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The map label for this gene is yugF [H]

Identifier: 116329052

GI number: 116329052

Start: 2910861

End: 2911724

Strand: Direct

Name: yugF [H]

Synonym: LBL_2459

Alternate gene names: 116329052

Gene position: 2910861-2911724 (Clockwise)

Preceding gene: 116329051

Following gene: 116329053

Centisome position: 80.53

GC content: 41.09

Gene sequence:

>864_bases
ATGAAATTGATGTACGAATTATTCCTCGACTATTATCATTGGAAAAAGAAGAAATACATGAAAGAAACTCTCGGGTTTCG
GGACTTCAAAGTGGAAACCGGAGGAAATTTGATTTATTTTTTGGAAAAAAACCAGAAGCAAAATAAGACGATTCTTTTGA
TCCACGGTCTTCTGGATTCGGCAACGGGGCTTAGAAAAGTGGCTCCTAAAATTCGGCAGGACTATCGAATATTGATTCCG
GATATACCCGGATTCGGGAAAAGTAAACTCCCGCTGTTAAAGTATCTGTATCAAATAGACGTATTTGGAAATTTGATCTA
CGAGGCGATCCGAAAACTGAAGCTCACCAAACTGGTATTAGGCGGCCATTCCATGGGAGCTTTGATTGCAATGCACGTCG
CTCTCCGAGACCGCGAAAAAAGAATTTCTAAATTAGTTCTCATCTCTCCCGGAGGAATTCCGCATCCCCAACGGGACGAG
ATGAAAGAACTTCTATTCCCCAAAAACGAAAACGATCTTTTAAAATTGATTGAGGCTTTGTATTACGAAACTCCGGAATT
GCCGGGAAAAATCGCCAGAAAGGCTCTCATTCGATCTTGGAACGAACTTCCGAATCAATTTCTTACCGTAAACACTTTGG
AAAGAGAGGATGAAATTTTTCTCGGGAAAAGACTGAGAGAAATCGAAATTCCGGCGTTGATCGTGTCGGGCAAAGAAGAT
CCGATAACGGACGTCACAATGACAAAGAAACTGCATTCCTATCTGAAAAAAAGCAAGCTTGTTCTATTTCCCGAAGCGAA
ACATGCAATTCACATGGAAAAACCGGAAGAACTTTCCTTGGAAATCAACCGTTATCTCAATTGA

Upstream 100 bases:

>100_bases
GATTCAAACAAACCGTTTTCAGCTATGACTTTTTCTGATTTGTCGAGCTAAGAAGAGTTCCGAAAAGAGAAGGCAAAAAT
GAAATAGGGAATCTGCATTT

Downstream 100 bases:

>100_bases
AATCCGTCATATTGAAACAGTCTTTTCGAAAAATGAACATGACCCGTAAATTCAAATTGATCTCCCTCTTTTTTGTGGTT
TGGTTTATTGTAACGTGCAG

Product: Alpha/beta hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKLMYELFLDYYHWKKKKYMKETLGFRDFKVETGGNLIYFLEKNQKQNKTILLIHGLLDSATGLRKVAPKIRQDYRILIP
DIPGFGKSKLPLLKYLYQIDVFGNLIYEAIRKLKLTKLVLGGHSMGALIAMHVALRDREKRISKLVLISPGGIPHPQRDE
MKELLFPKNENDLLKLIEALYYETPELPGKIARKALIRSWNELPNQFLTVNTLEREDEIFLGKRLREIEIPALIVSGKED
PITDVTMTKKLHSYLKKSKLVLFPEAKHAIHMEKPEELSLEINRYLN

Sequences:

>Translated_287_residues
MKLMYELFLDYYHWKKKKYMKETLGFRDFKVETGGNLIYFLEKNQKQNKTILLIHGLLDSATGLRKVAPKIRQDYRILIP
DIPGFGKSKLPLLKYLYQIDVFGNLIYEAIRKLKLTKLVLGGHSMGALIAMHVALRDREKRISKLVLISPGGIPHPQRDE
MKELLFPKNENDLLKLIEALYYETPELPGKIARKALIRSWNELPNQFLTVNTLEREDEIFLGKRLREIEIPALIVSGKED
PITDVTMTKKLHSYLKKSKLVLFPEAKHAIHMEKPEELSLEINRYLN
>Mature_287_residues
MKLMYELFLDYYHWKKKKYMKETLGFRDFKVETGGNLIYFLEKNQKQNKTILLIHGLLDSATGLRKVAPKIRQDYRILIP
DIPGFGKSKLPLLKYLYQIDVFGNLIYEAIRKLKLTKLVLGGHSMGALIAMHVALRDREKRISKLVLISPGGIPHPQRDE
MKELLFPKNENDLLKLIEALYYETPELPGKIARKALIRSWNELPNQFLTVNTLEREDEIFLGKRLREIEIPALIVSGKED
PITDVTMTKKLHSYLKKSKLVLFPEAKHAIHMEKPEELSLEINRYLN

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=271, Percent_Identity=26.1992619926199, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786902, Length=250, Percent_Identity=24.4, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24649021, Length=264, Percent_Identity=25.3787878787879, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33453; Mature: 33453

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLMYELFLDYYHWKKKKYMKETLGFRDFKVETGGNLIYFLEKNQKQNKTILLIHGLLDS
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCEEEEEEECHHC
ATGLRKVAPKIRQDYRILIPDIPGFGKSKLPLLKYLYQIDVFGNLIYEAIRKLKLTKLVL
CCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
GGHSMGALIAMHVALRDREKRISKLVLISPGGIPHPQRDEMKELLFPKNENDLLKLIEAL
CCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHH
YYETPELPGKIARKALIRSWNELPNQFLTVNTLEREDEIFLGKRLREIEIPALIVSGKED
HHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCC
PITDVTMTKKLHSYLKKSKLVLFPEAKHAIHMEKPEELSLEINRYLN
CCCHHHHHHHHHHHHHHCCEEEEECCCCCEECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKLMYELFLDYYHWKKKKYMKETLGFRDFKVETGGNLIYFLEKNQKQNKTILLIHGLLDS
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCEEEEEEECHHC
ATGLRKVAPKIRQDYRILIPDIPGFGKSKLPLLKYLYQIDVFGNLIYEAIRKLKLTKLVL
CCHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
GGHSMGALIAMHVALRDREKRISKLVLISPGGIPHPQRDEMKELLFPKNENDLLKLIEAL
CCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHH
YYETPELPGKIARKALIRSWNELPNQFLTVNTLEREDEIFLGKRLREIEIPALIVSGKED
HHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCC
PITDVTMTKKLHSYLKKSKLVLFPEAKHAIHMEKPEELSLEINRYLN
CCCHHHHHHHHHHHHHHCCEEEEECCCCCEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]