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The map label for this gene is groEL

Identifier: 116327829

GI number: 116327829

Start: 1295162

End: 1296802

Strand: Reverse

Name: groEL

Synonym: LBL_1102

Alternate gene names: 116327829

Gene position: 1296802-1295162 (Counterclockwise)

Preceding gene: 116327830

Following gene: 116327828

Centisome position: 35.88

GC content: 47.04

Gene sequence:

>1641_bases
ATGGCTAAAGATATTGAATACAACGAAACAGCAAGACGTAAACTCCTAGAAGGGGTGAACAAACTCGCCAATGCGGTAAA
AGTTACTCTCGGACCCAAAGGTCGTAACGTAGTAATCGATAAAAAATTCGGAGCGCCAACCATCACAAAAGATGGTGTGA
CCGTTGCGAAAGAAATCGAACTTGAAGACGCTCTCGAAAACATGGGAGCACAAATGGTAAAAGAAGTTTCCACAAAAACA
AACGACGTCGCAGGGGACGGAACTACTACTGCAACCATTCTCGCCCAATCTATTATCAACGAGGGTCTGAAAAACGTAAC
GGCTGGCGCTAACCCCATGTCTCTTAAAAGAGGGATCGACAAAGCGGTGACTGCGGCAGTGGAAAGCATTCGAAAAAGAG
CGGTTAAGATCGAAAACAAAAAAGACATCGCAAACGTCGCCAGCATCTCGGCGAACAACGATGATACGATCGGAAATCTA
ATCGCAGACGCTATGGATAAAGTTGGAAAAGACGGAGTGATCACGGTCGAAGAGGCGAAGTCCATCGAGACTACTCTCGA
CGTGGTGGAAGGAATGCAATTCGATAGAGGATATATTTCTCCCTACATGGTCACCGACGCGGAATCTATGGTTGCTACTT
TAAGTGATCCTTATATCCTGATCTACGACAAAAAGATCTCATCCATGAAAGATCTCATCCATATTCTCGAGAAAGTAGCT
CAAGCGGGTAAACCTCTTGTGATCATCTCCGAAGAAGTGGAAGGAGAAGCGCTGGCAACCATCGTCGTCAACACTCTCAG
GAAAACTATCTCCTGTGTTGCGGTTAAAGCTCCGGGCTTCGGAGACAGAAGAAAAGCAATGCTCGAAGATATCGCTATTT
TGACCGGTGGACAAGTCATTTCAGAAGATCTTGGTATGAAACTCGAAAACACTACCCTTCAAATGCTCGGCCGTGCGAAC
AAAGTGACCGTAGATAAAGAGAACACTACGATCATCGAAGGGAAAGGACAAACGAAGGAAATCCAAGGAAGAATCGGTCA
AATCAAAAAACAAATCGAAGATACCACTTCCGAATATGACAAAGAAAAACTCCAAGAAAGACTCGCAAAACTCGCGGGTG
GAGTTGCGGTCATTCATGTCGGAGCGGCTACCGAAGTGGAAATGAAAGAGAAAAAAGCCCGTGTGGAAGACGCTCTTTCC
GCGACCCGCGCGGCAGTGGAAGAAGGGATCGTTCCCGGCGGCGGTTTGACACTTCTCAAAGCTCAAGAAGCTGTTGCCGC
TCTGAAACTGGAAGGCGACGAAGCTACGGGCGCAAAAATTATTTTCCGTTCTTTGGAAGAACCGATTCGTATGATCACAA
ACAACGCAGGTCTGGAAGGATCCGTGATCGTAGAACATGCAAAGACTAAAAAAGGAAACGAAGGTTTTAACGCTCTGTCC
ATGGTTTGGGAAGATCTGGTTTCTGCGGGTGTCGTAGACCCTGCGAAAGTGGTTCGTTCTGCATTACAAAACGCGGCTTC
CATCGGTTCCATGATTTTAACCACCGAAGTCACGATCACCGATAAGCCGGAAAAAGACGCTCCAAACCCGATGGCAGGAA
TGGGCGGAGGCGGTATGGGAGGAATGGGCGGAATGATGTAA

Upstream 100 bases:

>100_bases
AAATATTCCGGAACTGAAATCAAATCGGAAGGCAAAGAATATCTCATCATCCGTGAGAGCGATATTCTGGCCGTCGTTAA
AAAATAATCAGGAGTAAATC

Downstream 100 bases:

