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The map label for this gene is nusA [H]

Identifier: 116328714

GI number: 116328714

Start: 2446953

End: 2448341

Strand: Reverse

Name: nusA [H]

Synonym: LBL_2088

Alternate gene names: 116328714

Gene position: 2448341-2446953 (Counterclockwise)

Preceding gene: 116328715

Following gene: 116328713

Centisome position: 67.74

GC content: 46.87

Gene sequence:

>1389_bases
ATGGCAGTTAAGAAGACACAATCGGAAGGAAATCTGTTGGAAGTGATTCAACAATTTTGCGCGGACAAGTCCCTCGATCG
GGAAGCCGTTATGGGAGTCATTCGAGATTCTCTCATAACCGCATACAAGAAGAAGTCCGGCCTCGAAGGTTTGGAAGAAT
TCGAAGAATCGGAAAAACCTTCTCCGGTTACGGTAGAGTTCGCTTCCGGTAAAGACAGCGTAGTGATTGCAATCGCTAAA
AAAGTCGTAGAGGGAACTCCTTCATCCGCTCTCGAAATCGGTTTAGAAGATGCTAAAGCGATCGACGCATCCGCAGAAAT
CGGATCCGTCATTTCGTTCCGTGAAAAACCGGTGGAACTTTCCAGAATTATTTCCAGCCAAGCGAAACAAATGGTCTTCC
AAAGATTAAAAGATATGGAGAAAGAACTTCTCTATAACGAGTATAAGGCGAAGGAAGGGGAGCTTACTCACGGTTATTTC
CAGAGATGGAAAAAAGACGCGATGAGCATCGATCTTGGAAAAGTAGAAGGGATCATGCCTCGTCGTGAACAGAATCCGGG
TGAAAAATATCACAGTGGGGATCGACTCAAAGCAATCATTCAAAGAGTGGAACTTCGGCCAAGAGAACCAATTCCCGTAA
TCACTCTTTCCCGCGCCTCCGCGGATTTTGTCCGTAAACTTTTCGAAATGGAAATCCCTGAAATTTATGACGGACTCGTA
GAAATCATCAATGTAGCCAGGCAACCTTCCATTCGGACTAAAGTTGTAGTGCGTGCAACCCGTGGAGAGATCGATCCTGT
GGGTGCTTGTGTGGGAATGAAGGGAGTTCGGATTCAATCGATCGTAAGGGAGTTGGGAAACGAGAGAATTGATATCGTCG
AGGCTTCCGACGACGCGTCCGAGTTTATTGCAAACGCGATTTCTCCTGCAAAACCGGTAGAGGTCAAAGTGGACGGATCC
GGTAGGGAAGCGATGGTAGTCGTTCCGGACGATCAACTTTCTCTTGCGATCGGAATCAACGGATCCAATGTGAAACTTGC
TTCTCAGTTAGCGGGCTACAAAATCGATATCAAAACAGTGGCTCAATATAATGCAGAACTTGCGTCTCCGGAAGCGAGAG
AAAGGTTGGAAAGGCTGTTCTATTCTCCTGCGGAAGAAGCGAAAGTTGCGGCTCCACAAGAGGAAGAAGAATTGACTCCT
CTGGAAGATCTTCCCGGCCTCTCGGCTCGTATTGTCGGGATTTTGAAGAGCGAGGGAATTAAAGATCTGGAAACCCTGAT
CGAATACAGTCAAGACGATCTGGCAAAACTACAAGGAATTGGACATACGACCGCCGGACAGATTCTCAAGTTACTTCGAG
AATCCGTAGAATGGGTGGAGGATAACTGA

Upstream 100 bases:

>100_bases
CTGGAAAAATTCCAAAAGGGAAAGAAATCTGCCGTAAAAAAGCAGACGACCCTGAACCTCAAGGATATACTGAAAGGGAA
TCTTTACGTAAGTATTTGAT

Downstream 100 bases:

>100_bases
AAAAACCCGAATCGAGGGTGATTCAGAGCTGAGGTTTTTAGGAACTTCGGAAAGCCAGGATATCAATTATGGAAGATAAA
AATAAGACGATCAAGGAAAC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MAVKKTQSEGNLLEVIQQFCADKSLDREAVMGVIRDSLITAYKKKSGLEGLEEFEESEKPSPVTVEFASGKDSVVIAIAK
KVVEGTPSSALEIGLEDAKAIDASAEIGSVISFREKPVELSRIISSQAKQMVFQRLKDMEKELLYNEYKAKEGELTHGYF
QRWKKDAMSIDLGKVEGIMPRREQNPGEKYHSGDRLKAIIQRVELRPREPIPVITLSRASADFVRKLFEMEIPEIYDGLV
EIINVARQPSIRTKVVVRATRGEIDPVGACVGMKGVRIQSIVRELGNERIDIVEASDDASEFIANAISPAKPVEVKVDGS
GREAMVVVPDDQLSLAIGINGSNVKLASQLAGYKIDIKTVAQYNAELASPEARERLERLFYSPAEEAKVAAPQEEEELTP
LEDLPGLSARIVGILKSEGIKDLETLIEYSQDDLAKLQGIGHTTAGQILKLLRESVEWVEDN

Sequences:

>Translated_462_residues
MAVKKTQSEGNLLEVIQQFCADKSLDREAVMGVIRDSLITAYKKKSGLEGLEEFEESEKPSPVTVEFASGKDSVVIAIAK
KVVEGTPSSALEIGLEDAKAIDASAEIGSVISFREKPVELSRIISSQAKQMVFQRLKDMEKELLYNEYKAKEGELTHGYF
QRWKKDAMSIDLGKVEGIMPRREQNPGEKYHSGDRLKAIIQRVELRPREPIPVITLSRASADFVRKLFEMEIPEIYDGLV
EIINVARQPSIRTKVVVRATRGEIDPVGACVGMKGVRIQSIVRELGNERIDIVEASDDASEFIANAISPAKPVEVKVDGS
GREAMVVVPDDQLSLAIGINGSNVKLASQLAGYKIDIKTVAQYNAELASPEARERLERLFYSPAEEAKVAAPQEEEELTP
LEDLPGLSARIVGILKSEGIKDLETLIEYSQDDLAKLQGIGHTTAGQILKLLRESVEWVEDN
>Mature_461_residues
AVKKTQSEGNLLEVIQQFCADKSLDREAVMGVIRDSLITAYKKKSGLEGLEEFEESEKPSPVTVEFASGKDSVVIAIAKK
VVEGTPSSALEIGLEDAKAIDASAEIGSVISFREKPVELSRIISSQAKQMVFQRLKDMEKELLYNEYKAKEGELTHGYFQ
RWKKDAMSIDLGKVEGIMPRREQNPGEKYHSGDRLKAIIQRVELRPREPIPVITLSRASADFVRKLFEMEIPEIYDGLVE
IINVARQPSIRTKVVVRATRGEIDPVGACVGMKGVRIQSIVRELGNERIDIVEASDDASEFIANAISPAKPVEVKVDGSG
REAMVVVPDDQLSLAIGINGSNVKLASQLAGYKIDIKTVAQYNAELASPEARERLERLFYSPAEEAKVAAPQEEEELTPL
EDLPGLSARIVGILKSEGIKDLETLIEYSQDDLAKLQGIGHTTAGQILKLLRESVEWVEDN

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=504, Percent_Identity=30.1587301587302, Blast_Score=205, Evalue=4e-54,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 50973; Mature: 50842

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKKTQSEGNLLEVIQQFCADKSLDREAVMGVIRDSLITAYKKKSGLEGLEEFEESEKP
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
SPVTVEFASGKDSVVIAIAKKVVEGTPSSALEIGLEDAKAIDASAEIGSVISFREKPVEL
CCEEEEECCCCCCEEHHHHHHHHCCCCCHHHHHCCHHHHHHCCHHHHHHHHHHHCCCHHH
SRIISSQAKQMVFQRLKDMEKELLYNEYKAKEGELTHGYFQRWKKDAMSIDLGKVEGIMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEHHHHHCCCC
RREQNPGEKYHSGDRLKAIIQRVELRPREPIPVITLSRASADFVRKLFEMEIPEIYDGLV
CCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHH
EIINVARQPSIRTKVVVRATRGEIDPVGACVGMKGVRIQSIVRELGNERIDIVEASDDAS
HHHHHHCCCCCCEEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCHH
EFIANAISPAKPVEVKVDGSGREAMVVVPDDQLSLAIGINGSNVKLASQLAGYKIDIKTV
HHHHHHCCCCCCEEEEECCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHCCCEEEHHHH
AQYNAELASPEARERLERLFYSPAEEAKVAAPQEEEELTPLEDLPGLSARIVGILKSEGI
HHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCHHCCCCHHHCCCCHHHHHHHHHHCCH
KDLETLIEYSQDDLAKLQGIGHTTAGQILKLLRESVEWVEDN
HHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVKKTQSEGNLLEVIQQFCADKSLDREAVMGVIRDSLITAYKKKSGLEGLEEFEESEKP
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
SPVTVEFASGKDSVVIAIAKKVVEGTPSSALEIGLEDAKAIDASAEIGSVISFREKPVEL
CCEEEEECCCCCCEEHHHHHHHHCCCCCHHHHHCCHHHHHHCCHHHHHHHHHHHCCCHHH
SRIISSQAKQMVFQRLKDMEKELLYNEYKAKEGELTHGYFQRWKKDAMSIDLGKVEGIMP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEHHHHHCCCC
RREQNPGEKYHSGDRLKAIIQRVELRPREPIPVITLSRASADFVRKLFEMEIPEIYDGLV
CCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHH
EIINVARQPSIRTKVVVRATRGEIDPVGACVGMKGVRIQSIVRELGNERIDIVEASDDAS
HHHHHHCCCCCCEEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCHH
EFIANAISPAKPVEVKVDGSGREAMVVVPDDQLSLAIGINGSNVKLASQLAGYKIDIKTV
HHHHHHCCCCCCEEEEECCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHCCCEEEHHHH
AQYNAELASPEARERLERLFYSPAEEAKVAAPQEEEELTPLEDLPGLSARIVGILKSEGI
HHHCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCHHCCCCHHHCCCCHHHHHHHHHHCCH
KDLETLIEYSQDDLAKLQGIGHTTAGQILKLLRESVEWVEDN
HHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]