Click here to switch to the map view.

The map label for this gene is lipA

Identifier: 116328223

GI number: 116328223

Start: 1810286

End: 1811194

Strand: Reverse

Name: lipA

Synonym: LBL_1547

Alternate gene names: 116328223

Gene position: 1811194-1810286 (Counterclockwise)

Preceding gene: 116328227

Following gene: 116328222

Centisome position: 50.11

GC content: 43.56

Gene sequence:

>909_bases
ATGATAAATCCGCTTAAAAAGAAACCTCGCACTCACTTTCTCCAAAAAGCTCCTGAAAAACCGGATTGGCTCAAAGTAAA
ACTTACTTTTCCCGACCCAAAAAACAATCCAGTTGCCATAGTTCGGAATTCTCTGGAGAAAAAAAAACTCAACACTGTCT
GCGAAAGTGCTTCTTGTCCGAATCTAAACCATTGCTGGTCCAGAAAGACGGCGACCTATATGCTCGGAGGAGATATTTGT
ACGAGACGTTGCTCTTATTGTGATGTTGCTTCCGGTAAGCCCTCCGCTTTGGATCGGGACGAACCCAAACGAGTAGCCGA
ATCGGCAATCGCTCTTGGCCTAAAACACGTGGTAATTACCGCCGTGAATCGAGACGATTTAGAAGATGGTGGTGCGGCTC
ACTTTGCTGAAACGGTGGAAGTAGTAAGAGAAGGACTTCCGGATTGTAAGATCGAGCTGTTAGTACCGGATTTTAAGGTT
CGACCAGAATCTTTGGAAATTATTTTTCAGTGTAAACCGGATATTTTCAATCACAACGTGGAAACCATAAAACGCTTATT
TCCCGAAGTTGCCCCTCAGAAAAAATACGAACGTTCTCTCGACGTTCTAAAAATCGCCTCCGAAAAAGGGTTTCTTACAA
AGAGTGGATTGATTTTAGGAATGGGTGAAACCGTGGAAGAAGTGAAGGAATGTATGAGAGATTTAATCGGGGTCGGAGTT
TCCTTGCTGACACTCGGACAATACCTTCAACCGACGCCGACTCATCTTCCCGTTAAAAGTTATGTTCTTCCAGAGGTGTT
TCAAGAATTGAGAATTTACGGTAAGTCTATCGGATTCAAAGGAGTTTTTTCGGGTCCTCTAGTCAGAAGTTCTTATCACG
CAGATGAGCAAGTCTCATGGAATCCCTAA

Upstream 100 bases:

>100_bases
TTCGAAAAGACTCAAAAAATCTTCTATCCAAAACTTTCATTGACCCGAACTCTCTCTCATACGAAAATAGAAGTAGAAAC
TCTGTGCTCATTCGAAACAT

Downstream 100 bases:

>100_bases
AAAACTTTGGTCCTTCTCCCGAAGAGATTAAGAAACTTATCTATCATTCTATGATTCAATTTCTTTCTAATCAAGAAGGT
CCTGTCAGTAGATTCGAAGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MINPLKKKPRTHFLQKAPEKPDWLKVKLTFPDPKNNPVAIVRNSLEKKKLNTVCESASCPNLNHCWSRKTATYMLGGDIC
TRRCSYCDVASGKPSALDRDEPKRVAESAIALGLKHVVITAVNRDDLEDGGAAHFAETVEVVREGLPDCKIELLVPDFKV
RPESLEIIFQCKPDIFNHNVETIKRLFPEVAPQKKYERSLDVLKIASEKGFLTKSGLILGMGETVEEVKECMRDLIGVGV
SLLTLGQYLQPTPTHLPVKSYVLPEVFQELRIYGKSIGFKGVFSGPLVRSSYHADEQVSWNP

Sequences:

