYCAQVEGSKNFAKSLMVEANIPTAVYKTFTEYSASLNYLESQTVPIVIKADGLAAGKGVT
VATTKKMAMNALKEIFKDKKFGESGNQVVIEEFMEGQEASIFAVSDGDSYFLLPAAQDHKRAFDGDQGPNTGGMGAYCPA
PVITETILQKVKEKIFDRMFETFRKKGHPYRGLLYAGLMISPDGEPKVVEFNCRFGDPETQCVLAMFDGDLLELLYIAST
GKIKEVRTSIKSGAATVVVLAAQGYPDSYEKNIPLNLPETSGQNVYLFHAGTSKKDGKVFSSGGRILGVVAQGTDLRNSV
DQAYSFLEKIQVPKTFYRKDIGHKAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=41.2735849056604, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=41.2735849056604, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=41.2735849056604, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=41.2735849056604, Blast_Score=346, Evalue=3e-95,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=42.4882629107981, Blast_Score=334, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=36.2352941176471, Blast_Score=250, Evalue=9e-67,
Organism=Saccharomyces cerevisiae, GI6321203, Length=441, Percent_Identity=39.2290249433107, Blast_Score=308, Evalue=1e-84,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=39.3939393939394, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=39.3939393939394, Blast_Score=290, Evalue=9e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46374; Mature: 46374

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVKLKVLLIGSGGRESAIAFHLRKSSLLSELKVFPGNGGFPDNEILPSDSFNILSKESV
CEEEEEEEEEECCCCCCEEEEEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHH
QSFLKLNPFDFIVVGPEDPLVAGFADWAAELEIPTFGPDSYCAQVEGSKNFAKSLMVEAN
HHHHHCCCCEEEEECCCCCEEEEHHHHHHEEECCCCCCCCCEEEECCCHHHHHHEEEECC
IPTAVYKTFTEYSASLNYLESQTVPIVIKADGLAAGKGVTVATTKKMAMNALKEIFKDKK
CCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCC
FGESGNQVVIEEFMEGQEASIFAVSDGDSYFLLPAAQDHKRAFDGDQGPNTGGMGAYCPA
CCCCCCEEHHHHHHCCCCCEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCC
PVITETILQKVKEKIFDRMFETFRKKGHPYRGLLYAGLMISPDGEPKVVEFNCRFGDPET
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCC
QCVLAMFDGDLLELLYIASTGKIKEVRTSIKSGAATVVVLAAQGYPDSYEKNIPLNLPET
EEEEEEECCCCEEHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCC
SGQNVYLFHAGTSKKDGKVFSSGGRILGVVAQGTDLRNSVDQAYSFLEKIQVPKTFYRKD
CCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHC
IGHKAL
CCCCCC
>Mature Secondary Structure
MQVKLKVLLIGSGGRESAIAFHLRKSSLLSELKVFPGNGGFPDNEILPSDSFNILSKESV
CEEEEEEEEEECCCCCCEEEEEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHH
QSFLKLNPFDFIVVGPEDPLVAGFADWAAELEIPTFGPDSYCAQVEGSKNFAKSLMVEAN
HHHHHCCCCEEEEECCCCCEEEEHHHHHHEEECCCCCCCCCEEEECCCHHHHHHEEEECC
IPTAVYKTFTEYSASLNYLESQTVPIVIKADGLAAGKGVTVATTKKMAMNALKEIFKDKK
CCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCC
FGESGNQVVIEEFMEGQEASIFAVSDGDSYFLLPAAQDHKRAFDGDQGPNTGGMGAYCPA
CCCCCCEEHHHHHHCCCCCEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCC
PVITETILQKVKEKIFDRMFETFRKKGHPYRGLLYAGLMISPDGEPKVVEFNCRFGDPET
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCC
QCVLAMFDGDLLELLYIASTGKIKEVRTSIKSGAATVVVLAAQGYPDSYEKNIPLNLPET
EEEEEEECCCCEEHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCC
SGQNVYLFHAGTSKKDGKVFSSGGRILGVVAQGTDLRNSVDQAYSFLEKIQVPKTFYRKD
CCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHC
IGHKAL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA