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The map label for this gene is purH

Identifier: 116328230

GI number: 116328230

Start: 1817134

End: 1818669

Strand: Reverse

Name: purH

Synonym: LBL_1554

Alternate gene names: 116328230

Gene position: 1818669-1817134 (Counterclockwise)

Preceding gene: 116328231

Following gene: 116328229

Centisome position: 50.32

GC content: 44.01

Gene sequence:

>1536_bases
ATGATACAAATTAAGAGAGCTCTTATTTCCGTTAGCGACAAATCCGATATAGTAGAATTTGCACAATTTCTAAATCAAAA
CGGCGTTGAAATCATCTCCACCGGCGGAACGTTAAAACTTTTAAAAGACAACGGGATCCAAGCAATCGCGATCGACGATT
ACACCGGTTTTCCGGAAATTTTAGAGGGAAGAGTCAAAACCCTCCACCCGAAAGTGCACGGAGGTTTACTCGGAGTCGTT
TCCAATCCAGCTCACAAACAGAAGATGGAAGAATTAAAAATTCCTAAAATAGATTTGGTAGTCGTAAACTTGTATCCGTT
TTTAAAGACGGTTTCCAAACCGGGAGTTCAATTGGAGGAAGCAATCGAGAATATCGATATCGGTGGGCCTTCCATGATTC
GAAGTGCGGCTAAGAATTATAAACACACTCTCGTTCTCACTGATCCGAGCGATTACGAGGAAGTACGAGTTTTGATTGCG
TCCGGAGGAATTTCGGAAGAAGTGGCCGCGGGTTATATGAGAAAAGCATTCTCTCATACTGCGATGTATGATACCGCAAT
TTCATCCTGGTTCCACAAACAAGCCGGAGACGTTTTCCCGGATGTTCTCAATCTTTCCTTCTTAAAAAAACAAAAACTCA
GATATGGGGAAAATCCCCATCAAGCCGCGTCTTTTTACGAACCCCTTTTTGTGAAGAGTGATTTTTCTCCTTTACAAGGA
AAGGAACTGTCGTTTAACAATATGCTGGATTTCGATGCGGCGTTTCACATCTCTAGCCTACTTCCGGAAAACACGGTTTG
TATCATCAAACATCTTAATCCTTGCGGGATTGCATATGCGGACGATCCTTTGGAAGCGTTTCAGCTTGCAAGAAGAACCG
ACCCGATTTCCGCATTCGGCGGAGTAATTGGAATCAAAGGAATCGTACATGGAGAGTTGGCTACGGCCATCACTGAAAAC
TTCGTAGAAGGTGTGATCGCTCAAAAGTTCACTCCGGAGGCTCTGGAGCTTTTTTCTAAAAAGCCGAATATCCGTCTGAT
TGAAATTGAAAACTTCAAAGAGGCGCTCGACGAATTGGATCTAAGACCCATCCATCACGGTTTGCTCATACAAGAGAGAG
ATTATACTACGATTACAGAAAAAGATCTAAAAGTAGTTACTAAAAAACAACCTACGTCCGATGATATTCGTGGTTTGATG
TTTGCTTGGTCTTGTGTTCGTTTTATCAAATCCAATGCGATCGTCTATACGGAAGAAAATGCAACTCTTGGAATTGGCGC
GGGGCAGATGTCTAGAGTTGACTCGGTGCAGTTAGGCGCTAACAAGGCGTTAAACGTCGGTTTATCCGTAGTTGGTTCCT
ATGTTGCAAGCGACGCATTCTTCCCTTTTAGAGACGGGATTGATGCTCTTGCAAAAGCCGGAGCGAAAGCGATCATCCAA
CCTGGCGGTTCGGTTCGAGATGCGGAAGTGATTCAAGCGGCGGACGAACACGGACTCATTATGGTCTTTACGGGGATGAG
GCATTTCAGACACTGA

Upstream 100 bases:

>100_bases
GATTCTCAAAGAGGAACATAAAATCCTACCACTCGCGGTTCAATACTTTTGCGAGGATAGGCTTAAGATCCATAATAGAA
AGGTAAGCATTGAGTAAGTA

Downstream 100 bases:

>100_bases
TATGGAATTTTTACTTTATTTAATCTTCTTCGGAATCGTTTCGGGAGTACTTGTTCTCGCGGATTATTATTTTAAACTTC
TGTTTCTTTCCGGAAGAGAA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MIQIKRALISVSDKSDIVEFAQFLNQNGVEIISTGGTLKLLKDNGIQAIAIDDYTGFPEILEGRVKTLHPKVHGGLLGVV
SNPAHKQKMEELKIPKIDLVVVNLYPFLKTVSKPGVQLEEAIENIDIGGPSMIRSAAKNYKHTLVLTDPSDYEEVRVLIA
SGGISEEVAAGYMRKAFSHTAMYDTAISSWFHKQAGDVFPDVLNLSFLKKQKLRYGENPHQAASFYEPLFVKSDFSPLQG
KELSFNNMLDFDAAFHISSLLPENTVCIIKHLNPCGIAYADDPLEAFQLARRTDPISAFGGVIGIKGIVHGELATAITEN
FVEGVIAQKFTPEALELFSKKPNIRLIEIENFKEALDELDLRPIHHGLLIQERDYTTITEKDLKVVTKKQPTSDDIRGLM
FAWSCVRFIKSNAIVYTEENATLGIGAGQMSRVDSVQLGANKALNVGLSVVGSYVASDAFFPFRDGIDALAKAGAKAIIQ
PGGSVRDAEVIQAADEHGLIMVFTGMRHFRH

Sequences:

>Translated_511_residues
MIQIKRALISVSDKSDIVEFAQFLNQNGVEIISTGGTLKLLKDNGIQAIAIDDYTGFPEILEGRVKTLHPKVHGGLLGVV
SNPAHKQKMEELKIPKIDLVVVNLYPFLKTVSKPGVQLEEAIENIDIGGPSMIRSAAKNYKHTLVLTDPSDYEEVRVLIA
SGGISEEVAAGYMRKAFSHTAMYDTAISSWFHKQAGDVFPDVLNLSFLKKQKLRYGENPHQAASFYEPLFVKSDFSPLQG
KELSFNNMLDFDAAFHISSLLPENTVCIIKHLNPCGIAYADDPLEAFQLARRTDPISAFGGVIGIKGIVHGELATAITEN
FVEGVIAQKFTPEALELFSKKPNIRLIEIENFKEALDELDLRPIHHGLLIQERDYTTITEKDLKVVTKKQPTSDDIRGLM
FAWSCVRFIKSNAIVYTEENATLGIGAGQMSRVDSVQLGANKALNVGLSVVGSYVASDAFFPFRDGIDALAKAGAKAIIQ
PGGSVRDAEVIQAADEHGLIMVFTGMRHFRH
>Mature_511_residues
MIQIKRALISVSDKSDIVEFAQFLNQNGVEIISTGGTLKLLKDNGIQAIAIDDYTGFPEILEGRVKTLHPKVHGGLLGVV
SNPAHKQKMEELKIPKIDLVVVNLYPFLKTVSKPGVQLEEAIENIDIGGPSMIRSAAKNYKHTLVLTDPSDYEEVRVLIA
SGGISEEVAAGYMRKAFSHTAMYDTAISSWFHKQAGDVFPDVLNLSFLKKQKLRYGENPHQAASFYEPLFVKSDFSPLQG
KELSFNNMLDFDAAFHISSLLPENTVCIIKHLNPCGIAYADDPLEAFQLARRTDPISAFGGVIGIKGIVHGELATAITEN
FVEGVIAQKFTPEALELFSKKPNIRLIEIENFKEALDELDLRPIHHGLLIQERDYTTITEKDLKVVTKKQPTSDDIRGLM
FAWSCVRFIKSNAIVYTEENATLGIGAGQMSRVDSVQLGANKALNVGLSVVGSYVASDAFFPFRDGIDALAKAGAKAIIQ
PGGSVRDAEVIQAADEHGLIMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=470, Percent_Identity=34.8936170212766, Blast_Score=238, Evalue=9e-63,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=48.952380952381, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71985564, Length=599, Percent_Identity=30.550918196995, Blast_Score=252, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI71985574, Length=340, Percent_Identity=24.4117647058824, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71985556, Length=61, Percent_Identity=45.9016393442623, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=472, Percent_Identity=34.9576271186441, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6323056, Length=475, Percent_Identity=34.1052631578947, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24649832, Length=481, Percent_Identity=33.2640332640333, Blast_Score=231, Evalue=1e-60,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_LEPBJ (Q04T56)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800672.1
- ProteinModelPortal:   Q04T56
- SMR:   Q04T56
- STRING:   Q04T56
- GeneID:   4410296
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1329
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- PhylomeDB:   Q04T56
- ProtClustDB:   PRK00881
- BioCyc:   LBOR355277:LBJ_1329-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 56137; Mature: 56137

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIKRALISVSDKSDIVEFAQFLNQNGVEIISTGGTLKLLKDNGIQAIAIDDYTGFPEI
CEEEEEHHHCCCCHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCEEEEEECCCCCCHHH
LEGRVKTLHPKVHGGLLGVVSNPAHKQKMEELKIPKIDLVVVNLYPFLKTVSKPGVQLEE
HHCHHHHCCCHHCCCEEEECCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCHHHH
AIENIDIGGPSMIRSAAKNYKHTLVLTDPSDYEEVRVLIASGGISEEVAAGYMRKAFSHT
HHHHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHH
AMYDTAISSWFHKQAGDVFPDVLNLSFLKKQKLRYGENPHQAASFYEPLFVKSDFSPLQG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCCCCCC
KELSFNNMLDFDAAFHISSLLPENTVCIIKHLNPCGIAYADDPLEAFQLARRTDPISAFG
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCHHHHHHHHHCCCCHHHHC
GVIGIKGIVHGELATAITENFVEGVIAQKFTPEALELFSKKPNIRLIEIENFKEALDELD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEECCHHHHHHHCC
LRPIHHGLLIQERDYTTITEKDLKVVTKKQPTSDDIRGLMFAWSCVRFIKSNAIVYTEEN
CCCCCCCEEEECCCCCEECCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECC
ATLGIGAGQMSRVDSVQLGANKALNVGLSVVGSYVASDAFFPFRDGIDALAKAGAKAIIQ
CEEEECCCCHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCCCEEEC
PGGSVRDAEVIQAADEHGLIMVFTGMRHFRH
CCCCCCHHHHHHHCCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MIQIKRALISVSDKSDIVEFAQFLNQNGVEIISTGGTLKLLKDNGIQAIAIDDYTGFPEI
CEEEEEHHHCCCCHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCEEEEEECCCCCCHHH
LEGRVKTLHPKVHGGLLGVVSNPAHKQKMEELKIPKIDLVVVNLYPFLKTVSKPGVQLEE
HHCHHHHCCCHHCCCEEEECCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCHHHH
AIENIDIGGPSMIRSAAKNYKHTLVLTDPSDYEEVRVLIASGGISEEVAAGYMRKAFSHT
HHHHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHH
AMYDTAISSWFHKQAGDVFPDVLNLSFLKKQKLRYGENPHQAASFYEPLFVKSDFSPLQG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCCCCCC
KELSFNNMLDFDAAFHISSLLPENTVCIIKHLNPCGIAYADDPLEAFQLARRTDPISAFG
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCHHHHHHHHHCCCCHHHHC
GVIGIKGIVHGELATAITENFVEGVIAQKFTPEALELFSKKPNIRLIEIENFKEALDELD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEECCHHHHHHHCC
LRPIHHGLLIQERDYTTITEKDLKVVTKKQPTSDDIRGLMFAWSCVRFIKSNAIVYTEEN
CCCCCCCEEEECCCCCEECCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECC
ATLGIGAGQMSRVDSVQLGANKALNVGLSVVGSYVASDAFFPFRDGIDALAKAGAKAIIQ
CEEEECCCCHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCCCEEEC
PGGSVRDAEVIQAADEHGLIMVFTGMRHFRH
CCCCCCHHHHHHHCCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA