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The map label for this gene is folD

Identifier: 116328520

GI number: 116328520

Start: 2179486

End: 2180340

Strand: Direct

Name: folD

Synonym: LBL_1870

Alternate gene names: 116328520

Gene position: 2179486-2180340 (Clockwise)

Preceding gene: 116328517

Following gene: 116328521

Centisome position: 60.3

GC content: 46.9

Gene sequence:

>855_bases
ATGGATCCGATTCTCTTAGATGGAAAAAAACTCTCTGAAAAAATCAGAAATGAAATTCGCCGCGAAATCGAGGAACGAAA
AACAAAAAATCTTAGGATTCCAAAACTTGCGACGATCCTAGTCGGAAACAACCCCGCTTCCGAAACTTATGTTTCCATGA
AGATCAAAGCCTGCCATGGCGTGGGGATGGGTTCGGAAATGATCCGATTGGGAGAACAGACGACGACGGAAGAATTACTC
TCGGTCATAGACAAACTCAACGCCGATCCGAACGTCGACGGAATCCTACTTCAACATCCTTCTCCCTCTCAAATCGACGA
ACGAGCCGCCTTCGATCGCATCTCTTTCCGCAAAGACGTGGATGGAGTCACTACTCTTTCTTTCGGAAAACTCTCCATGG
GAGTGGAAACCTATCTTCCTTGTACTCCCTACGGTATCGTTCTTTTACTGAAAGAACACGGTATCAACGTTTCGGGCAAA
AACGCTGTCGTAGTTGGACGTTCTCCGATTTTGGGAAAACCAATGGCGATGCTTCTCACGGAAATGAACGCAACCGTCAC
ACTTTGTCATTCCAAAACCCAAAATCTTCCGGAGATCGTTCGTCTGGCGGATATCGTCGTCGGGGCGGTAGGCAAACCGG
AATTCATCAAGGCTGATTGGATTTCCAAAGGTGCAGTTCTTTTGGATGCGGGTTACAACCCGGGAAACGTGGGAGACATT
GAAATTTCCAAGGCAAAAAATCATTCCTCTTTTTATACTCCGGTTCCGGGCGGAGTCGGCCCGATGACAATTGCAGTACT
TCTTCTACAGACCCTTTATTCCTCAAAAGAACACTTTACACCACCGGTTCAGTGA

Upstream 100 bases:

>100_bases
CGATCGGTATTTCAGACATAGACTCATTTTACGCTTTCGGTTTCACCGGAAAGGATATTTTTTACCTGACAGAGAAGATA
TACATTAAATCCTGAGAAGT

Downstream 100 bases:

>100_bases
AAACGATGTTTCCAGATGGCGATCAGTTTTGACGAATGTTTTCAAACTTATCCGGAGCTTCACAGCCCTACCAATCTCGA
CGAATTTTGGTCCGAGGCAA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MDPILLDGKKLSEKIRNEIRREIEERKTKNLRIPKLATILVGNNPASETYVSMKIKACHGVGMGSEMIRLGEQTTTEELL
SVIDKLNADPNVDGILLQHPSPSQIDERAAFDRISFRKDVDGVTTLSFGKLSMGVETYLPCTPYGIVLLLKEHGINVSGK
NAVVVGRSPILGKPMAMLLTEMNATVTLCHSKTQNLPEIVRLADIVVGAVGKPEFIKADWISKGAVLLDAGYNPGNVGDI
EISKAKNHSSFYTPVPGGVGPMTIAVLLLQTLYSSKEHFTPPVQ

Sequences:

>Translated_284_residues
MDPILLDGKKLSEKIRNEIRREIEERKTKNLRIPKLATILVGNNPASETYVSMKIKACHGVGMGSEMIRLGEQTTTEELL
SVIDKLNADPNVDGILLQHPSPSQIDERAAFDRISFRKDVDGVTTLSFGKLSMGVETYLPCTPYGIVLLLKEHGINVSGK
NAVVVGRSPILGKPMAMLLTEMNATVTLCHSKTQNLPEIVRLADIVVGAVGKPEFIKADWISKGAVLLDAGYNPGNVGDI
EISKAKNHSSFYTPVPGGVGPMTIAVLLLQTLYSSKEHFTPPVQ
>Mature_284_residues
MDPILLDGKKLSEKIRNEIRREIEERKTKNLRIPKLATILVGNNPASETYVSMKIKACHGVGMGSEMIRLGEQTTTEELL
SVIDKLNADPNVDGILLQHPSPSQIDERAAFDRISFRKDVDGVTTLSFGKLSMGVETYLPCTPYGIVLLLKEHGINVSGK
NAVVVGRSPILGKPMAMLLTEMNATVTLCHSKTQNLPEIVRLADIVVGAVGKPEFIKADWISKGAVLLDAGYNPGNVGDI
EISKAKNHSSFYTPVPGGVGPMTIAVLLLQTLYSSKEHFTPPVQ

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=295, Percent_Identity=42.0338983050847, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=40.3389830508475, Blast_Score=207, Evalue=7e-54,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=41.0169491525424, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI36796743, Length=237, Percent_Identity=28.2700421940928, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1786741, Length=281, Percent_Identity=44.8398576512456, Blast_Score=239, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17568735, Length=288, Percent_Identity=37.8472222222222, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=42.7083333333333, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=40.5405405405405, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322933, Length=308, Percent_Identity=25.6493506493506, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=40.4761904761905, Blast_Score=215, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=40.4761904761905, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=41.6083916083916, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=41.6083916083916, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=41.6083916083916, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=41.6083916083916, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_LEPBJ (Q04SB2)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800966.1
- ProteinModelPortal:   Q04SB2
- SMR:   Q04SB2
- STRING:   Q04SB2
- GeneID:   4411042
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1651
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   GPMTINT
- PhylomeDB:   Q04SB2
- ProtClustDB:   PRK14177
- BioCyc:   LBOR355277:LBJ_1651-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30854; Mature: 30854

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPILLDGKKLSEKIRNEIRREIEERKTKNLRIPKLATILVGNNPASETYVSMKIKACHG
CCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEEEEECC
VGMGSEMIRLGEQTTTEELLSVIDKLNADPNVDGILLQHPSPSQIDERAAFDRISFRKDV
CCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
DGVTTLSFGKLSMGVETYLPCTPYGIVLLLKEHGINVSGKNAVVVGRSPILGKPMAMLLT
CCCEEEECCHHHCCHHHCCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHH
EMNATVTLCHSKTQNLPEIVRLADIVVGAVGKPEFIKADWISKGAVLLDAGYNPGNVGDI
HCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHCCCCEEEEECCCCCCCCCEE
EISKAKNHSSFYTPVPGGVGPMTIAVLLLQTLYSSKEHFTPPVQ
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure
MDPILLDGKKLSEKIRNEIRREIEERKTKNLRIPKLATILVGNNPASETYVSMKIKACHG
CCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEEEEECC
VGMGSEMIRLGEQTTTEELLSVIDKLNADPNVDGILLQHPSPSQIDERAAFDRISFRKDV
CCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
DGVTTLSFGKLSMGVETYLPCTPYGIVLLLKEHGINVSGKNAVVVGRSPILGKPMAMLLT
CCCEEEECCHHHCCHHHCCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHH
EMNATVTLCHSKTQNLPEIVRLADIVVGAVGKPEFIKADWISKGAVLLDAGYNPGNVGDI
HCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHCCCCEEEEECCCCCCCCCEE
EISKAKNHSSFYTPVPGGVGPMTIAVLLLQTLYSSKEHFTPPVQ
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA