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The map label for this gene is recQ-2 [H]

Identifier: 116328991

GI number: 116328991

Start: 2834015

End: 2835463

Strand: Reverse

Name: recQ-2 [H]

Synonym: LBL_2393

Alternate gene names: 116328991

Gene position: 2835463-2834015 (Counterclockwise)

Preceding gene: 116328999

Following gene: 116328986

Centisome position: 78.45

GC content: 41.06

Gene sequence:

>1449_bases
ATGCCTTCTCTTAGTCAGCTCGGAAACATTTTCGGAATTTCCACATTCAGAAGTTCTCAAGAGAGAATCATTTTGGACGT
TCTTTCCGGAAAGAATTGTCTGGTCGTCATGCCGACTGGAATGGGAAAGTCGATTTGTTATCAACTTCCTTCTCTTGCTT
TGGATGGGTTGACCGTTGTCATATCTCCTTTAATCGCGTTGATGCAGGATCAGGTTTTAAAATTGAAGCGACTTGGGATC
GACGCGGGTTATATTAATTCCTCTCTTTCCAAACAGGAAAAGCTTCGGTGTTATCGGGATTTGAAAGAAGGAAAGTACAA
AATCGTGTATGTTTCACCGGAACGATTTCGTAAGAAAGAATTTATAGATTCGCTTCAACTTAGAAAGGTATCTCTACTTG
CGATCGACGAAGCTCATTGTATCAGTCAGTGGGGGCACGATTTTAGACCGGACTATATGAAGATTGGGGAATTTCGGGAG
ATTCTAGGAAAACCGACTATAATAGCGTTAACCGCAACTGCAACTCTTGAGATTCAAAAAGACATTGTGCTTCAAATGAA
TTTACGGGAATCGGAAATTCAAGTTTATAACGAAGGAATCTGCAGACCCAATCTGTTTCTGGACGTTCGAACTTTTGTAG
ATGAACCTTCCAAGTCGGATGCGATTCTTGAACTTTTGAAAAAACAAAAAAAGAGTACAATCGTTTATTTTAATTTGATT
CGGAGTTTGGAAAAGTTTGGAGAGAAATTGGATCTACAAAAAGTTCCGTATAAAATCTATCACGGACGTTTGTCCCCCGA
GCAAAGAAAAAAAGTTCAAAGACAATTTTTAGAGTCGGAAGATACGATTTTACTAGCGACAAATGCATTCGGGATGGGAG
TCGATAAACCGAATATACGAATGATCATACACGCGGAACTTCCTTCGTCTTTGGAGGGGTATTACCAGGAAATCGGAAGA
GCGGGGAGGGACGGGGATCCCTCCGATTGTCACGTATTTTACGACCAAGACGATTTGACCGTCTTGATGGATTTTATCGA
GTGGCAAAATCCGGATGCATCTTTTATTGCTCGTACCTACCAAACTATGGAACGACTTGGAGAAAAATTGTCTTCGATCG
AATACGACGAATTGCAGTCTATGATCGTTCATAAAAATCGAAGAGATCATCGGCTTCAAACCGTTCTCAATTTGTTTGAA
AGGCACGGAGTTACATCGGGAGAGTTGGAAAAAAAGTCGCTGAAATTGAGATCCCCGTTGCCGGATGTGCTTTGCTCTTC
CGAATATTTGGAACGAAAAAAGAAAACGAGTTTGAAACGATTGTATCAAATGTTTCTTTATCTGAAATCGGAAAGATGCA
GAAGGGAGTTCGTGTACGAATACTTCGATGCTAAATGGAGTGGGTGCGGGAACTGCGACGTCTGTAAATATCGGACAACG
AGAGTATGA

Upstream 100 bases:

>100_bases
CTACTCCGACCTTGAATACGAACATTCTAAGAATTAGGCATAGTGGCAAGTAATTACGTTTTCTCTTGCTTTCAAAGACC
CAAGATAAATTTTAATCGTA

Downstream 100 bases:

>100_bases
AAATAGACCGGTTTATATTCGTATCTGATTTAAAGCCGCGTCAAAAACGACTTTTCGATCGAAGGTCCGTGTGTCGCCTA
CGACCGAAATTTCTTGACTT

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MPSLSQLGNIFGISTFRSSQERIILDVLSGKNCLVVMPTGMGKSICYQLPSLALDGLTVVISPLIALMQDQVLKLKRLGI
DAGYINSSLSKQEKLRCYRDLKEGKYKIVYVSPERFRKKEFIDSLQLRKVSLLAIDEAHCISQWGHDFRPDYMKIGEFRE
ILGKPTIIALTATATLEIQKDIVLQMNLRESEIQVYNEGICRPNLFLDVRTFVDEPSKSDAILELLKKQKKSTIVYFNLI
RSLEKFGEKLDLQKVPYKIYHGRLSPEQRKKVQRQFLESEDTILLATNAFGMGVDKPNIRMIIHAELPSSLEGYYQEIGR
AGRDGDPSDCHVFYDQDDLTVLMDFIEWQNPDASFIARTYQTMERLGEKLSSIEYDELQSMIVHKNRRDHRLQTVLNLFE
RHGVTSGELEKKSLKLRSPLPDVLCSSEYLERKKKTSLKRLYQMFLYLKSERCRREFVYEYFDAKWSGCGNCDVCKYRTT
RV

Sequences:

>Translated_482_residues
MPSLSQLGNIFGISTFRSSQERIILDVLSGKNCLVVMPTGMGKSICYQLPSLALDGLTVVISPLIALMQDQVLKLKRLGI
DAGYINSSLSKQEKLRCYRDLKEGKYKIVYVSPERFRKKEFIDSLQLRKVSLLAIDEAHCISQWGHDFRPDYMKIGEFRE
ILGKPTIIALTATATLEIQKDIVLQMNLRESEIQVYNEGICRPNLFLDVRTFVDEPSKSDAILELLKKQKKSTIVYFNLI
RSLEKFGEKLDLQKVPYKIYHGRLSPEQRKKVQRQFLESEDTILLATNAFGMGVDKPNIRMIIHAELPSSLEGYYQEIGR
AGRDGDPSDCHVFYDQDDLTVLMDFIEWQNPDASFIARTYQTMERLGEKLSSIEYDELQSMIVHKNRRDHRLQTVLNLFE
RHGVTSGELEKKSLKLRSPLPDVLCSSEYLERKKKTSLKRLYQMFLYLKSERCRREFVYEYFDAKWSGCGNCDVCKYRTT
RV
>Mature_481_residues
PSLSQLGNIFGISTFRSSQERIILDVLSGKNCLVVMPTGMGKSICYQLPSLALDGLTVVISPLIALMQDQVLKLKRLGID
AGYINSSLSKQEKLRCYRDLKEGKYKIVYVSPERFRKKEFIDSLQLRKVSLLAIDEAHCISQWGHDFRPDYMKIGEFREI
LGKPTIIALTATATLEIQKDIVLQMNLRESEIQVYNEGICRPNLFLDVRTFVDEPSKSDAILELLKKQKKSTIVYFNLIR
SLEKFGEKLDLQKVPYKIYHGRLSPEQRKKVQRQFLESEDTILLATNAFGMGVDKPNIRMIIHAELPSSLEGYYQEIGRA
GRDGDPSDCHVFYDQDDLTVLMDFIEWQNPDASFIARTYQTMERLGEKLSSIEYDELQSMIVHKNRRDHRLQTVLNLFER
HGVTSGELEKKSLKLRSPLPDVLCSSEYLERKKKTSLKRLYQMFLYLKSERCRREFVYEYFDAKWSGCGNCDVCKYRTTR
V

Specific function: Probable DNA helicase. Required for DNA repair and intramolecular recombination; probably has overlapping function with recS (AC P50729). It probably acts to help generate ss-DNA from ds-DNA breaks [H]

COG id: COG0514

COG function: function code L; Superfamily II DNA helicase

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Localized throughout the nucleoid in the presence or absence of DNA double- strand breaks [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HRDC domain [H]

Homologues:

Organism=Homo sapiens, GI51242947, Length=351, Percent_Identity=40.7407407407407, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI51242943, Length=351, Percent_Identity=40.7407407407407, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI148612816, Length=351, Percent_Identity=40.7407407407407, Blast_Score=258, Evalue=9e-69,
Organism=Homo sapiens, GI14591902, Length=357, Percent_Identity=38.0952380952381, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI14591904, Length=357, Percent_Identity=38.0952380952381, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI4557365, Length=347, Percent_Identity=41.2103746397695, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI110735439, Length=344, Percent_Identity=39.2441860465116, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI169218196, Length=344, Percent_Identity=39.2441860465116, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI284005309, Length=355, Percent_Identity=34.6478873239437, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI21071032, Length=368, Percent_Identity=25.8152173913043, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI148613856, Length=345, Percent_Identity=24.0579710144928, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI38201710, Length=345, Percent_Identity=24.0579710144928, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI4758138, Length=341, Percent_Identity=24.9266862170088, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI7661920, Length=355, Percent_Identity=21.9718309859155, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI48994977, Length=395, Percent_Identity=39.2405063291139, Blast_Score=281, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI25145561, Length=354, Percent_Identity=40.6779661016949, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI32564293, Length=349, Percent_Identity=38.6819484240688, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17552866, Length=357, Percent_Identity=37.2549019607843, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71987997, Length=343, Percent_Identity=38.4839650145773, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71987993, Length=343, Percent_Identity=38.4839650145773, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI115533224, Length=353, Percent_Identity=25.4957507082153, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71995724, Length=357, Percent_Identity=23.8095238095238, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17507121, Length=357, Percent_Identity=23.5294117647059, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71987143, Length=345, Percent_Identity=22.0289855072464, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323844, Length=361, Percent_Identity=35.7340720221607, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321360, Length=333, Percent_Identity=26.7267267267267, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324217, Length=328, Percent_Identity=22.8658536585366, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320224, Length=349, Percent_Identity=22.6361031518625, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325138, Length=140, Percent_Identity=27.1428571428571, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324778, Length=225, Percent_Identity=24.4444444444444, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24646066, Length=342, Percent_Identity=39.4736842105263, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24664230, Length=355, Percent_Identity=38.0281690140845, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI62472181, Length=355, Percent_Identity=38.0281690140845, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24664226, Length=359, Percent_Identity=37.3259052924791, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI21358123, Length=369, Percent_Identity=34.9593495934959, Blast_Score=215, Evalue=7e-56,
Organism=Drosophila melanogaster, GI24644483, Length=369, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24644487, Length=369, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45551833, Length=369, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24644485, Length=369, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24644479, Length=369, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI281361218, Length=369, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24644481, Length=369, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28574962, Length=346, Percent_Identity=22.8323699421965, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24584399, Length=343, Percent_Identity=22.7405247813411, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004589
- InterPro:   IPR006293
- InterPro:   IPR002121
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR010997
- InterPro:   IPR018982 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF00570 HRDC; PF09382 RQC [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55805; Mature: 55674

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLSQLGNIFGISTFRSSQERIILDVLSGKNCLVVMPTGMGKSICYQLPSLALDGLTVV
CCCHHHHHHHHEEHHHCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
ISPLIALMQDQVLKLKRLGIDAGYINSSLSKQEKLRCYRDLKEGKYKIVYVSPERFRKKE
HHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHH
FIDSLQLRKVSLLAIDEAHCISQWGHDFRPDYMKIGEFREILGKPTIIALTATATLEIQK
HHHHHHHHHEEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEEC
DIVLQMNLRESEIQVYNEGICRPNLFLDVRTFVDEPSKSDAILELLKKQKKSTIVYFNLI
CEEEEECCCHHHHHHHHCCCCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHCEEHHHHHH
RSLEKFGEKLDLQKVPYKIYHGRLSPEQRKKVQRQFLESEDTILLATNAFGMGVDKPNIR
HHHHHHHHHCCHHHCCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEE
MIIHAELPSSLEGYYQEIGRAGRDGDPSDCHVFYDQDDLTVLMDFIEWQNPDASFIARTY
EEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHH
QTMERLGEKLSSIEYDELQSMIVHKNRRDHRLQTVLNLFERHGVTSGELEKKSLKLRSPL
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCC
PDVLCSSEYLERKKKTSLKRLYQMFLYLKSERCRREFVYEYFDAKWSGCGNCDVCKYRTT
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCC
RV
CC
>Mature Secondary Structure 
PSLSQLGNIFGISTFRSSQERIILDVLSGKNCLVVMPTGMGKSICYQLPSLALDGLTVV
CCHHHHHHHHEEHHHCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
ISPLIALMQDQVLKLKRLGIDAGYINSSLSKQEKLRCYRDLKEGKYKIVYVSPERFRKKE
HHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHH
FIDSLQLRKVSLLAIDEAHCISQWGHDFRPDYMKIGEFREILGKPTIIALTATATLEIQK
HHHHHHHHHEEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEEC
DIVLQMNLRESEIQVYNEGICRPNLFLDVRTFVDEPSKSDAILELLKKQKKSTIVYFNLI
CEEEEECCCHHHHHHHHCCCCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHCEEHHHHHH
RSLEKFGEKLDLQKVPYKIYHGRLSPEQRKKVQRQFLESEDTILLATNAFGMGVDKPNIR
HHHHHHHHHCCHHHCCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEE
MIIHAELPSSLEGYYQEIGRAGRDGDPSDCHVFYDQDDLTVLMDFIEWQNPDASFIARTY
EEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHH
QTMERLGEKLSSIEYDELQSMIVHKNRRDHRLQTVLNLFERHGVTSGELEKKSLKLRSPL
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCC
PDVLCSSEYLERKKKTSLKRLYQMFLYLKSERCRREFVYEYFDAKWSGCGNCDVCKYRTT
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCC
RV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9642195 [H]