>100_bases
TTCATCCACACCCATTTTTACCAAATCGCAAAAGGCGGTTGAGTTTATACTCTTCCGCCTTTTTTGTATAAAATTTTATA
TAGAACGCGAATCCGATCTA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MAKDIEYNETARRKLLEGVNKLANAVKVTLGPKGRNVVIDKKFGAPTITKDGVTVAKEIELEDALENMGAQMVKEVSTKT
NDVAGDGTTTATILAQSIINEGLKNVTAGANPMSLKRGIDKAVTAAVESIRKRAVKIENKKDIANVASISANNDDTIGNL
IADAMDKVGKDGVITVEEAKSIETTLDVVEGMQFDRGYISPYMVTDAESMVATLSDPYILIYDKKISSMKDLIHILEKVA
QAGKPLVIISEEVEGEALATIVVNTLRKTISCVAVKAPGFGDRRKAMLEDIAILTGGQVISEDLGMKLENTTLQMLGRAN
KVTVDKENTTIIEGKGQTKEIQGRIGQIKKQIEDTTSEYDKEKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALS
ATRAAVEEGIVPGGGLTLLKAQEAVAALKLEGDEATGAKIIFRSLEEPIRMITNNAGLEGSVIVEHAKTKKGNEGFNALS
MVWEDLVSAGVVDPAKVVRSALQNAASIGSMILTTEVTITDKPEKDAPNPMAGMGGGGMGGMGGMM

Sequences:

>Translated_546_residues
MAKDIEYNETARRKLLEGVNKLANAVKVTLGPKGRNVVIDKKFGAPTITKDGVTVAKEIELEDALENMGAQMVKEVSTKT
NDVAGDGTTTATILAQSIINEGLKNVTAGANPMSLKRGIDKAVTAAVESIRKRAVKIENKKDIANVASISANNDDTIGNL
IADAMDKVGKDGVITVEEAKSIETTLDVVEGMQFDRGYISPYMVTDAESMVATLSDPYILIYDKKISSMKDLIHILEKVA
QAGKPLVIISEEVEGEALATIVVNTLRKTISCVAVKAPGFGDRRKAMLEDIAILTGGQVISEDLGMKLENTTLQMLGRAN
KVTVDKENTTIIEGKGQTKEIQGRIGQIKKQIEDTTSEYDKEKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALS
ATRAAVEEGIVPGGGLTLLKAQEAVAALKLEGDEATGAKIIFRSLEEPIRMITNNAGLEGSVIVEHAKTKKGNEGFNALS
MVWEDLVSAGVVDPAKVVRSALQNAASIGSMILTTEVTITDKPEKDAPNPMAGMGGGGMGGMGGMM
>Mature_545_residues
AKDIEYNETARRKLLEGVNKLANAVKVTLGPKGRNVVIDKKFGAPTITKDGVTVAKEIELEDALENMGAQMVKEVSTKTN
DVAGDGTTTATILAQSIINEGLKNVTAGANPMSLKRGIDKAVTAAVESIRKRAVKIENKKDIANVASISANNDDTIGNLI
ADAMDKVGKDGVITVEEAKSIETTLDVVEGMQFDRGYISPYMVTDAESMVATLSDPYILIYDKKISSMKDLIHILEKVAQ
AGKPLVIISEEVEGEALATIVVNTLRKTISCVAVKAPGFGDRRKAMLEDIAILTGGQVISEDLGMKLENTTLQMLGRANK
VTVDKENTTIIEGKGQTKEIQGRIGQIKKQIEDTTSEYDKEKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALSA
TRAAVEEGIVPGGGLTLLKAQEAVAALKLEGDEATGAKIIFRSLEEPIRMITNNAGLEGSVIVEHAKTKKGNEGFNALSM
VWEDLVSAGVVDPAKVVRSALQNAASIGSMILTTEVTITDKPEKDAPNPMAGMGGGGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=529, Percent_Identity=48.5822306238185, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI31542947, Length=529, Percent_Identity=48.5822306238185, Blast_Score=489, Evalue=1e-138,
Organism=Escherichia coli, GI1790586, Length=529, Percent_Identity=61.6257088846881, Blast_Score=653, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=530, Percent_Identity=46.7924528301887, Blast_Score=483, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI193210679, Length=211, Percent_Identity=46.9194312796209, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI25147750, Length=559, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=50.381679389313, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6322524, Length=575, Percent_Identity=21.7391304347826, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6322350, Length=559, Percent_Identity=22.8980322003578, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641193, Length=532, Percent_Identity=48.1203007518797, Blast_Score=502, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24641191, Length=532, Percent_Identity=48.1203007518797, Blast_Score=502, Evalue=1e-142,
Organism=Drosophila melanogaster, GI45550936, Length=524, Percent_Identity=45.2290076335878, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI45550132, Length=524, Percent_Identity=45.2290076335878, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI45550935, Length=524, Percent_Identity=45.2290076335878, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864606, Length=553, Percent_Identity=39.7830018083183, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24584129, Length=529, Percent_Identity=34.2155009451796, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI19921262, Length=529, Percent_Identity=34.2155009451796, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI18858175, Length=567, Percent_Identity=24.3386243386243, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571140, Length=567, Percent_Identity=24.3386243386243, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_LEPBJ (Q04S01)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801077.1
- ProteinModelPortal:   Q04S01
- SMR:   Q04S01
- STRING:   Q04S01
- GeneID:   4410503
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1772
- eggNOG:   COG0459
- HOGENOM:   HBG625289
- OMA:   EDYVDMV
- PhylomeDB:   Q04S01
- ProtClustDB:   PRK00013
- BioCyc:   LBOR355277:LBJ_1772-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57931; Mature: 57799

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDIEYNETARRKLLEGVNKLANAVKVTLGPKGRNVVIDKKFGAPTITKDGVTVAKEIE
CCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHH
LEDALENMGAQMVKEVSTKTNDVAGDGTTTATILAQSIINEGLKNVTAGANPMSLKRGID
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KAVTAAVESIRKRAVKIENKKDIANVASISANNDDTIGNLIADAMDKVGKDGVITVEEAK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHH
SIETTLDVVEGMQFDRGYISPYMVTDAESMVATLSDPYILIYDKKISSMKDLIHILEKVA
HHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHH
QAGKPLVIISEEVEGEALATIVVNTLRKTISCVAVKAPGFGDRRKAMLEDIAILTGGQVI
HCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCHHH
SEDLGMKLENTTLQMLGRANKVTVDKENTTIIEGKGQTKEIQGRIGQIKKQIEDTTSEYD
HHHHCCEECHHHHHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
KEKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALSATRAAVEEGIVPGGGLTLLK
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
AQEAVAALKLEGDEATGAKIIFRSLEEPIRMITNNAGLEGSVIVEHAKTKKGNEGFNALS
HHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCCCHHHHH
MVWEDLVSAGVVDPAKVVRSALQNAASIGSMILTTEVTITDKPEKDAPNPMAGMGGGGMG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
GMGGMM
CCCCCC
>Mature Secondary Structure 
AKDIEYNETARRKLLEGVNKLANAVKVTLGPKGRNVVIDKKFGAPTITKDGVTVAKEIE
CCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHH
LEDALENMGAQMVKEVSTKTNDVAGDGTTTATILAQSIINEGLKNVTAGANPMSLKRGID
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KAVTAAVESIRKRAVKIENKKDIANVASISANNDDTIGNLIADAMDKVGKDGVITVEEAK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHH
SIETTLDVVEGMQFDRGYISPYMVTDAESMVATLSDPYILIYDKKISSMKDLIHILEKVA
HHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHH
QAGKPLVIISEEVEGEALATIVVNTLRKTISCVAVKAPGFGDRRKAMLEDIAILTGGQVI
HCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCHHH
SEDLGMKLENTTLQMLGRANKVTVDKENTTIIEGKGQTKEIQGRIGQIKKQIEDTTSEYD
HHHHCCEECHHHHHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
KEKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALSATRAAVEEGIVPGGGLTLLK
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
AQEAVAALKLEGDEATGAKIIFRSLEEPIRMITNNAGLEGSVIVEHAKTKKGNEGFNALS
HHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCCCHHHHH
MVWEDLVSAGVVDPAKVVRSALQNAASIGSMILTTEVTITDKPEKDAPNPMAGMGGGGMG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
GMGGMM
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA