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The map label for this gene is cysK-1 [H]

Identifier: 116327868

GI number: 116327868

Start: 1358581

End: 1359561

Strand: Direct

Name: cysK-1 [H]

Synonym: LBL_1148

Alternate gene names: 116327868

Gene position: 1358581-1359561 (Clockwise)

Preceding gene: 116327867

Following gene: 116327869

Centisome position: 37.59

GC content: 44.65

Gene sequence:

>981_bases
ATGAATGTTAAAAAAGATTTTTCCGATGCGGTAGGAAATACACCGCTTATTTTGCTTCGTAGTTTCAGCGAGGAAACCGG
TTGTAACATCTATGGGAAGGCGGAGTTCTTAAATCCGGGAGGTTCCGTTAAGGATCGGGCAGCTCTTTTTATCGTGGAAG
ATTCTGAAAGAAAAGGGCTTTTGAAAGCCGGTGGAACGGTGGTGGAGGGAACCGCAGGAAATACGGGAATCGGGCTTACT
CATATTTGTAACTCGAAGGGTTATAAAACGTTAATTATCATTCCAGATACGCAGTCTAAGGAGAAAATCGATCTCTTACG
GACCTTGGGTGCGGAAGTGAAAACCGTTCCCGCTGTTCCTTATAAGGATCCAAATAACTATGTAAAGGTTTCGGGTAGAA
TCGCTCAAGAGATGGGTAACGCTGTTTGGGCCAATCAATTCGATAACCTTGCTAATCGAGAAGCGCATTATAAAACCACA
GGTCCGGAAATTTGGAAACAGACGGAAGGACGGTTAGATGTGTGGATCACTTCTCTCGGAACCGGAGGGACTTACGCAGG
AGTTTCGTTATTTTTAAAGGAAAAAGATTCCAAGATCAAAACCGTGGTCGCCGACCCGTACGGATCGGGAATTTATAATT
TTGTAAAAAAGGGTGAGGTTTTTGCGGAAGGTAGTTCCTTTACCGAAGGAATCGGAAACGGAAGGATTACCGAAAACATG
AAAGGGGCGCCTATGGACGATGCGGTTCGAATCACGGATGAGGAATGTCTGAAAGTCGTTTATCAACTTCTTTATAAGGA
TGGGCTTTTTTTAGGAGGTTCGTCCGGAATCAACGTGGCCGCAGCGGTACAACTCGCCAAAGAACTCGGACCGGGTCATA
CTATTGTTACTGTACTATGCGATTCCGGAGCTAGATACCAGTCTCGAATTTTTAATCCGGAATGGTTGATGTCGAAAGGT
TATTCCGTTCCTAAGTTTTGA

Upstream 100 bases:

>100_bases
TAATCCGGTTTTTGTTTGCGAGGACAAGGCTGTCCATTTTTTGAACTCCTACATTCTAAAGCTTGATGGTGTAAAATTTA
CGGAATGAGAAGTAAATGTT

Downstream 100 bases:

>100_bases
GTTTGTGATTCTTTCGAAACAATGGAACTTTATCCGTAAATCTTTTTTTGGGGATTTTCATCTTTCAAAAAACGGGTTCC
TATAGTCGACCTTACAAGTT

Product: cysteine synthase A

Products: NA

Alternate protein names: Beta-thionase; Serine sulfhydrase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNVKKDFSDAVGNTPLILLRSFSEETGCNIYGKAEFLNPGGSVKDRAALFIVEDSERKGLLKAGGTVVEGTAGNTGIGLT
HICNSKGYKTLIIIPDTQSKEKIDLLRTLGAEVKTVPAVPYKDPNNYVKVSGRIAQEMGNAVWANQFDNLANREAHYKTT
GPEIWKQTEGRLDVWITSLGTGGTYAGVSLFLKEKDSKIKTVVADPYGSGIYNFVKKGEVFAEGSSFTEGIGNGRITENM
KGAPMDDAVRITDEECLKVVYQLLYKDGLFLGGSSGINVAAAVQLAKELGPGHTIVTVLCDSGARYQSRIFNPEWLMSKG
YSVPKF

Sequences:

>Translated_326_residues
MNVKKDFSDAVGNTPLILLRSFSEETGCNIYGKAEFLNPGGSVKDRAALFIVEDSERKGLLKAGGTVVEGTAGNTGIGLT
HICNSKGYKTLIIIPDTQSKEKIDLLRTLGAEVKTVPAVPYKDPNNYVKVSGRIAQEMGNAVWANQFDNLANREAHYKTT
GPEIWKQTEGRLDVWITSLGTGGTYAGVSLFLKEKDSKIKTVVADPYGSGIYNFVKKGEVFAEGSSFTEGIGNGRITENM
KGAPMDDAVRITDEECLKVVYQLLYKDGLFLGGSSGINVAAAVQLAKELGPGHTIVTVLCDSGARYQSRIFNPEWLMSKG
YSVPKF
>Mature_326_residues
MNVKKDFSDAVGNTPLILLRSFSEETGCNIYGKAEFLNPGGSVKDRAALFIVEDSERKGLLKAGGTVVEGTAGNTGIGLT
HICNSKGYKTLIIIPDTQSKEKIDLLRTLGAEVKTVPAVPYKDPNNYVKVSGRIAQEMGNAVWANQFDNLANREAHYKTT
GPEIWKQTEGRLDVWITSLGTGGTYAGVSLFLKEKDSKIKTVVADPYGSGIYNFVKKGEVFAEGSSFTEGIGNGRITENM
KGAPMDDAVRITDEECLKVVYQLLYKDGLFLGGSSGINVAAAVQLAKELGPGHTIVTVLCDSGARYQSRIFNPEWLMSKG
YSVPKF

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=320, Percent_Identity=37.1875, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI295821200, Length=320, Percent_Identity=37.1875, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI4557415, Length=320, Percent_Identity=37.1875, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI11345492, Length=292, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI2367138, Length=306, Percent_Identity=34.9673202614379, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1788754, Length=308, Percent_Identity=37.6623376623377, Blast_Score=154, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17562970, Length=304, Percent_Identity=37.5, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI32566674, Length=304, Percent_Identity=37.5, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17535051, Length=313, Percent_Identity=37.0607028753994, Blast_Score=190, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17561720, Length=305, Percent_Identity=37.7049180327869, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI115535073, Length=312, Percent_Identity=33.3333333333333, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17534315, Length=325, Percent_Identity=33.8461538461538, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI25147552, Length=322, Percent_Identity=33.2298136645963, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32566672, Length=214, Percent_Identity=33.1775700934579, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71996324, Length=314, Percent_Identity=25.4777070063694, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321449, Length=331, Percent_Identity=44.1087613293051, Blast_Score=238, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6321594, Length=329, Percent_Identity=36.1702127659575, Blast_Score=189, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24643623, Length=328, Percent_Identity=35.0609756097561, Blast_Score=187, Evalue=8e-48,
Organism=Drosophila melanogaster, GI20129101, Length=328, Percent_Identity=35.0609756097561, Blast_Score=187, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005857
- InterPro:   IPR000644
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00571 CBS; PF00291 PALP [H]

EC number: =4.2.1.22 [H]

Molecular weight: Translated: 35253; Mature: 35253

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVKKDFSDAVGNTPLILLRSFSEETGCNIYGKAEFLNPGGSVKDRAALFIVEDSERKGL
CCCCCCHHHHHCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCH
LKAGGTVVEGTAGNTGIGLTHICNSKGYKTLIIIPDTQSKEKIDLLRTLGAEVKTVPAVP
HHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCEECCCCC
YKDPNNYVKVSGRIAQEMGNAVWANQFDNLANREAHYKTTGPEIWKQTEGRLDVWITSLG
CCCCCCEEEECCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEEECC
TGGTYAGVSLFLKEKDSKIKTVVADPYGSGIYNFVKKGEVFAEGSSFTEGIGNGRITENM
CCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHCCCEEECCCCHHHCCCCCCCCCCC
KGAPMDDAVRITDEECLKVVYQLLYKDGLFLGGSSGINVAAAVQLAKELGPGHTIVTVLC
CCCCCCCEEEECHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEE
DSGARYQSRIFNPEWLMSKGYSVPKF
CCCCHHHHHCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MNVKKDFSDAVGNTPLILLRSFSEETGCNIYGKAEFLNPGGSVKDRAALFIVEDSERKGL
CCCCCCHHHHHCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCH
LKAGGTVVEGTAGNTGIGLTHICNSKGYKTLIIIPDTQSKEKIDLLRTLGAEVKTVPAVP
HHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCEECCCCC
YKDPNNYVKVSGRIAQEMGNAVWANQFDNLANREAHYKTTGPEIWKQTEGRLDVWITSLG
CCCCCCEEEECCHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEEECC
TGGTYAGVSLFLKEKDSKIKTVVADPYGSGIYNFVKKGEVFAEGSSFTEGIGNGRITENM
CCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHCCCEEECCCCHHHCCCCCCCCCCC
KGAPMDDAVRITDEECLKVVYQLLYKDGLFLGGSSGINVAAAVQLAKELGPGHTIVTVLC
CCCCCCCEEEECHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEE
DSGARYQSRIFNPEWLMSKGYSVPKF
CCCCHHHHHCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]