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The map label for this gene is priA [H]

Identifier: 116328327

GI number: 116328327

Start: 1942254

End: 1944197

Strand: Reverse

Name: priA [H]

Synonym: LBL_1664

Alternate gene names: 116328327

Gene position: 1944197-1942254 (Counterclockwise)

Preceding gene: 116328328

Following gene: 116328326

Centisome position: 53.79

GC content: 43.16

Gene sequence:

>1944_bases
TTGATTTATTACGCCGAAGTTGCGTTTGATCTTCCGGTCGAAGAAGATACCTTCACCTACGAAGTTCCGCAGAACACCCC
AATCGGAGTCCGCGTTTTAGCAAAACTTAGAAACAGGGAAGAAGAAGGAATTATCGTATCGATTCACCAAAACGAACCGA
ATTATAAGGTTTTACCGGTGGTAAAAATTATAGATAAAGCCCCTATTGTCCTCGGGGAACAAATTGATCTTGCGTATTGG
ATGAAGGATCAATACATCGCTTCTCTCGGAGAGTGTATCTACAAGATGATTCCCGCGGGAAGAAGACAGGTTAAACTTGA
AGTATTTCCTTCGGATTCAGAAGGTAAACCCGTAACGTTAAACGAAGAACAGGAAGTCGCAACTCAAAATATACTTTCCA
CCTTCGGGCAAACTGCGGTTCATCTTCTATTCGGAATCACAGGTTCGGGAAAAACGGAAGTGTATATCCATCTGATTCAA
AAAGCGCTTGAAACTCCGAATCGTTCCGTGATTCTACTCGTGCCTGAAATCAGTTTAACGTTTCATATTATCCGGAAGTT
GGAGCTTATCTTCCCGGGACAACTAGCTGTACTTCATTCCGCACTCAAGATCTCCGAGAAGTTCAAGGCATACAACGAAC
TTTTAAGCGGTAAAAAAAGAGTCGCGGTCGGAACGAGAAGCGCAGTCTTTGCTCCGGTTTCAAACCTCGGCCTTGTCATC
ATCGACGAAGATCATGATTCTTCGTTTAAGGAACATTCAAGTCCAAGATATCACGCACGCCAGGTTGCGATGCAGAGATG
TAAAACCAACAACGCAGTTCTTGTGATGGGAACCGCGACCCCCTCTTTGGAAATCTATCATCTCGCCAAGGAAAAAAGAA
TTTATCTGCATACTCTCACCAAAAGACCGGAGGGAGTTTTACCCCCAACGGTGCGTATCGTGGAAAATAAAAAGGAGTCT
AACGTTATCAGTTCCGAACTTTCTTTCGAGATCAAACGAAGACTGGATAAAAAAGAACAGGTAATTCTTCTTTTGAACAG
GAGGGGATATAGTCCTTTAATCCACTCTTCTTCGACTTCTTCCTATATTCCTTGTCCAAATTGTACTACAAATCTTTGTT
ATCATAAAAAAGGGACTGCGGTTTGTCACCTTTGCGGACATACCGAAAATCTGGATTCCTTGGAAAAAAGAATGGGGGAG
CGATTTACACTGAAAGGAACGGGAACCCAGAAATTGGAAGAGAATCTTTTAGAAGCGTTTCCCCAAGCGAGAGTGGAACG
TTTGGACCAGGATTCCATCCAAGACAGGAGTTTGTTAAACGAAGTGATCGCCCGATTACTTGGGGGAGAAATCGATATTC
TCACCGGGACACAGATGATTGCAAAAGGGCTCGACGCTTCGCAAGTGACTCTAGTGGGGGTTTTGAACGCAGGGATCGGT
CTTGGACTTCCGGACTTTAGGGCCAACGAAAGAGTTTTTTCCCTTTTGACTCAGGTGGCCGGAAGGGCGGGGAGATCGAA
ACTCAAAGGGGAAGTTTTGATCGAAACGAACGCTCCCAATCACCCTGTGATTCAAATGGCGACAAGTCAAAACTATATTC
AATTTTATGAATCTGAAATTCCGGCAAGAAAAGAACTTTTTTATCCTCCGTTTTCCAGGCTTGTCCGTATCGTTTCCAGA
TCCAAAGACGAACGGATTTCTCTTGAAACGATCGAACTCGTATTTGGAGTTCTCAAAAAATTTTTTCCTTCCAAAGATAC
GGTTTTACTCGGGCCCGCGCCTTGTCCTTTTTATAGAATCGATTCCAATTTCAGGAATCATATCATTCTCAAAACCACTT
CTCCGGGCGATTGGAGGGAAATTTTTAAAAAAGAAGTTCGTCCTCTGAAACTTTCGAAAAAAGTGTATTTAGAAATTGAT
TTCGATCCTTTGGATCTGGTGTAA

Upstream 100 bases:

>100_bases
ATTCTTTTGAATTTGAGACGATCAAAAAAGAGGAGATCTCGGTTAAGGAAAGTATCCTTTCTCAACTCTCTCTTTTCCAA
AACAAACTCAAGGATATACT

Downstream 100 bases:

>100_bases
CATGAAAATCGGATACTTTGGAACTCCAGAACATTCCGCAAAACTTTTAAAAGCTCTGATCGATTCTACACTTGCTGAAG
TTTTGTTTGTCGTCACCAAT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MIYYAEVAFDLPVEEDTFTYEVPQNTPIGVRVLAKLRNREEEGIIVSIHQNEPNYKVLPVVKIIDKAPIVLGEQIDLAYW
MKDQYIASLGECIYKMIPAGRRQVKLEVFPSDSEGKPVTLNEEQEVATQNILSTFGQTAVHLLFGITGSGKTEVYIHLIQ
KALETPNRSVILLVPEISLTFHIIRKLELIFPGQLAVLHSALKISEKFKAYNELLSGKKRVAVGTRSAVFAPVSNLGLVI
IDEDHDSSFKEHSSPRYHARQVAMQRCKTNNAVLVMGTATPSLEIYHLAKEKRIYLHTLTKRPEGVLPPTVRIVENKKES
NVISSELSFEIKRRLDKKEQVILLLNRRGYSPLIHSSSTSSYIPCPNCTTNLCYHKKGTAVCHLCGHTENLDSLEKRMGE
RFTLKGTGTQKLEENLLEAFPQARVERLDQDSIQDRSLLNEVIARLLGGEIDILTGTQMIAKGLDASQVTLVGVLNAGIG
LGLPDFRANERVFSLLTQVAGRAGRSKLKGEVLIETNAPNHPVIQMATSQNYIQFYESEIPARKELFYPPFSRLVRIVSR
SKDERISLETIELVFGVLKKFFPSKDTVLLGPAPCPFYRIDSNFRNHIILKTTSPGDWREIFKKEVRPLKLSKKVYLEID
FDPLDLV

Sequences:

>Translated_647_residues
MIYYAEVAFDLPVEEDTFTYEVPQNTPIGVRVLAKLRNREEEGIIVSIHQNEPNYKVLPVVKIIDKAPIVLGEQIDLAYW
MKDQYIASLGECIYKMIPAGRRQVKLEVFPSDSEGKPVTLNEEQEVATQNILSTFGQTAVHLLFGITGSGKTEVYIHLIQ
KALETPNRSVILLVPEISLTFHIIRKLELIFPGQLAVLHSALKISEKFKAYNELLSGKKRVAVGTRSAVFAPVSNLGLVI
IDEDHDSSFKEHSSPRYHARQVAMQRCKTNNAVLVMGTATPSLEIYHLAKEKRIYLHTLTKRPEGVLPPTVRIVENKKES
NVISSELSFEIKRRLDKKEQVILLLNRRGYSPLIHSSSTSSYIPCPNCTTNLCYHKKGTAVCHLCGHTENLDSLEKRMGE
RFTLKGTGTQKLEENLLEAFPQARVERLDQDSIQDRSLLNEVIARLLGGEIDILTGTQMIAKGLDASQVTLVGVLNAGIG
LGLPDFRANERVFSLLTQVAGRAGRSKLKGEVLIETNAPNHPVIQMATSQNYIQFYESEIPARKELFYPPFSRLVRIVSR
SKDERISLETIELVFGVLKKFFPSKDTVLLGPAPCPFYRIDSNFRNHIILKTTSPGDWREIFKKEVRPLKLSKKVYLEID
FDPLDLV
>Mature_647_residues
MIYYAEVAFDLPVEEDTFTYEVPQNTPIGVRVLAKLRNREEEGIIVSIHQNEPNYKVLPVVKIIDKAPIVLGEQIDLAYW
MKDQYIASLGECIYKMIPAGRRQVKLEVFPSDSEGKPVTLNEEQEVATQNILSTFGQTAVHLLFGITGSGKTEVYIHLIQ
KALETPNRSVILLVPEISLTFHIIRKLELIFPGQLAVLHSALKISEKFKAYNELLSGKKRVAVGTRSAVFAPVSNLGLVI
IDEDHDSSFKEHSSPRYHARQVAMQRCKTNNAVLVMGTATPSLEIYHLAKEKRIYLHTLTKRPEGVLPPTVRIVENKKES
NVISSELSFEIKRRLDKKEQVILLLNRRGYSPLIHSSSTSSYIPCPNCTTNLCYHKKGTAVCHLCGHTENLDSLEKRMGE
RFTLKGTGTQKLEENLLEAFPQARVERLDQDSIQDRSLLNEVIARLLGGEIDILTGTQMIAKGLDASQVTLVGVLNAGIG
LGLPDFRANERVFSLLTQVAGRAGRSKLKGEVLIETNAPNHPVIQMATSQNYIQFYESEIPARKELFYPPFSRLVRIVSR
SKDERISLETIELVFGVLKKFFPSKDTVLLGPAPCPFYRIDSNFRNHIILKTTSPGDWREIFKKEVRPLKLSKKVYLEID
FDPLDLV

