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The map label for this gene is trmA-2 [C]

Identifier: 116329018

GI number: 116329018

Start: 2872693

End: 2873874

Strand: Reverse

Name: trmA-2 [C]

Synonym: LBL_2422

Alternate gene names: 116329018

Gene position: 2873874-2872693 (Counterclockwise)

Preceding gene: 116329019

Following gene: 116329009

Centisome position: 79.51

GC content: 43.32

Gene sequence:

>1182_bases
ATGAGACCTCCCATGAGTCAGTCCTGTCAACATTATCCGGAATGCGCCGGTTGTGATCGATTGCATATCAGCTACGAAAA
ACAGCTGCAGCACAAACAAGAAGGGGTCGAAAAACGGTTCGTAGGTTTCCAAGAACTTGAAATCCGACCGATCATAAAAA
GTCCGAAGGAGCAGATGTATCGTCATAAGGTTCAACTTCCGTTCGGACACCGTAAGATCGGGAAAAAAACCGTTCTCACT
TTAGGACTTCATAATAAGGAGAACGTATATATTATCGATCAAAAAGAATGTAGAATTCAAGATGCGGACTTGACTACCGT
GGCCGCCGCGATCCGACATTGGGCGCGTACTGAAAACGTCATTCCCTATAATGAAAAAAACGGAAGCGGGCTTTTGAGAC
ATATCGTACTCAGAAAGGCGAACGCATCCCAGGAGATATTAGTGGGAATTGTGACGAACGCGACCGAAATCCCTGGAAGA
AAAAAACTTGCCGACAGATTACATTCATATATTCAACAATTCTTATGTAATGAGAAATCGAAAGCCGAGGTCGTGGGGAT
ACTGCAAAACGTAAATCAGAGAAATACGAAGGTAGTTTTGGGCGAAAAAGAAACGACTTGGTACGGAAGGCATTTTGTAA
AGGAAAAGATCGGTGATCTTGATTTTCAGATCGGACTTTCCACGTTTTTTCAAGTGAATCCGTTTCAGATTAAAAATCTT
TACGATCTCGTTTTGCAGGAACTTCCGAGCGATTCGAATGTAGTGGACGCTTATTGTGGAATAGGAACGATTTCCTTGTA
TGTTGCTTCCAAATCCAAAAAAGTGGTCGGACTGGAAGAAAATCCGAATTCGATCCGTTCCGCGGTTGGGGCCGCAAAGG
CGAACGGAATCGTAAACGTCGCGTTTACCAAGGGAAAGGTTTTACGAACTTTGCAGGTGGCTTTGAATGAAAAATCGGAC
GTAGTGATTTTGGATCCTCCGAGGGAAGGACTTGACCCTGAGACGAAAAAAATATTATTAAATTCTAAAATACATAAGAT
TCTTTATGTTTCTTGTAATCCGGAGACGCTGCTACGGGATGCGGTTGCACTCACCAAGGTCTTCAAATATACGAAGATGA
CTCCGGTGGATCTGTTCCCTCATACGAGCCATCTGGAAAGTGTTTCCGTTTTTACAAGGTGA

Upstream 100 bases:

>100_bases
CGACAATTAAGGACAAACACGGAAAGACTTATCTGATGTAAACTGCTTCAAGAAACGAATTCAATTGACAAGCCAGGGCC
TGAAAGTGGAAGAAATTAGA

Downstream 100 bases:

>100_bases
TTTCTTGCGGATCGGTTATTGAATGTGAAATTCGACCTAAAGAGCGCGTCTCAAAAAATAGAACTTATGTTAGACAACCT
TGGATCGATTTTACAAAGGG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MRPPMSQSCQHYPECAGCDRLHISYEKQLQHKQEGVEKRFVGFQELEIRPIIKSPKEQMYRHKVQLPFGHRKIGKKTVLT
LGLHNKENVYIIDQKECRIQDADLTTVAAAIRHWARTENVIPYNEKNGSGLLRHIVLRKANASQEILVGIVTNATEIPGR
KKLADRLHSYIQQFLCNEKSKAEVVGILQNVNQRNTKVVLGEKETTWYGRHFVKEKIGDLDFQIGLSTFFQVNPFQIKNL
YDLVLQELPSDSNVVDAYCGIGTISLYVASKSKKVVGLEENPNSIRSAVGAAKANGIVNVAFTKGKVLRTLQVALNEKSD
VVILDPPREGLDPETKKILLNSKIHKILYVSCNPETLLRDAVALTKVFKYTKMTPVDLFPHTSHLESVSVFTR

Sequences:

>Translated_393_residues
MRPPMSQSCQHYPECAGCDRLHISYEKQLQHKQEGVEKRFVGFQELEIRPIIKSPKEQMYRHKVQLPFGHRKIGKKTVLT
LGLHNKENVYIIDQKECRIQDADLTTVAAAIRHWARTENVIPYNEKNGSGLLRHIVLRKANASQEILVGIVTNATEIPGR
KKLADRLHSYIQQFLCNEKSKAEVVGILQNVNQRNTKVVLGEKETTWYGRHFVKEKIGDLDFQIGLSTFFQVNPFQIKNL
YDLVLQELPSDSNVVDAYCGIGTISLYVASKSKKVVGLEENPNSIRSAVGAAKANGIVNVAFTKGKVLRTLQVALNEKSD
VVILDPPREGLDPETKKILLNSKIHKILYVSCNPETLLRDAVALTKVFKYTKMTPVDLFPHTSHLESVSVFTR
>Mature_393_residues
MRPPMSQSCQHYPECAGCDRLHISYEKQLQHKQEGVEKRFVGFQELEIRPIIKSPKEQMYRHKVQLPFGHRKIGKKTVLT
LGLHNKENVYIIDQKECRIQDADLTTVAAAIRHWARTENVIPYNEKNGSGLLRHIVLRKANASQEILVGIVTNATEIPGR
KKLADRLHSYIQQFLCNEKSKAEVVGILQNVNQRNTKVVLGEKETTWYGRHFVKEKIGDLDFQIGLSTFFQVNPFQIKNL
YDLVLQELPSDSNVVDAYCGIGTISLYVASKSKKVVGLEENPNSIRSAVGAAKANGIVNVAFTKGKVLRTLQVALNEKSD
VVILDPPREGLDPETKKILLNSKIHKILYVSCNPETLLRDAVALTKVFKYTKMTPVDLFPHTSHLESVSVFTR

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=365, Percent_Identity=27.9452054794521, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI51173878, Length=365, Percent_Identity=27.9452054794521, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI269784680, Length=373, Percent_Identity=26.5415549597855, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI269784676, Length=373, Percent_Identity=26.5415549597855, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI20149688, Length=373, Percent_Identity=26.5415549597855, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI269784678, Length=205, Percent_Identity=31.219512195122, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789148, Length=391, Percent_Identity=27.3657289002558, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787083, Length=385, Percent_Identity=23.1168831168831, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790403, Length=158, Percent_Identity=32.9113924050633, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322909, Length=132, Percent_Identity=34.0909090909091, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24666579, Length=420, Percent_Identity=26.6666666666667, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 44434; Mature: 44434

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPMSQSCQHYPECAGCDRLHISYEKQLQHKQEGVEKRFVGFQELEIRPIIKSPKEQMY
CCCCCCCHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCHHHEEEEHHHCCCHHHHH
RHKVQLPFGHRKIGKKTVLTLGLHNKENVYIIDQKECRIQDADLTTVAAAIRHWARTENV
HHEECCCCCCHHCCCEEEEEEECCCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHCCCC
IPYNEKNGSGLLRHIVLRKANASQEILVGIVTNATEIPGRKKLADRLHSYIQQFLCNEKS
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
KAEVVGILQNVNQRNTKVVLGEKETTWYGRHFVKEKIGDLDFQIGLSTFFQVNPFQIKNL
HHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECHHHHEECCCHHHHHH
YDLVLQELPSDSNVVDAYCGIGTISLYVASKSKKVVGLEENPNSIRSAVGAAKANGIVNV
HHHHHHHCCCCCCEEEHHCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCEEEE
AFTKGKVLRTLQVALNEKSDVVILDPPREGLDPETKKILLNSKIHKILYVSCNPETLLRD
EECCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEEEEECCHHHHHHH
AVALTKVFKYTKMTPVDLFPHTSHLESVSVFTR
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MRPPMSQSCQHYPECAGCDRLHISYEKQLQHKQEGVEKRFVGFQELEIRPIIKSPKEQMY
CCCCCCCHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCHHHEEEEHHHCCCHHHHH
RHKVQLPFGHRKIGKKTVLTLGLHNKENVYIIDQKECRIQDADLTTVAAAIRHWARTENV
HHEECCCCCCHHCCCEEEEEEECCCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHCCCC
IPYNEKNGSGLLRHIVLRKANASQEILVGIVTNATEIPGRKKLADRLHSYIQQFLCNEKS
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
KAEVVGILQNVNQRNTKVVLGEKETTWYGRHFVKEKIGDLDFQIGLSTFFQVNPFQIKNL
HHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECHHHHEECCCHHHHHH
YDLVLQELPSDSNVVDAYCGIGTISLYVASKSKKVVGLEENPNSIRSAVGAAKANGIVNV
HHHHHHHCCCCCCEEEHHCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCEEEE
AFTKGKVLRTLQVALNEKSDVVILDPPREGLDPETKKILLNSKIHKILYVSCNPETLLRD
EECCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEEEEECCHHHHHHH
AVALTKVFKYTKMTPVDLFPHTSHLESVSVFTR
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA