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The map label for this gene is glpF [H]

Identifier: 116329034

GI number: 116329034

Start: 2889500

End: 2890222

Strand: Direct

Name: glpF [H]

Synonym: LBL_2439

Alternate gene names: 116329034

Gene position: 2889500-2890222 (Clockwise)

Preceding gene: 116329031

Following gene: 116329035

Centisome position: 79.94

GC content: 47.3

Gene sequence:

>723_bases
ATGTCATCGCCTGTTTTGGGAGAATTTTTAGGAACTTTCGTTCTCATACTTTTGGGTAACGGAGTTGTGGCCGGAGTTTT
ACTCGAAAGAAGCAAATCCAAAGATGGAGGTTGGATCGTAATCACCGCCGGTTGGGCTTGTGCGGTAATGATCGGAGTTT
TCACTTCGAACGCGTTTGGAAGTTCGGACGCCCATCTCAATCCCGCGGTAACACTCGCGTTTGCCGTCAAAACCGGAGAT
TATTCCAAATTAGCGTCGTATATTCCGGCACAAGTCGCGGGAGCTTTTTTCGGAGCGGTTTTCAATTATTTACACTACTT
TCCTCATTGGAAAGAAACAAAGGATCAAGGAAAAATCCTCGCGGCATTTTCCACGGAACCGGCAATTTCGCATATCGTGT
ATAATTTTTTCAGCGAATTCTTAGGCACCTTCTTACTCGTCCTCGGAATCGTTTCCATTTTCTCTCCTACGATGCGGGGT
TTAAACGTACATTTCGGAACGTTTCTCGTGGGAATTCTGGTTTGGAGCATCGGACTTTCCATGGGAGGAACCACGGGTTA
CGCAATCAATCCCGCAAGAGATCTCGGACCGAGGCTCGCTCATTTCATTTTGCCGATCTCCGGTAAAGGTCCCTCGAATT
GGAAATACGCGTGGTTGCCGATAATCGCTCCTCTGAGTGGCGGCGCTTGCGCCGGTTTTTTACTCGGATATTTGAAAGTT
TAA

Upstream 100 bases:

>100_bases
AAAAAAAGATGATAGAAAATGAAAAAATTCGAAGGTAGAAAAACGGATTGCCGATGTTAAACAACTGTGGATTTTAGTTC
CGATTTTCGTAGGGGGAAAA

Downstream 100 bases:

>100_bases
GAAAGGGCCGTTCCTTTTCTTGAATATGAATTTCATCAATGACATAAAAAAGAATTTTTCAAAATTCAATCCGGGCGTTC
CCGCATCGAAACTTTCAAGA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSSPVLGEFLGTFVLILLGNGVVAGVLLERSKSKDGGWIVITAGWACAVMIGVFTSNAFGSSDAHLNPAVTLAFAVKTGD
YSKLASYIPAQVAGAFFGAVFNYLHYFPHWKETKDQGKILAAFSTEPAISHIVYNFFSEFLGTFLLVLGIVSIFSPTMRG
LNVHFGTFLVGILVWSIGLSMGGTTGYAINPARDLGPRLAHFILPISGKGPSNWKYAWLPIIAPLSGGACAGFLLGYLKV

Sequences:

>Translated_240_residues
MSSPVLGEFLGTFVLILLGNGVVAGVLLERSKSKDGGWIVITAGWACAVMIGVFTSNAFGSSDAHLNPAVTLAFAVKTGD
YSKLASYIPAQVAGAFFGAVFNYLHYFPHWKETKDQGKILAAFSTEPAISHIVYNFFSEFLGTFLLVLGIVSIFSPTMRG
LNVHFGTFLVGILVWSIGLSMGGTTGYAINPARDLGPRLAHFILPISGKGPSNWKYAWLPIIAPLSGGACAGFLLGYLKV
>Mature_239_residues
SSPVLGEFLGTFVLILLGNGVVAGVLLERSKSKDGGWIVITAGWACAVMIGVFTSNAFGSSDAHLNPAVTLAFAVKTGDY
SKLASYIPAQVAGAFFGAVFNYLHYFPHWKETKDQGKILAAFSTEPAISHIVYNFFSEFLGTFLLVLGIVSIFSPTMRGL
NVHFGTFLVGILVWSIGLSMGGTTGYAINPARDLGPRLAHFILPISGKGPSNWKYAWLPIIAPLSGGACAGFLLGYLKV

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=236, Percent_Identity=36.0169491525424, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI22538420, Length=243, Percent_Identity=32.9218106995885, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=33.0645161290323, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI4502187, Length=251, Percent_Identity=31.0756972111554, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1790362, Length=250, Percent_Identity=32, Blast_Score=81, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71994009, Length=233, Percent_Identity=34.3347639484979, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17544068, Length=248, Percent_Identity=31.0483870967742, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531431, Length=245, Percent_Identity=33.469387755102, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531429, Length=245, Percent_Identity=33.469387755102, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32564052, Length=244, Percent_Identity=28.2786885245902, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17533613, Length=244, Percent_Identity=28.2786885245902, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71992966, Length=240, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=212, Percent_Identity=33.0188679245283, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322985, Length=273, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25514; Mature: 25383

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPVLGEFLGTFVLILLGNGVVAGVLLERSKSKDGGWIVITAGWACAVMIGVFTSNAFG
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCC
SSDAHLNPAVTLAFAVKTGDYSKLASYIPAQVAGAFFGAVFNYLHYFPHWKETKDQGKIL
CCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
AAFSTEPAISHIVYNFFSEFLGTFLLVLGIVSIFSPTMRGLNVHFGTFLVGILVWSIGLS
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MGGTTGYAINPARDLGPRLAHFILPISGKGPSNWKYAWLPIIAPLSGGACAGFLLGYLKV
CCCCCCEEECCHHHHHHHHHHHEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SSPVLGEFLGTFVLILLGNGVVAGVLLERSKSKDGGWIVITAGWACAVMIGVFTSNAFG
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCC
SSDAHLNPAVTLAFAVKTGDYSKLASYIPAQVAGAFFGAVFNYLHYFPHWKETKDQGKIL
CCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
AAFSTEPAISHIVYNFFSEFLGTFLLVLGIVSIFSPTMRGLNVHFGTFLVGILVWSIGLS
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MGGTTGYAINPARDLGPRLAHFILPISGKGPSNWKYAWLPIIAPLSGGACAGFLLGYLKV
CCCCCCEEECCHHHHHHHHHHHEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7565084; 11463916 [H]