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=541, Percent_Identity=32.1626617375231, Blast_Score=281, Evalue=9e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 72896; Mature: 72896

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYYAEVAFDLPVEEDTFTYEVPQNTPIGVRVLAKLRNREEEGIIVSIHQNEPNYKVLPV
CEEEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEHH
VKIIDKAPIVLGEQIDLAYWMKDQYIASLGECIYKMIPAGRRQVKLEVFPSDSEGKPVTL
HHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEE
NEEQEVATQNILSTFGQTAVHLLFGITGSGKTEVYIHLIQKALETPNRSVILLVPEISLT
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHH
FHIIRKLELIFPGQLAVLHSALKISEKFKAYNELLSGKKRVAVGTRSAVFAPVSNLGLVI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEE
IDEDHDSSFKEHSSPRYHARQVAMQRCKTNNAVLVMGTATPSLEIYHLAKEKRIYLHTLT
EECCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEEEEEEEC
KRPEGVLPPTVRIVENKKESNVISSELSFEIKRRLDKKEQVILLLNRRGYSPLIHSSSTS
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCC
SYIPCPNCTTNLCYHKKGTAVCHLCGHTENLDSLEKRMGERFTLKGTGTQKLEENLLEAF
CCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
PQARVERLDQDSIQDRSLLNEVIARLLGGEIDILTGTQMIAKGLDASQVTLVGVLNAGIG
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCEEEEEEECCCCC
LGLPDFRANERVFSLLTQVAGRAGRSKLKGEVLIETNAPNHPVIQMATSQNYIQFYESEI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHCC
PARKELFYPPFSRLVRIVSRSKDERISLETIELVFGVLKKFFPSKDTVLLGPAPCPFYRI
CCCHHCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEE
DSNFRNHIILKTTSPGDWREIFKKEVRPLKLSKKVYLEIDFDPLDLV
CCCCCCEEEEEECCCCHHHHHHHHCCCCEEECCEEEEEECCCHHCCC
>Mature Secondary Structure
MIYYAEVAFDLPVEEDTFTYEVPQNTPIGVRVLAKLRNREEEGIIVSIHQNEPNYKVLPV
CEEEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEHH
VKIIDKAPIVLGEQIDLAYWMKDQYIASLGECIYKMIPAGRRQVKLEVFPSDSEGKPVTL
HHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEE
NEEQEVATQNILSTFGQTAVHLLFGITGSGKTEVYIHLIQKALETPNRSVILLVPEISLT
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHH
FHIIRKLELIFPGQLAVLHSALKISEKFKAYNELLSGKKRVAVGTRSAVFAPVSNLGLVI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEE
IDEDHDSSFKEHSSPRYHARQVAMQRCKTNNAVLVMGTATPSLEIYHLAKEKRIYLHTLT
EECCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEEEEEEEC
KRPEGVLPPTVRIVENKKESNVISSELSFEIKRRLDKKEQVILLLNRRGYSPLIHSSSTS
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCC
SYIPCPNCTTNLCYHKKGTAVCHLCGHTENLDSLEKRMGERFTLKGTGTQKLEENLLEAF
CCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
PQARVERLDQDSIQDRSLLNEVIARLLGGEIDILTGTQMIAKGLDASQVTLVGVLNAGIG
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCEEEEEEECCCCC
LGLPDFRANERVFSLLTQVAGRAGRSKLKGEVLIETNAPNHPVIQMATSQNYIQFYESEI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHCC
PARKELFYPPFSRLVRIVSRSKDERISLETIELVFGVLKKFFPSKDTVLLGPAPCPFYRI
CCCHHCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEE
DSNFRNHIILKTTSPGDWREIFKKEVRPLKLSKKVYLEIDFDPLDLV
CCCCCCEEEEEECCCCHHHHHHHHCCCCEEECCEEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]