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The map label for this gene is xthA [H]

Identifier: 116328536

GI number: 116328536

Start: 2203792

End: 2204556

Strand: Direct

Name: xthA [H]

Synonym: LBL_1888

Alternate gene names: 116328536

Gene position: 2203792-2204556 (Clockwise)

Preceding gene: 116328525

Following gene: 116328539

Centisome position: 60.97

GC content: 42.88

Gene sequence:

>765_bases
ATGAAACTCATCTCTCTCAACTGCAACGGAATCAGATCCTCTCTCGGAAAAGGACTTTCTGATTATATCCGGGATAGAAA
ACCGGACTTCATTTGCTTTCAAGAAACAAAGGCGACTCAGGAACAGATTCCACCTTCTCTCTGGGAAGAAGGTGGATATA
CTCCTGTGTTTCACAGCGCGGAAAAGAAAGGATACAGTGGAGTCGCCGTTCTTTATAAAAAACCCCCGCAGAAAATTACA
ATCGGCCTCGGAGATCCATTCTTTGATAAAGAAGGCAGAAGTATCTATTTGGAGTATCCGGACTTTGCACTTTGGAACTC
TTACTTCCCTTCTGGAACAACAGGAGAAGTACGCCAGGCTGTAAAGATGAAATTTCTAGATCTTTTTCAGAAGGAGGCGG
CCAAAAGAAGAAAAAAACAGCCCAACATCCTTCTCTGTGGAGACATAAACATCGCACATACTCCCCAAGATATTCACGAC
CCGAAAGGGAACGCAAAAAGCAGCGGCTTTTTACCGGAAGAAAGAGAATGGTTGACTCAATTTTTAGAAAAGGGTTGGGT
AGATACCTTTCGTTATCTTTATCCCGAAAAACAGGAGTATTCCTGGTGGACGTTTCGAGCGGGAGCCAGAGCTAAAAACA
AAGGATGGAGAATCGACTACTTCTTTGTAACGGCCGAATTGAAAAAAAACGTAAAAAGCCAATCGATTTATAGAGACAAA
CCGCTTTCCGATCACGCTCCATTGGAACTGGAAATCAAATTATAG

Upstream 100 bases:

>100_bases
ATCAAAAAACCAGAATTTCCGAAAGAACTGTTCCTGCATCTGAAATTTACCCAAAAAACAATTGCCCTGGAAGAGAATTC
ATCCAATTTACACCTTGCCT

Downstream 100 bases:

>100_bases
ACGTTCAAGAGTAAATTCTTTCTTAAGATCTCAAAAATTGTAGTAGTTCCTACATTTTGAGGTTTTGGGACGAGTTCGAT
TTCTCTTCAGGTTTTGGACT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKLISLNCNGIRSSLGKGLSDYIRDRKPDFICFQETKATQEQIPPSLWEEGGYTPVFHSAEKKGYSGVAVLYKKPPQKIT
IGLGDPFFDKEGRSIYLEYPDFALWNSYFPSGTTGEVRQAVKMKFLDLFQKEAAKRRKKQPNILLCGDINIAHTPQDIHD
PKGNAKSSGFLPEEREWLTQFLEKGWVDTFRYLYPEKQEYSWWTFRAGARAKNKGWRIDYFFVTAELKKNVKSQSIYRDK
PLSDHAPLELEIKL

Sequences:

>Translated_254_residues
MKLISLNCNGIRSSLGKGLSDYIRDRKPDFICFQETKATQEQIPPSLWEEGGYTPVFHSAEKKGYSGVAVLYKKPPQKIT
IGLGDPFFDKEGRSIYLEYPDFALWNSYFPSGTTGEVRQAVKMKFLDLFQKEAAKRRKKQPNILLCGDINIAHTPQDIHD
PKGNAKSSGFLPEEREWLTQFLEKGWVDTFRYLYPEKQEYSWWTFRAGARAKNKGWRIDYFFVTAELKKNVKSQSIYRDK
PLSDHAPLELEIKL
>Mature_254_residues
MKLISLNCNGIRSSLGKGLSDYIRDRKPDFICFQETKATQEQIPPSLWEEGGYTPVFHSAEKKGYSGVAVLYKKPPQKIT
IGLGDPFFDKEGRSIYLEYPDFALWNSYFPSGTTGEVRQAVKMKFLDLFQKEAAKRRKKQPNILLCGDINIAHTPQDIHD
PKGNAKSSGFLPEEREWLTQFLEKGWVDTFRYLYPEKQEYSWWTFRAGARAKNKGWRIDYFFVTAELKKNVKSQSIYRDK
PLSDHAPLELEIKL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=36.4341085271318, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=36.4341085271318, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=36.4341085271318, Blast_Score=181, Evalue=6e-46,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=32.4626865671642, Blast_Score=117, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71989536, Length=255, Percent_Identity=32.9411764705882, Blast_Score=153, Evalue=7e-38,
Organism=Drosophila melanogaster, GI221330655, Length=251, Percent_Identity=37.8486055776892, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI17136678, Length=251, Percent_Identity=37.8486055776892, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29418; Mature: 29418

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISLNCNGIRSSLGKGLSDYIRDRKPDFICFQETKATQEQIPPSLWEEGGYTPVFHSA
CEEEEECCCHHHHHHCCCHHHHHHCCCCCEEEEECCCCHHHHCCHHHHHCCCCCCEEECC
EKKGYSGVAVLYKKPPQKITIGLGDPFFDKEGRSIYLEYPDFALWNSYFPSGTTGEVRQA
CCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCHHHHHH
VKMKFLDLFQKEAAKRRKKQPNILLCGDINIAHTPQDIHDPKGNAKSSGFLPEEREWLTQ
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHH
FLEKGWVDTFRYLYPEKQEYSWWTFRAGARAKNKGWRIDYFFVTAELKKNVKSQSIYRDK
HHHCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEEEEHHHHHCHHHHHHHCCC
PLSDHAPLELEIKL
CCCCCCCEEEEEEC
>Mature Secondary Structure
MKLISLNCNGIRSSLGKGLSDYIRDRKPDFICFQETKATQEQIPPSLWEEGGYTPVFHSA
CEEEEECCCHHHHHHCCCHHHHHHCCCCCEEEEECCCCHHHHCCHHHHHCCCCCCEEECC
EKKGYSGVAVLYKKPPQKITIGLGDPFFDKEGRSIYLEYPDFALWNSYFPSGTTGEVRQA
CCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCHHHHHH
VKMKFLDLFQKEAAKRRKKQPNILLCGDINIAHTPQDIHDPKGNAKSSGFLPEEREWLTQ
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHH
FLEKGWVDTFRYLYPEKQEYSWWTFRAGARAKNKGWRIDYFFVTAELKKNVKSQSIYRDK
HHHCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEEEEHHHHHCHHHHHHHCCC
PLSDHAPLELEIKL
CCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]