Click here to switch to the map view.

The map label for this gene is pnp [H]

Identifier: 116328709

GI number: 116328709

Start: 2440633

End: 2442723

Strand: Reverse

Name: pnp [H]

Synonym: LBL_2083

Alternate gene names: 116328709

Gene position: 2442723-2440633 (Counterclockwise)

Preceding gene: 116328710

Following gene: 116328708

Centisome position: 67.58

GC content: 44.57

Gene sequence:

>2091_bases
GTGACGCATACAATTTCCGGCCAATATGGCCGAGATACGATCGTTCTCGAAACTGGAAACTGGGCGAAACAAGCTCACGG
CGCAGTCGTTTATAAATCCGGAAACCTAGTATTACTTGCGACCGTTTGTGCCGCCGATGATGCGAAAGAAGGACAAGATT
TTTTCCCTCTTACTTGTGAATATACTGAAAAACTCTATTCCGTTGGTCGTTTTCCCGGTGGCTATTTTAAAAGGGAAGCT
AAACCTCCGGAGCATGAAATTCTTATTTCCAGAATTATAGACAGACCGATTCGACCTCTGTTCCCTGAGGGATACTTCTG
CGAAGTGCAACTTCAAGTCCAGGTTCTTTCCGCGGACGGAGACGTTTCTGTTGCAGGACACGCGCTGAATGCCGCCAGCG
TTGCATTAACGATTTCTGATATTCCTTTCAACGGACCGATTGCAGGTGCGAGAATCGGAAGAATCAACGGAGAGTTGATT
TTGAATCCGACTACGAAGGAAATTGTGAACTCCGATTTGGATCTGGTTGTTGCCGGAACTAAAACGCATATCGTCATGAT
TGAGGGAGAAGCGAAAGAGCTGAGCAACGAAGAAATGCTTTCGGCTCTTCGTTTCGCTCAAAAACATATCGCCGAATTTG
TGACTCTTCAGGAAGAATATGCTAAACAAATCGGAGTCGTCAAACGAGAAGTTAAATTAAAAGCCCGGGACGTTGAACTT
CTTGCTAAGGTAAAAGAATATGCGTTTGCAAAACTAACCGCCGCAAATCAAACTCCCGACAAAACCGTTCGTAATAAAGA
AATCTCTAACGTGAACAAAGACGTTGTCGAGTTCTTCAAGCAAACGGTGGAAGATGCGGACAAGATCAAAGATATAAAAA
CATATCTGCACGAATTGGAATACGAAATCGTGCGTGAACAAGTTCTGAACCAAGGAGTTCGTTTTGACGGAAGAAGATTA
GACGAAATTCGTCCTATTTCCGTGGAAATCAATCCGCTTCCCGGCCCTCACGGTTCCTCCGTTTTTACTAGGGGGCAGAC
TCAATCTTTAGGGGTTGTTACTTTAGGAACGGGATCCGACAATCAGAGATACGAAACTTTGGAAGGTCAAAAAGAGAAGT
CCTTCATGTTGCATTATAACTTTCCTGCGTTTTCTGTGGGTGAGGTTCGTAGATCTTCCGGTCCCGGAAGAAGGGAAATC
GGACATGGAAATTTGGCGGAACGAGCTCTCAAATTAGTTCTTCCTAAGTCGGAAGAATTTCCGTATGTGATCCGAGTTGT
ATCCGAAATTTTAGAGTCCAACGGTTCCAGTTCTATGGCTTCCGTTTGTTCCGGTTCTTTGGCACTGATGGCGGCGGGTG
TTCCGATTAAAGGAAGCGTTGCCGGAATTGCGATGGGACTTTTTTCGGATTCTTCCGGTAAGTATGCGGTGTTATCCGAT
ATCGCCGGTTTGGAAGATCATTTCGGAGACATGGATTGTAAGATTGCAGGAACCAGAAAAGGGATCACCGCGTTTCAGAT
GGATTTGAAAGTGACCGGTGTCAGTTTCGATGTTTTAGAAAGTGTATTCGAACAGGCACAAAGGGGAAGATTCCATATCT
TGGATATTATGGAAAAACATATCTCCAAGGCTTCCGATAGTTTAGCCGGAACCGCTCCTAGAATCATCGTTCGAAATATA
CCTAAGGACAGAATCGGCGAGCTCATCGGTCCGGGTGGTAAAAACGTTCGAGGAATCAGTGAACTTACAGGAGCGGAGCT
CTATATAGAAGACGACGGAAGAGTGACCATCTCCGGCTCCAATCAAGAGTCCGCAGAAAAAGCAGCGAAGATGGTGGATG
GATTTTTCGCAGAGGTCGAAGTAGGAAAGATATACGAAGGAAAAGTAAAACGGATTGCGGATTTCGGAGCGTTTGTCGAG
ATCCTTCCCGGTAAAGAAGGTCTTTGTCATATCTCTAAGATCGATTTTAAAAGAGTAAATTCCGTGAAAGATATCGTCAA
AGAAGGTGATATCATTCGAGTAAAGGTTTTGAACGTGGATAAGACTGGAAAGATCGATCTTTCCAGAAAAGACGCTCTTG
AGGAAATATAA

Upstream 100 bases:

>100_bases
TTTTAAGACTCGTAGGCAAAAGAAAGAAACTTCTGGATTATCTCAAAAGAACCGAATTAGATCGTTATAAAAAACTAATT
GAAACTCTCGGACTTCGTAA

Downstream 100 bases:

>100_bases
TATTTTTTTAATATTCCCTTGGTACAGGAATCTTCACAGTTAGTTTATAGAAAAGTTCTACCCGGGGGAATTACTCTCCT
TTTTCAACAAGCTCCTCATA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MTHTISGQYGRDTIVLETGNWAKQAHGAVVYKSGNLVLLATVCAADDAKEGQDFFPLTCEYTEKLYSVGRFPGGYFKREA
KPPEHEILISRIIDRPIRPLFPEGYFCEVQLQVQVLSADGDVSVAGHALNAASVALTISDIPFNGPIAGARIGRINGELI
LNPTTKEIVNSDLDLVVAGTKTHIVMIEGEAKELSNEEMLSALRFAQKHIAEFVTLQEEYAKQIGVVKREVKLKARDVEL
LAKVKEYAFAKLTAANQTPDKTVRNKEISNVNKDVVEFFKQTVEDADKIKDIKTYLHELEYEIVREQVLNQGVRFDGRRL
DEIRPISVEINPLPGPHGSSVFTRGQTQSLGVVTLGTGSDNQRYETLEGQKEKSFMLHYNFPAFSVGEVRRSSGPGRREI
GHGNLAERALKLVLPKSEEFPYVIRVVSEILESNGSSSMASVCSGSLALMAAGVPIKGSVAGIAMGLFSDSSGKYAVLSD
IAGLEDHFGDMDCKIAGTRKGITAFQMDLKVTGVSFDVLESVFEQAQRGRFHILDIMEKHISKASDSLAGTAPRIIVRNI
PKDRIGELIGPGGKNVRGISELTGAELYIEDDGRVTISGSNQESAEKAAKMVDGFFAEVEVGKIYEGKVKRIADFGAFVE
ILPGKEGLCHISKIDFKRVNSVKDIVKEGDIIRVKVLNVDKTGKIDLSRKDALEEI

Sequences:

>Translated_696_residues
MTHTISGQYGRDTIVLETGNWAKQAHGAVVYKSGNLVLLATVCAADDAKEGQDFFPLTCEYTEKLYSVGRFPGGYFKREA
KPPEHEILISRIIDRPIRPLFPEGYFCEVQLQVQVLSADGDVSVAGHALNAASVALTISDIPFNGPIAGARIGRINGELI
LNPTTKEIVNSDLDLVVAGTKTHIVMIEGEAKELSNEEMLSALRFAQKHIAEFVTLQEEYAKQIGVVKREVKLKARDVEL
LAKVKEYAFAKLTAANQTPDKTVRNKEISNVNKDVVEFFKQTVEDADKIKDIKTYLHELEYEIVREQVLNQGVRFDGRRL
DEIRPISVEINPLPGPHGSSVFTRGQTQSLGVVTLGTGSDNQRYETLEGQKEKSFMLHYNFPAFSVGEVRRSSGPGRREI
GHGNLAERALKLVLPKSEEFPYVIRVVSEILESNGSSSMASVCSGSLALMAAGVPIKGSVAGIAMGLFSDSSGKYAVLSD
IAGLEDHFGDMDCKIAGTRKGITAFQMDLKVTGVSFDVLESVFEQAQRGRFHILDIMEKHISKASDSLAGTAPRIIVRNI
PKDRIGELIGPGGKNVRGISELTGAELYIEDDGRVTISGSNQESAEKAAKMVDGFFAEVEVGKIYEGKVKRIADFGAFVE
ILPGKEGLCHISKIDFKRVNSVKDIVKEGDIIRVKVLNVDKTGKIDLSRKDALEEI
>Mature_695_residues
THTISGQYGRDTIVLETGNWAKQAHGAVVYKSGNLVLLATVCAADDAKEGQDFFPLTCEYTEKLYSVGRFPGGYFKREAK
PPEHEILISRIIDRPIRPLFPEGYFCEVQLQVQVLSADGDVSVAGHALNAASVALTISDIPFNGPIAGARIGRINGELIL
NPTTKEIVNSDLDLVVAGTKTHIVMIEGEAKELSNEEMLSALRFAQKHIAEFVTLQEEYAKQIGVVKREVKLKARDVELL
AKVKEYAFAKLTAANQTPDKTVRNKEISNVNKDVVEFFKQTVEDADKIKDIKTYLHELEYEIVREQVLNQGVRFDGRRLD
EIRPISVEINPLPGPHGSSVFTRGQTQSLGVVTLGTGSDNQRYETLEGQKEKSFMLHYNFPAFSVGEVRRSSGPGRREIG
HGNLAERALKLVLPKSEEFPYVIRVVSEILESNGSSSMASVCSGSLALMAAGVPIKGSVAGIAMGLFSDSSGKYAVLSDI
AGLEDHFGDMDCKIAGTRKGITAFQMDLKVTGVSFDVLESVFEQAQRGRFHILDIMEKHISKASDSLAGTAPRIIVRNIP
KDRIGELIGPGGKNVRGISELTGAELYIEDDGRVTISGSNQESAEKAAKMVDGFFAEVEVGKIYEGKVKRIADFGAFVEI
LPGKEGLCHISKIDFKRVNSVKDIVKEGDIIRVKVLNVDKTGKIDLSRKDALEEI

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=712, Percent_Identity=36.6573033707865, Blast_Score=435, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=688, Percent_Identity=46.5116279069767, Blast_Score=606, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI115534063, Length=705, Percent_Identity=35.0354609929078, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI281362905, Length=709, Percent_Identity=37.2355430183357, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651641, Length=709, Percent_Identity=37.2355430183357, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651643, Length=709, Percent_Identity=37.2355430183357, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161079377, Length=653, Percent_Identity=37.9785604900459, Blast_Score=404, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76173; Mature: 76042

