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The map label for this gene is thiH [H]

Identifier: 116327779

GI number: 116327779

Start: 1213526

End: 1214626

Strand: Direct

Name: thiH [H]

Synonym: LBL_1039

Alternate gene names: 116327779

Gene position: 1213526-1214626 (Clockwise)

Preceding gene: 116327778

Following gene: 116327780

Centisome position: 33.57

GC content: 45.41

Gene sequence:

>1101_bases
GTGGCCTCTATCTTTTCATCTCAACCCAGCGATCGGATCTTGGAAAAAGCTTTGGATGGGAATCGGATTTCTCCCGAGGA
AGCTCTGACCCTTTATCGGGAAGGGGATCATCTCAAGATCATGGCTACGGCTCGTACACTTCGAGAAAAAATTCTCCCTC
ATACACATGCAAGTTATACTATGTTTCGAGTCGTCAATTACACAAACTACTGCAACGTAGAATGTAGTTTCTGTTCTTTT
ATGGACGAAATCGGAAACGGAAAAGGATACGTACTTTCCGTGGAAGAAATATTGGAAAAGATGGATTATGCCGTCGGCGA
AGGGGCGGATCAAATGTTTCTACAAGGAGGGGTATATCCGGATCTTCCTTTTGAGTATTATATAAATGTGATAAGCTCCG
TTAAAAAGAAATATCCGGACATGCACATCCGCGCATTTTCTCCGGTGGAAATCATCAACTTGGAAAAGATCACCGGCCTT
TCACTTAAGGAAGTGCTTCGGATTCTAAAACAAGCGGGTCTTGATTCTGTTCCCGGAGCGGGAGCGGAGATTCTCACCGA
TAGAATGAGAAACATCATCTCGCCGAAAAAAGCGACCACGGAAGAATGGGTGCGTGCGATGGAAACCTGCCATGAAGCGG
GACTTCCCGGAAGCGCAAACATCGTATTTGGTTCCGAGGAAACCCGGGAAGAAGTCGTCGAACATCTAAGCGTTGTGCGA
AATCTCCAGGACAGAACCGGAGGATTTTTATCTTTTATCCCTTGGACTTTTCAACCACAAACGAAACGATTCAAGGTCCG
CGCGGTTGCAACTCAGGAGTATCTCAAGGTGCTGGGAATTTGCAGGATCTTTTTGGATAATATTTCACATATCGAAACTT
CGGTGATGGTACTCGGGAAAGGAGTCGGACAATTGGCTCTTACGAGCGGTGCGGACGATATATCTTCCGTGGTTATCGAA
GAAAACGTACTTCGGTCTTACGGACTCAAAACCGAAAAAGAAGCGGTTAAGTTTTTGAAAGAAGGCGGATTTACACCGAA
ACGAAGAGATCTTTTCTATAACTACGATCGTTACGGGAATGAATTGACGCAGGCCCTTTGA

Upstream 100 bases:

>100_bases
AAGAACCTAGTTTGCGGAAACTTCGGTATTTTATTACGAACTTGTTGAATGATTGGAATGGATTTCTTTCAGATTTTGGA
AAGGCTGATAAGGAGTAAAC

Downstream 100 bases:

>100_bases
TCGTTTGAACTTTTCCGAATGGAAAGCGAGATCCGTTGTGATACAAGGAGGGTCTTAAAAATCGCTTTTGAAAAATTGAA
GAGTGTCTTTAGGGAGATAA

Product: thiamine biosynthesis enzyme

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MASIFSSQPSDRILEKALDGNRISPEEALTLYREGDHLKIMATARTLREKILPHTHASYTMFRVVNYTNYCNVECSFCSF
MDEIGNGKGYVLSVEEILEKMDYAVGEGADQMFLQGGVYPDLPFEYYINVISSVKKKYPDMHIRAFSPVEIINLEKITGL
SLKEVLRILKQAGLDSVPGAGAEILTDRMRNIISPKKATTEEWVRAMETCHEAGLPGSANIVFGSEETREEVVEHLSVVR
NLQDRTGGFLSFIPWTFQPQTKRFKVRAVATQEYLKVLGICRIFLDNISHIETSVMVLGKGVGQLALTSGADDISSVVIE
ENVLRSYGLKTEKEAVKFLKEGGFTPKRRDLFYNYDRYGNELTQAL