>Translated_302_residues
MINPLKKKPRTHFLQKAPEKPDWLKVKLTFPDPKNNPVAIVRNSLEKKKLNTVCESASCPNLNHCWSRKTATYMLGGDIC
TRRCSYCDVASGKPSALDRDEPKRVAESAIALGLKHVVITAVNRDDLEDGGAAHFAETVEVVREGLPDCKIELLVPDFKV
RPESLEIIFQCKPDIFNHNVETIKRLFPEVAPQKKYERSLDVLKIASEKGFLTKSGLILGMGETVEEVKECMRDLIGVGV
SLLTLGQYLQPTPTHLPVKSYVLPEVFQELRIYGKSIGFKGVFSGPLVRSSYHADEQVSWNP
>Mature_302_residues
MINPLKKKPRTHFLQKAPEKPDWLKVKLTFPDPKNNPVAIVRNSLEKKKLNTVCESASCPNLNHCWSRKTATYMLGGDIC
TRRCSYCDVASGKPSALDRDEPKRVAESAIALGLKHVVITAVNRDDLEDGGAAHFAETVEVVREGLPDCKIELLVPDFKV
RPESLEIIFQCKPDIFNHNVETIKRLFPEVAPQKKYERSLDVLKIASEKGFLTKSGLILGMGETVEEVKECMRDLIGVGV
SLLTLGQYLQPTPTHLPVKSYVLPEVFQELRIYGKSIGFKGVFSGPLVRSSYHADEQVSWNP

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=41.0526315789474, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=40.080971659919, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI1786846, Length=282, Percent_Identity=47.8723404255319, Blast_Score=274, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI32564533, Length=285, Percent_Identity=43.5087719298246, Blast_Score=224, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=305, Percent_Identity=39.672131147541, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI221513272, Length=285, Percent_Identity=43.1578947368421, Blast_Score=221, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_LEPBJ (Q04T63)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800665.1
- ProteinModelPortal:   Q04T63
- STRING:   Q04T63
- GeneID:   4410289
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1322
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   DPHEPQR
- PhylomeDB:   Q04T63
- ProtClustDB:   PRK05481
- BioCyc:   LBOR355277:LBJ_1322-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33784; Mature: 33784

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPLKKKPRTHFLQKAPEKPDWLKVKLTFPDPKNNPVAIVRNSLEKKKLNTVCESASCP
CCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCC
NLNHCWSRKTATYMLGGDICTRRCSYCDVASGKPSALDRDEPKRVAESAIALGLKHVVIT
CCHHHHCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEE
AVNRDDLEDGGAAHFAETVEVVREGLPDCKIELLVPDFKVRPESLEIIFQCKPDIFNHNV
ECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCEEEEEEECCCHHCCCH
ETIKRLFPEVAPQKKYERSLDVLKIASEKGFLTKSGLILGMGETVEEVKECMRDLIGVGV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCEEEECCHHHHHHHHHHHHHHHHHH
SLLTLGQYLQPTPTHLPVKSYVLPEVFQELRIYGKSIGFKGVFSGPLVRSSYHADEQVSW
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
NP
CC
>Mature Secondary Structure
MINPLKKKPRTHFLQKAPEKPDWLKVKLTFPDPKNNPVAIVRNSLEKKKLNTVCESASCP
CCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCC
NLNHCWSRKTATYMLGGDICTRRCSYCDVASGKPSALDRDEPKRVAESAIALGLKHVVIT
CCHHHHCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEE
AVNRDDLEDGGAAHFAETVEVVREGLPDCKIELLVPDFKVRPESLEIIFQCKPDIFNHNV
ECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCEEEEEEECCCHHCCCH
ETIKRLFPEVAPQKKYERSLDVLKIASEKGFLTKSGLILGMGETVEEVKECMRDLIGVGV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCEEEECCHHHHHHHHHHHHHHHHHH
SLLTLGQYLQPTPTHLPVKSYVLPEVFQELRIYGKSIGFKGVFSGPLVRSSYHADEQVSW
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
NP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA