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The map label for this gene is leuC [H]

Identifier: 116328074

GI number: 116328074

Start: 1602774

End: 1604171

Strand: Direct

Name: leuC [H]

Synonym: LBL_1371

Alternate gene names: 116328074

Gene position: 1602774-1604171 (Clockwise)

Preceding gene: 116328073

Following gene: 116328075

Centisome position: 44.34

GC content: 48.35

Gene sequence:

>1398_bases
ATGAAGACAATGTTCGAAAAAATCTGGGAAGACCATCTGGTTGGAGAACTGGATGCGGGATCGTATTTAATTTATATCGA
TCGTCATCTCATTCACGAAGTTACAAGCCCCCAGGCTTTCGACGGACTCAAACTCGCGGGTAGAAAGGTCCGTCGTCCCG
AAGCGACGTTTGCTACTATGGATCATAACGTTTCCACTCGCACTCGGGATCTCAGTCTCGCCGATCCGATTTCGGCGATT
CAGATGCAGACTTTGAAGAAGAATTGCGATGAAAACGGAATCCGCCTTTATGACTTTCAAAATCCCGATCAAGGGATTAT
CCACGTAATCGCTCCCGAAATGGGTCTGACTCATCCTGGAATGACGATCGTCTGCGGGGATTCTCATACTTCTACTCACG
GCGCTTTTGGCGCGCTTGCGTTCGGGATTGGAACCAGCGAAGTGGAACACGTTCTCGCGACTCAAACTCTCGTTCAAAAA
AAAGCGAAGACTATGGAAATTCGAGTCGACGGAAAACTTTCCGACAAGGTGACCGCGAAAGACATTATACTTGCGATTAT
CGGTAAAATCGGCACTGCGGGGGCAACCGGTTACGTAATCGAGTACCGAGGTTCTGCGATTCAAGCGCTCAGCATGGAAG
CGAGAATGACCGTTTGTAATATGTCGATCGAAGCGGGAGCGAGAGCGGGACTGATTGCTCCGGACGAAATCACGTTCAAT
TATATCAAAGGAAAAGATTTCGCACCGAAAGGCGCCCAATGGGATCTTGCCGTTAAAAAGTGGAAACACTATGTAACCGA
CGAAGGTGCAAAGTTTGACACCACCGTAATTCTTCATGCAGATGAAATTACCCCGATGGTGACTTGGGGAACTTCTCCCA
GTCAAGTTGTTTCCGTAAAAGGAACCGTTCCAAGTCCGGAGGATGCAACAGATCCAATCGATAAGATTGGAATTTCATCC
GCTCTTAAATATATGGACTTGAAACCCGGGGAAAAGATCGAAGAAATCAAAATCAACAAAGTTTTTATCGGTTCCTGCAC
CAATTCCAGAATCGAAGATCTGAGGGCTGCGGCTACAACTATCCAAGGAAAAAAAGTTTCTTCCAAGGTTCAGGCGATCG
TGGTTCCGGGTTCGGGGAGAGTCAAACGCCAAGCGGAACAAGAAGGTCTGGATAAGATCTTTATCGCGGCCGGCTTTGAG
TGGAGAAACCCAGGTTGTTCCATGTGTCTTGCAATGAACGACGACGTTCTCGAACCGGGAGATCGTTGCGCTTCCACTTC
CAATAGAAACTTTGAAGGCCGCCAAGGAAAGGGTGGAAGAACTCATCTCGTCGGGCCGGAAATGGCGGCAGCGGCAGCGG
TGGAAGGGCATTTTGTGGATATCCGAAGTTGGAAATAA

Upstream 100 bases:

>100_bases
CCTGACGATGGAAATTCAGGCGGATATCTTTTCTCTTTTCGAGAAACAAATCCAATCCACAAATTCCGAAAGAATTTCTT
TCGGGGAGTACTAGAATCCA

Downstream 100 bases:

>100_bases
TCAAGAGGCAAATTAGAATATTATGAAGCCATTTACTGTATTGAACGGGATTGCCGCGTTACTCGATAGACCGAACGTCG
ACACGGATCAGATCATTCCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKTMFEKIWEDHLVGELDAGSYLIYIDRHLIHEVTSPQAFDGLKLAGRKVRRPEATFATMDHNVSTRTRDLSLADPISAI
QMQTLKKNCDENGIRLYDFQNPDQGIIHVIAPEMGLTHPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQK
KAKTMEIRVDGKLSDKVTAKDIILAIIGKIGTAGATGYVIEYRGSAIQALSMEARMTVCNMSIEAGARAGLIAPDEITFN
YIKGKDFAPKGAQWDLAVKKWKHYVTDEGAKFDTTVILHADEITPMVTWGTSPSQVVSVKGTVPSPEDATDPIDKIGISS
ALKYMDLKPGEKIEEIKINKVFIGSCTNSRIEDLRAAATTIQGKKVSSKVQAIVVPGSGRVKRQAEQEGLDKIFIAAGFE
WRNPGCSMCLAMNDDVLEPGDRCASTSNRNFEGRQGKGGRTHLVGPEMAAAAAVEGHFVDIRSWK

Sequences:

>Translated_465_residues
MKTMFEKIWEDHLVGELDAGSYLIYIDRHLIHEVTSPQAFDGLKLAGRKVRRPEATFATMDHNVSTRTRDLSLADPISAI
QMQTLKKNCDENGIRLYDFQNPDQGIIHVIAPEMGLTHPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQK
KAKTMEIRVDGKLSDKVTAKDIILAIIGKIGTAGATGYVIEYRGSAIQALSMEARMTVCNMSIEAGARAGLIAPDEITFN
YIKGKDFAPKGAQWDLAVKKWKHYVTDEGAKFDTTVILHADEITPMVTWGTSPSQVVSVKGTVPSPEDATDPIDKIGISS
ALKYMDLKPGEKIEEIKINKVFIGSCTNSRIEDLRAAATTIQGKKVSSKVQAIVVPGSGRVKRQAEQEGLDKIFIAAGFE
WRNPGCSMCLAMNDDVLEPGDRCASTSNRNFEGRQGKGGRTHLVGPEMAAAAAVEGHFVDIRSWK
>Mature_465_residues
MKTMFEKIWEDHLVGELDAGSYLIYIDRHLIHEVTSPQAFDGLKLAGRKVRRPEATFATMDHNVSTRTRDLSLADPISAI
QMQTLKKNCDENGIRLYDFQNPDQGIIHVIAPEMGLTHPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQK
KAKTMEIRVDGKLSDKVTAKDIILAIIGKIGTAGATGYVIEYRGSAIQALSMEARMTVCNMSIEAGARAGLIAPDEITFN
YIKGKDFAPKGAQWDLAVKKWKHYVTDEGAKFDTTVILHADEITPMVTWGTSPSQVVSVKGTVPSPEDATDPIDKIGISS
ALKYMDLKPGEKIEEIKINKVFIGSCTNSRIEDLRAAATTIQGKKVSSKVQAIVVPGSGRVKRQAEQEGLDKIFIAAGFE
WRNPGCSMCLAMNDDVLEPGDRCASTSNRNFEGRQGKGGRTHLVGPEMAAAAAVEGHFVDIRSWK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=368, Percent_Identity=27.445652173913, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI8659555, Length=410, Percent_Identity=25.8536585365854, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI41352693, Length=380, Percent_Identity=24.7368421052632, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=66.8103448275862, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=23.8227146814404, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI2367097, Length=356, Percent_Identity=24.438202247191, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=372, Percent_Identity=30.6451612903226, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=372, Percent_Identity=30.6451612903226, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=311, Percent_Identity=29.903536977492, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=409, Percent_Identity=25.9168704156479, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=59.2356687898089, Blast_Score=575, Evalue=1e-165,
Organism=Saccharomyces cerevisiae, GI6320440, Length=435, Percent_Identity=25.9770114942529, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=369, Percent_Identity=27.6422764227642, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322261, Length=368, Percent_Identity=27.1739130434783, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28571643, Length=474, Percent_Identity=28.2700421940928, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI281365315, Length=477, Percent_Identity=27.0440251572327, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17864292, Length=477, Percent_Identity=27.0440251572327, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI161076999, Length=401, Percent_Identity=27.6807980049875, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24645686, Length=374, Percent_Identity=26.7379679144385, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137564, Length=372, Percent_Identity=25.8064516129032, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50543; Mature: 50543

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMFEKIWEDHLVGELDAGSYLIYIDRHLIHEVTSPQAFDGLKLAGRKVRRPEATFATM
CCHHHHHHHHHHCCEEECCCCEEEEEEHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEEEE
DHNVSTRTRDLSLADPISAIQMQTLKKNCDENGIRLYDFQNPDQGIIHVIAPEMGLTHPG
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCC
MTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQKKAKTMEIRVDGKLSDKVTAK
CEEEECCCCCCCCCCHHHEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHH
DIILAIIGKIGTAGATGYVIEYRGSAIQALSMEARMTVCNMSIEAGARAGLIAPDEITFN
HHHHHHHHCCCCCCCCEEEEEECCCEEEEEEHHEEEEEEEEEHHCCCCCCCCCCCCEEEE
YIKGKDFAPKGAQWDLAVKKWKHYVTDEGAKFDTTVILHADEITPMVTWGTSPSQVVSVK
EEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCCEEEEEE
GTVPSPEDATDPIDKIGISSALKYMDLKPGEKIEEIKINKVFIGSCTNSRIEDLRAAATT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHH
IQGKKVSSKVQAIVVPGSGRVKRQAEQEGLDKIFIAAGFEWRNPGCSMCLAMNDDVLEPG
CCCCEECCCEEEEEECCCCCHHHHHHHCCCCEEEEEECCEECCCCCCEEEEECCCCCCCC
DRCASTSNRNFEGRQGKGGRTHLVGPEMAAAAAVEGHFVDIRSWK
HHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCEEEEEECCC
>Mature Secondary Structure
MKTMFEKIWEDHLVGELDAGSYLIYIDRHLIHEVTSPQAFDGLKLAGRKVRRPEATFATM
CCHHHHHHHHHHCCEEECCCCEEEEEEHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEEEE
DHNVSTRTRDLSLADPISAIQMQTLKKNCDENGIRLYDFQNPDQGIIHVIAPEMGLTHPG
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCC
MTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQKKAKTMEIRVDGKLSDKVTAK
CEEEECCCCCCCCCCHHHEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHH
DIILAIIGKIGTAGATGYVIEYRGSAIQALSMEARMTVCNMSIEAGARAGLIAPDEITFN
HHHHHHHHCCCCCCCCEEEEEECCCEEEEEEHHEEEEEEEEEHHCCCCCCCCCCCCEEEE
YIKGKDFAPKGAQWDLAVKKWKHYVTDEGAKFDTTVILHADEITPMVTWGTSPSQVVSVK
EEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCCEEEEEE
GTVPSPEDATDPIDKIGISSALKYMDLKPGEKIEEIKINKVFIGSCTNSRIEDLRAAATT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHH
IQGKKVSSKVQAIVVPGSGRVKRQAEQEGLDKIFIAAGFEWRNPGCSMCLAMNDDVLEPG
CCCCEECCCEEEEEECCCCCHHHHHHHCCCCEEEEEECCEECCCCCCEEEEECCCCCCCC
DRCASTSNRNFEGRQGKGGRTHLVGPEMAAAAAVEGHFVDIRSWK
HHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA