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The map label for this gene is thrS

Identifier: 116328741

GI number: 116328741

Start: 2473523

End: 2475442

Strand: Reverse

Name: thrS

Synonym: LBL_2115

Alternate gene names: 116328741

Gene position: 2475442-2473523 (Counterclockwise)

Preceding gene: 116328744

Following gene: 116328739

Centisome position: 68.49

GC content: 45.62

Gene sequence:

>1920_bases
ATGTATCAGCTGACACTGCCGGACAAATCAGTAAAAAAGGTCGCATTAGGCTCCACTTATCGAGACTTCATCGAAAAAGA
GTTACCCTTTTTAAAAAACAAAGCCCTTGCCGTACGCTTGAACGGCAAGGATATTCAGGACTTATCCCGAGTGGTGGAAA
CAGACGCAAACATAGAAGTATTGACTTACACCGAAAAAGCGGGTTGGGAAACCTTTCAACATTCCGCGGCGCACTTGCTC
GGGATGGCGGTTCAGAATTTATATAAGAATGCAAATCTTACCGTGGGTCCCGTGATCGAGAACGGTCCGGGATTTTTTTA
TTACGACATCGATTTTCAAGGTACGATCATCACGCCCGAAGATTTTCCGAAGATCGAAGCGGAGATGGAAAGGATCGTAA
AAGCGGACTACACGGTTTGGAGAAAGGTCGTTCCGAAAAAAGAGGCGATCGAAACCTTTCAAAAACTCGGAGAGAAGTAT
AAGATCGAAATCATCGGAGGAATTCTGAGCGAGGAAGTTTCCATCTACGGAATGGGAGAATGGTTCGACTTATGCCGAGG
ACCGCACGTTTCGAACTCGGGGATATTAAAATCTTTTAAACTAACCGCGATTTCCGGCGCGTATTGGAAAGCCGACAAGG
ACAACGCGATGCTCACCCGTATCTACGGAGTCGCGTTTCCGAGTAAGAAAGAACTGGATCAGTATCTTTTTCAAATCGAA
GAAGCGAAAAAAAGGGATCACAGAAAGATCGGAAAGGAACTGGATCTATTCTCCTTTCAGAAGGAAGGTCCGGGATTTCC
TTTCTGGCATCCGAAGGGAACCATCCTTTGGAATTCACTCGCTGATTATCTTAGAGCGGAATGCAACAAACGCGGTTATC
AGGAAATCAAAACTCCCGCGGTTCTTTCTTCCGAACTTTGGAAGAAGTCTGGTCACTGGGATAACTTTCACGAAAACATG
TATTTTACGGACATCGACGAAGAGGACTACGCACTCAAGCCGATGAATTGTCCGGGATGTTCCTTAATCTACAAACATCA
CCTGCATTCGTATCGGGAACTTCCTCTTCGATTCGCCGAGTTCGGAAGCGTTCACCGTCACGAGCTGCACGGAGTGCTTC
ACGGACTTTTTAGAGTGAGGGCTTTTACTCAGGACGATTCCCATATCTACGCGCCTTTGGAACATCTGGAATCCGAGGTG
ACGGATATCATCGACTTTACGTTTACCGTATATAAGAAATTCGGATTTTCCGAATTCAAAACCTTCATTGCGACCCGTCC
GGAAAAATCGCAAGGAAGGGACGAAGACTGGGAGTTTGCGACAAATACCTTAAAACAATCTCTTGAGAAAAAAGGAATCC
CATACGGAATCAAAGAGGGAGAAGGTGCGTTTTACGGACCCAAAATAGAGTTTAACATCAAGGATTCGATCGGAAGGCTC
TGGCAGTGCGGAACGATCCAAGTCGACTTTTCCATGCCCGAACGTTTCGAATTGGATTACACCGACAGCGACGGTCAGAA
AAAAAGGCCGGTTATGATTCACAGGGCGATCTATGGTTCCTTGGAGCGATTTATCGGAATTCTAATCGAACACTACGAAG
GAAAATTTCCTCTTTGGATTTCACCGAACCAGATAAGGATATTGACGATTACCGAAAAGGTGGCCGAATACGCGAAAGAC
GTCTATTGCGAGTTAGTCGATGCCGGATTTCGTGTGGAATTGGATACACGCAACGAAAAGATCGGCGCCAAGATTCGGGA
TTCTATTTTAAAAAAGGCGAATTATCTTTTGATTCTCGGTGAAAAGGAAATGGAATCCGGGACGTTGGCTGTCAGAAAAC
GCGGTCAAGAGGACACGAAAACTTTGACTCGTTCGGGATTCATTTCCAATCTGCAAGACGAAATCAAATCGGCAGGTTAG

Upstream 100 bases:

>100_bases
CGATCGATCTTCGGCTTTGTGAGCAATCGGTGCAAAGCTTTGAGTTTATAGCCGAATTCGTTCTAACGGGAAAAATAGAA
TTTTAGGATAAGTCGTGAAT

Downstream 100 bases:

