Click here to switch to the map view.

The map label for this gene is ptsA [H]

Identifier: 116328215

GI number: 116328215

Start: 1799903

End: 1801645

Strand: Reverse

Name: ptsA [H]

Synonym: LBL_1539

Alternate gene names: 116328215

Gene position: 1801645-1799903 (Counterclockwise)

Preceding gene: 116328216

Following gene: 116328211

Centisome position: 49.85

GC content: 40.28

Gene sequence:

>1743_bases
ATGATCTCCTCCAAACGTATAGTTTTTCCAGGAATTACAGCTTTTCCGGGGAAACTATATGGTAAAGTCTTAAAAACCGG
AAAAAAACGAAATACGATTCTCACCGGAACTTACATTCACGAATCCGAAAAAGAAGAAGAAATACAAAAATTCTCTATCG
CGTTGGAAGAAAGTCTTCAAAGTTTGCGGATTTTGATTGCCTCTCTTGAAACTTCCGGGCCGGATCAGAAAGAAATCCGG
GAGATTTTAGAAACACAGGCTATGATTTGCTCCGATCCGAGTCTTGCCACCTCGGTTCGAAAAAGAATTTTAGAATTCGG
AGAGAACGCGATTCTTGCCGTACAAAACGTAACTCACGAAATTTCCGAAAAGTTTAAAGCTATGGAGAATGAATTTTTCC
GGGAAAGAGTGGATCATTTTCAAGACGTCTCAAATCGATTGATCGAGTTCCTTGCGGGTAAAAAGGAAGAGGATTCTTTT
CTTTCCGGTTTAAAAGAGGATTTGATCTTGGTCGCAAGAGAACTTACTCCTTCTCAAATGATTCTTATGGATAAGACTCG
TATCCGCGGGATTGCGACCGACTTAGGTGGGAAAACGGGACATATGGCAATTCTAGCGAGAAATTACGGGATTCCTACTA
TCGTAGGGTTAAAGGATTTTTCCACACATGTCAAAGATAACGAGTTTATCTTTTTAGATGCGGAGTTAGGAAACGTAGTT
CGTTTTCCGACTTTGGAAGAAGTTAAGTATTACGGATTTTCCTCTTCTTATCCCACGGAAGAAAGTGAAACTAAAAAAAT
TAGAGCCATAAGTAAAGATGGGATTCGTGTTCGAATCAAGTGTAATCTTGAAACCGAACTCGATTGCGAACAGGCAATCA
AATTCGGAGCCGAAGGTGTGGGACTTTTTAGAAGTGAATCTTTATTTTTAAAATACCAAGACAGAAACGTTTCTGGCGAA
GAACAGTTTCTCGCTTATAAAGCAATCGCAGAAGGGATGCAAGATAAACCGGTTATCATCCGCACCTTCGACATTGGGGC
CGATAAGTTTTCAACGGGAGAACTCGAAGAAAATCCATTTTTAGGAAACCGAGGTATACGGTATTCACTTTCTCATTCGG
AATGGTTCGCCGAACAACTAACGGCGATTTTACGAGCCTCCGCTTTCGGGAATGTTAGTATTTTATTGCCGATGATTACC
GGTCCCGGGGAAATCGTTCGCACTCGCGAACTTTTGGAAGAATGCAAACGCAAATTAAGCGTTAAAAAGGAAAAATACAA
TAAAAAAATCAAAATCGGTGCGATGATTGAAACTCCCGCTGCGGTTGCCGCTTTAGATCTGATCGCGCGAGAAGCGGATT
TTTTTTCAGTGGGAACAAATGACCTACTTCAATACATAATGGCGGTGGATAGAAACAACATCCACGTTTCTTCTTTATAC
AATCCATATCATATCGCTTTTTTGAGAGCTTTGATCAAAATCGTGGAAGTTTCAAGAGATTACGACAAACCGCTCAGTCT
CTGCGGAGAACTTGCATCAGATACAGACTTTACGATCTTTTTAATCGGAATTGGAATTAGAGAGCTATCCGTTTCGATCC
CATTTTTAAACCCGATTCGAAAAATCGTCCGATCGATTTCTTTACACCAAGCAGGAATTTTAGTAAGAAAGATCTTAGAA
CTTTCCGAAGAAGAAAACTATGAAAGTATCGAAGCCTTCCTTTTCAGTAAGCACCTAAGTTAG

Upstream 100 bases:

>100_bases
TAAAAACTGAAACTTTATCTGTTGTAATTCTTACCTAGAAAAGAATTGTCAGATTCTTCTTTTGGGGAATTCTGTAGTCG
AGTTGAAACTCGTTTTTAGA

Downstream 100 bases:

>100_bases
CGTGAGTTCGACGTAGGATGGAAAAACGAGAAAGATCTTGTACTGAAAGTGCCATATCACAATATTAAACCGATCTTTCA
GCAAAAAGGGGCTAAATATG

Product: phosphoenolpyruvate--protein phosphatase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MISSKRIVFPGITAFPGKLYGKVLKTGKKRNTILTGTYIHESEKEEEIQKFSIALEESLQSLRILIASLETSGPDQKEIR
EILETQAMICSDPSLATSVRKRILEFGENAILAVQNVTHEISEKFKAMENEFFRERVDHFQDVSNRLIEFLAGKKEEDSF
LSGLKEDLILVARELTPSQMILMDKTRIRGIATDLGGKTGHMAILARNYGIPTIVGLKDFSTHVKDNEFIFLDAELGNVV
RFPTLEEVKYYGFSSSYPTEESETKKIRAISKDGIRVRIKCNLETELDCEQAIKFGAEGVGLFRSESLFLKYQDRNVSGE
EQFLAYKAIAEGMQDKPVIIRTFDIGADKFSTGELEENPFLGNRGIRYSLSHSEWFAEQLTAILRASAFGNVSILLPMIT
GPGEIVRTRELLEECKRKLSVKKEKYNKKIKIGAMIETPAAVAALDLIAREADFFSVGTNDLLQYIMAVDRNNIHVSSLY
NPYHIAFLRALIKIVEVSRDYDKPLSLCGELASDTDFTIFLIGIGIRELSVSIPFLNPIRKIVRSISLHQAGILVRKILE
LSEEENYESIEAFLFSKHLS

Sequences:

>Translated_580_residues
MISSKRIVFPGITAFPGKLYGKVLKTGKKRNTILTGTYIHESEKEEEIQKFSIALEESLQSLRILIASLETSGPDQKEIR
EILETQAMICSDPSLATSVRKRILEFGENAILAVQNVTHEISEKFKAMENEFFRERVDHFQDVSNRLIEFLAGKKEEDSF
LSGLKEDLILVARELTPSQMILMDKTRIRGIATDLGGKTGHMAILARNYGIPTIVGLKDFSTHVKDNEFIFLDAELGNVV
RFPTLEEVKYYGFSSSYPTEESETKKIRAISKDGIRVRIKCNLETELDCEQAIKFGAEGVGLFRSESLFLKYQDRNVSGE
EQFLAYKAIAEGMQDKPVIIRTFDIGADKFSTGELEENPFLGNRGIRYSLSHSEWFAEQLTAILRASAFGNVSILLPMIT
GPGEIVRTRELLEECKRKLSVKKEKYNKKIKIGAMIETPAAVAALDLIAREADFFSVGTNDLLQYIMAVDRNNIHVSSLY
NPYHIAFLRALIKIVEVSRDYDKPLSLCGELASDTDFTIFLIGIGIRELSVSIPFLNPIRKIVRSISLHQAGILVRKILE
LSEEENYESIEAFLFSKHLS
>Mature_580_residues
MISSKRIVFPGITAFPGKLYGKVLKTGKKRNTILTGTYIHESEKEEEIQKFSIALEESLQSLRILIASLETSGPDQKEIR
EILETQAMICSDPSLATSVRKRILEFGENAILAVQNVTHEISEKFKAMENEFFRERVDHFQDVSNRLIEFLAGKKEEDSF
LSGLKEDLILVARELTPSQMILMDKTRIRGIATDLGGKTGHMAILARNYGIPTIVGLKDFSTHVKDNEFIFLDAELGNVV
RFPTLEEVKYYGFSSSYPTEESETKKIRAISKDGIRVRIKCNLETELDCEQAIKFGAEGVGLFRSESLFLKYQDRNVSGE
EQFLAYKAIAEGMQDKPVIIRTFDIGADKFSTGELEENPFLGNRGIRYSLSHSEWFAEQLTAILRASAFGNVSILLPMIT
GPGEIVRTRELLEECKRKLSVKKEKYNKKIKIGAMIETPAAVAALDLIAREADFFSVGTNDLLQYIMAVDRNNIHVSSLY
NPYHIAFLRALIKIVEVSRDYDKPLSLCGELASDTDFTIFLIGIGIRELSVSIPFLNPIRKIVRSISLHQAGILVRKILE
LSEEENYESIEAFLFSKHLS

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=562, Percent_Identity=35.9430604982206, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1788726, Length=566, Percent_Identity=30.9187279151943, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI48994992, Length=464, Percent_Identity=34.9137931034483, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1789193, Length=534, Percent_Identity=33.3333333333333, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI1787994, Length=427, Percent_Identity=26.2295081967213, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI226510935, Length=214, Percent_Identity=26.1682242990654, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 65409; Mature: 65409

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSKRIVFPGITAFPGKLYGKVLKTGKKRNTILTGTYIHESEKEEEIQKFSIALEESLQ
CCCCCEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHH
SLRILIASLETSGPDQKEIREILETQAMICSDPSLATSVRKRILEFGENAILAVQNVTHE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHH
ISEKFKAMENEFFRERVDHFQDVSNRLIEFLAGKKEEDSFLSGLKEDLILVARELTPSQM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
ILMDKTRIRGIATDLGGKTGHMAILARNYGIPTIVGLKDFSTHVKDNEFIFLDAELGNVV
EEEEHHHHHEHHHHCCCCCCCEEEEEECCCCCEEEECHHHHHCCCCCCEEEEECCCCCEE
RFPTLEEVKYYGFSSSYPTEESETKKIRAISKDGIRVRIKCNLETELDCEQAIKFGAEGV
ECCCHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCCC
GLFRSESLFLKYQDRNVSGEEQFLAYKAIAEGMQDKPVIIRTFDIGADKFSTGELEENPF
CEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCC
LGNRGIRYSLSHSEWFAEQLTAILRASAFGNVSILLPMITGPGEIVRTRELLEECKRKLS
CCCCCCEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHC
VKKEKYNKKIKIGAMIETPAAVAALDLIAREADFFSVGTNDLLQYIMAVDRNNIHVSSLY
CHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHCCCCEEEEECC
NPYHIAFLRALIKIVEVSRDYDKPLSLCGELASDTDFTIFLIGIGIRELSVSIPFLNPIR
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEECHHHEEEECCCHHHHH
KIVRSISLHQAGILVRKILELSEEENYESIEAFLFSKHLS
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MISSKRIVFPGITAFPGKLYGKVLKTGKKRNTILTGTYIHESEKEEEIQKFSIALEESLQ
CCCCCEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHH
SLRILIASLETSGPDQKEIREILETQAMICSDPSLATSVRKRILEFGENAILAVQNVTHE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHH
ISEKFKAMENEFFRERVDHFQDVSNRLIEFLAGKKEEDSFLSGLKEDLILVARELTPSQM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
ILMDKTRIRGIATDLGGKTGHMAILARNYGIPTIVGLKDFSTHVKDNEFIFLDAELGNVV
EEEEHHHHHEHHHHCCCCCCCEEEEEECCCCCEEEECHHHHHCCCCCCEEEEECCCCCEE
RFPTLEEVKYYGFSSSYPTEESETKKIRAISKDGIRVRIKCNLETELDCEQAIKFGAEGV
ECCCHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCCC
GLFRSESLFLKYQDRNVSGEEQFLAYKAIAEGMQDKPVIIRTFDIGADKFSTGELEENPF
CEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCC
LGNRGIRYSLSHSEWFAEQLTAILRASAFGNVSILLPMITGPGEIVRTRELLEECKRKLS
CCCCCCEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHC
VKKEKYNKKIKIGAMIETPAAVAALDLIAREADFFSVGTNDLLQYIMAVDRNNIHVSSLY
CHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHCCCCEEEEECC
NPYHIAFLRALIKIVEVSRDYDKPLSLCGELASDTDFTIFLIGIGIRELSVSIPFLNPIR
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEECHHHEEEECCCHHHHH
KIVRSISLHQAGILVRKILELSEEENYESIEAFLFSKHLS
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]