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The map label for this gene is truA [H]

Identifier: 116328579

GI number: 116328579

Start: 2259150

End: 2259947

Strand: Direct

Name: truA [H]

Synonym: LBL_1936

Alternate gene names: 116328579

Gene position: 2259150-2259947 (Clockwise)

Preceding gene: 116328578

Following gene: 116328580

Centisome position: 62.5

GC content: 42.86

Gene sequence:

>798_bases
ATAAACTACGCCCTCCTCGTCGAATACGACGGACTTTGCTTCAACGGTTTCCAAACTCAAAAAGACCTGCCCAGCATTCA
GGAAAATCTCGAAAAAGCCGTAAAAATTCTCCTCAAGGAAGAGATCTCGATTACGGGAGCCGGCAGAACGGACACAGGAG
TTCACGCTCGAGGGATGGTAGTGAATTTCAAGACGATCAAAACCGTACAGAATTTCAATAAGTTCCTTTTGAGTATGAAT
GCAATCACGGACTCGGGGCTTTCCATTCTGGGTATGACGGAAGTGGGCGAAAATTTCGATTCCCGCTTTTCCTGCAATTC
GAGAGAATATGAATATTTGATTCTCAATACGAAATACCCAAGGCCTATTTGGAAAAATCGAGCCTTCTGGTATCAACATA
AGATTGACGTTCCACGCTTGAAAACCGAACTTGAACTACTAAAAGGAGAACACGATTTTCGTAGTTTGGCCAAGACTGCG
TCCATGAAAGATCGTTCCACGATTCGGACAATTTTAGATGCAAAACTGGAACGAAGCTCCGAATGGGAAGGTCTCTTAAA
AATAAAGATCCGGGCGAACGGCTTTCTTCATAATATGATTCGGATTCTTACGGGTACTCTTCTGGAAATCGCAAACGGCA
AACTCAAGGTCCCGAACATTTTGGATATTCTCTCTTCCAAAGACAGAACGATTGCGGGTATCACTCTTCCTCCCTATGGA
CTTTATTTCCTAAGAGCGTATTACGATTCCAATCCTGAAATCGATTTTATGTATTCCCATCGGGACTTTCAAAATTAA

Upstream 100 bases:

>100_bases
CTGGCAAAGGATCCAAATCTAAACCCTCTGTCATTATTGATATGGCAAAGGAACTCTACGACGAGTATAATAAGCTCATC
GAGGAAATGGGAGAGGAAAA

Downstream 100 bases:

>100_bases
TCATGTCTTTTCTATTTCGAATTTTTACAAAATCTCCGATTCTCATTTTATTCGTAATCGTTCCCGGAATTGTATCCGCT
TCTCCTAAAAAACCGGGAGC

Product: pseudouridylate synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNYALLVEYDGLCFNGFQTQKDLPSIQENLEKAVKILLKEEISITGAGRTDTGVHARGMVVNFKTIKTVQNFNKFLLSMN
AITDSGLSILGMTEVGENFDSRFSCNSREYEYLILNTKYPRPIWKNRAFWYQHKIDVPRLKTELELLKGEHDFRSLAKTA
SMKDRSTIRTILDAKLERSSEWEGLLKIKIRANGFLHNMIRILTGTLLEIANGKLKVPNILDILSSKDRTIAGITLPPYG
LYFLRAYYDSNPEIDFMYSHRDFQN

Sequences:

>Translated_265_residues
MNYALLVEYDGLCFNGFQTQKDLPSIQENLEKAVKILLKEEISITGAGRTDTGVHARGMVVNFKTIKTVQNFNKFLLSMN
AITDSGLSILGMTEVGENFDSRFSCNSREYEYLILNTKYPRPIWKNRAFWYQHKIDVPRLKTELELLKGEHDFRSLAKTA
SMKDRSTIRTILDAKLERSSEWEGLLKIKIRANGFLHNMIRILTGTLLEIANGKLKVPNILDILSSKDRTIAGITLPPYG
LYFLRAYYDSNPEIDFMYSHRDFQN
>Mature_265_residues
MNYALLVEYDGLCFNGFQTQKDLPSIQENLEKAVKILLKEEISITGAGRTDTGVHARGMVVNFKTIKTVQNFNKFLLSMN
AITDSGLSILGMTEVGENFDSRFSCNSREYEYLILNTKYPRPIWKNRAFWYQHKIDVPRLKTELELLKGEHDFRSLAKTA
SMKDRSTIRTILDAKLERSSEWEGLLKIKIRANGFLHNMIRILTGTLLEIANGKLKVPNILDILSSKDRTIAGITLPPYG
LYFLRAYYDSNPEIDFMYSHRDFQN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=32.520325203252, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30489; Mature: 30489

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYALLVEYDGLCFNGFQTQKDLPSIQENLEKAVKILLKEEISITGAGRTDTGVHARGMV
CCEEEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCEECEEE
VNFKTIKTVQNFNKFLLSMNAITDSGLSILGMTEVGENFDSRFSCNSREYEYLILNTKYP
EEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCCCCCCEEEEEEECCCC
RPIWKNRAFWYQHKIDVPRLKTELELLKGEHDFRSLAKTASMKDRSTIRTILDAKLERSS
CCHHCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
EWEGLLKIKIRANGFLHNMIRILTGTLLEIANGKLKVPNILDILSSKDRTIAGITLPPYG
CCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHCCCCCEEEEEECCCHH
LYFLRAYYDSNPEIDFMYSHRDFQN
HHHHEEEECCCCCEEEEECCCCCCC
>Mature Secondary Structure
MNYALLVEYDGLCFNGFQTQKDLPSIQENLEKAVKILLKEEISITGAGRTDTGVHARGMV
CCEEEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCEECEEE
VNFKTIKTVQNFNKFLLSMNAITDSGLSILGMTEVGENFDSRFSCNSREYEYLILNTKYP
EEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCCCCCCEEEEEEECCCC
RPIWKNRAFWYQHKIDVPRLKTELELLKGEHDFRSLAKTASMKDRSTIRTILDAKLERSS
CCHHCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
EWEGLLKIKIRANGFLHNMIRILTGTLLEIANGKLKVPNILDILSSKDRTIAGITLPPYG
CCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHCCCCCEEEEEECCCHH
LYFLRAYYDSNPEIDFMYSHRDFQN
HHHHEEEECCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]