>100_bases
GTGAGTGGATCTTCCGATATGGTTCTACGAAACAAACCCGGACATATATAAGGAGAGAAGTGGATTTGTAAGGTGACGTT
CTCCCGGATATTGAAACTGC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MYQLTLPDKSVKKVALGSTYRDFIEKELPFLKNKALAVRLNGKDIQDLSRVVETDANIEVLTYTEKAGWETFQHSAAHLL
GMAVQNLYKNANLTVGPVIENGPGFFYYDIDFQGTIITPEDFPKIEAEMERIVKADYTVWRKVVPKKEAIETFQKLGEKY
KIEIIGGILSEEVSIYGMGEWFDLCRGPHVSNSGILKSFKLTAISGAYWKADKDNAMLTRIYGVAFPSKKELDQYLFQIE
EAKKRDHRKIGKELDLFSFQKEGPGFPFWHPKGTILWNSLADYLRAECNKRGYQEIKTPAVLSSELWKKSGHWDNFHENM
YFTDIDEEDYALKPMNCPGCSLIYKHHLHSYRELPLRFAEFGSVHRHELHGVLHGLFRVRAFTQDDSHIYAPLEHLESEV
TDIIDFTFTVYKKFGFSEFKTFIATRPEKSQGRDEDWEFATNTLKQSLEKKGIPYGIKEGEGAFYGPKIEFNIKDSIGRL
WQCGTIQVDFSMPERFELDYTDSDGQKKRPVMIHRAIYGSLERFIGILIEHYEGKFPLWISPNQIRILTITEKVAEYAKD
VYCELVDAGFRVELDTRNEKIGAKIRDSILKKANYLLILGEKEMESGTLAVRKRGQEDTKTLTRSGFISNLQDEIKSAG

Sequences:

>Translated_639_residues
MYQLTLPDKSVKKVALGSTYRDFIEKELPFLKNKALAVRLNGKDIQDLSRVVETDANIEVLTYTEKAGWETFQHSAAHLL
GMAVQNLYKNANLTVGPVIENGPGFFYYDIDFQGTIITPEDFPKIEAEMERIVKADYTVWRKVVPKKEAIETFQKLGEKY
KIEIIGGILSEEVSIYGMGEWFDLCRGPHVSNSGILKSFKLTAISGAYWKADKDNAMLTRIYGVAFPSKKELDQYLFQIE
EAKKRDHRKIGKELDLFSFQKEGPGFPFWHPKGTILWNSLADYLRAECNKRGYQEIKTPAVLSSELWKKSGHWDNFHENM
YFTDIDEEDYALKPMNCPGCSLIYKHHLHSYRELPLRFAEFGSVHRHELHGVLHGLFRVRAFTQDDSHIYAPLEHLESEV
TDIIDFTFTVYKKFGFSEFKTFIATRPEKSQGRDEDWEFATNTLKQSLEKKGIPYGIKEGEGAFYGPKIEFNIKDSIGRL
WQCGTIQVDFSMPERFELDYTDSDGQKKRPVMIHRAIYGSLERFIGILIEHYEGKFPLWISPNQIRILTITEKVAEYAKD
VYCELVDAGFRVELDTRNEKIGAKIRDSILKKANYLLILGEKEMESGTLAVRKRGQEDTKTLTRSGFISNLQDEIKSAG
>Mature_639_residues
MYQLTLPDKSVKKVALGSTYRDFIEKELPFLKNKALAVRLNGKDIQDLSRVVETDANIEVLTYTEKAGWETFQHSAAHLL
GMAVQNLYKNANLTVGPVIENGPGFFYYDIDFQGTIITPEDFPKIEAEMERIVKADYTVWRKVVPKKEAIETFQKLGEKY
KIEIIGGILSEEVSIYGMGEWFDLCRGPHVSNSGILKSFKLTAISGAYWKADKDNAMLTRIYGVAFPSKKELDQYLFQIE
EAKKRDHRKIGKELDLFSFQKEGPGFPFWHPKGTILWNSLADYLRAECNKRGYQEIKTPAVLSSELWKKSGHWDNFHENM
YFTDIDEEDYALKPMNCPGCSLIYKHHLHSYRELPLRFAEFGSVHRHELHGVLHGLFRVRAFTQDDSHIYAPLEHLESEV
TDIIDFTFTVYKKFGFSEFKTFIATRPEKSQGRDEDWEFATNTLKQSLEKKGIPYGIKEGEGAFYGPKIEFNIKDSIGRL
WQCGTIQVDFSMPERFELDYTDSDGQKKRPVMIHRAIYGSLERFIGILIEHYEGKFPLWISPNQIRILTITEKVAEYAKD
VYCELVDAGFRVELDTRNEKIGAKIRDSILKKANYLLILGEKEMESGTLAVRKRGQEDTKTLTRSGFISNLQDEIKSAG

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=634, Percent_Identity=41.1671924290221, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI38570111, Length=614, Percent_Identity=40.3908794788274, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI20070344, Length=634, Percent_Identity=36.4353312302839, Blast_Score=409, Evalue=1e-114,
Organism=Escherichia coli, GI1788013, Length=639, Percent_Identity=44.7574334898279, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1786392, Length=185, Percent_Identity=26.4864864864865, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71984192, Length=619, Percent_Identity=40.8723747980614, Blast_Score=496, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71984184, Length=619, Percent_Identity=40.8723747980614, Blast_Score=496, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6322112, Length=636, Percent_Identity=39.937106918239, Blast_Score=472, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322654, Length=395, Percent_Identity=38.4810126582278, Blast_Score=286, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24583839, Length=633, Percent_Identity=39.9684044233807, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24583842, Length=633, Percent_Identity=39.9684044233807, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI28574141, Length=633, Percent_Identity=39.9684044233807, Blast_Score=494, Evalue=1e-140,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_LEPBJ (Q04R58)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801370.1
- ProteinModelPortal:   Q04R58
- SMR:   Q04R58
- STRING:   Q04R58
- GeneID:   4410371
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2118
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   FHPKGMV
- PhylomeDB:   Q04R58
- ProtClustDB:   PRK00413
- BioCyc:   LBOR355277:LBJ_2118-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 73576; Mature: 73576

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQLTLPDKSVKKVALGSTYRDFIEKELPFLKNKALAVRLNGKDIQDLSRVVETDANIEV
CEEEECCCHHHHHHHCCCHHHHHHHHHCCHHCCCEEEEEECCCCHHHHHHHHHCCCCEEE
LTYTEKAGWETFQHSAAHLLGMAVQNLYKNANLTVGPVIENGPGFFYYDIDFQGTIITPE
EEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECEEECCCCEEEEEEEEEEEEECCC
DFPKIEAEMERIVKADYTVWRKVVPKKEAIETFQKLGEKYKIEIIGGILSEEVSIYGMGE
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECHHCCCEEEEECCH
WFDLCRGPHVSNSGILKSFKLTAISGAYWKADKDNAMLTRIYGVAFPSKKELDQYLFQIE
HHHHHCCCCCCCCCCCEEEEEEEECCCEECCCCCCCEEEHHHHCCCCCHHHHHHHHHHHH
EAKKRDHRKIGKELDLFSFQKEGPGFPFWHPKGTILWNSLADYLRAECNKRGYQEIKTPA
HHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHCCCH
VLSSELWKKSGHWDNFHENMYFTDIDEEDYALKPMNCPGCSLIYKHHLHSYRELPLRFAE
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHCCHHHHH
FGSVHRHELHGVLHGLFRVRAFTQDDSHIYAPLEHLESEVTDIIDFTFTVYKKFGFSEFK
HCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
TFIATRPEKSQGRDEDWEFATNTLKQSLEKKGIPYGIKEGEGAFYGPKIEFNIKDSIGRL
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCEEEEEEHHHHHHH
WQCGTIQVDFSMPERFELDYTDSDGQKKRPVMIHRAIYGSLERFIGILIEHYEGKFPLWI
EECCEEEEECCCCCEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEE
SPNQIRILTITEKVAEYAKDVYCELVDAGFRVELDTRNEKIGAKIRDSILKKANYLLILG
CCCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEE
EKEMESGTLAVRKRGQEDTKTLTRSGFISNLQDEIKSAG
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYQLTLPDKSVKKVALGSTYRDFIEKELPFLKNKALAVRLNGKDIQDLSRVVETDANIEV
CEEEECCCHHHHHHHCCCHHHHHHHHHCCHHCCCEEEEEECCCCHHHHHHHHHCCCCEEE
LTYTEKAGWETFQHSAAHLLGMAVQNLYKNANLTVGPVIENGPGFFYYDIDFQGTIITPE
EEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECEEECCCCEEEEEEEEEEEEECCC
DFPKIEAEMERIVKADYTVWRKVVPKKEAIETFQKLGEKYKIEIIGGILSEEVSIYGMGE
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECHHCCCEEEEECCH
WFDLCRGPHVSNSGILKSFKLTAISGAYWKADKDNAMLTRIYGVAFPSKKELDQYLFQIE
HHHHHCCCCCCCCCCCEEEEEEEECCCEECCCCCCCEEEHHHHCCCCCHHHHHHHHHHHH
EAKKRDHRKIGKELDLFSFQKEGPGFPFWHPKGTILWNSLADYLRAECNKRGYQEIKTPA
HHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHCCCH
VLSSELWKKSGHWDNFHENMYFTDIDEEDYALKPMNCPGCSLIYKHHLHSYRELPLRFAE
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHCCHHHHH
FGSVHRHELHGVLHGLFRVRAFTQDDSHIYAPLEHLESEVTDIIDFTFTVYKKFGFSEFK
HCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
TFIATRPEKSQGRDEDWEFATNTLKQSLEKKGIPYGIKEGEGAFYGPKIEFNIKDSIGRL
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCEEEEEEHHHHHHH
WQCGTIQVDFSMPERFELDYTDSDGQKKRPVMIHRAIYGSLERFIGILIEHYEGKFPLWI
EECCEEEEECCCCCEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEE
SPNQIRILTITEKVAEYAKDVYCELVDAGFRVELDTRNEKIGAKIRDSILKKANYLLILG
CCCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEE
EKEMESGTLAVRKRGQEDTKTLTRSGFISNLQDEIKSAG
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA