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The map label for this gene is uvrD-1 [H]

Identifier: 116328207

GI number: 116328207

Start: 1790534

End: 1792726

Strand: Direct

Name: uvrD-1 [H]

Synonym: LBL_1531

Alternate gene names: 116328207

Gene position: 1790534-1792726 (Clockwise)

Preceding gene: 116328206

Following gene: 116328208

Centisome position: 49.54

GC content: 40.67

Gene sequence:

>2193_bases
TTGACTTCTTCTTTCCTTATAGGTCTGAACGAAGAACAAAAAAAAGCCGTTTTACAGATAACCGGCCCAGTACTCATCTT
AGCGGGCGCAGGCTCGGGCAAAACCAGAGTCATCACACATAGAATCGCAAACCTTCTAGTCAATCACGGAATTGATCGAA
TCTGCGCGTTAACCTTCACTAATAAGGCCGCCGCTGAGATGGTCGAACGAGTAAAAAACTTAGTCCCTTTCCTTCCGACA
AATCTTCAAATTAAAACATTCCATTCTCTTTGTCTTTATATCTTAAGGAGAGAAGCGTTTTTTTTTGGATTTGACAGCGC
GTTTACCGTTTACGATACGACCCTCCAAGAGTCCCTTCTCAAACAGGTCGTAAAAGACCTTTCCTTGGATCCTAAATTCT
ACAAACCGTCTATGCTCGGAAATTATATCAGCGGTTTGAAAGATAAGATGTTATCTCCCGAAACTTATTTGGAAAAGGAA
GGACGCACCGATTTTTCCAAAACCGTATCTGCAATTTATAAAGAATACGAAAAAAGAAAAGATGCGAACCGTGCTTTCGA
TTTCGGAGATCTCATTTGGAAAACGGTGCAGCTCTTTCAAAAATCCCCGGATGCAATCGCCAAATATCGTCATAAATGGG
AATACGTAATGGTAGACGAATATCAGGATACAAACAAAGTACAATACCAGTTAGTACTTCTTCTTGCAGGTGAAAAAAGA
AACCTTTGTGTTGTTGGCGACGACGACCAATCCATTTATTCCTGGAGAGGAGCCGACATCGGAAACATTCTGAATTTCGA
AAAGGACTTCCCCGAATCCACGGTCATAAAATTAGAAGAAAATTATCGTTCTTCATCGAATATCATCCTTGCGGCTTCAA
AAGTGATTTCGAACAACACTCAGAGAAAACAAAAAGAAATATTTACGAATAATCCCAAAGGTTCTCCGGTTGTGTTAAAC
GAATTTGAAAACGAATCCGAGGAAGCTCACGGAGTAATCACCAGGATCCGTTCCGCGTATTCTTACGGAACGGAATATAA
GAATATTGCAATATTCTACAGAACAAATTCTCAGTCCAGATATTTTGAGGAAGCGCTTCGGAATTTAGGAATTCCGTATA
AAATTTTCGGGGGTTTCCGATTTTTTGACAGGGCGGAAATCAAGGATTTTATCGCTTATCTGAGTGTGGTTTCCAACCCA
CTCGACAGCGTTTCACTTCTTCGAATCGTAAACTATCCTCCGAGAGGAATCGGCGATTCCGGAATTGGTAAAATCCGTGA
ATTTTCTCTGGAAAAAGGAATTTCTCTTTTGGAAGCCTTGGGCCAAGAAGATATTCCTCTCAAAAAAGCGGCGAAATCCA
AAGGGAAGGAACTCTATAGTTTATTCAGCGATCTGATAGAAAAGGTTGAAAAGAGTTCTTCCCCTTCCGAAATCGCATTA
GAACTTTTGAATCGTTCCGGTCTCGTTTCCCATTTAAGGGACGAAGGTACGGAAGAATCGATTGCCCGTTTAGAAAACCT
TCAGGAACTTGTTAATTCGATCGAGGAATACGAAAAAAATTCCGATTCTCCTTCCTTGGAAGAATATTTGAATCAGATCA
GCTTAATAACAAGTGAAGAAGAATCAAAAGAACTTACCGATTATGTGAATCTTATGACGGTACACAATGCAAAAGGTCTG
GAATTTAAGATCGTCTTTCTTTCAGGTTTGGAAGAAGGTACATTCCCTCACAGTATGAGTTTGGAAGATTCTCACTTTGG
AGACGAAGAAGAAAGAAGACTTTTTTACGTCGCTCTTACGCGTGCTCGAGAACAATTGTTTCTAAGTTATTGTAGGACTT
CCAGAAAGTTTGGTAAGGTGGAGGACAGAATTCCCTCTCGATTTCTGTCGGAAATTCCTGAAGAATGTTTCGTAGAACGT
GTAAGCCGGGAACATACTGCGAGAAGCCCCCAGGGAGCACCTTTGGCTTCTAAGACTAGGCGAATCGAAGAAAAATTTAA
TAGCGGCTTTTCATCTACACCCGATCCTACTAATCTCTATCTAAAGCCGGGAGACCGGGTAAGACACAAACAATTCGGAA
TCGGGACAATTCAGACGATTTCGGGAAAAGAAAAAAATACGAAAGTTTCCATTCGTTTCGGAAACGTTGAGAAAAACTTT
TTTGTTGCCTATACCCCACTCGAGAAATTATAA

Upstream 100 bases:

>100_bases
CTCAGAAATTGAAGTATTCGGGAGTTCCGGATCATCCCTTTACGAAATTTGAATCCGACAATGCATTTCAGTTTGTGTTC
GGAATCGGAGATTATAGATT

Downstream 100 bases:

>100_bases
GGAAGGGACTAAGTTTGCCCATTCTAAAATCCGATATTAGTATCGGAGATAGGAAACCAGATGAAGAAAATATTCGCAAT
CGCTCTTTTACATCTCATAA

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 730; Mature: 729

Protein sequence:

>730_residues
MTSSFLIGLNEEQKKAVLQITGPVLILAGAGSGKTRVITHRIANLLVNHGIDRICALTFTNKAAAEMVERVKNLVPFLPT
NLQIKTFHSLCLYILRREAFFFGFDSAFTVYDTTLQESLLKQVVKDLSLDPKFYKPSMLGNYISGLKDKMLSPETYLEKE
GRTDFSKTVSAIYKEYEKRKDANRAFDFGDLIWKTVQLFQKSPDAIAKYRHKWEYVMVDEYQDTNKVQYQLVLLLAGEKR
NLCVVGDDDQSIYSWRGADIGNILNFEKDFPESTVIKLEENYRSSSNIILAASKVISNNTQRKQKEIFTNNPKGSPVVLN
EFENESEEAHGVITRIRSAYSYGTEYKNIAIFYRTNSQSRYFEEALRNLGIPYKIFGGFRFFDRAEIKDFIAYLSVVSNP
LDSVSLLRIVNYPPRGIGDSGIGKIREFSLEKGISLLEALGQEDIPLKKAAKSKGKELYSLFSDLIEKVEKSSSPSEIAL
ELLNRSGLVSHLRDEGTEESIARLENLQELVNSIEEYEKNSDSPSLEEYLNQISLITSEEESKELTDYVNLMTVHNAKGL
EFKIVFLSGLEEGTFPHSMSLEDSHFGDEEERRLFYVALTRAREQLFLSYCRTSRKFGKVEDRIPSRFLSEIPEECFVER
VSREHTARSPQGAPLASKTRRIEEKFNSGFSSTPDPTNLYLKPGDRVRHKQFGIGTIQTISGKEKNTKVSIRFGNVEKNF
FVAYTPLEKL

Sequences:

>Translated_730_residues
MTSSFLIGLNEEQKKAVLQITGPVLILAGAGSGKTRVITHRIANLLVNHGIDRICALTFTNKAAAEMVERVKNLVPFLPT
NLQIKTFHSLCLYILRREAFFFGFDSAFTVYDTTLQESLLKQVVKDLSLDPKFYKPSMLGNYISGLKDKMLSPETYLEKE
GRTDFSKTVSAIYKEYEKRKDANRAFDFGDLIWKTVQLFQKSPDAIAKYRHKWEYVMVDEYQDTNKVQYQLVLLLAGEKR
NLCVVGDDDQSIYSWRGADIGNILNFEKDFPESTVIKLEENYRSSSNIILAASKVISNNTQRKQKEIFTNNPKGSPVVLN
EFENESEEAHGVITRIRSAYSYGTEYKNIAIFYRTNSQSRYFEEALRNLGIPYKIFGGFRFFDRAEIKDFIAYLSVVSNP
LDSVSLLRIVNYPPRGIGDSGIGKIREFSLEKGISLLEALGQEDIPLKKAAKSKGKELYSLFSDLIEKVEKSSSPSEIAL
ELLNRSGLVSHLRDEGTEESIARLENLQELVNSIEEYEKNSDSPSLEEYLNQISLITSEEESKELTDYVNLMTVHNAKGL
EFKIVFLSGLEEGTFPHSMSLEDSHFGDEEERRLFYVALTRAREQLFLSYCRTSRKFGKVEDRIPSRFLSEIPEECFVER
VSREHTARSPQGAPLASKTRRIEEKFNSGFSSTPDPTNLYLKPGDRVRHKQFGIGTIQTISGKEKNTKVSIRFGNVEKNF
FVAYTPLEKL
>Mature_729_residues
TSSFLIGLNEEQKKAVLQITGPVLILAGAGSGKTRVITHRIANLLVNHGIDRICALTFTNKAAAEMVERVKNLVPFLPTN
LQIKTFHSLCLYILRREAFFFGFDSAFTVYDTTLQESLLKQVVKDLSLDPKFYKPSMLGNYISGLKDKMLSPETYLEKEG
RTDFSKTVSAIYKEYEKRKDANRAFDFGDLIWKTVQLFQKSPDAIAKYRHKWEYVMVDEYQDTNKVQYQLVLLLAGEKRN
LCVVGDDDQSIYSWRGADIGNILNFEKDFPESTVIKLEENYRSSSNIILAASKVISNNTQRKQKEIFTNNPKGSPVVLNE
FENESEEAHGVITRIRSAYSYGTEYKNIAIFYRTNSQSRYFEEALRNLGIPYKIFGGFRFFDRAEIKDFIAYLSVVSNPL
DSVSLLRIVNYPPRGIGDSGIGKIREFSLEKGISLLEALGQEDIPLKKAAKSKGKELYSLFSDLIEKVEKSSSPSEIALE
LLNRSGLVSHLRDEGTEESIARLENLQELVNSIEEYEKNSDSPSLEEYLNQISLITSEEESKELTDYVNLMTVHNAKGLE
FKIVFLSGLEEGTFPHSMSLEDSHFGDEEERRLFYVALTRAREQLFLSYCRTSRKFGKVEDRIPSRFLSEIPEECFVERV
SREHTARSPQGAPLASKTRRIEEKFNSGFSSTPDPTNLYLKPGDRVRHKQFGIGTIQTISGKEKNTKVSIRFGNVEKNFF
VAYTPLEKL

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=745, Percent_Identity=37.5838926174497, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI48994965, Length=644, Percent_Identity=38.8198757763975, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1787196, Length=361, Percent_Identity=27.9778393351801, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=757, Percent_Identity=27.6089828269485, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6324477, Length=652, Percent_Identity=23.3128834355828, Blast_Score=114, Evalue=6e-26,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83183; Mature: 83052

