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The map label for this gene is wcaJ [H]

Identifier: 116328529

GI number: 116328529

Start: 2191985

End: 2193400

Strand: Reverse

Name: wcaJ [H]

Synonym: LBL_1881

Alternate gene names: 116328529

Gene position: 2193400-2191985 (Counterclockwise)

Preceding gene: 116328530

Following gene: 116328528

Centisome position: 60.68

GC content: 41.31

Gene sequence:

>1416_bases
ATGTTGAAGGAAAGAAATCAAACATTCAAACTGATCTTTACAGGTTTAGATTTTGCGAATGCTTTATTTAGCGGGATGCT
GGCGTTCGTATTCCGATTTTATTTTTTGGACGAAAACGGAACGGATCGCCGTTACGTGGACGTGGAAAGTTATATTTTTC
TTTTTTTTATGTTGGCGTTTTTTCAGGTCATTGTGTTCATCGCGATCGATCTTTATCATCCGAGAAGAGGTCTTTCTTTT
ATCGACGAATTTTTGACGATCGTAAGCGGAGTGTTTCTTAATCTTGTATTGGTTCTTGCGGTTTTATTTTTCTTTCGGGG
AGATTTAGGAAGCGAACGATTTTCCAGATACGTGGTTCTTGCGTTTGCGATCATCAACATTCTGACTTCGGGTACTCTCC
ATTATACGGCTAGGATCGTGTTGCGGGTTCTTCGTAAAAAAGGATACAATTTAAGAAGTGTTTTGGTAATAGGGATTTTT
GAAACGGCCAAACGTTTTAACGATGCGGTCATCAAACACGGAATTTACGGTTACAAAGTTTTAGGATTCGTCCAAACCAA
AGCCGTAAAACCGGTGCGAAAAGATATGAAAGTGATCGGTAAGGTCGAAAATATCTGCCGGATTCTGGAAAAAGAAAGAC
CGGACTTGGTCGTATATACTCTCGAACCTTCCGAGGGCGATTATCTTAAGGAAGTTTTGGACGCATGCGATCACGAAGGA
ATCGATCTCAAGATCGTTCCCGGATTCCAGGAATTCATCAAGGCTCGGGGAAGAGTGGAGGAGATGGAAGGACTTCCCGT
AATTTCGATCCGGAACATTCCGATCCGATTGGGTTATAATAAATTTATCAAAAGGATTTTCGATCTTATCTTTTCTGTTC
TTTTCATATTATTTTTTTCTCCCTTTTATCTTGTGATGGCTTTACTCGTAAAATTGACTTCACGAGGTCCCGTGTTTTAC
TATCAAGAACGAGTGGGATTGGACAATAGAAAGTTTAAGATGATCAAATTTCGAACCATGGTCGTTCAGGCCAAGAGTCA
ATCCGAAACAACTTGGACGGTTCAAAACGATCCGAGAGTCACGAGCGTTGGAAAAATTCTCCGAAAACTTTCCTTAGACG
AAACTCCCCAGTTTTTCAACGTCCTTTTCGGAGATATGTCGGTTGTGGGGCCGAGACCGGAACGTCCTCATTTTGTGGAA
AAGTTCAAGAATGATCACAGACATTATATGAGAAGACATGCCGTTAAAGCCGGAATCACGGGGCTTGCTCAGATAAAAGG
ACTTCGGGGAGATACTTCGATCGACGATCGGATAGCTGCCGACATCTATTACATCGAAAACTGGTCTCTTTGGCTCGATC
TCAAGATTATTCTACTGACTCCTTTTAAAGGAATCATGGACAAAAACGCATACTAA

Upstream 100 bases:

>100_bases
CAGGATTCTTCGGAACTGACAAGAGTTCGTTTGTTTAGATTCCGAAAACGACCTTTCCAAGCGGGCGACAGCGCGGGAAT
TTCTTCGGAGAACCCTTGAT

Downstream 100 bases:

>100_bases
AACAACTTTGGACGCAAGGGGAGAATCATGAACTTGAACGAAGATTCTTTGCGAAAAATCGCCGAACTCTCGCGTTTGAA
CATCCGTCCTAAGGAAAAGG

Product: bifunctional glycosyl transferase/glycosyl epimerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLKERNQTFKLIFTGLDFANALFSGMLAFVFRFYFLDENGTDRRYVDVESYIFLFFMLAFFQVIVFIAIDLYHPRRGLSF
IDEFLTIVSGVFLNLVLVLAVLFFFRGDLGSERFSRYVVLAFAIINILTSGTLHYTARIVLRVLRKKGYNLRSVLVIGIF
ETAKRFNDAVIKHGIYGYKVLGFVQTKAVKPVRKDMKVIGKVENICRILEKERPDLVVYTLEPSEGDYLKEVLDACDHEG
IDLKIVPGFQEFIKARGRVEEMEGLPVISIRNIPIRLGYNKFIKRIFDLIFSVLFILFFSPFYLVMALLVKLTSRGPVFY
YQERVGLDNRKFKMIKFRTMVVQAKSQSETTWTVQNDPRVTSVGKILRKLSLDETPQFFNVLFGDMSVVGPRPERPHFVE
KFKNDHRHYMRRHAVKAGITGLAQIKGLRGDTSIDDRIAADIYYIENWSLWLDLKIILLTPFKGIMDKNAY

Sequences:

>Translated_471_residues
MLKERNQTFKLIFTGLDFANALFSGMLAFVFRFYFLDENGTDRRYVDVESYIFLFFMLAFFQVIVFIAIDLYHPRRGLSF
IDEFLTIVSGVFLNLVLVLAVLFFFRGDLGSERFSRYVVLAFAIINILTSGTLHYTARIVLRVLRKKGYNLRSVLVIGIF
ETAKRFNDAVIKHGIYGYKVLGFVQTKAVKPVRKDMKVIGKVENICRILEKERPDLVVYTLEPSEGDYLKEVLDACDHEG
IDLKIVPGFQEFIKARGRVEEMEGLPVISIRNIPIRLGYNKFIKRIFDLIFSVLFILFFSPFYLVMALLVKLTSRGPVFY
YQERVGLDNRKFKMIKFRTMVVQAKSQSETTWTVQNDPRVTSVGKILRKLSLDETPQFFNVLFGDMSVVGPRPERPHFVE
KFKNDHRHYMRRHAVKAGITGLAQIKGLRGDTSIDDRIAADIYYIENWSLWLDLKIILLTPFKGIMDKNAY
>Mature_471_residues
MLKERNQTFKLIFTGLDFANALFSGMLAFVFRFYFLDENGTDRRYVDVESYIFLFFMLAFFQVIVFIAIDLYHPRRGLSF
IDEFLTIVSGVFLNLVLVLAVLFFFRGDLGSERFSRYVVLAFAIINILTSGTLHYTARIVLRVLRKKGYNLRSVLVIGIF
ETAKRFNDAVIKHGIYGYKVLGFVQTKAVKPVRKDMKVIGKVENICRILEKERPDLVVYTLEPSEGDYLKEVLDACDHEG
IDLKIVPGFQEFIKARGRVEEMEGLPVISIRNIPIRLGYNKFIKRIFDLIFSVLFILFFSPFYLVMALLVKLTSRGPVFY
YQERVGLDNRKFKMIKFRTMVVQAKSQSETTWTVQNDPRVTSVGKILRKLSLDETPQFFNVLFGDMSVVGPRPERPHFVE
KFKNDHRHYMRRHAVKAGITGLAQIKGLRGDTSIDDRIAADIYYIENWSLWLDLKIILLTPFKGIMDKNAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=430, Percent_Identity=33.0232558139535, Blast_Score=229, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 54658; Mature: 54658

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKERNQTFKLIFTGLDFANALFSGMLAFVFRFYFLDENGTDRRYVDVESYIFLFFMLAF
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
FQVIVFIAIDLYHPRRGLSFIDEFLTIVSGVFLNLVLVLAVLFFFRGDLGSERFSRYVVL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AFAIINILTSGTLHYTARIVLRVLRKKGYNLRSVLVIGIFETAKRFNDAVIKHGIYGYKV
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEHHEEEHHHHHHHHHHHHHHHCCCCHHHH
LGFVQTKAVKPVRKDMKVIGKVENICRILEKERPDLVVYTLEPSEGDYLKEVLDACDHEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCC
IDLKIVPGFQEFIKARGRVEEMEGLPVISIRNIPIRLGYNKFIKRIFDLIFSVLFILFFS
CEEEEECCHHHHHHHCCCHHHHCCCCEEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHH
PFYLVMALLVKLTSRGPVFYYQERVGLDNRKFKMIKFRTMVVQAKSQSETTWTVQNDPRV
HHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCEEHEEEHHHHEEECCCCCCEEEECCCCCH
TSVGKILRKLSLDETPQFFNVLFGDMSVVGPRPERPHFVEKFKNDHRHYMRRHAVKAGIT
HHHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
GLAQIKGLRGDTSIDDRIAADIYYIENWSLWLDLKIILLTPFKGIMDKNAY
HHHHHCCCCCCCCCCHHHHEEEEEEECCEEEEEEEEEEECCCHHHHCCCCC
>Mature Secondary Structure
MLKERNQTFKLIFTGLDFANALFSGMLAFVFRFYFLDENGTDRRYVDVESYIFLFFMLAF
CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
FQVIVFIAIDLYHPRRGLSFIDEFLTIVSGVFLNLVLVLAVLFFFRGDLGSERFSRYVVL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AFAIINILTSGTLHYTARIVLRVLRKKGYNLRSVLVIGIFETAKRFNDAVIKHGIYGYKV
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEHHEEEHHHHHHHHHHHHHHHCCCCHHHH
LGFVQTKAVKPVRKDMKVIGKVENICRILEKERPDLVVYTLEPSEGDYLKEVLDACDHEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCC
IDLKIVPGFQEFIKARGRVEEMEGLPVISIRNIPIRLGYNKFIKRIFDLIFSVLFILFFS
CEEEEECCHHHHHHHCCCHHHHCCCCEEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHH
PFYLVMALLVKLTSRGPVFYYQERVGLDNRKFKMIKFRTMVVQAKSQSETTWTVQNDPRV
HHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCEEHEEEHHHHEEECCCCCCEEEECCCCCH
TSVGKILRKLSLDETPQFFNVLFGDMSVVGPRPERPHFVEKFKNDHRHYMRRHAVKAGIT
HHHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
GLAQIKGLRGDTSIDDRIAADIYYIENWSLWLDLKIILLTPFKGIMDKNAY
HHHHHCCCCCCCCCCHHHHEEEEEEECCEEEEEEEEEEECCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]