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The map label for this gene is ubiB [C]

Identifier: 116327850

GI number: 116327850

Start: 1329033

End: 1330325

Strand: Reverse

Name: ubiB [C]

Synonym: LBL_1128

Alternate gene names: 116327850

Gene position: 1330325-1329033 (Counterclockwise)

Preceding gene: 116327852

Following gene: 116327849

Centisome position: 36.81

GC content: 43.39

Gene sequence:

>1293_bases
ATGGCCGGTTTTTTTGATTCCCTGAAAATGGGTTTTGGCGGTGCCTCCCGAATCCTGAACAGCAGTTTTGTATTCTCCAC
CAAAACGCTCCTTCTCTTAAAAGACCTTGCATTAGGCGGAGATACCTTCCCAATTCGTCTCCGGGAAGCGTTTGAAGAGT
TAGGCGCCACATACATCAAGTTAGGTCAATTGATCGCTTCTGCCCCCTCTCTTTTTCCCGAAGAATTTGTAACCGAAATG
CAGAAGTGTTTGGATTCTATTCGGCCCATTCCCTTTACCACCGTAGAAAAGATCATTCAAAAGGAATTAGGTGGAAAACT
TACCGATCATTTTCGAAGTGTGGAATCGATCCCGATTGCTTCCGCCTCCATCGCTCAAGTACATTCCGCGATTACTAAAG
ACGGATTAGACGTGGTGATTAAAGTGCAAAGACCGGACATAGAATCCACGTTATCCGCCGATTTAAATCTCATTTATCTT
GCGTCCGTTCTCTTCGAAAGATTTGCGCCGGGACTAAACAAGTCGGGAATTTCCGATATGGTGGGACAATTCCAAACATC
CATATTAGAAGAAATTGATTTTTATAAGGAAGCGGACAACATCGAAGAGTTCGAAAAAGAACTTCTTTTTATGGGAGAAA
CGAGAGCCAGAGTTCCCAAAGTTTACAGGGAATTATCCACAAAGAAAATTCTTACGATGGAACGTTTCTACGGCGCTCCG
ATCACCGACCGGAATTCGGTCCAAAAGTATTCTCCCGATCCACAAAAGATTTTGACGGACGCTCTGGAAATTTGGTTTTC
CACTCTCTCTAGAAACGGTTTCTTCCATGCGGACGTACATGCAGGAAATTTAATGATTCTTCGTGACGGGACCGTGGGGT
TTATCGACTTCGGTATCGTGGGAAGAATTTCTCCTCGAGTCTGGGACGGTCTGATGATTTTCCTGGAGGGTTTAAGCCTC
AATCAAACCAAAAAAATTGCAAAAGGTCTGATCCAGATGGATTCTACCGCCAAAGACGTGAATGAAAAAAAGCTCGCGCA
GGATTTGGAAGTCGTCTTTTCCAGGATGACCGAAATGGTGATGAAAGTCCAAGCGGGAGATTTAGAATCCCTAGACGATA
AAAAATTAAACGCGATTCTTTTCGAGTTCCGTGAAATCTCTCTCAGGAACGGTTTAAAAATTCCGAAAGAATTCGGTCTT
CTTATCAAACAGATTCTTTATTTCGATCGGTACGTAAAAACGATGGCGCCTGAGATCGATCTAATTCGGGACAGGGATAA
ATTCTTAATATGA

Upstream 100 bases:

>100_bases
GTAAACAAAAAAGCAGTTTCGACCCGCAGGCATTCGCCGGTTTTTTCCGGTTCCCTTTCTGTAGTTGTCTTTTGAATCGA
AGTTCCAAATCTTTGTGTTT

Downstream 100 bases:

>100_bases
TTCATTTGTTAAGCGAATTGGAAAAGGGCCAGGAAGCCTCCATCGTCTCCTTACTTTCTCAGCCCGGAAAAGAGGAACTA
TTCCAAAATATCCTGGATAT

Product: protein kinase

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAGFFDSLKMGFGGASRILNSSFVFSTKTLLLLKDLALGGDTFPIRLREAFEELGATYIKLGQLIASAPSLFPEEFVTEM
QKCLDSIRPIPFTTVEKIIQKELGGKLTDHFRSVESIPIASASIAQVHSAITKDGLDVVIKVQRPDIESTLSADLNLIYL
ASVLFERFAPGLNKSGISDMVGQFQTSILEEIDFYKEADNIEEFEKELLFMGETRARVPKVYRELSTKKILTMERFYGAP
ITDRNSVQKYSPDPQKILTDALEIWFSTLSRNGFFHADVHAGNLMILRDGTVGFIDFGIVGRISPRVWDGLMIFLEGLSL
NQTKKIAKGLIQMDSTAKDVNEKKLAQDLEVVFSRMTEMVMKVQAGDLESLDDKKLNAILFEFREISLRNGLKIPKEFGL
LIKQILYFDRYVKTMAPEIDLIRDRDKFLI

Sequences:

>Translated_430_residues
MAGFFDSLKMGFGGASRILNSSFVFSTKTLLLLKDLALGGDTFPIRLREAFEELGATYIKLGQLIASAPSLFPEEFVTEM
QKCLDSIRPIPFTTVEKIIQKELGGKLTDHFRSVESIPIASASIAQVHSAITKDGLDVVIKVQRPDIESTLSADLNLIYL
ASVLFERFAPGLNKSGISDMVGQFQTSILEEIDFYKEADNIEEFEKELLFMGETRARVPKVYRELSTKKILTMERFYGAP
ITDRNSVQKYSPDPQKILTDALEIWFSTLSRNGFFHADVHAGNLMILRDGTVGFIDFGIVGRISPRVWDGLMIFLEGLSL
NQTKKIAKGLIQMDSTAKDVNEKKLAQDLEVVFSRMTEMVMKVQAGDLESLDDKKLNAILFEFREISLRNGLKIPKEFGL
LIKQILYFDRYVKTMAPEIDLIRDRDKFLI
>Mature_429_residues
AGFFDSLKMGFGGASRILNSSFVFSTKTLLLLKDLALGGDTFPIRLREAFEELGATYIKLGQLIASAPSLFPEEFVTEMQ
KCLDSIRPIPFTTVEKIIQKELGGKLTDHFRSVESIPIASASIAQVHSAITKDGLDVVIKVQRPDIESTLSADLNLIYLA
SVLFERFAPGLNKSGISDMVGQFQTSILEEIDFYKEADNIEEFEKELLFMGETRARVPKVYRELSTKKILTMERFYGAPI
TDRNSVQKYSPDPQKILTDALEIWFSTLSRNGFFHADVHAGNLMILRDGTVGFIDFGIVGRISPRVWDGLMIFLEGLSLN
QTKKIAKGLIQMDSTAKDVNEKKLAQDLEVVFSRMTEMVMKVQAGDLESLDDKKLNAILFEFREISLRNGLKIPKEFGLL
IKQILYFDRYVKTMAPEIDLIRDRDKFLI

Specific function: Required, Probably Indirectly, For The Hydroxylation Of 2-Octaprenylphenol To 2-Octaprenyl-6-Hydroxy-Phenol, The Fourth Step In Ubiquinone Biosynthesis. Specific For Aerobically Grown Log-Phase Cells. [C]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. ADCK protein kinase family [H]

Homologues:

Organism=Homo sapiens, GI40254938, Length=313, Percent_Identity=26.8370607028754, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI217035081, Length=210, Percent_Identity=27.6190476190476, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI41393593, Length=250, Percent_Identity=27.2, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI27363457, Length=247, Percent_Identity=25.5060728744939, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI217416386, Length=211, Percent_Identity=26.5402843601896, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI2367309, Length=391, Percent_Identity=26.3427109974425, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17559152, Length=288, Percent_Identity=26.7361111111111, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI32565180, Length=187, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323282, Length=267, Percent_Identity=29.5880149812734, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321319, Length=256, Percent_Identity=25.390625, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325148, Length=260, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI22024280, Length=234, Percent_Identity=32.0512820512821, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24662575, Length=307, Percent_Identity=26.3843648208469, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI18859849, Length=198, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR000719 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 48486; Mature: 48355

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGFFDSLKMGFGGASRILNSSFVFSTKTLLLLKDLALGGDTFPIRLREAFEELGATYIK
CCCCHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LGQLIASAPSLFPEEFVTEMQKCLDSIRPIPFTTVEKIIQKELGGKLTDHFRSVESIPIA
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
SASIAQVHSAITKDGLDVVIKVQRPDIESTLSADLNLIYLASVLFERFAPGLNKSGISDM
HHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHH
VGQFQTSILEEIDFYKEADNIEEFEKELLFMGETRARVPKVYRELSTKKILTMERFYGAP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ITDRNSVQKYSPDPQKILTDALEIWFSTLSRNGFFHADVHAGNLMILRDGTVGFIDFGIV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEECCHH
GRISPRVWDGLMIFLEGLSLNQTKKIAKGLIQMDSTAKDVNEKKLAQDLEVVFSRMTEMV
HCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
MKVQAGDLESLDDKKLNAILFEFREISLRNGLKIPKEFGLLIKQILYFDRYVKTMAPEID
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
LIRDRDKFLI
HHCCCCCCCC
>Mature Secondary Structure 
AGFFDSLKMGFGGASRILNSSFVFSTKTLLLLKDLALGGDTFPIRLREAFEELGATYIK
CCCHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LGQLIASAPSLFPEEFVTEMQKCLDSIRPIPFTTVEKIIQKELGGKLTDHFRSVESIPIA
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
SASIAQVHSAITKDGLDVVIKVQRPDIESTLSADLNLIYLASVLFERFAPGLNKSGISDM
HHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHH
VGQFQTSILEEIDFYKEADNIEEFEKELLFMGETRARVPKVYRELSTKKILTMERFYGAP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ITDRNSVQKYSPDPQKILTDALEIWFSTLSRNGFFHADVHAGNLMILRDGTVGFIDFGIV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEECCHH
GRISPRVWDGLMIFLEGLSLNQTKKIAKGLIQMDSTAKDVNEKKLAQDLEVVFSRMTEMV
HCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
MKVQAGDLESLDDKKLNAILFEFREISLRNGLKIPKEFGLLIKQILYFDRYVKTMAPEID
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
LIRDRDKFLI
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]