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The map label for this gene is rsmE [H]

Identifier: 116328047

GI number: 116328047

Start: 1576397

End: 1577179

Strand: Reverse

Name: rsmE [H]

Synonym: LBL_1342

Alternate gene names: 116328047

Gene position: 1577179-1576397 (Counterclockwise)

Preceding gene: 116328048

Following gene: 116328045

Centisome position: 43.64

GC content: 40.36

Gene sequence:

>783_bases
TTGAATCTACTGATCTGCAAAGAAGAATGGAGAATCGCGAAATCGAACCGATTTTCCATTCAGGATCCGCAAAAGATCCA
ACACATATGCAATATTTTGAATAAACGAGAAGGGGCCCGTTTAAAAGCGGGGCTCATAGACACTAGCCTCGGAAAATTTA
TCCTAGAAAAAATCGAGTCGAATCGAATCGTCGGAATCTACAAACCGATTTTAGTTCCGATCCCTCGTTTCCCTGAAGTT
CATATTCTACTCGCGATCAATCGACCTCCCACCGTGAGAAAAATTCTTCAGTTGGCCGGAACCTGGGGAGTTAGTTCCAT
TCATTTTTTTGTCAGTAGAAATTCCAGGAGAGAATATCTTACTTCTCCCGTTTGGAATTCGACCGAAATCGAATCGGAGC
TTCTGGAAGGAATGGAACAAGGGAAAAATATCTTTCTTCCAAAAATCCATTTCGATTTTAAAAATCGATCGGAAACAATT
CTTCTGGAGAAATTTAAAAAGGTAGATTTTACATTTCGTTTCGTTTTGGATCGACGAGGGGCTACACTTCCACAAATTAT
CGAAGAAAAAAAATCGGATTCAAATTTCGGAATGCCTTCTAAAACACCGCAAGTCTTAGTAGCAATCGGTCCGGAAAGCG
GCTTTGTTCGAAACGAAATCGATTTTTGGAAACGTTTCGGCTTTAAAAAGTTGAACCTTTCCAGCCGTGTCTTACGCACC
GAAACAGCCGTTGCGTTCCTTCTTTCAAGACTCGAAGAGAAAGATCTTTTCTTAAAAACTTAA

Upstream 100 bases:

>100_bases
CCCAAAAACTCGACCGCGGGAAATCATTCCGGAAGAGCCTTCCCCGCCACCTCAGGAACAACCGGAACCCAAATTTGAAA
TCTAATCCCGCTTTTGCCTC

Downstream 100 bases:

>100_bases
ACCGCCCGAGAACATTACCTTCTGTTCATCCTGAGCTTCGTATTGTCCGGATGCGGTATCATAAACCCATTTACGAATAT
TCTGAAGTCTTACGGTGATA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNLLICKEEWRIAKSNRFSIQDPQKIQHICNILNKREGARLKAGLIDTSLGKFILEKIESNRIVGIYKPILVPIPRFPEV
HILLAINRPPTVRKILQLAGTWGVSSIHFFVSRNSRREYLTSPVWNSTEIESELLEGMEQGKNIFLPKIHFDFKNRSETI
LLEKFKKVDFTFRFVLDRRGATLPQIIEEKKSDSNFGMPSKTPQVLVAIGPESGFVRNEIDFWKRFGFKKLNLSSRVLRT
ETAVAFLLSRLEEKDLFLKT

Sequences:

>Translated_260_residues
MNLLICKEEWRIAKSNRFSIQDPQKIQHICNILNKREGARLKAGLIDTSLGKFILEKIESNRIVGIYKPILVPIPRFPEV
HILLAINRPPTVRKILQLAGTWGVSSIHFFVSRNSRREYLTSPVWNSTEIESELLEGMEQGKNIFLPKIHFDFKNRSETI
LLEKFKKVDFTFRFVLDRRGATLPQIIEEKKSDSNFGMPSKTPQVLVAIGPESGFVRNEIDFWKRFGFKKLNLSSRVLRT
ETAVAFLLSRLEEKDLFLKT
>Mature_260_residues
MNLLICKEEWRIAKSNRFSIQDPQKIQHICNILNKREGARLKAGLIDTSLGKFILEKIESNRIVGIYKPILVPIPRFPEV
HILLAINRPPTVRKILQLAGTWGVSSIHFFVSRNSRREYLTSPVWNSTEIESELLEGMEQGKNIFLPKIHFDFKNRSETI
LLEKFKKVDFTFRFVLDRRGATLPQIIEEKKSDSNFGMPSKTPQVLVAIGPESGFVRNEIDFWKRFGFKKLNLSSRVLRT
ETAVAFLLSRLEEKDLFLKT

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 30129; Mature: 30129

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLICKEEWRIAKSNRFSIQDPQKIQHICNILNKREGARLKAGLIDTSLGKFILEKIES
CCEEEECHHCEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCC
NRIVGIYKPILVPIPRFPEVHILLAINRPPTVRKILQLAGTWGVSSIHFFVSRNSRREYL
CCEEEEECHHEECCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHH
TSPVWNSTEIESELLEGMEQGKNIFLPKIHFDFKNRSETILLEKFKKVDFTFRFVLDRRG
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCEEEEEEEECCC
ATLPQIIEEKKSDSNFGMPSKTPQVLVAIGPESGFVRNEIDFWKRFGFKKLNLSSRVLRT
CHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEECCHHHHHHH
ETAVAFLLSRLEEKDLFLKT
HHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MNLLICKEEWRIAKSNRFSIQDPQKIQHICNILNKREGARLKAGLIDTSLGKFILEKIES
CCEEEECHHCEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCC
NRIVGIYKPILVPIPRFPEVHILLAINRPPTVRKILQLAGTWGVSSIHFFVSRNSRREYL
CCEEEEECHHEECCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHH
TSPVWNSTEIESELLEGMEQGKNIFLPKIHFDFKNRSETILLEKFKKVDFTFRFVLDRRG
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCEEEEEEEECCC
ATLPQIIEEKKSDSNFGMPSKTPQVLVAIGPESGFVRNEIDFWKRFGFKKLNLSSRVLRT
CHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEECCHHHHHHH
ETAVAFLLSRLEEKDLFLKT
HHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]