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSFLIGLNEEQKKAVLQITGPVLILAGAGSGKTRVITHRIANLLVNHGIDRICALTFT
CCCCEEEECCCHHHCEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
NKAAAEMVERVKNLVPFLPTNLQIKTFHSLCLYILRREAFFFGFDSAFTVYDTTLQESLL
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHHHHHH
KQVVKDLSLDPKFYKPSMLGNYISGLKDKMLSPETYLEKEGRTDFSKTVSAIYKEYEKRK
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
DANRAFDFGDLIWKTVQLFQKSPDAIAKYRHKWEYVMVDEYQDTNKVQYQLVLLLAGEKR
HHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEECCCC
NLCVVGDDDQSIYSWRGADIGNILNFEKDFPESTVIKLEENYRSSSNIILAASKVISNNT
CEEEECCCCCCHHEECCCCHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEHHHHCCCH
QRKQKEIFTNNPKGSPVVLNEFENESEEAHGVITRIRSAYSYGTEYKNIAIFYRTNSQSR
HHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHH
YFEEALRNLGIPYKIFGGFRFFDRAEIKDFIAYLSVVSNPLDSVSLLRIVNYPPRGIGDS
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
GIGKIREFSLEKGISLLEALGQEDIPLKKAAKSKGKELYSLFSDLIEKVEKSSSPSEIAL
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ELLNRSGLVSHLRDEGTEESIARLENLQELVNSIEEYEKNSDSPSLEEYLNQISLITSEE
HHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
ESKELTDYVNLMTVHNAKGLEFKIVFLSGLEEGTFPHSMSLEDSHFGDEEERRLFYVALT
HHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHEEEEHHHH
RAREQLFLSYCRTSRKFGKVEDRIPSRFLSEIPEECFVERVSREHTARSPQGAPLASKTR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
RIEEKFNSGFSSTPDPTNLYLKPGDRVRHKQFGIGTIQTISGKEKNTKVSIRFGNVEKNF
HHHHHHCCCCCCCCCCCEEEECCCCHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCE
FVAYTPLEKL
EEEECCHHCC
>Mature Secondary Structure 
TSSFLIGLNEEQKKAVLQITGPVLILAGAGSGKTRVITHRIANLLVNHGIDRICALTFT
CCCEEEECCCHHHCEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
NKAAAEMVERVKNLVPFLPTNLQIKTFHSLCLYILRREAFFFGFDSAFTVYDTTLQESLL
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEHHHHHHHHHH
KQVVKDLSLDPKFYKPSMLGNYISGLKDKMLSPETYLEKEGRTDFSKTVSAIYKEYEKRK
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
DANRAFDFGDLIWKTVQLFQKSPDAIAKYRHKWEYVMVDEYQDTNKVQYQLVLLLAGEKR
HHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEECCCC
NLCVVGDDDQSIYSWRGADIGNILNFEKDFPESTVIKLEENYRSSSNIILAASKVISNNT
CEEEECCCCCCHHEECCCCHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEHHHHCCCH
QRKQKEIFTNNPKGSPVVLNEFENESEEAHGVITRIRSAYSYGTEYKNIAIFYRTNSQSR
HHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHH
YFEEALRNLGIPYKIFGGFRFFDRAEIKDFIAYLSVVSNPLDSVSLLRIVNYPPRGIGDS
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
GIGKIREFSLEKGISLLEALGQEDIPLKKAAKSKGKELYSLFSDLIEKVEKSSSPSEIAL
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ELLNRSGLVSHLRDEGTEESIARLENLQELVNSIEEYEKNSDSPSLEEYLNQISLITSEE
HHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
ESKELTDYVNLMTVHNAKGLEFKIVFLSGLEEGTFPHSMSLEDSHFGDEEERRLFYVALT
HHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHEEEEHHHH
RAREQLFLSYCRTSRKFGKVEDRIPSRFLSEIPEECFVERVSREHTARSPQGAPLASKTR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
RIEEKFNSGFSSTPDPTNLYLKPGDRVRHKQFGIGTIQTISGKEKNTKVSIRFGNVEKNF
HHHHHHCCCCCCCCCCCEEEECCCCHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCE
FVAYTPLEKL
EEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]