Sequences:

>Translated_366_residues
MASIFSSQPSDRILEKALDGNRISPEEALTLYREGDHLKIMATARTLREKILPHTHASYTMFRVVNYTNYCNVECSFCSF
MDEIGNGKGYVLSVEEILEKMDYAVGEGADQMFLQGGVYPDLPFEYYINVISSVKKKYPDMHIRAFSPVEIINLEKITGL
SLKEVLRILKQAGLDSVPGAGAEILTDRMRNIISPKKATTEEWVRAMETCHEAGLPGSANIVFGSEETREEVVEHLSVVR
NLQDRTGGFLSFIPWTFQPQTKRFKVRAVATQEYLKVLGICRIFLDNISHIETSVMVLGKGVGQLALTSGADDISSVVIE
ENVLRSYGLKTEKEAVKFLKEGGFTPKRRDLFYNYDRYGNELTQAL
>Mature_365_residues
ASIFSSQPSDRILEKALDGNRISPEEALTLYREGDHLKIMATARTLREKILPHTHASYTMFRVVNYTNYCNVECSFCSFM
DEIGNGKGYVLSVEEILEKMDYAVGEGADQMFLQGGVYPDLPFEYYINVISSVKKKYPDMHIRAFSPVEIINLEKITGLS
LKEVLRILKQAGLDSVPGAGAEILTDRMRNIISPKKATTEEWVRAMETCHEAGLPGSANIVFGSEETREEVVEHLSVVRN
LQDRTGGFLSFIPWTFQPQTKRFKVRAVATQEYLKVLGICRIFLDNISHIETSVMVLGKGVGQLALTSGADDISSVVIEE
NVLRSYGLKTEKEAVKFLKEGGFTPKRRDLFYNYDRYGNELTQAL

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41181; Mature: 41050

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIFSSQPSDRILEKALDGNRISPEEALTLYREGDHLKIMATARTLREKILPHTHASYT
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHEECCCCEEEEHHHHHHHHHHCCCCCCHHH
MFRVVNYTNYCNVECSFCSFMDEIGNGKGYVLSVEEILEKMDYAVGEGADQMFLQGGVYP
HHHEECCCCCCCCEEHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHCCCCCC
DLPFEYYINVISSVKKKYPDMHIRAFSPVEIINLEKITGLSLKEVLRILKQAGLDSVPGA
CCCHHHHHHHHHHHHHHCCCCEEEECCCEEEEEHHHHCCCCHHHHHHHHHHCCCCCCCCC
GAEILTDRMRNIISPKKATTEEWVRAMETCHEAGLPGSANIVFGSEETREEVVEHLSVVR
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHH
NLQDRTGGFLSFIPWTFQPQTKRFKVRAVATQEYLKVLGICRIFLDNISHIETSVMVLGK
HHHHHCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVGQLALTSGADDISSVVIEENVLRSYGLKTEKEAVKFLKEGGFTPKRRDLFYNYDRYGN
CCCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHEECHHHHHH
ELTQAL
HHHHCC
>Mature Secondary Structure 
ASIFSSQPSDRILEKALDGNRISPEEALTLYREGDHLKIMATARTLREKILPHTHASYT
CCCCCCCCHHHHHHHHCCCCCCCHHHHHHHEECCCCEEEEHHHHHHHHHHCCCCCCHHH
MFRVVNYTNYCNVECSFCSFMDEIGNGKGYVLSVEEILEKMDYAVGEGADQMFLQGGVYP
HHHEECCCCCCCCEEHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHCCCCCC
DLPFEYYINVISSVKKKYPDMHIRAFSPVEIINLEKITGLSLKEVLRILKQAGLDSVPGA
CCCHHHHHHHHHHHHHHCCCCEEEECCCEEEEEHHHHCCCCHHHHHHHHHHCCCCCCCCC
GAEILTDRMRNIISPKKATTEEWVRAMETCHEAGLPGSANIVFGSEETREEVVEHLSVVR
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHH
NLQDRTGGFLSFIPWTFQPQTKRFKVRAVATQEYLKVLGICRIFLDNISHIETSVMVLGK
HHHHHCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVGQLALTSGADDISSVVIEENVLRSYGLKTEKEAVKFLKEGGFTPKRRDLFYNYDRYGN
CCCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHEECHHHHHH
ELTQAL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]