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The map label for this gene is lpdA-2 [H]

Identifier: 116327756

GI number: 116327756

Start: 1181119

End: 1182537

Strand: Reverse

Name: lpdA-2 [H]

Synonym: LBL_1013

Alternate gene names: 116327756

Gene position: 1182537-1181119 (Counterclockwise)

Preceding gene: 116327761

Following gene: 116327754

Centisome position: 32.72

GC content: 47.29

Gene sequence:

>1419_bases
ATGTCAGAATCTTACGATCTCACTGTTATCGGAGCGGGCCCAGGGGGCTATGTCGCCGCGATCCGAGGCGCCCAACTAGG
AATGAACGTATGTATCATTGAAAAAGAAAGGCCGGGTGGAATTTGTTTAAACTGGGGTTGTATTCCGACGAAGGCATTGC
TTGAATCGGCTCATCTTTTAGATAAGATACATTCCGCAAAAGGTTTCGGAATCGATCTCTCTGGCGCGAAACCCGATTTT
CCGTCCATCATTGCGCGTTCCAGAAACGTCGCAGACAGTATGGCTTCGGGCGTGGAATTTCTTCTGAACAAAAATAAGAT
CACGCGTAAAAAAGGAACCGCAGTATTCAAAGATTCGAATACGATTTGGCTTCCCGACACTTCCAAAGAAGAAATCACTT
CGAAATATTATATTCTCGCAACCGGAGCAAGAGCCAGAGAACTTCCCGGTTTACCGTTCGACGCAGGCACGGTCCTTTCC
TCAAAAACTGCGATGATTCAGGAAAAAATTCCGGAGTCCCTTTTGATCGTAGGCGCGGGCGCGATCGGGGTCGAGTTCGC
GGACTTTTATTCCACGATGGGAACAAAAGTCACGTTAGTCGAAATGATGGATCAAATTCTTCCCGTAGAAGATAAAGAGA
TCTCCGCATTTTTAGAAAAGTCTTTTGTTAAAAGAGGAATCCGAGTTCTCACCGGAGTCGGAGTTTCCGACCCCGTGATT
AAAAACGGAAAAGTAAAAGTTCTTCTCAAAGGAAAAAATCTTCCGGAAAGCGGCGAAATATCGGAGACAGAAAAAATTTT
GGTTTCCATCGGACTCGTTCCGAATACGGACACGATGAACCTGGAAGAGATCGGCGTCTTTTTACAAAAAGGTTTCGTAA
AAACGGACTCACAGTACAAAACAAGCATTCCTCATATTTATGCGATCGGAGATTGTAACGGCCCTCCCCTTCTCGCACAC
GTCGCTTCCATGGAAGGCATTAAGGCCGCCGAAGCGATCTCCATCCAGGTTGGAAACCCTCACCACCTTAGTTATACTCC
GATCAACTACGACGCAATTCCGGGTTGTACCTACTGTTATCCCGAAGTCGCCTCCATCGGATTCACCGAAAAAAAAGCGA
CTGACATGGGTTATACGATCAGCGTCGGAAAATTTCCTTTTATCGCCAGTGGTAGAGCCAAAGCGATGGGAGACACGGGT
GGTTTTACGAAAGTGGTCGTGGATAAATCTTCCGGAGAAATTTTAGGAGCCCATCTCATTGGTCCCGGAGTTACGGAACT
TTTACCTGCGGTCGCTCTCGGCATCACTCAGGAACTGACCGCGAAAGAAATCGCATCCACTATCTTTGCACACCCCACGC
TTTCAGAAACCGTGATGGAATCCTTCGGCGCGGCTTTAGGAGAAGCGATCAATCTCTAA

Upstream 100 bases:

>100_bases
GGGTTTGGGACGGACTGAAAATTTTTAGGAACGTTGATTTGATTTCAAAAAATTCTTGCCCAACCTTGATTAAAACCTAT
CCATAACTCCGGAGAATTTT

Downstream 100 bases:

>100_bases
AAAAATCAGTGAATCAGATTCCCAGTCAGCAAGATATCCGTTTTGTCGACCTGAAACTCCGCGGGAAGTTGCGAACTAAG
TGAAAGCAAAGAAGGATCTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSESYDLTVIGAGPGGYVAAIRGAQLGMNVCIIEKERPGGICLNWGCIPTKALLESAHLLDKIHSAKGFGIDLSGAKPDF
PSIIARSRNVADSMASGVEFLLNKNKITRKKGTAVFKDSNTIWLPDTSKEEITSKYYILATGARARELPGLPFDAGTVLS
SKTAMIQEKIPESLLIVGAGAIGVEFADFYSTMGTKVTLVEMMDQILPVEDKEISAFLEKSFVKRGIRVLTGVGVSDPVI
KNGKVKVLLKGKNLPESGEISETEKILVSIGLVPNTDTMNLEEIGVFLQKGFVKTDSQYKTSIPHIYAIGDCNGPPLLAH
VASMEGIKAAEAISIQVGNPHHLSYTPINYDAIPGCTYCYPEVASIGFTEKKATDMGYTISVGKFPFIASGRAKAMGDTG
GFTKVVVDKSSGEILGAHLIGPGVTELLPAVALGITQELTAKEIASTIFAHPTLSETVMESFGAALGEAINL

Sequences:

>Translated_472_residues
MSESYDLTVIGAGPGGYVAAIRGAQLGMNVCIIEKERPGGICLNWGCIPTKALLESAHLLDKIHSAKGFGIDLSGAKPDF
PSIIARSRNVADSMASGVEFLLNKNKITRKKGTAVFKDSNTIWLPDTSKEEITSKYYILATGARARELPGLPFDAGTVLS
SKTAMIQEKIPESLLIVGAGAIGVEFADFYSTMGTKVTLVEMMDQILPVEDKEISAFLEKSFVKRGIRVLTGVGVSDPVI
KNGKVKVLLKGKNLPESGEISETEKILVSIGLVPNTDTMNLEEIGVFLQKGFVKTDSQYKTSIPHIYAIGDCNGPPLLAH
VASMEGIKAAEAISIQVGNPHHLSYTPINYDAIPGCTYCYPEVASIGFTEKKATDMGYTISVGKFPFIASGRAKAMGDTG
GFTKVVVDKSSGEILGAHLIGPGVTELLPAVALGITQELTAKEIASTIFAHPTLSETVMESFGAALGEAINL
>Mature_471_residues
SESYDLTVIGAGPGGYVAAIRGAQLGMNVCIIEKERPGGICLNWGCIPTKALLESAHLLDKIHSAKGFGIDLSGAKPDFP
SIIARSRNVADSMASGVEFLLNKNKITRKKGTAVFKDSNTIWLPDTSKEEITSKYYILATGARARELPGLPFDAGTVLSS
KTAMIQEKIPESLLIVGAGAIGVEFADFYSTMGTKVTLVEMMDQILPVEDKEISAFLEKSFVKRGIRVLTGVGVSDPVIK
NGKVKVLLKGKNLPESGEISETEKILVSIGLVPNTDTMNLEEIGVFLQKGFVKTDSQYKTSIPHIYAIGDCNGPPLLAHV
ASMEGIKAAEAISIQVGNPHHLSYTPINYDAIPGCTYCYPEVASIGFTEKKATDMGYTISVGKFPFIASGRAKAMGDTGG
FTKVVVDKSSGEILGAHLIGPGVTELLPAVALGITQELTAKEIASTIFAHPTLSETVMESFGAALGEAINL

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=477, Percent_Identity=36.0587002096436, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI50301238, Length=474, Percent_Identity=27.2151898734177, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI22035672, Length=485, Percent_Identity=27.6288659793814, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI33519430, Length=473, Percent_Identity=24.5243128964059, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI33519428, Length=473, Percent_Identity=24.5243128964059, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI33519426, Length=473, Percent_Identity=24.5243128964059, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI148277065, Length=473, Percent_Identity=24.5243128964059, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI148277071, Length=473, Percent_Identity=24.5243128964059, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI291045266, Length=439, Percent_Identity=23.9179954441913, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI291045268, Length=480, Percent_Identity=23.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786307, Length=455, Percent_Identity=35.3846153846154, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=29.4243070362473, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1789915, Length=462, Percent_Identity=29.004329004329, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI87081717, Length=473, Percent_Identity=26.8498942917548, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=477, Percent_Identity=34.5911949685535, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17557007, Length=488, Percent_Identity=26.2295081967213, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=455, Percent_Identity=25.7142857142857, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71983419, Length=455, Percent_Identity=25.7142857142857, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=27.8118609406953, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=485, Percent_Identity=36.701030927835, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=27.7083333333333, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=25.7383966244726, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21358499, Length=475, Percent_Identity=36.8421052631579, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=26.875, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24640549, Length=479, Percent_Identity=27.9749478079332, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24640553, Length=479, Percent_Identity=27.9749478079332, Blast_Score=142, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24640551, Length=479, Percent_Identity=27.9749478079332, Blast_Score=141, Evalue=9e-34,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50060; Mature: 49929

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESYDLTVIGAGPGGYVAAIRGAQLGMNVCIIEKERPGGICLNWGCIPTKALLESAHLL
CCCCEEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCCEEEECCCCCHHHHHHHHHHH
DKIHSAKGFGIDLSGAKPDFPSIIARSRNVADSMASGVEFLLNKNKITRKKGTAVFKDSN
HHHHCCCCCCEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEECCCCCEEEECCC
TIWLPDTSKEEITSKYYILATGARARELPGLPFDAGTVLSSKTAMIQEKIPESLLIVGAG
EEECCCCCHHHHCCEEEEEEECCCHHHCCCCCCCCCCHHCCHHHHHHHHCCCEEEEEECC
AIGVEFADFYSTMGTKVTLVEMMDQILPVEDKEISAFLEKSFVKRGIRVLTGVGVSDPVI
HHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCC
KNGKVKVLLKGKNLPESGEISETEKILVSIGLVPNTDTMNLEEIGVFLQKGFVKTDSQYK
CCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCEECCCCCHH
TSIPHIYAIGDCNGPPLLAHVASMEGIKAAEAISIQVGNPHHLSYTPINYDAIPGCTYCY
CCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCEEEECCCCCCCCCCCCCCC
PEVASIGFTEKKATDMGYTISVGKFPFIASGRAKAMGDTGGFTKVVVDKSSGEILGAHLI
HHHHHCCCCCHHCCCCCEEEEECCCCEEECCCCCCCCCCCCEEEEEEECCCCCEEEEEEC
GPGVTELLPAVALGITQELTAKEIASTIFAHPTLSETVMESFGAALGEAINL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SESYDLTVIGAGPGGYVAAIRGAQLGMNVCIIEKERPGGICLNWGCIPTKALLESAHLL
CCCEEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCCEEEECCCCCHHHHHHHHHHH
DKIHSAKGFGIDLSGAKPDFPSIIARSRNVADSMASGVEFLLNKNKITRKKGTAVFKDSN
HHHHCCCCCCEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEECCCCCEEEECCC
TIWLPDTSKEEITSKYYILATGARARELPGLPFDAGTVLSSKTAMIQEKIPESLLIVGAG
EEECCCCCHHHHCCEEEEEEECCCHHHCCCCCCCCCCHHCCHHHHHHHHCCCEEEEEECC
AIGVEFADFYSTMGTKVTLVEMMDQILPVEDKEISAFLEKSFVKRGIRVLTGVGVSDPVI
HHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCC
KNGKVKVLLKGKNLPESGEISETEKILVSIGLVPNTDTMNLEEIGVFLQKGFVKTDSQYK
CCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCEECCCCCHH
TSIPHIYAIGDCNGPPLLAHVASMEGIKAAEAISIQVGNPHHLSYTPINYDAIPGCTYCY
CCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCEEEECCCCCCCCCCCCCCC
PEVASIGFTEKKATDMGYTISVGKFPFIASGRAKAMGDTGGFTKVVVDKSSGEILGAHLI
HHHHHCCCCCHHCCCCCEEEEECCCCEEECCCCCCCCCCCCEEEEEEECCCCCEEEEEEC
GPGVTELLPAVALGITQELTAKEIASTIFAHPTLSETVMESFGAALGEAINL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]