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The map label for this gene is gspF [H]

Identifier: 116328307

GI number: 116328307

Start: 1924809

End: 1926035

Strand: Reverse

Name: gspF [H]

Synonym: LBL_1643

Alternate gene names: 116328307

Gene position: 1926035-1924809 (Counterclockwise)

Preceding gene: 116328308

Following gene: 116328306

Centisome position: 53.29

GC content: 42.22

Gene sequence:

>1227_bases
ATGGCCATTTATTCTTACGTTGCATTCAACAAAAAGGGAAAGGAAGAAAAGGGAATCATAGACGCGGCTTCTCTTCAGGC
TGCGAGATCCAAGTTAAAGAACAAGGGGTTGTATGTTCGCAATATCTCTGAAGATTCCGAACGAAAAGATAGGGAACTTT
TTCCATTTCTAGCAAAATATTTTTATAGAATTCCTCGTAAAGAAGTCGGGCTTTTTTCCAGACAACTCGCCACTTTACTC
GGTGCCGGAATTCCTCTGGATAAATCCCTTTCCAGCATCGTAGAACAAACGGAAAACCAAAATTTCAGAAAAGTTCTTAC
CGGAATGCAGGCAAATATCACGGAGGGTTCCTCCCTATCGGAAGCGATGAAAAAACATCCCGACGTGTTTCCCAGCCAGT
TTCCGTCTCTTGTCGCAGTCGGTGAAAAAACAGGGGATTACGAAGCCACTTTGACTCGTCTTGCTGAACTCGAAGAAAAA
TCGAGCGAACTCAAAGCAAAGGTTCAAGTTGCGATGGTATACCCTTTCATCATGGGCTCTTTATCCATCTTCGTTACGAT
CTTTTTATTAACGGTAGTTATTCCGCAGATTCAAGAATTGTTTTTACAGTTCGACGCGAAACTTCCTTTGATCACTCGAA
TCGTAATCGGAGTTTCGGACATTCTGATCGGATTTTGGTGGCTTCTTCTTGCCTTGGGTTTCGGCACCGTTATGAGTTTT
ATCTATTATAAAAACACTCCTAAAGGAAAACGGAATTGGGACGAGTTCGTATTGAAGATTCCGATTTTGGGTTCTCTTGC
ACGCAAGGTTCTCGTGAGCAGTTTTGCAAGAAACATCGGAATTCTTTTGAGTAACCGCGTTCCCTTGATTACTACGTTAA
TTATCGTGGAAAAAATCGTGAATCATTCCATCTTCGGAGAAGAAATTAAAAACGCGGTAGAAAGAATCAAGGAAGGAGAA
AAACTTTCCGCATCTTTCAGCGGGTCCGTAATTCTGCCTCAGATGGTGGTGGGTATGATTGCCGCAGGAGAGGTTTCGGA
CCGGGTTCCGGAAATGATGAATAAACTTGCGGATATCTACGATACCGAAGTAGATACCGCGATTAAAACGATGACACAAT
CTATGGAACCTTTGATGATCGTAGTAATGGGACTTCTCATTGGAACGATCATGGCATCCATTATGGTCCCAATGTACAAC
TTGACACAACAACTTCAAAACATATAA

Upstream 100 bases:

>100_bases
GCGCTCGAACATAATTTCCAAACTCTAAAAGATTACGGAGTCAAAAAGGTAATCGACGGAGTAACTACGATCGACGAAGT
CCTCAGAGTAACCTAATTTC

Downstream 100 bases:

>100_bases
CTTTAGATTAAGGAGAATTGAATTGAACCGATCCAAATTAAAAAGAAAATACAGAAAAGGTCTGACACTGATCGAACTCG
CTGTTGTTGTGATCATCCTA

Product: Type II secretory pathway component, protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MAIYSYVAFNKKGKEEKGIIDAASLQAARSKLKNKGLYVRNISEDSERKDRELFPFLAKYFYRIPRKEVGLFSRQLATLL
GAGIPLDKSLSSIVEQTENQNFRKVLTGMQANITEGSSLSEAMKKHPDVFPSQFPSLVAVGEKTGDYEATLTRLAELEEK
SSELKAKVQVAMVYPFIMGSLSIFVTIFLLTVVIPQIQELFLQFDAKLPLITRIVIGVSDILIGFWWLLLALGFGTVMSF
IYYKNTPKGKRNWDEFVLKIPILGSLARKVLVSSFARNIGILLSNRVPLITTLIIVEKIVNHSIFGEEIKNAVERIKEGE
KLSASFSGSVILPQMVVGMIAAGEVSDRVPEMMNKLADIYDTEVDTAIKTMTQSMEPLMIVVMGLLIGTIMASIMVPMYN
LTQQLQNI

Sequences:

>Translated_408_residues
MAIYSYVAFNKKGKEEKGIIDAASLQAARSKLKNKGLYVRNISEDSERKDRELFPFLAKYFYRIPRKEVGLFSRQLATLL
GAGIPLDKSLSSIVEQTENQNFRKVLTGMQANITEGSSLSEAMKKHPDVFPSQFPSLVAVGEKTGDYEATLTRLAELEEK
SSELKAKVQVAMVYPFIMGSLSIFVTIFLLTVVIPQIQELFLQFDAKLPLITRIVIGVSDILIGFWWLLLALGFGTVMSF
IYYKNTPKGKRNWDEFVLKIPILGSLARKVLVSSFARNIGILLSNRVPLITTLIIVEKIVNHSIFGEEIKNAVERIKEGE
KLSASFSGSVILPQMVVGMIAAGEVSDRVPEMMNKLADIYDTEVDTAIKTMTQSMEPLMIVVMGLLIGTIMASIMVPMYN
LTQQLQNI
>Mature_407_residues
AIYSYVAFNKKGKEEKGIIDAASLQAARSKLKNKGLYVRNISEDSERKDRELFPFLAKYFYRIPRKEVGLFSRQLATLLG
AGIPLDKSLSSIVEQTENQNFRKVLTGMQANITEGSSLSEAMKKHPDVFPSQFPSLVAVGEKTGDYEATLTRLAELEEKS
SELKAKVQVAMVYPFIMGSLSIFVTIFLLTVVIPQIQELFLQFDAKLPLITRIVIGVSDILIGFWWLLLALGFGTVMSFI
YYKNTPKGKRNWDEFVLKIPILGSLARKVLVSSFARNIGILLSNRVPLITTLIIVEKIVNHSIFGEEIKNAVERIKEGEK
LSASFSGSVILPQMVVGMIAAGEVSDRVPEMMNKLADIYDTEVDTAIKTMTQSMEPLMIVVMGLLIGTIMASIMVPMYNL
TQQLQNI

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=399, Percent_Identity=30.8270676691729, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786295, Length=329, Percent_Identity=23.1003039513678, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 45507; Mature: 45376

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIYSYVAFNKKGKEEKGIIDAASLQAARSKLKNKGLYVRNISEDSERKDRELFPFLAKY
CCEEHEEHCCCCCCHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHH
FYRIPRKEVGLFSRQLATLLGAGIPLDKSLSSIVEQTENQNFRKVLTGMQANITEGSSLS
HHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHH
EAMKKHPDVFPSQFPSLVAVGEKTGDYEATLTRLAELEEKSSELKAKVQVAMVYPFIMGS
HHHHHCCCCCHHHCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSIFVTIFLLTVVIPQIQELFLQFDAKLPLITRIVIGVSDILIGFWWLLLALGFGTVMSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYYKNTPKGKRNWDEFVLKIPILGSLARKVLVSSFARNIGILLSNRVPLITTLIIVEKIV
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
NHSIFGEEIKNAVERIKEGEKLSASFSGSVILPQMVVGMIAAGEVSDRVPEMMNKLADIY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DTEVDTAIKTMTQSMEPLMIVVMGLLIGTIMASIMVPMYNLTQQLQNI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIYSYVAFNKKGKEEKGIIDAASLQAARSKLKNKGLYVRNISEDSERKDRELFPFLAKY
CEEHEEHCCCCCCHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHH
FYRIPRKEVGLFSRQLATLLGAGIPLDKSLSSIVEQTENQNFRKVLTGMQANITEGSSLS
HHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHH
EAMKKHPDVFPSQFPSLVAVGEKTGDYEATLTRLAELEEKSSELKAKVQVAMVYPFIMGS
HHHHHCCCCCHHHCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSIFVTIFLLTVVIPQIQELFLQFDAKLPLITRIVIGVSDILIGFWWLLLALGFGTVMSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYYKNTPKGKRNWDEFVLKIPILGSLARKVLVSSFARNIGILLSNRVPLITTLIIVEKIV
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
NHSIFGEEIKNAVERIKEGEKLSASFSGSVILPQMVVGMIAAGEVSDRVPEMMNKLADIY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DTEVDTAIKTMTQSMEPLMIVVMGLLIGTIMASIMVPMYNLTQQLQNI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]