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The map label for this gene is pldB-2 [H]

Identifier: 116328820

GI number: 116328820

Start: 2588195

End: 2589142

Strand: Reverse

Name: pldB-2 [H]

Synonym: LBL_2198

Alternate gene names: 116328820

Gene position: 2589142-2588195 (Counterclockwise)

Preceding gene: 116328823

Following gene: 116328819

Centisome position: 71.63

GC content: 40.19

Gene sequence:

>948_bases
ATGACCTTTCATCACAAAGAATTTTACATCTTATCTGGTTCCGATAAATCAAAATTGTACTGCCAATCCTGGACGAAGCC
AAACTCCAATCGATTGGTAATCTTTCACCACGGTTTTGGAGAACATAGCGGGCGTTATGCAAACTTGCTCCGTTATTTTG
CAGGAAGCGATATCAATTTCTATTCTTTCGATATGAGAGGTCACGGTAATTCAGAGGGAAAAAGAGGACATGCGGATTCT
TTCGATTTATATGTGAGAGATCTGGCGAATTTCGTTTCCGAAGTTTTCAAAAGAGAAGGAAAGGAGCGTTTTTTTCTTTT
AGGACATTCGCTCGGAGCAGCCGTTGCGCTTCGATATTCTCAGGAGGGAATTAATCAGGACAATATTTTGGGTCTGATTT
TAGGTTCTCCTGCGCTCTCGGTAAAAATGGATTTTAAAAAGAGGCTTAAAAATTTTTCGGTCAGTTTGTTAAGTAAAGTT
TCTCCTTCTCTCACCGTGGACGCGGAATTGGATTTTCAATATCTTTCCCATGATCCGGATGTGATCGAGGCCTACAAACA
GGATCCTCTCGTTCATGGAACGATTTCCCTCAAGATGGGAAGCGAGCTTTTGGAAATCGGGCCTAAACTGATTAAAAAAG
CGAATGTGCTTCGATGCCCGGTTTTGATTCTTCACGGCCAAGAAGACGGGCTTGTGGATGTGAACGGTTCTACGGAACTT
TATAAAAATCTAATTTATAGAAATAAAAGAATCAAAATTTATCCCGGACTTTATCACGAATTGATGAACGAATTTCCGGA
ACATAGAGATGTGGTGCTAAACGATATCCAGACGTTTTTAGAAACCATTCAGAGAGAAAAGGTGGAGGATCCTTCTTTAA
AGATGAAAAAGGAAATTCCGACTTCACCGAAAAAGAGAAACGAGAAAAACGCGGTTTCGAATCTTTAA

Upstream 100 bases:

>100_bases
TGTCACGATGAAAAAAAACCATTTTTGATCAGTTCTATAATATTTAGTTGACCCGTATCGTTTTAATGATTCTTTTCTCC
CAAATAAATCGGTATTAAAA

Downstream 100 bases:

>100_bases
CGTTCTCGAATTTTTTGTTTGTTCTTGGAAATGTTTTTGGATGTGAGTTTTCGGTATTCATTTCCGATTTTTGAATATTT
TTTGCGGTAAATTCAGGCTG

Product: lysophospholipase

Products: NA

Alternate protein names: Bacilysocin biosynthesis protein ytpA [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTFHHKEFYILSGSDKSKLYCQSWTKPNSNRLVIFHHGFGEHSGRYANLLRYFAGSDINFYSFDMRGHGNSEGKRGHADS
FDLYVRDLANFVSEVFKREGKERFFLLGHSLGAAVALRYSQEGINQDNILGLILGSPALSVKMDFKKRLKNFSVSLLSKV
SPSLTVDAELDFQYLSHDPDVIEAYKQDPLVHGTISLKMGSELLEIGPKLIKKANVLRCPVLILHGQEDGLVDVNGSTEL
YKNLIYRNKRIKIYPGLYHELMNEFPEHRDVVLNDIQTFLETIQREKVEDPSLKMKKEIPTSPKKRNEKNAVSNL

Sequences:

>Translated_315_residues
MTFHHKEFYILSGSDKSKLYCQSWTKPNSNRLVIFHHGFGEHSGRYANLLRYFAGSDINFYSFDMRGHGNSEGKRGHADS
FDLYVRDLANFVSEVFKREGKERFFLLGHSLGAAVALRYSQEGINQDNILGLILGSPALSVKMDFKKRLKNFSVSLLSKV
SPSLTVDAELDFQYLSHDPDVIEAYKQDPLVHGTISLKMGSELLEIGPKLIKKANVLRCPVLILHGQEDGLVDVNGSTEL
YKNLIYRNKRIKIYPGLYHELMNEFPEHRDVVLNDIQTFLETIQREKVEDPSLKMKKEIPTSPKKRNEKNAVSNL
>Mature_314_residues
TFHHKEFYILSGSDKSKLYCQSWTKPNSNRLVIFHHGFGEHSGRYANLLRYFAGSDINFYSFDMRGHGNSEGKRGHADSF
DLYVRDLANFVSEVFKREGKERFFLLGHSLGAAVALRYSQEGINQDNILGLILGSPALSVKMDFKKRLKNFSVSLLSKVS
PSLTVDAELDFQYLSHDPDVIEAYKQDPLVHGTISLKMGSELLEIGPKLIKKANVLRCPVLILHGQEDGLVDVNGSTELY
KNLIYRNKRIKIYPGLYHELMNEFPEHRDVVLNDIQTFLETIQREKVEDPSLKMKKEIPTSPKKRNEKNAVSNL

Specific function: Phospholipase involved in the biosynthesis of the antibiotic bacilysocin. It probably catalyzes the hydrolysis of the 2-sn-acyl moiety of phosphatidylglycerol to produce bacilysocin (lysophosphatidylglycerol). Is also able to catalyze the hydrolysis react

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI51242953, Length=272, Percent_Identity=31.6176470588235, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI6005786, Length=272, Percent_Identity=31.6176470588235, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI1787678, Length=285, Percent_Identity=26.3157894736842, Blast_Score=119, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322756, Length=266, Percent_Identity=27.0676691729323, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022742 [H]

Pfam domain/function: PF12146 Hydrolase_4 [H]

EC number: NA

Molecular weight: Translated: 35967; Mature: 35835

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFHHKEFYILSGSDKSKLYCQSWTKPNSNRLVIFHHGFGEHSGRYANLLRYFAGSDINF
CCCEEEEEEEEECCCCCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEE
YSFDMRGHGNSEGKRGHADSFDLYVRDLANFVSEVFKREGKERFFLLGHSLGAAVALRYS
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHEEEEEH
QEGINQDNILGLILGSPALSVKMDFKKRLKNFSVSLLSKVSPSLTVDAELDFQYLSHDPD
HCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCH
VIEAYKQDPLVHGTISLKMGSELLEIGPKLIKKANVLRCPVLILHGQEDGLVDVNGSTEL
HHHHHHCCCEEEEEEEEECCHHHHHHCHHHHHHCCCEECCEEEEECCCCCEEEECCCHHH
YKNLIYRNKRIKIYPGLYHELMNEFPEHRDVVLNDIQTFLETIQREKVEDPSLKMKKEIP
HHHHHHCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
TSPKKRNEKNAVSNL
CCCCCCCCCHHHCCC
>Mature Secondary Structure 
TFHHKEFYILSGSDKSKLYCQSWTKPNSNRLVIFHHGFGEHSGRYANLLRYFAGSDINF
CCEEEEEEEEECCCCCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEE
YSFDMRGHGNSEGKRGHADSFDLYVRDLANFVSEVFKREGKERFFLLGHSLGAAVALRYS
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHEEEEEH
QEGINQDNILGLILGSPALSVKMDFKKRLKNFSVSLLSKVSPSLTVDAELDFQYLSHDPD
HCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCH
VIEAYKQDPLVHGTISLKMGSELLEIGPKLIKKANVLRCPVLILHGQEDGLVDVNGSTEL
HHHHHHCCCEEEEEEEEECCHHHHHHCHHHHHHCCCEECCEEEEECCCCCEEEECCCHHH
YKNLIYRNKRIKIYPGLYHELMNEFPEHRDVVLNDIQTFLETIQREKVEDPSLKMKKEIP
HHHHHHCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
TSPKKRNEKNAVSNL
CCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]