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHTISGQYGRDTIVLETGNWAKQAHGAVVYKSGNLVLLATVCAADDAKEGQDFFPLTCE
CCCCCCCCCCCCEEEEECCCCHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCEEHH
YTEKLYSVGRFPGGYFKREAKPPEHEILISRIIDRPIRPLFPEGYFCEVQLQVQVLSADG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCC
DVSVAGHALNAASVALTISDIPFNGPIAGARIGRINGELILNPTTKEIVNSDLDLVVAGT
CEEEECCEECCEEEEEEEECCCCCCCCCCCEEEECCCEEEECCCHHHHCCCCCCEEEECC
KTHIVMIEGEAKELSNEEMLSALRFAQKHIAEFVTLQEEYAKQIGVVKREVKLKARDVEL
CEEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKVKEYAFAKLTAANQTPDKTVRNKEISNVNKDVVEFFKQTVEDADKIKDIKTYLHELE
HHHHHHHHHHEEEECCCCCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEIVREQVLNQGVRFDGRRLDEIRPISVEINPLPGPHGSSVFTRGQTQSLGVVTLGTGSD
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCEEEEECCCCC
NQRYETLEGQKEKSFMLHYNFPAFSVGEVRRSSGPGRREIGHGNLAERALKLVLPKSEEF
CCCCHHCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
PYVIRVVSEILESNGSSSMASVCSGSLALMAAGVPIKGSVAGIAMGLFSDSSGKYAVLSD
CHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCHHHEEEEEEECCCCCEEEHHH
IAGLEDHFGDMDCKIAGTRKGITAFQMDLKVTGVSFDVLESVFEQAQRGRFHILDIMEKH
HCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCEEHHHHHHHH
ISKASDSLAGTAPRIIVRNIPKDRIGELIGPGGKNVRGISELTGAELYIEDDGRVTISGS
HHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCCCCCCCHHHCCCCEEEEECCCEEEEECC
NQESAEKAAKMVDGFFAEVEVGKIYEGKVKRIADFGAFVEILPGKEGLCHISKIDFKRVN
CHHHHHHHHHHHHHHHHEEEECCEECCHHHHHHHHCCCEEECCCCCCCEEEECCCHHHHH
SVKDIVKEGDIIRVKVLNVDKTGKIDLSRKDALEEI
HHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
THTISGQYGRDTIVLETGNWAKQAHGAVVYKSGNLVLLATVCAADDAKEGQDFFPLTCE
CCCCCCCCCCCEEEEECCCCHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCEEHH
YTEKLYSVGRFPGGYFKREAKPPEHEILISRIIDRPIRPLFPEGYFCEVQLQVQVLSADG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCC
DVSVAGHALNAASVALTISDIPFNGPIAGARIGRINGELILNPTTKEIVNSDLDLVVAGT
CEEEECCEECCEEEEEEEECCCCCCCCCCCEEEECCCEEEECCCHHHHCCCCCCEEEECC
KTHIVMIEGEAKELSNEEMLSALRFAQKHIAEFVTLQEEYAKQIGVVKREVKLKARDVEL
CEEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKVKEYAFAKLTAANQTPDKTVRNKEISNVNKDVVEFFKQTVEDADKIKDIKTYLHELE
HHHHHHHHHHEEEECCCCCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEIVREQVLNQGVRFDGRRLDEIRPISVEINPLPGPHGSSVFTRGQTQSLGVVTLGTGSD
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCEEEEECCCCC
NQRYETLEGQKEKSFMLHYNFPAFSVGEVRRSSGPGRREIGHGNLAERALKLVLPKSEEF
CCCCHHCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
PYVIRVVSEILESNGSSSMASVCSGSLALMAAGVPIKGSVAGIAMGLFSDSSGKYAVLSD
CHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCHHHEEEEEEECCCCCEEEHHH
IAGLEDHFGDMDCKIAGTRKGITAFQMDLKVTGVSFDVLESVFEQAQRGRFHILDIMEKH
HCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCEEHHHHHHHH
ISKASDSLAGTAPRIIVRNIPKDRIGELIGPGGKNVRGISELTGAELYIEDDGRVTISGS
HHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCCCCCCCHHHCCCCEEEEECCCEEEEECC
NQESAEKAAKMVDGFFAEVEVGKIYEGKVKRIADFGAFVEILPGKEGLCHISKIDFKRVN
CHHHHHHHHHHHHHHHHEEEECCEECCHHHHHHHHCCCEEECCCCCCCEEEECCCHHHHH
SVKDIVKEGDIIRVKVLNVDKTGKIDLSRKDALEEI
HHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA