Click here to switch to the map view.

The map label for this gene is rsbU-3 [H]

Identifier: 116329030

GI number: 116329030

Start: 2882936

End: 2884675

Strand: Direct

Name: rsbU-3 [H]

Synonym: LBL_2435

Alternate gene names: 116329030

Gene position: 2882936-2884675 (Clockwise)

Preceding gene: 116329029

Following gene: 116329031

Centisome position: 79.76

GC content: 40.29

Gene sequence:

>1740_bases
ATGAATTATTATCTCTTTTTTCCTATGGCGGCTTTTGTTGCAAACACGATGTTCATCGCATTTGTATATGCAAGACGTAC
CAACAACCCGATCATCCGCTCTTATTTACTCTATACGATCTCTTTAGATGCTTGGCTTTTCACTTACGCATTCGGTTGGT
CCTTTCCTCCGAAACCTTGGATGACTTGGGCATTTAAAATTCTCTCGATCACTTGGCTTCCTCTCGGTACGCTTTATTTG
GAATTTGTTTTTACGTTTCTAAACAGAAAACCCGGAATTTTTCTTTGGCTTTTTAGAATCGGAATTCCAATTTCGTATCT
AATCATCCTTTCCACGGACTGGGTCATCCAGGGAAGCATCCATTATTATTGGGGTAATGAAGGCGAACCCGGTCCTTTGT
ACTTTTTCGTAATTTTCAACTTCGTCGCGCTTCCCGCCCTTATTGGTTTGGGAGTTTTGACCCAGTCCTTTTTCGCTTCG
GGAAAAGATCAAAGAAAACAAACCGGATTGGCTATCCTAGGTTCCATGTTTGCGATATTCCTGAGTTTTTATTCAGAAAT
CATTCAGGTGGACGATCAGGGAAGAATGTTGTCCATGCCTTTGACTTCAATCGGAATCGTGATCCAATCTTTCTTGATTT
TTACCGCAATCACGAGATACGGATTTTTAAGAATCAACGTAGAGGGACTCGCAGCGGATTTGTTTCGGGACATTCACGAC
GGAATGATCTTGGTCAGGCGGGATCAATCCCTTTTTTTTATGAACGAATCCGCAATTAGAATATTAGGAATTTCGAATTC
CCCTCCTTCCCACTTTTACCCCTCCGATTTTTTAAAAGGATATCGGGAAAGTCCGGATCATTTTTCCAAAGAGTACGAAC
CCATTTTCAATGTCGATTGTAGAGGAGTCGAGCTCACAAGATCACACATAGAGTTAACGGGAAAAGAAAGCGGATACCTT
TACATTCTGAGAGATATCAGTGAAAAAATAGAATCCAGAGAAAAGATAGAACGTTTTTATTCCGATTTTAACAAAGATTT
GGAAATCGCAAAAATCGCACAAACTTCCGCGATTTCCACAATATTTCCGGAAAGTTCGAAATATAAGTTCTATTCCCACT
TTCAACCGTTCGAACTTGTAGGCGGAGATTTTTTACGAGCCTTAGAACGTTCCGACGGAAAATTAGATATCCTTTTTGCG
GATGTTTCGGGACACGGCATTTCCTCCGCTATGGTTGCCGGAATGTTATCTATCTCCTTCCAGTTGGTTATAGAAACAAA
TCCAACTCCGAAAAAAGCTCTGGAAAAAACCCAAACCTTACTTTTAAACGCGGTATTAAACCATCATGTTTCCGCAATTT
ATCTCTCGTTCGATCCAGATACAAAAACATTAGAATATTCTTATGCAGGTCATCATCCGATTTTGATTTTTAGGGAAGGG
GAAATCATTCCTCTGAGAGGCGTAGGAAGAATCTTGTTGATTACCCCGGACACGGAATTGAACAATTATACTTTTCAACT
CAAGAAGGGAGATATTTTATTCCTTTATTCGGATTGTCTTTTCGAAGTGAGAAGTCCCGAAGGAGAAATACTCGGATACG
AAAATTTCATGTTAAAAATGAACGAAATCTCGGTTTATACTCCCGTCAACATTCTCAAAACCTGCATAGGCCAGGCCCTG
GCCTTCGGCAACGGAAAACTCACAGACGACTTGACGATTTTGATTTTGGAGATTTTTTAA

Upstream 100 bases:

>100_bases
AATAGAAACCTAAAAAAGACTTAGAAATCTAAAATAGAAATTTTATAGTTTCAAATTCATTGTCGAAATCCATTCTCTAT
CCCAAGTTTCGTTCTTCCGT

Downstream 100 bases:

>100_bases
AGAATGAATCCGTACATTCTGATTCCTTTTTCGACCTTAATCATAAACGGATTTTTGTTCTCCTATGTAAGCGCACTTAA
AGGAAAATCCGAAACGGTTA

Product: Serine phosphatase RsbU, regulator of sigma subunit

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MNYYLFFPMAAFVANTMFIAFVYARRTNNPIIRSYLLYTISLDAWLFTYAFGWSFPPKPWMTWAFKILSITWLPLGTLYL
EFVFTFLNRKPGIFLWLFRIGIPISYLIILSTDWVIQGSIHYYWGNEGEPGPLYFFVIFNFVALPALIGLGVLTQSFFAS
GKDQRKQTGLAILGSMFAIFLSFYSEIIQVDDQGRMLSMPLTSIGIVIQSFLIFTAITRYGFLRINVEGLAADLFRDIHD
GMILVRRDQSLFFMNESAIRILGISNSPPSHFYPSDFLKGYRESPDHFSKEYEPIFNVDCRGVELTRSHIELTGKESGYL
YILRDISEKIESREKIERFYSDFNKDLEIAKIAQTSAISTIFPESSKYKFYSHFQPFELVGGDFLRALERSDGKLDILFA
DVSGHGISSAMVAGMLSISFQLVIETNPTPKKALEKTQTLLLNAVLNHHVSAIYLSFDPDTKTLEYSYAGHHPILIFREG
EIIPLRGVGRILLITPDTELNNYTFQLKKGDILFLYSDCLFEVRSPEGEILGYENFMLKMNEISVYTPVNILKTCIGQAL
AFGNGKLTDDLTILILEIF

Sequences:

>Translated_579_residues
MNYYLFFPMAAFVANTMFIAFVYARRTNNPIIRSYLLYTISLDAWLFTYAFGWSFPPKPWMTWAFKILSITWLPLGTLYL
EFVFTFLNRKPGIFLWLFRIGIPISYLIILSTDWVIQGSIHYYWGNEGEPGPLYFFVIFNFVALPALIGLGVLTQSFFAS
GKDQRKQTGLAILGSMFAIFLSFYSEIIQVDDQGRMLSMPLTSIGIVIQSFLIFTAITRYGFLRINVEGLAADLFRDIHD
GMILVRRDQSLFFMNESAIRILGISNSPPSHFYPSDFLKGYRESPDHFSKEYEPIFNVDCRGVELTRSHIELTGKESGYL
YILRDISEKIESREKIERFYSDFNKDLEIAKIAQTSAISTIFPESSKYKFYSHFQPFELVGGDFLRALERSDGKLDILFA
DVSGHGISSAMVAGMLSISFQLVIETNPTPKKALEKTQTLLLNAVLNHHVSAIYLSFDPDTKTLEYSYAGHHPILIFREG
EIIPLRGVGRILLITPDTELNNYTFQLKKGDILFLYSDCLFEVRSPEGEILGYENFMLKMNEISVYTPVNILKTCIGQAL
AFGNGKLTDDLTILILEIF
>Mature_579_residues
MNYYLFFPMAAFVANTMFIAFVYARRTNNPIIRSYLLYTISLDAWLFTYAFGWSFPPKPWMTWAFKILSITWLPLGTLYL
EFVFTFLNRKPGIFLWLFRIGIPISYLIILSTDWVIQGSIHYYWGNEGEPGPLYFFVIFNFVALPALIGLGVLTQSFFAS
GKDQRKQTGLAILGSMFAIFLSFYSEIIQVDDQGRMLSMPLTSIGIVIQSFLIFTAITRYGFLRINVEGLAADLFRDIHD
GMILVRRDQSLFFMNESAIRILGISNSPPSHFYPSDFLKGYRESPDHFSKEYEPIFNVDCRGVELTRSHIELTGKESGYL
YILRDISEKIESREKIERFYSDFNKDLEIAKIAQTSAISTIFPESSKYKFYSHFQPFELVGGDFLRALERSDGKLDILFA
DVSGHGISSAMVAGMLSISFQLVIETNPTPKKALEKTQTLLLNAVLNHHVSAIYLSFDPDTKTLEYSYAGHHPILIFREG
EIIPLRGVGRILLITPDTELNNYTFQLKKGDILFLYSDCLFEVRSPEGEILGYENFMLKMNEISVYTPVNILKTCIGQAL
AFGNGKLTDDLTILILEIF

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 66165; Mature: 66165

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYYLFFPMAAFVANTMFIAFVYARRTNNPIIRSYLLYTISLDAWLFTYAFGWSFPPKPW
CCEEEEEHHHHHHHHHHHHHHEEECCCCCHHHHHHEEEEEEHHHHHHHHHHCCCCCCCHH
MTWAFKILSITWLPLGTLYLEFVFTFLNRKPGIFLWLFRIGIPISYLIILSTDWVIQGSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCEEEEEEEECCEEEECCE
HYYWGNEGEPGPLYFFVIFNFVALPALIGLGVLTQSFFASGKDQRKQTGLAILGSMFAIF
EEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LSFYSEIIQVDDQGRMLSMPLTSIGIVIQSFLIFTAITRYGFLRINVEGLAADLFRDIHD
HHHHHHHHEECCCCCEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCC
GMILVRRDQSLFFMNESAIRILGISNSPPSHFYPSDFLKGYRESPDHFSKEYEPIFNVDC
CEEEEEECCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCC
RGVELTRSHIELTGKESGYLYILRDISEKIESREKIERFYSDFNKDLEIAKIAQTSAIST
CCEEEEECEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHE
IFPESSKYKFYSHFQPFELVGGDFLRALERSDGKLDILFADVSGHGISSAMVAGMLSISF
ECCCCCCEEEECCCCCHHHHCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHEEEE
QLVIETNPTPKKALEKTQTLLLNAVLNHHVSAIYLSFDPDTKTLEYSYAGHHPILIFREG
EEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEEECCCCCEEEEECC
EIIPLRGVGRILLITPDTELNNYTFQLKKGDILFLYSDCLFEVRSPEGEILGYENFMLKM
CEEEECCCCEEEEECCCCCCCCEEEEEECCCEEEEEECCHHEECCCCCCEEECCCEEEEE
NEISVYTPVNILKTCIGQALAFGNGKLTDDLTILILEIF
ECEEEECCHHHHHHHHHHHEECCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MNYYLFFPMAAFVANTMFIAFVYARRTNNPIIRSYLLYTISLDAWLFTYAFGWSFPPKPW
CCEEEEEHHHHHHHHHHHHHHEEECCCCCHHHHHHEEEEEEHHHHHHHHHHCCCCCCCHH
MTWAFKILSITWLPLGTLYLEFVFTFLNRKPGIFLWLFRIGIPISYLIILSTDWVIQGSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCCEEEEEEEECCEEEECCE
HYYWGNEGEPGPLYFFVIFNFVALPALIGLGVLTQSFFASGKDQRKQTGLAILGSMFAIF
EEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LSFYSEIIQVDDQGRMLSMPLTSIGIVIQSFLIFTAITRYGFLRINVEGLAADLFRDIHD
HHHHHHHHEECCCCCEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCC
GMILVRRDQSLFFMNESAIRILGISNSPPSHFYPSDFLKGYRESPDHFSKEYEPIFNVDC
CEEEEEECCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCC
RGVELTRSHIELTGKESGYLYILRDISEKIESREKIERFYSDFNKDLEIAKIAQTSAIST
CCEEEEECEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHE
IFPESSKYKFYSHFQPFELVGGDFLRALERSDGKLDILFADVSGHGISSAMVAGMLSISF
ECCCCCCEEEECCCCCHHHHCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHEEEE
QLVIETNPTPKKALEKTQTLLLNAVLNHHVSAIYLSFDPDTKTLEYSYAGHHPILIFREG
EEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEEECCCCCEEEEECC
EIIPLRGVGRILLITPDTELNNYTFQLKKGDILFLYSDCLFEVRSPEGEILGYENFMLKM
CEEEECCCCEEEEECCCCCCCCEEEEEECCCEEEEEECCHHEECCCCCCEEECCCEEEEE
NEISVYTPVNILKTCIGQALAFGNGKLTDDLTILILEIF
ECEEEECCHHHHHHHHHHHEECCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]