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The map label for this gene is fliN-1 [H]

Identifier: 116328500

GI number: 116328500

Start: 2151600

End: 2152661

Strand: Reverse

Name: fliN-1 [H]

Synonym: LBL_1848

Alternate gene names: 116328500

Gene position: 2152661-2151600 (Counterclockwise)

Preceding gene: 116328501

Following gene: 116328499

Centisome position: 59.56

GC content: 46.14

Gene sequence:

>1062_bases
ATGGGTGAAGGTTCCCTTTCACAGGAAGAGATTGACGCATTACTCGCCGGAGCAAGTGATTCATTTGATTCGGGTGTTGT
GGCCTCTAGTGCCACACAAAAAGAAGTTCCAGGAATGTCTCCTGTGGATCGGGATATTCTGTCGGATCTTCTTTCTTCCT
GTTTTCAAGTCGCTGGGAACACATTAGGCGCGGTTCTTTCCCGTTCTTCTGCATTTTTAAACCCGAACGTGGAAACCAAG
TCTCGAAAGGATGTGGAATCTGAACTTAAATCGGGATCTTTTCTTTTATGTTCGACTCTATCTGGAAGTGTGAACGGGAG
AATCGTTCTAGCGATGTCTGCAGAAAATGCGGTTAAAATCGCAAATTCTATGATGGGTGGCTTTGAATCGGGAGAGCTGG
ATGAAGCTCAAATACAGACACTTCGAGATTCCCTTACTCCTGTAATGGGAGCCTTAATTTCTCAAATCAGTACGAAAACT
GGGGGTGGGGTGAATGGCTCTCCCTCTGAAACTAGAAATGTAACTTCTCCTGCCGCACTAGTTCTACCAGACGGAGAAAC
TATGGTGCGTGTTTTTTTTAACCTTACTATCGAAAATATTCCTTCCTTTCGAGTTCAATTCCTGCTTTCTCTATCCACTG
CCTCGGATTTGTTGAATCTTTATCGCCGTTCCGGAAGTGTCGGCGGTGATATGAGCAATATGGGAATGGGAGGGATGGGA
ATGGGAGCCTCCCCAGGTTCCATGTCGGTTAAATCGGTCGCATTCCCGAATTTAGGAACCGCGAGTGGCGCATCAAACAC
TCCGAACCTAAATCTTCTCATGGACGTTCAGATGAGTGTAACGGTGGAGTTAGGAAGAACCAAGATGTATATCAAAGATA
TCTTAGGTTTGGGCGAGGGTTCCATCATAGAGTTGGATAAACTCGCGGGCGAGCCCGTGGATCTTTTGGTAAACGGAAAA
CTGATTGCAAAAGGCGAGGTTGTCGTTATCGATGAAAACTTTGGTGTGCGGGTAACGGATATAGTAAGTCCTATCGATCG
GATCAAGCCGGAGGGTAAGTGA

Upstream 100 bases:

>100_bases
GCCCGAAAATTAGGTGGAATCAGGTTGGGAAGAAAAAAAGGGGTGACAAAAATCCTTTTCTTATGTCCCATAAACTAAAA
TTCCCGGGAGAGTTCCCAAA

Downstream 100 bases:

>100_bases
GATTTTTAGGCGCTCTTCGAATGGGCGGAGGGAATGTCCCTTTTATCTTTGCTGTTTTAGTTATATTCAATTTTACTGTT
GTTTCTTTGAGCGCTCAATC

Product: endoflagellar motor switch protein

Products: NA

Alternate protein names: CheY-P phosphatase FliY [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MGEGSLSQEEIDALLAGASDSFDSGVVASSATQKEVPGMSPVDRDILSDLLSSCFQVAGNTLGAVLSRSSAFLNPNVETK
SRKDVESELKSGSFLLCSTLSGSVNGRIVLAMSAENAVKIANSMMGGFESGELDEAQIQTLRDSLTPVMGALISQISTKT
GGGVNGSPSETRNVTSPAALVLPDGETMVRVFFNLTIENIPSFRVQFLLSLSTASDLLNLYRRSGSVGGDMSNMGMGGMG
MGASPGSMSVKSVAFPNLGTASGASNTPNLNLLMDVQMSVTVELGRTKMYIKDILGLGEGSIIELDKLAGEPVDLLVNGK
LIAKGEVVVIDENFGVRVTDIVSPIDRIKPEGK

