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The map label for this gene is lpdA-1 [H]

Identifier: 116328747

GI number: 116328747

Start: 2482439

End: 2483842

Strand: Reverse

Name: lpdA-1 [H]

Synonym: LBL_2121

Alternate gene names: 116328747

Gene position: 2483842-2482439 (Counterclockwise)

Preceding gene: 116328748

Following gene: 116328746

Centisome position: 68.72

GC content: 50.0

Gene sequence:

>1404_bases
ATGTCAGCAGAATTCGACGTAGTCGTGATCGGTGCGGGACCAGGGGGCTACGTTTGCGCCATCCGGGCCGCTCAACTCGG
TTTCAAAACCGCAATCATCGAAAAAAGAAAAACTCTCGGAGGCACCTGCCTCAATGTGGGTTGTATTCCTTCCAAAGCTC
TTTTGGACTCGTCCGAAGAATACCACAAAGTTCTGCATAAACTCGACGTTCACGGAATCACAGTCGGTAAAGTAGAAGTC
GATCTCGGCAAACTGATGAACCGCAAGGATCAGATCGTAAAGGAAGTCACCGACGGCGTGGACTTTCTGATCGGCAAAAA
TAAGATCAAACGGTACGAAGGTTTCGGAAAAGTTTTATCCGCTGGAAAAGTCGAAGTCGCATCGAGCGGCGGGGATAAAG
AAGTAATCAACGCAAAACATATCGTAGTCGCAACTGGGTCGGTTCCGATCGACATTCCCGGTTTGACTGTGGATGGAAAA
AACATCATCACATCCGATCACGCGATCGAACTCCGTAAACTTCCTAAAAAGATGATCATCATCGGTGCAGGAGTGATCGG
TCTCGAACTCGGATCGGTTTGGGCAAGACTCGGAACCGCGGTGACGGTCGTCGAATTTTTGCCCGGACTGATTTCGAACG
TGGACCGCCCGATGGGTGCACTTTTAGAACGTTCTCTTACTTCCCAGGGGATTGAGTTTTTATTCGAACACAAGGTCAAG
AGCGCGACTACGTCAAAGAACGGAGTGAAAGTTCAAATCGAAGATTCCAAAGGCGCAACTAAGGATCTCGAAGCGGACGT
GGTTCTTGTCGCCGTTGGAAGAAGGCCTTTTCTCGAAGGAGTGGGTTTGGAAGAGGCGGGTGTCGCATTAACACCCCGCA
ACCGAATCCAAGTGGACGGACATTTCAAAACGTCCGTTCCGGGAATCTATGCGATTGGGGATGCGATCGACGGACCGATG
CTTGCGCACAAAGCGGAAGAAGAAGGAGTAGCTTTGGCCGAACTTCTGGCGGGACAATCCGGACACGTTAACTACGATGC
GGTTCCTTACGTCATATATACTTGGCCGGAAATGGCTTGGGTAGGAAAAGGCGAGGAAGAATTGAAGGCGGCTGGAATTG
AATATAAAACCGGAAAATCGCTCTTTCGACCAAACGCACGCTCGAAAGCGATGAACGAAGCCGAAGGACAAGTCAAAATA
TTAGCGGATAAAAAAACGGATAAGCTTTTAGGAGCTTTTATATTCGGGCCAAGGGCTTCGGATATGATCGCGGAGTTGGC
GGTTGCGATGGAATTTGGGGCCTCCGCGGAAGATATAGCGAGGAGTTTTCATGCGCATCCGACTTTGGCGGAAGTGATTA
AAGAAGCGGCAATGGCGGTGGATAAGTGGGCGATTCACGCGTGA

Upstream 100 bases:

>100_bases
ATCGTAGACGGGAAAGAAGCGGTTACATTTCTCGTAAAGGTCAAAGAAGCGATCGAAGATCCGGCCCGACTTTTACTCGA
ACTTTAATGGAAGTGGAAAC

Downstream 100 bases:

>100_bases
ATCAGGTAGGTTTTAAGCAATGAAAGTCGAAAAACTCATGGCGCTTTATGGAGAGAACGGCGTTCTCCTCGAAGAACTTT
ATAATCAGTACAAACTCAAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSAEFDVVVIGAGPGGYVCAIRAAQLGFKTAIIEKRKTLGGTCLNVGCIPSKALLDSSEEYHKVLHKLDVHGITVGKVEV
DLGKLMNRKDQIVKEVTDGVDFLIGKNKIKRYEGFGKVLSAGKVEVASSGGDKEVINAKHIVVATGSVPIDIPGLTVDGK
NIITSDHAIELRKLPKKMIIIGAGVIGLELGSVWARLGTAVTVVEFLPGLISNVDRPMGALLERSLTSQGIEFLFEHKVK
SATTSKNGVKVQIEDSKGATKDLEADVVLVAVGRRPFLEGVGLEEAGVALTPRNRIQVDGHFKTSVPGIYAIGDAIDGPM
LAHKAEEEGVALAELLAGQSGHVNYDAVPYVIYTWPEMAWVGKGEEELKAAGIEYKTGKSLFRPNARSKAMNEAEGQVKI
LADKKTDKLLGAFIFGPRASDMIAELAVAMEFGASAEDIARSFHAHPTLAEVIKEAAMAVDKWAIHA

Sequences:

>Translated_467_residues
MSAEFDVVVIGAGPGGYVCAIRAAQLGFKTAIIEKRKTLGGTCLNVGCIPSKALLDSSEEYHKVLHKLDVHGITVGKVEV
DLGKLMNRKDQIVKEVTDGVDFLIGKNKIKRYEGFGKVLSAGKVEVASSGGDKEVINAKHIVVATGSVPIDIPGLTVDGK
NIITSDHAIELRKLPKKMIIIGAGVIGLELGSVWARLGTAVTVVEFLPGLISNVDRPMGALLERSLTSQGIEFLFEHKVK
SATTSKNGVKVQIEDSKGATKDLEADVVLVAVGRRPFLEGVGLEEAGVALTPRNRIQVDGHFKTSVPGIYAIGDAIDGPM
LAHKAEEEGVALAELLAGQSGHVNYDAVPYVIYTWPEMAWVGKGEEELKAAGIEYKTGKSLFRPNARSKAMNEAEGQVKI
LADKKTDKLLGAFIFGPRASDMIAELAVAMEFGASAEDIARSFHAHPTLAEVIKEAAMAVDKWAIHA
>Mature_466_residues
SAEFDVVVIGAGPGGYVCAIRAAQLGFKTAIIEKRKTLGGTCLNVGCIPSKALLDSSEEYHKVLHKLDVHGITVGKVEVD
LGKLMNRKDQIVKEVTDGVDFLIGKNKIKRYEGFGKVLSAGKVEVASSGGDKEVINAKHIVVATGSVPIDIPGLTVDGKN
IITSDHAIELRKLPKKMIIIGAGVIGLELGSVWARLGTAVTVVEFLPGLISNVDRPMGALLERSLTSQGIEFLFEHKVKS
ATTSKNGVKVQIEDSKGATKDLEADVVLVAVGRRPFLEGVGLEEAGVALTPRNRIQVDGHFKTSVPGIYAIGDAIDGPML
AHKAEEEGVALAELLAGQSGHVNYDAVPYVIYTWPEMAWVGKGEEELKAAGIEYKTGKSLFRPNARSKAMNEAEGQVKIL
ADKKTDKLLGAFIFGPRASDMIAELAVAMEFGASAEDIARSFHAHPTLAEVIKEAAMAVDKWAIHA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=51.304347826087, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=28.0777537796976, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI22035672, Length=455, Percent_Identity=30.989010989011, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI33519430, Length=456, Percent_Identity=26.3157894736842, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI33519428, Length=456, Percent_Identity=26.3157894736842, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI33519426, Length=456, Percent_Identity=26.3157894736842, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI148277065, Length=456, Percent_Identity=26.3157894736842, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI148277071, Length=456, Percent_Identity=26.3157894736842, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI291045266, Length=474, Percent_Identity=27.4261603375527, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI291045268, Length=467, Percent_Identity=25.6959314775161, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1786307, Length=461, Percent_Identity=38.177874186551, Blast_Score=311, Evalue=8e-86,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=26.8980477223427, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081717, Length=465, Percent_Identity=25.8064516129032, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=27.4599542334096, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789065, Length=240, Percent_Identity=27.5, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=50.7658643326039, Blast_Score=457, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17557007, Length=481, Percent_Identity=28.4823284823285, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI71982272, Length=480, Percent_Identity=26.0416666666667, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=454, Percent_Identity=26.8722466960352, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=454, Percent_Identity=26.8722466960352, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=252, Percent_Identity=25.3968253968254, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=47.7987421383648, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=32.4152542372881, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=29.0598290598291, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI21358499, Length=460, Percent_Identity=50, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17737741, Length=475, Percent_Identity=30.3157894736842, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24640553, Length=483, Percent_Identity=29.1925465838509, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=29.399585921325, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=29.1925465838509, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49745; Mature: 49614

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEFDVVVIGAGPGGYVCAIRAAQLGFKTAIIEKRKTLGGTCLNVGCIPSKALLDSSEE
CCCCEEEEEEECCCCCEEEEEEHHHHCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCHH
YHKVLHKLDVHGITVGKVEVDLGKLMNRKDQIVKEVTDGVDFLIGKNKIKRYEGFGKVLS
HHHHHHHHCCCCEEEEEEEECHHHHCCHHHHHHHHHHCCHHEECCCHHHHHHHHHHHHHC
AGKVEVASSGGDKEVINAKHIVVATGSVPIDIPGLTVDGKNIITSDHAIELRKLPKKMII
CCCEEEECCCCCCEEECCCEEEEEECCCEEECCCEEECCCCEECCCCHHHHHHCCCEEEE
IGAGVIGLELGSVWARLGTAVTVVEFLPGLISNVDRPMGALLERSLTSQGIEFLFEHKVK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SATTSKNGVKVQIEDSKGATKDLEADVVLVAVGRRPFLEGVGLEEAGVALTPRNRIQVDG
HCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEEEECC
HFKTSVPGIYAIGDAIDGPMLAHKAEEEGVALAELLAGQSGHVNYDAVPYVIYTWPEMAW
CEECCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCEEE
VGKGEEELKAAGIEYKTGKSLFRPNARSKAMNEAEGQVKILADKKTDKLLGAFIFGPRAS
CCCCHHHHHHCCCCCCCCCHHHCCCHHHHHHHCCCCCEEEEECCCCHHHHHHEEECCCHH
DMIAELAVAMEFGASAEDIARSFHAHPTLAEVIKEAAMAVDKWAIHA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAEFDVVVIGAGPGGYVCAIRAAQLGFKTAIIEKRKTLGGTCLNVGCIPSKALLDSSEE
CCCEEEEEEECCCCCEEEEEEHHHHCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCHH
YHKVLHKLDVHGITVGKVEVDLGKLMNRKDQIVKEVTDGVDFLIGKNKIKRYEGFGKVLS
HHHHHHHHCCCCEEEEEEEECHHHHCCHHHHHHHHHHCCHHEECCCHHHHHHHHHHHHHC
AGKVEVASSGGDKEVINAKHIVVATGSVPIDIPGLTVDGKNIITSDHAIELRKLPKKMII
CCCEEEECCCCCCEEECCCEEEEEECCCEEECCCEEECCCCEECCCCHHHHHHCCCEEEE
IGAGVIGLELGSVWARLGTAVTVVEFLPGLISNVDRPMGALLERSLTSQGIEFLFEHKVK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SATTSKNGVKVQIEDSKGATKDLEADVVLVAVGRRPFLEGVGLEEAGVALTPRNRIQVDG
HCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEEEECC
HFKTSVPGIYAIGDAIDGPMLAHKAEEEGVALAELLAGQSGHVNYDAVPYVIYTWPEMAW
CEECCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCEEE
VGKGEEELKAAGIEYKTGKSLFRPNARSKAMNEAEGQVKILADKKTDKLLGAFIFGPRAS
CCCCHHHHHHCCCCCCCCCHHHCCCHHHHHHHCCCCCEEEEECCCCHHHHHHEEECCCHH
DMIAELAVAMEFGASAEDIARSFHAHPTLAEVIKEAAMAVDKWAIHA
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]