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The map label for this gene is leuA-3 [H]

Identifier: 116328181

GI number: 116328181

Start: 1762786

End: 1764336

Strand: Direct

Name: leuA-3 [H]

Synonym: LBL_1505

Alternate gene names: 116328181

Gene position: 1762786-1764336 (Clockwise)

Preceding gene: 116328180

Following gene: 116328182

Centisome position: 48.77

GC content: 43.78

Gene sequence:

>1551_bases
ATGACAAAAGCAGAAACTCGATTGGAAATTTTAGACGTAACCTTGAGAGACGGAGAACAAACCAGAGGCGTTAGTTTTTC
GACTTCCGAAAAACTGAATATTGCCAAATTCTTATTGCAAAAACTCAACGTGGATCGGGTGGAAATTGCTTCTGCAAGAG
TTTCCAAAGGAGAATTGGAAACGGTTCAAAAAATCATAGAATGGGCTGAAACGGAACAACTCACGGAAAGAATCGAAATT
TTAGGATTTGTGGACGGAAACAAGACCGTAGATTGGATTAAAGATAGTGGCGCAAGGGTTTTGAATCTCCTCACAAAAGG
TTCTCTGCATCATCTCGAGAAACAGTTAAACAAAACTCCGAAAGAATTTTTTGCAGACGTTTCCATTGTCATAGAATACG
CAATCAAAAATGGCCTTAAGATAAATGTATACCTCGAAGATTGGTCCAACGGATTTAGAAACAGTCCCGATTATGTCGAA
GCGCTTGTGAAACATCTAAGCAAGGAACGTATCGAAAGAATTTTTCTTCCTGATACGTTAGGTGTTCTTTCTCCCGAAGA
AACGTTTCAAGGAGTGGACAGTCTCGTTCAAAAGTATCCGAATCTTCATTTCGAATTTCATGGACACAACGACTACGACC
TTTCCGTGGCAAACAGCCTTCAGGCAATCCGGGCAGGGGTCAAAGGTCTCCACGCGTCCATGAACGGGCTCGGAGAAAGA
GCGGGTAATACTCCACTCGAGGCGCTTGTCACCACAATCCATGATAAGTCTCAGGCCAAGACGAACATAAATGAATTGGC
CATTACGGAAGCAAGTCGCCTTGTGGAAGTGTTCAGCGGAAAAAGGATCTCTGCAAACAGACCGATCGTAGGAGAGGACG
TGTTTACTCAAACCGCGGGAGTTCATGCAGACGGAGACAAAAAAGGAAACTTGTACGCAAATCCGATTCTACCGGAGCGG
TTCGGTAGAAAAAGAAGTTACGCGTTAGGTAAGCTTTCTGGCAAAGCGAGCATCTCCGAAAACGTAAAACAACTGGGAAT
GGTGTTAAGCGACATTGTTCTTCAAAAAGTTTTAGAGAGGGTGATCGAACTCGGAGACCAGAATAAACTCGTGACTCCTG
AAGACCTTCCGTTTATCATCGCGGACGTATCCGGAAGGACCGGAGAAAAGGTTCTAACAATCAAATCTTGTAATATTCAT
TCCGGGATCGGAGTTCGGCCTCACGCTCAGATCGAATTGGAATACCAAGGAAAGGTTCATAAAGAAATTTCCGAAGGGGA
CGGAGGTTACGACGCATTTATGAATGCGCTCACAAAAATTACCAATCGCCTCGGAATCAGCATTCCTAAGTTGATAGATT
ACGAAGTTCGAATTCCTCCCGGAGGAAAAACGGATGCTCTCGTAGAAACTATGATCACTTGGAACAAGTCTTTGGATTTG
GAAGAAGATCAGACTTTCAAAACAATGGGGGTTCATCCGGATCAAACGATTGCGGCCGTTCACGCAACTGAAAAAATGCT
CAATCAGATCCTGCAACCATGGCAAACTTAA

Upstream 100 bases:

>100_bases
TAAAAGAGCTCGACTATACTCAATTTGGAATTTCCAATCCGGGTTCTACGTTGGTCGAAGTATTAGGTCCCGATAAGGAC
ACTCTCAAAAGGTATCTCAG

Downstream 100 bases:

>100_bases
AACTTTTACTTGTGGGTATTGGAAACCCTGGCCCAAAATATGCCTATAATCGGCATAACATCGGTTTTGTGATTTTAGAT
TCTCTTTTAAATTCTTCTTC

Product: 2-isopropylmalate/homocitrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTKAETRLEILDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELETVQKIIEWAETEQLTERIEI
LGFVDGNKTVDWIKDSGARVLNLLTKGSLHHLEKQLNKTPKEFFADVSIVIEYAIKNGLKINVYLEDWSNGFRNSPDYVE
ALVKHLSKERIERIFLPDTLGVLSPEETFQGVDSLVQKYPNLHFEFHGHNDYDLSVANSLQAIRAGVKGLHASMNGLGER
AGNTPLEALVTTIHDKSQAKTNINELAITEASRLVEVFSGKRISANRPIVGEDVFTQTAGVHADGDKKGNLYANPILPER
FGRKRSYALGKLSGKASISENVKQLGMVLSDIVLQKVLERVIELGDQNKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIH
SGIGVRPHAQIELEYQGKVHKEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETMITWNKSLDL
EEDQTFKTMGVHPDQTIAAVHATEKMLNQILQPWQT

Sequences:

>Translated_516_residues
MTKAETRLEILDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELETVQKIIEWAETEQLTERIEI
LGFVDGNKTVDWIKDSGARVLNLLTKGSLHHLEKQLNKTPKEFFADVSIVIEYAIKNGLKINVYLEDWSNGFRNSPDYVE
ALVKHLSKERIERIFLPDTLGVLSPEETFQGVDSLVQKYPNLHFEFHGHNDYDLSVANSLQAIRAGVKGLHASMNGLGER
AGNTPLEALVTTIHDKSQAKTNINELAITEASRLVEVFSGKRISANRPIVGEDVFTQTAGVHADGDKKGNLYANPILPER
FGRKRSYALGKLSGKASISENVKQLGMVLSDIVLQKVLERVIELGDQNKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIH
SGIGVRPHAQIELEYQGKVHKEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETMITWNKSLDL
EEDQTFKTMGVHPDQTIAAVHATEKMLNQILQPWQT
>Mature_515_residues
TKAETRLEILDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELETVQKIIEWAETEQLTERIEIL
GFVDGNKTVDWIKDSGARVLNLLTKGSLHHLEKQLNKTPKEFFADVSIVIEYAIKNGLKINVYLEDWSNGFRNSPDYVEA
LVKHLSKERIERIFLPDTLGVLSPEETFQGVDSLVQKYPNLHFEFHGHNDYDLSVANSLQAIRAGVKGLHASMNGLGERA
GNTPLEALVTTIHDKSQAKTNINELAITEASRLVEVFSGKRISANRPIVGEDVFTQTAGVHADGDKKGNLYANPILPERF
GRKRSYALGKLSGKASISENVKQLGMVLSDIVLQKVLERVIELGDQNKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIHS
GIGVRPHAQIELEYQGKVHKEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETMITWNKSLDLE
EDQTFKTMGVHPDQTIAAVHATEKMLNQILQPWQT

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=513, Percent_Identity=27.8752436647173, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320071, Length=398, Percent_Identity=28.391959798995, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320019, Length=376, Percent_Identity=27.3936170212766, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 57349; Mature: 57218

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAETRLEILDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELE
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHCCCHHHH
TVQKIIEWAETEQLTERIEILGFVDGNKTVDWIKDSGARVLNLLTKGSLHHLEKQLNKTP
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHCCCH
KEFFADVSIVIEYAIKNGLKINVYLEDWSNGFRNSPDYVEALVKHLSKERIERIFLPDTL
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
GVLSPEETFQGVDSLVQKYPNLHFEFHGHNDYDLSVANSLQAIRAGVKGLHASMNGLGER
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGNTPLEALVTTIHDKSQAKTNINELAITEASRLVEVFSGKRISANRPIVGEDVFTQTAG
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHCCC
VHADGDKKGNLYANPILPERFGRKRSYALGKLSGKASISENVKQLGMVLSDIVLQKVLER
CCCCCCCCCCEEECCCCHHHHCCCHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VIELGDQNKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIHSGIGVRPHAQIELEYQGKVH
HHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHH
KEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETMITWNKSLDL
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCC
EEDQTFKTMGVHPDQTIAAVHATEKMLNQILQPWQT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKAETRLEILDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELE
CCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHCCCHHHH
TVQKIIEWAETEQLTERIEILGFVDGNKTVDWIKDSGARVLNLLTKGSLHHLEKQLNKTP
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHCCCH
KEFFADVSIVIEYAIKNGLKINVYLEDWSNGFRNSPDYVEALVKHLSKERIERIFLPDTL
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
GVLSPEETFQGVDSLVQKYPNLHFEFHGHNDYDLSVANSLQAIRAGVKGLHASMNGLGER
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGNTPLEALVTTIHDKSQAKTNINELAITEASRLVEVFSGKRISANRPIVGEDVFTQTAG
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHCCC
VHADGDKKGNLYANPILPERFGRKRSYALGKLSGKASISENVKQLGMVLSDIVLQKVLER
CCCCCCCCCCEEECCCCHHHHCCCHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VIELGDQNKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIHSGIGVRPHAQIELEYQGKVH
HHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHH
KEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETMITWNKSLDL
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCC
EEDQTFKTMGVHPDQTIAAVHATEKMLNQILQPWQT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]