Sequences:

>Translated_353_residues
MGEGSLSQEEIDALLAGASDSFDSGVVASSATQKEVPGMSPVDRDILSDLLSSCFQVAGNTLGAVLSRSSAFLNPNVETK
SRKDVESELKSGSFLLCSTLSGSVNGRIVLAMSAENAVKIANSMMGGFESGELDEAQIQTLRDSLTPVMGALISQISTKT
GGGVNGSPSETRNVTSPAALVLPDGETMVRVFFNLTIENIPSFRVQFLLSLSTASDLLNLYRRSGSVGGDMSNMGMGGMG
MGASPGSMSVKSVAFPNLGTASGASNTPNLNLLMDVQMSVTVELGRTKMYIKDILGLGEGSIIELDKLAGEPVDLLVNGK
LIAKGEVVVIDENFGVRVTDIVSPIDRIKPEGK
>Mature_352_residues
GEGSLSQEEIDALLAGASDSFDSGVVASSATQKEVPGMSPVDRDILSDLLSSCFQVAGNTLGAVLSRSSAFLNPNVETKS
RKDVESELKSGSFLLCSTLSGSVNGRIVLAMSAENAVKIANSMMGGFESGELDEAQIQTLRDSLTPVMGALISQISTKTG
GGVNGSPSETRNVTSPAALVLPDGETMVRVFFNLTIENIPSFRVQFLLSLSTASDLLNLYRRSGSVGGDMSNMGMGGMGM
GASPGSMSVKSVAFPNLGTASGASNTPNLNLLMDVQMSVTVELGRTKMYIKDILGLGEGSIIELDKLAGEPVDLLVNGKL
IAKGEVVVIDENFGVRVTDIVSPIDRIKPEGK

Specific function: Component of the flagellar switch. Binds CheY-P and increases its hydrolysis rate in vitro. May function constitutively to remove CheY-P around the flagellar switch to maintain an optimal level of CheY-P whereas CheC may function after addition of an attr

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=The switch is localized at the base of the flagellar motor, in or near the C-ring, attached to the MS-ring (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=121, Percent_Identity=42.1487603305785, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007597
- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF04509 CheC; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36738; Mature: 36607

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEGSLSQEEIDALLAGASDSFDSGVVASSATQKEVPGMSPVDRDILSDLLSSCFQVAGN
CCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCH
TLGAVLSRSSAFLNPNVETKSRKDVESELKSGSFLLCSTLSGSVNGRIVLAMSAENAVKI
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCHHHH
ANSMMGGFESGELDEAQIQTLRDSLTPVMGALISQISTKTGGGVNGSPSETRNVTSPAAL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
VLPDGETMVRVFFNLTIENIPSFRVQFLLSLSTASDLLNLYRRSGSVGGDMSNMGMGGMG
EECCCCCEEEHEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
MGASPGSMSVKSVAFPNLGTASGASNTPNLNLLMDVQMSVTVELGRTKMYIKDILGLGEG
CCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHCCCCC
SIIELDKLAGEPVDLLVNGKLIAKGEVVVIDENFGVRVTDIVSPIDRIKPEGK
CEEEHHHHCCCCEEEEECCEEEECCEEEEEECCCCEEHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GEGSLSQEEIDALLAGASDSFDSGVVASSATQKEVPGMSPVDRDILSDLLSSCFQVAGN
CCCCCCHHHHHHHHHCCCCCCCCCCEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCH
TLGAVLSRSSAFLNPNVETKSRKDVESELKSGSFLLCSTLSGSVNGRIVLAMSAENAVKI
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCHHHH
ANSMMGGFESGELDEAQIQTLRDSLTPVMGALISQISTKTGGGVNGSPSETRNVTSPAAL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
VLPDGETMVRVFFNLTIENIPSFRVQFLLSLSTASDLLNLYRRSGSVGGDMSNMGMGGMG
EECCCCCEEEHEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
MGASPGSMSVKSVAFPNLGTASGASNTPNLNLLMDVQMSVTVELGRTKMYIKDILGLGEG
CCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHCCCCC
SIIELDKLAGEPVDLLVNGKLIAKGEVVVIDENFGVRVTDIVSPIDRIKPEGK
CEEEHHHHCCCCEEEEECCEEEECCEEEEEECCCCEEHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1447979; 9384377; 1898932 [H]