Click here to switch to the map view.

The map label for this gene is ntrX [H]

Identifier: 116328329

GI number: 116328329

Start: 1945032

End: 1946372

Strand: Reverse

Name: ntrX [H]

Synonym: LBL_1666

Alternate gene names: 116328329

Gene position: 1946372-1945032 (Counterclockwise)

Preceding gene: 116328330

Following gene: 116328328

Centisome position: 53.85

GC content: 41.98

Gene sequence:

>1341_bases
ATGAAAATTTTTATCGTCGATGACGAACCCGAAATTAGAAAATCTCTGAGGGAAATCTTAGAAGACGAAAATTTCGAAGT
GGAAACTTTTTCCACAGGCAAAACCTTACTCAAACAGCTCAAAAATGAAAGACCTTCTTTGATTCTCTTGGATGTATGGT
TGGGCAAGGAAGACGGAATCGTCGTTTTGGATGAATGCAAAAAACTTTATCCGACTCTTCCTGTGCTTATGATTTCCGGA
CATGGAACGATCGAAATCGCAGTCTCGGCCACCAAAAAAGGAGCCGTTGATTTTTTGGAAAAACCCCTTTCCATTGAAAA
GGTTCTCCAGGCGATCGAAAATGTAATCAAACCGGAGGAAAAATATTCCTCTATTAAATTAGAATATGATGAAATTCTGG
GAAACTCTCCCGCGATTCAAAAAGTAAAGTTTGCGATTGCTCAGGCCGCTTCTACAAACGCTCGAGTTTTTATATACGGA
GAGAACGGAACCGGAAAGGAACTAGTAGCAAGAACCATATTCAAAAATTCTAAACGAAAAGACTTACCTTTTGTCGAAAT
GAACTGTGCCGCAATTCCGGAAGAGCTTATCGAATCCGAATTGTTCGGTTTTGCAAAAGGGGCTTTTACGGGGGCGACCG
ATTCCAGGATCGGAAAATTCGAAGCTGCCAACGGAGGAACCCTGTTTCTCGACGAAATTTGCGACATGTCCTTGTCGACG
CAGGCAAAGGTTTTACGAATTCTCCAGGAACAAAGATTTGAAAAATTGGGAAGCACGGAGACGATCACGGTGGATGTTAG
GATTATTGCCGCGACCAACATCCCGGTGGAAGAGGCGATTCGAGAAGGAAAGTTTAGAGAAGACTTATATTATCGATTGA
ACGTGATTCCGATTACAATTCCACCTCTTCGGGAAAGAACCTCCGATATCCCGTTGTTAGTGGACTATTACATATCTAAA
ACCTTGGAAGAAAACAACCTACCCCCGAAAAAAATCGAATCCGAGGCGATTTCCATTCTTCAAAATCATTTTTGGCCGGG
AAATATTCGGGAATTGAAAAATGTCATGGAAAGGCTTTGTATCATGACGGTGAGCCCCACGATTGCCGCAAACGACGCAA
GAGATGCGCTGAAAGGTTTCAAAACGGCAAATGAAATGGTGGAGTTAGGAGATTTTCGAAAAGCCAAGGAAGAATTTGAA
AGACAGTATATTATAAAGACGTTACAGACTAACGAAGGCAACGTGACTAGAACCTCCCGAGTTCTCGGAATAGAACGTTC
TCATTTATACAGAAAGATGAAGTCGCTTAACATTTCTTCGGATCAGTACACGGATGGATAA

Upstream 100 bases:

>100_bases
TCAAAAAAGTGTGATTGACCACAATGGTCATATTTCTGTAGATTCTTCTCGTATGGGTGGGTGTCGTTTTCAAATTGAAC
TTCCCGTATCGTAACCGATC

Downstream 100 bases:

>100_bases
AAAGATTCTCTCCGAACTCGGAAGAATTCTGGGGGATCTCCGTTTTTTGATTCAAAAAAAGCAGATTCCGGTCCTACGAT
TTTCGGGAGAACAACCTCCG

Product: response regulator of a two component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKIFIVDDEPEIRKSLREILEDENFEVETFSTGKTLLKQLKNERPSLILLDVWLGKEDGIVVLDECKKLYPTLPVLMISG
HGTIEIAVSATKKGAVDFLEKPLSIEKVLQAIENVIKPEEKYSSIKLEYDEILGNSPAIQKVKFAIAQAASTNARVFIYG
ENGTGKELVARTIFKNSKRKDLPFVEMNCAAIPEELIESELFGFAKGAFTGATDSRIGKFEAANGGTLFLDEICDMSLST
QAKVLRILQEQRFEKLGSTETITVDVRIIAATNIPVEEAIREGKFREDLYYRLNVIPITIPPLRERTSDIPLLVDYYISK
TLEENNLPPKKIESEAISILQNHFWPGNIRELKNVMERLCIMTVSPTIAANDARDALKGFKTANEMVELGDFRKAKEEFE
RQYIIKTLQTNEGNVTRTSRVLGIERSHLYRKMKSLNISSDQYTDG

Sequences:

>Translated_446_residues
MKIFIVDDEPEIRKSLREILEDENFEVETFSTGKTLLKQLKNERPSLILLDVWLGKEDGIVVLDECKKLYPTLPVLMISG
HGTIEIAVSATKKGAVDFLEKPLSIEKVLQAIENVIKPEEKYSSIKLEYDEILGNSPAIQKVKFAIAQAASTNARVFIYG
ENGTGKELVARTIFKNSKRKDLPFVEMNCAAIPEELIESELFGFAKGAFTGATDSRIGKFEAANGGTLFLDEICDMSLST
QAKVLRILQEQRFEKLGSTETITVDVRIIAATNIPVEEAIREGKFREDLYYRLNVIPITIPPLRERTSDIPLLVDYYISK
TLEENNLPPKKIESEAISILQNHFWPGNIRELKNVMERLCIMTVSPTIAANDARDALKGFKTANEMVELGDFRKAKEEFE
RQYIIKTLQTNEGNVTRTSRVLGIERSHLYRKMKSLNISSDQYTDG
>Mature_446_residues
MKIFIVDDEPEIRKSLREILEDENFEVETFSTGKTLLKQLKNERPSLILLDVWLGKEDGIVVLDECKKLYPTLPVLMISG
HGTIEIAVSATKKGAVDFLEKPLSIEKVLQAIENVIKPEEKYSSIKLEYDEILGNSPAIQKVKFAIAQAASTNARVFIYG
ENGTGKELVARTIFKNSKRKDLPFVEMNCAAIPEELIESELFGFAKGAFTGATDSRIGKFEAANGGTLFLDEICDMSLST
QAKVLRILQEQRFEKLGSTETITVDVRIIAATNIPVEEAIREGKFREDLYYRLNVIPITIPPLRERTSDIPLLVDYYISK
TLEENNLPPKKIESEAISILQNHFWPGNIRELKNVMERLCIMTVSPTIAANDARDALKGFKTANEMVELGDFRKAKEEFE
RQYIIKTLQTNEGNVTRTSRVLGIERSHLYRKMKSLNISSDQYTDG

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=41.8141592920354, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1788905, Length=443, Percent_Identity=38.8261851015801, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI1790299, Length=463, Percent_Identity=36.2850971922246, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1790437, Length=439, Percent_Identity=38.7243735763098, Blast_Score=314, Evalue=8e-87,
Organism=Escherichia coli, GI87082117, Length=361, Percent_Identity=39.8891966759003, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789087, Length=324, Percent_Identity=42.9012345679012, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=43.7106918238994, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI87082152, Length=318, Percent_Identity=38.9937106918239, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=37.9310344827586, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI87081872, Length=316, Percent_Identity=36.7088607594937, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1787583, Length=313, Percent_Identity=33.2268370607029, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081858, Length=305, Percent_Identity=30.4918032786885, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1789828, Length=262, Percent_Identity=33.587786259542, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790860, Length=146, Percent_Identity=28.0821917808219, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50364; Mature: 50364

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFIVDDEPEIRKSLREILEDENFEVETFSTGKTLLKQLKNERPSLILLDVWLGKEDGI
CEEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCCE
VVLDECKKLYPTLPVLMISGHGTIEIAVSATKKGAVDFLEKPLSIEKVLQAIENVIKPEE
EEHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHCCCH
KYSSIKLEYDEILGNSPAIQKVKFAIAQAASTNARVFIYGENGTGKELVARTIFKNSKRK
HHCEEEEEHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCC
DLPFVEMNCAAIPEELIESELFGFAKGAFTGATDSRIGKFEAANGGTLFLDEICDMSLST
CCCEEECCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEHHHHCCCCCCH
QAKVLRILQEQRFEKLGSTETITVDVRIIAATNIPVEEAIREGKFREDLYYRLNVIPITI
HHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCHHHHHHCCCCHHCEEEEEEEEEEEC
PPLRERTSDIPLLVDYYISKTLEENNLPPKKIESEAISILQNHFWPGNIRELKNVMERLC
CCHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IMTVSPTIAANDARDALKGFKTANEMVELGDFRKAKEEFERQYIIKTLQTNEGNVTRTSR
HHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHH
VLGIERSHLYRKMKSLNISSDQYTDG
HHCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIFIVDDEPEIRKSLREILEDENFEVETFSTGKTLLKQLKNERPSLILLDVWLGKEDGI
CEEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEECCCCCE
VVLDECKKLYPTLPVLMISGHGTIEIAVSATKKGAVDFLEKPLSIEKVLQAIENVIKPEE
EEHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHCCCH
KYSSIKLEYDEILGNSPAIQKVKFAIAQAASTNARVFIYGENGTGKELVARTIFKNSKRK
HHCEEEEEHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCC
DLPFVEMNCAAIPEELIESELFGFAKGAFTGATDSRIGKFEAANGGTLFLDEICDMSLST
CCCEEECCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEHHHHCCCCCCH
QAKVLRILQEQRFEKLGSTETITVDVRIIAATNIPVEEAIREGKFREDLYYRLNVIPITI
HHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCHHHHHHCCCCHHCEEEEEEEEEEEC
PPLRERTSDIPLLVDYYISKTLEENNLPPKKIESEAISILQNHFWPGNIRELKNVMERLC
CCHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IMTVSPTIAANDARDALKGFKTANEMVELGDFRKAKEEFERQYIIKTLQTNEGNVTRTSR
HHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHH
VLGIERSHLYRKMKSLNISSDQYTDG
HHCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]