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The map label for this gene is garR [C]

Identifier: 116328038

GI number: 116328038

Start: 1566657

End: 1567544

Strand: Reverse

Name: garR [C]

Synonym: LBL_1333

Alternate gene names: 116328038

Gene position: 1567544-1566657 (Counterclockwise)

Preceding gene: 116328039

Following gene: 116328031

Centisome position: 43.37

GC content: 44.82

Gene sequence:

>888_bases
ATGAAGAAACATACGATATCCATCATCGGAACCGGAATCATGGGCAGAGGTATAGCGTCCAATCTCGCAAAAGCGGATCA
AAATCTCAGACTCTACACAAGAAACAAACTCAAAATCGCCGATTTGATAAGCGACAACGTTCAGATCTTCGATTCTCCCA
TGGAAGCGGTTAAAAATGCGAATATAGTCATATTATGTCTGACCGAAGACACAGTCATCGAAAAAGAAGTGATCACATCC
GGACTTTTAGAGACAAAACCTCCGATTATCCTAGACTGCGGAACCACATCCCTCTCCATGACTTTCAGACTCGCGAAACT
ATGTTCGGCAAAACAGATTCGCTTTTACGATTCTCCGATGACGGGCTCGAAGAACGCAGCGAGAGACGGACAGATTTTAT
TTATGATCGGCGCAGATCAGGCGGATGTTTCCGACATCGGCTTCTTTTTCGAGATTTGCGGTAAGAACACGGTGTATTGC
GGTCAGGTCGGCGGAGGACAGAAGGCGAAACTCGCTCTCAATATGATTCAAGCGGGAATTTTTCAAATTTATATGGAAGG
CTTCGAACTCGCAAAAAATTCAGGGGTGGAACCGGAAATCCTAAAAGACATTCTCCTGGGGTCCGCCGCAAAATCAGGGA
TCGCCGAATTTAAGTTTCCCTTTGTATTCTCCGGAAACTATGAAACTCATTTTTCTTTGAAGAATATGAGAAAAGACGTC
TATCACGCGATGGAACTCGCGAAAGAAAACAAGACGAACCTTTCCCTTTGTCAAAATCTTCCCGAGATTTACGACGCGGG
AATGAATGCCGGTTTTGGAGAAAACGACTTCTGTAGTCTAAACGAGGTCACAGCGAAGATCCGTCCTCCCAAATCAGAGA
ATCCGTAA

Upstream 100 bases:

>100_bases
TTAGGGAAGGACTCGAGGACACAAGGGAACAAGCCCCTGTCACAAGCTTTATTCGAGCTGCCTTAAGCGCTTTTTAATAT
TATAAATTACTTTTATACCT

Downstream 100 bases:

>100_bases
TCGCCTTTCCTTCGGAGTCGACTTTTAAACGAATATGAACTTTCTCTTCCCGAAGACGTTTTTCGTAGTCGAGAGCCTTG
ATTTCGTTCACATAAAAACG

Product: beta-hydroxyacid dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKKHTISIIGTGIMGRGIASNLAKADQNLRLYTRNKLKIADLISDNVQIFDSPMEAVKNANIVILCLTEDTVIEKEVITS
GLLETKPPIILDCGTTSLSMTFRLAKLCSAKQIRFYDSPMTGSKNAARDGQILFMIGADQADVSDIGFFFEICGKNTVYC
GQVGGGQKAKLALNMIQAGIFQIYMEGFELAKNSGVEPEILKDILLGSAAKSGIAEFKFPFVFSGNYETHFSLKNMRKDV
YHAMELAKENKTNLSLCQNLPEIYDAGMNAGFGENDFCSLNEVTAKIRPPKSENP

Sequences:

>Translated_295_residues
MKKHTISIIGTGIMGRGIASNLAKADQNLRLYTRNKLKIADLISDNVQIFDSPMEAVKNANIVILCLTEDTVIEKEVITS
GLLETKPPIILDCGTTSLSMTFRLAKLCSAKQIRFYDSPMTGSKNAARDGQILFMIGADQADVSDIGFFFEICGKNTVYC
GQVGGGQKAKLALNMIQAGIFQIYMEGFELAKNSGVEPEILKDILLGSAAKSGIAEFKFPFVFSGNYETHFSLKNMRKDV
YHAMELAKENKTNLSLCQNLPEIYDAGMNAGFGENDFCSLNEVTAKIRPPKSENP
>Mature_295_residues
MKKHTISIIGTGIMGRGIASNLAKADQNLRLYTRNKLKIADLISDNVQIFDSPMEAVKNANIVILCLTEDTVIEKEVITS
GLLETKPPIILDCGTTSLSMTFRLAKLCSAKQIRFYDSPMTGSKNAARDGQILFMIGADQADVSDIGFFFEICGKNTVYC
GQVGGGQKAKLALNMIQAGIFQIYMEGFELAKNSGVEPEILKDILLGSAAKSGIAEFKFPFVFSGNYETHFSLKNMRKDV
YHAMELAKENKTNLSLCQNLPEIYDAGMNAGFGENDFCSLNEVTAKIRPPKSENP

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=26.4285714285714, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI23308751, Length=288, Percent_Identity=28.4722222222222, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI145693186, Length=278, Percent_Identity=25.1798561151079, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786719, Length=264, Percent_Identity=28.4090909090909, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=23.1292517006803, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24655230, Length=291, Percent_Identity=26.8041237113402, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19922568, Length=291, Percent_Identity=26.8041237113402, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28574115, Length=279, Percent_Identity=20.4301075268817, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 32322; Mature: 32322

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHTISIIGTGIMGRGIASNLAKADQNLRLYTRNKLKIADLISDNVQIFDSPMEAVKNA
CCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCEEEEHHHCCCCEEECCHHHHHCCC
NIVILCLTEDTVIEKEVITSGLLETKPPIILDCGTTSLSMTFRLAKLCSAKQIRFYDSPM
CEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCC
TGSKNAARDGQILFMIGADQADVSDIGFFFEICGKNTVYCGQVGGGQKAKLALNMIQAGI
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
FQIYMEGFELAKNSGVEPEILKDILLGSAAKSGIAEFKFPFVFSGNYETHFSLKNMRKDV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHEECCEEEECCCEEEEHHHHHHHHH
YHAMELAKENKTNLSLCQNLPEIYDAGMNAGFGENDFCSLNEVTAKIRPPKSENP
HHHHHHHHCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHEEECCCCCCCC
>Mature Secondary Structure
MKKHTISIIGTGIMGRGIASNLAKADQNLRLYTRNKLKIADLISDNVQIFDSPMEAVKNA
CCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCEEEEHHHCCCCEEECCHHHHHCCC
NIVILCLTEDTVIEKEVITSGLLETKPPIILDCGTTSLSMTFRLAKLCSAKQIRFYDSPM
CEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCC
TGSKNAARDGQILFMIGADQADVSDIGFFFEICGKNTVYCGQVGGGQKAKLALNMIQAGI
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
FQIYMEGFELAKNSGVEPEILKDILLGSAAKSGIAEFKFPFVFSGNYETHFSLKNMRKDV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHEECCEEEECCCEEEEHHHHHHHHH
YHAMELAKENKTNLSLCQNLPEIYDAGMNAGFGENDFCSLNEVTAKIRPPKSENP
HHHHHHHHCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA