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The map label for this gene is leuA-1 [H]

Identifier: 116327557

GI number: 116327557

Start: 896774

End: 898081

Strand: Reverse

Name: leuA-1 [H]

Synonym: LBL_0786

Alternate gene names: 116327557

Gene position: 898081-896774 (Counterclockwise)

Preceding gene: 116327560

Following gene: 116327555

Centisome position: 24.85

GC content: 43.12

Gene sequence:

>1308_bases
ATGCAACAAAATTCGAAACAAGAAAACGAGTACGTTATTCAGGACATTTCAATATCCGGAAATCAAAAACATATACAGTC
CTTTTCGGAAATCCTTCAAAACCCTCTTCCCAAACATCCGCCTTTTTTTATGGACGTAACTTTGAGAGACGGCAATCAAG
CGCTTCGTAAACCTTGGGATTTAGATCAAAAAGAAACGATCTTCAAACAACTCATCAAACTAGGCGTTCAAGGAATCGAA
GTCGGATTCGCGTCTTCTAATTCTCAAGAATTCAAAACATGCAGTCGCCTTTCTTCTATCGCACCGGATAATGTTGTAAT
ATCCTCTCTTTCCAGAGCGGTCGAAAAGGAAATCAAAATTTCTTGGAAAGCGATTCGACACGCATCGAAACCGAGGATTC
ATATCGTATATCCGATATCCGCATTTACGATTCAAAATGTGTTGAAGTTAAGTCCTGAAAAAGTTTTGGAAAAAATTTCC
GAATCCGTCTCTTTCGCAAAAAGCCTCGTCGGTTCTAAAGGTGAAATTCAATTTTCAGGAGAACATTTCGGAGATTCTCT
GGAGAATTTGGATTTTGCCGTGGAAGCGTTTCGAACGGCGTTGAATTACGGAGCCGATATCATCAATCTTCCGAATACGG
TAGAACGTTATCGTCCTTGGCTTTTTGTTTCTATGGTAAAAGCCGTTAGGAATGTTCTTCCTGAAAACGCAAAAATTTCC
GTGCACACGCATAACGACCTGGGAATGGCAACCGCAACCACAGTTGAATCCTACTTTTCAGGCGCCGTTCAACTAGAGAC
CGCTTTAAACGGCCTTGGGGAAAGGGCCGGAAATACGAACACATACGAGGTTGCAATCGCCCTTCATAACTGCGGCGTCG
ACGTTCCCCTGAACCTTTCGGCAATCTACGAAACATCTCGATTGGTCTCTTACCTTTCGAACGTTCCCATCTATGAAAAA
GCCCCTTTGGTCGGCGAAGACGTGATCTCTCATAGATCCGGAATTTACCAAGATGGTGTCGCAAAAACTAGGCATCTTTC
CAAAGGCGCCTATCGCGCGTTTAACGCAACTTTGATCGGAAGACCCGAAGAAGACCGAATCGAGTTTACAAGCCAATCTG
GAAGAAGTGCGATTTTCTGTATTTTAAAAGACGCAGGAGAGGATATCACCCTGGAACAAGCAGGAAAACTGCAACCGATC
TTAAAGAAGATTTCGGAAGATTCGGGAAAAGGAAAACTGACTCTGAGTGAAATTCAGCTCGTATGGAATACGATGAAGAC
AATTCCTCACAAAGCGGCTTCGAGATAA

Upstream 100 bases:

>100_bases
CACGAGCCCGCTCTCCTTGAGTGGGCTTTTTTGTTTCCGGAAAATTCTTCTCACTCGGAAAAAAACCACTCCTGGAATCG
GCCTCTAAAAGGAGAAAACA

Downstream 100 bases:

>100_bases
GTTTTCTAAAAAAAACTATTTAGGAGCTATTGGATAAGCGGCTTTCGGAGAAAAATTATTTTGTACTTTAATGTTTTATT
GTATTCTTAAAAATAGTTTT

Product: isopropylmalate/homocitrate/citramalatesynthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQQNSKQENEYVIQDISISGNQKHIQSFSEILQNPLPKHPPFFMDVTLRDGNQALRKPWDLDQKETIFKQLIKLGVQGIE
VGFASSNSQEFKTCSRLSSIAPDNVVISSLSRAVEKEIKISWKAIRHASKPRIHIVYPISAFTIQNVLKLSPEKVLEKIS
ESVSFAKSLVGSKGEIQFSGEHFGDSLENLDFAVEAFRTALNYGADIINLPNTVERYRPWLFVSMVKAVRNVLPENAKIS
VHTHNDLGMATATTVESYFSGAVQLETALNGLGERAGNTNTYEVAIALHNCGVDVPLNLSAIYETSRLVSYLSNVPIYEK
APLVGEDVISHRSGIYQDGVAKTRHLSKGAYRAFNATLIGRPEEDRIEFTSQSGRSAIFCILKDAGEDITLEQAGKLQPI
LKKISEDSGKGKLTLSEIQLVWNTMKTIPHKAASR

Sequences:

>Translated_435_residues
MQQNSKQENEYVIQDISISGNQKHIQSFSEILQNPLPKHPPFFMDVTLRDGNQALRKPWDLDQKETIFKQLIKLGVQGIE
VGFASSNSQEFKTCSRLSSIAPDNVVISSLSRAVEKEIKISWKAIRHASKPRIHIVYPISAFTIQNVLKLSPEKVLEKIS
ESVSFAKSLVGSKGEIQFSGEHFGDSLENLDFAVEAFRTALNYGADIINLPNTVERYRPWLFVSMVKAVRNVLPENAKIS
VHTHNDLGMATATTVESYFSGAVQLETALNGLGERAGNTNTYEVAIALHNCGVDVPLNLSAIYETSRLVSYLSNVPIYEK
APLVGEDVISHRSGIYQDGVAKTRHLSKGAYRAFNATLIGRPEEDRIEFTSQSGRSAIFCILKDAGEDITLEQAGKLQPI
LKKISEDSGKGKLTLSEIQLVWNTMKTIPHKAASR
>Mature_435_residues
MQQNSKQENEYVIQDISISGNQKHIQSFSEILQNPLPKHPPFFMDVTLRDGNQALRKPWDLDQKETIFKQLIKLGVQGIE
VGFASSNSQEFKTCSRLSSIAPDNVVISSLSRAVEKEIKISWKAIRHASKPRIHIVYPISAFTIQNVLKLSPEKVLEKIS
ESVSFAKSLVGSKGEIQFSGEHFGDSLENLDFAVEAFRTALNYGADIINLPNTVERYRPWLFVSMVKAVRNVLPENAKIS
VHTHNDLGMATATTVESYFSGAVQLETALNGLGERAGNTNTYEVAIALHNCGVDVPLNLSAIYETSRLVSYLSNVPIYEK
APLVGEDVISHRSGIYQDGVAKTRHLSKGAYRAFNATLIGRPEEDRIEFTSQSGRSAIFCILKDAGEDITLEQAGKLQPI
LKKISEDSGKGKLTLSEIQLVWNTMKTIPHKAASR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=344, Percent_Identity=34.0116279069767, Blast_Score=174, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6324682, Length=397, Percent_Identity=30.2267002518892, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324225, Length=410, Percent_Identity=28.780487804878, Blast_Score=149, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6320019, Length=303, Percent_Identity=24.0924092409241, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320071, Length=306, Percent_Identity=24.5098039215686, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 48214; Mature: 48214

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQNSKQENEYVIQDISISGNQKHIQSFSEILQNPLPKHPPFFMDVTLRDGNQALRKPWD
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHCCCCC
LDQKETIFKQLIKLGVQGIEVGFASSNSQEFKTCSRLSSIAPDNVVISSLSRAVEKEIKI
CCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SWKAIRHASKPRIHIVYPISAFTIQNVLKLSPEKVLEKISESVSFAKSLVGSKGEIQFSG
HHHHHHCCCCCEEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECC
EHFGDSLENLDFAVEAFRTALNYGADIINLPNTVERYRPWLFVSMVKAVRNVLPENAKIS
HHHCCHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
VHTHNDLGMATATTVESYFSGAVQLETALNGLGERAGNTNTYEVAIALHNCGVDVPLNLS
EEECCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHH
AIYETSRLVSYLSNVPIYEKAPLVGEDVISHRSGIYQDGVAKTRHLSKGAYRAFNATLIG
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEEC
RPEEDRIEFTSQSGRSAIFCILKDAGEDITLEQAGKLQPILKKISEDSGKGKLTLSEIQL
CCCHHHEEEECCCCCEEEEEEEECCCCCEEHHHCCCHHHHHHHHCCCCCCCCEEHHHHHH
VWNTMKTIPHKAASR
HHHHHHHCCHHHCCC
>Mature Secondary Structure
MQQNSKQENEYVIQDISISGNQKHIQSFSEILQNPLPKHPPFFMDVTLRDGNQALRKPWD
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHCCCCC
LDQKETIFKQLIKLGVQGIEVGFASSNSQEFKTCSRLSSIAPDNVVISSLSRAVEKEIKI
CCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SWKAIRHASKPRIHIVYPISAFTIQNVLKLSPEKVLEKISESVSFAKSLVGSKGEIQFSG
HHHHHHCCCCCEEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECC
EHFGDSLENLDFAVEAFRTALNYGADIINLPNTVERYRPWLFVSMVKAVRNVLPENAKIS
HHHCCHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
VHTHNDLGMATATTVESYFSGAVQLETALNGLGERAGNTNTYEVAIALHNCGVDVPLNLS
EEECCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHH
AIYETSRLVSYLSNVPIYEKAPLVGEDVISHRSGIYQDGVAKTRHLSKGAYRAFNATLIG
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEEEEC
RPEEDRIEFTSQSGRSAIFCILKDAGEDITLEQAGKLQPILKKISEDSGKGKLTLSEIQL
CCCHHHEEEECCCCCEEEEEEEECCCCCEEHHHCCCHHHHHHHHCCCCCCCCEEHHHHHH
VWNTMKTIPHKAASR
HHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA