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The map label for this gene is uvrB

Identifier: 116327526

GI number: 116327526

Start: 856550

End: 858550

Strand: Reverse

Name: uvrB

Synonym: LBL_0755

Alternate gene names: 116327526

Gene position: 858550-856550 (Counterclockwise)

Preceding gene: 116327527

Following gene: 116327525

Centisome position: 23.75

GC content: 43.23

Gene sequence:

>2001_bases
ATGGCTTCTATTTTTAAAATTCACTCCGCATACAAACCCGCCGGAGATCAGATTAAGGCGATCGAAAATATCGCGGACTC
CTTTCAAAAAGGCGCTGAAAAAGTCACACTGGTCGGAGTAACCGGCTCCGGTAAAACTTTCACGATGGCTCAGGTGATTC
AAAATCTCGGACTTCCGACCTTAGTCTTGTCGCATAACAAAACCTTGGCGGCCCAACTTTTCCGGGAGTTTAAAGAATTT
TTTCCCGAGAATGCGGTGGAATATTTCGTTTCCTATTATGATTATTATCAGCCGGAAGCATACGTTCCTTCTTCGGATAC
GTTTATCGAAAAAGATAGTTCCATCAACGAAGAGATCGACAAACTCAGGCTTAGGGCCACGTCTTCTCTTTTAGAAAGAG
AAGACGTCGTCATTGTCAGTTCGGTTTCCTGTATTTACGGTTTAGGTTCTCCGGAAGAATATACGAATTCGGTGGTCGCT
TTAAAAACGGGGGACACGATCGAAAGGGATGCGGTAATCCGCAAACTGTTGCATATTCAATATAATAGAAACGACATAGA
CTTTTCCAGAGGGAACTTCAGAGTTCGAGGGGATTCGATCGAAATTTATCCCGCGTATCACACAGACGGAATCCGAATCG
AATTTTTCGGAGACGAGATCGACTCGATCAGTAGAATCAATCCGATAACCGCACAAACGATTCTTAAATTAGAAAAGACT
TATATTTATCCCGCAAAACACTTCATCACTTCCGGCCCGAAAGTAAAGGTGGCGATCGAAAACATCAAAGCGGAACTGGA
AGCCCAAGCTCTTTTTTTTAGAAAAAACGACAAACTTTTGGAAGCGGAAAGAATTATATCCCGCACGAATTACGATATGG
AAATGCTTCAGGAGATGGGATACTGTAACGGGATCGAAAATTATTCGAGACATCTCACCGGAAGAAAAGCGGGAGAACGT
CCGGCTTGTTTGATCGATTATTTTCAAGGAGAATTTCTTTTGATTGTAGACGAGTCTCATGTGACGATTCCTCAAATCGG
TGGAATGTTCGCGGGAGACAAGGCACGTAAACAAACATTAGTAGACTTCGGTTTTAGACTTCCAAGTGCGCTCGATAATA
GACCTCTCAATTTTGAAGAGTTCGAAACTTTGACTCCGAAAACTCTCTATGTTTCCGCGACTCCCGCAGATTACGAAATG
GAAAAAAGTTCCAAAGTCGTGGAACAAATCATCCGACCAACCGGACTTTTGGATCCGATCGTGGAAGTGCGTTCCACCAA
AAATCAAATCGAGGACCTTCTTGTGGAAATCCGCAAACGAATCGATGTAGGCGAACGCATCCTTATCACGACACTGACTA
AAAAAATGTCGGAAGATCTTACGGACTATTACAAAGAAATCGGCCTGCAAGTCGCGTATCTTCATTCCGAGGTAGAAACT
TTGGATCGGGTCGCCATCATTCGAGACCTGAGAAAAGGTATCTACGACGTTTTGATCGGAATCAATCTTTTACGGGAAGG
TCTGGATATTCCCGAGGTTTCGTTAGTCGCGATTTTGGACGCGGATAAGGAAGGTTTTTTAAGAAATTATAAATCCCTAA
TACAGACTGTCGGTCGTGCCGCGAGAAACGTAAACGGAACTGCGATCCTTTACGCGGACAAGATCACGGACTCGATGGCA
AAAGCGATCGATGAAACGAAACGCAGAAGAAAGATCCAGGAAGACCATAATCTCAAATTCGGAATCACCCCTCTTACAAT
CCGAAAAGAAGTCAACGACATTATCGAACGAGAGGAAAAGAAAAGGACTTCGGAAGATCTGGTTCTTGAAGACGTGGAGA
AAAAATTTAATTCCAAGAAGTTCCCGAACAAGGAAGTTTTGAAGGATAAACTCCGAGAAGAAATGATGAAGGCCGCAAAA
GAACTCGACTTTGAAAGAGCCGCCATCCTAAGAGATAAGATGTTATCCATCCAAATAAACGACCCTTCGACTGAAAACTA
A

Upstream 100 bases:

>100_bases
TGATGGTCATGCAAAAACTTTTTGACGAACTCGATCTTGAAATTATAGAGGGTAAAAAACAAGCTCAGTTCAACGCCAAG
AAAAAACTGTCCTAGTCCTT

Downstream 100 bases:

>100_bases
CACAAAGGAATCTATTTTGATCACAATACTGATTTGTCTCGTCACCTTCGGTTTAAGTATTTGGTGTTTTGCCTCCGAGG
ATAAACTCGGTAAGTTCGTT

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B

Number of amino acids: Translated: 666; Mature: 665

Protein sequence:

>666_residues
MASIFKIHSAYKPAGDQIKAIENIADSFQKGAEKVTLVGVTGSGKTFTMAQVIQNLGLPTLVLSHNKTLAAQLFREFKEF
FPENAVEYFVSYYDYYQPEAYVPSSDTFIEKDSSINEEIDKLRLRATSSLLEREDVVIVSSVSCIYGLGSPEEYTNSVVA
LKTGDTIERDAVIRKLLHIQYNRNDIDFSRGNFRVRGDSIEIYPAYHTDGIRIEFFGDEIDSISRINPITAQTILKLEKT
YIYPAKHFITSGPKVKVAIENIKAELEAQALFFRKNDKLLEAERIISRTNYDMEMLQEMGYCNGIENYSRHLTGRKAGER
PACLIDYFQGEFLLIVDESHVTIPQIGGMFAGDKARKQTLVDFGFRLPSALDNRPLNFEEFETLTPKTLYVSATPADYEM
EKSSKVVEQIIRPTGLLDPIVEVRSTKNQIEDLLVEIRKRIDVGERILITTLTKKMSEDLTDYYKEIGLQVAYLHSEVET
LDRVAIIRDLRKGIYDVLIGINLLREGLDIPEVSLVAILDADKEGFLRNYKSLIQTVGRAARNVNGTAILYADKITDSMA
KAIDETKRRRKIQEDHNLKFGITPLTIRKEVNDIIEREEKKRTSEDLVLEDVEKKFNSKKFPNKEVLKDKLREEMMKAAK
ELDFERAAILRDKMLSIQINDPSTEN

Sequences:

>Translated_666_residues
MASIFKIHSAYKPAGDQIKAIENIADSFQKGAEKVTLVGVTGSGKTFTMAQVIQNLGLPTLVLSHNKTLAAQLFREFKEF
FPENAVEYFVSYYDYYQPEAYVPSSDTFIEKDSSINEEIDKLRLRATSSLLEREDVVIVSSVSCIYGLGSPEEYTNSVVA
LKTGDTIERDAVIRKLLHIQYNRNDIDFSRGNFRVRGDSIEIYPAYHTDGIRIEFFGDEIDSISRINPITAQTILKLEKT
YIYPAKHFITSGPKVKVAIENIKAELEAQALFFRKNDKLLEAERIISRTNYDMEMLQEMGYCNGIENYSRHLTGRKAGER
PACLIDYFQGEFLLIVDESHVTIPQIGGMFAGDKARKQTLVDFGFRLPSALDNRPLNFEEFETLTPKTLYVSATPADYEM
EKSSKVVEQIIRPTGLLDPIVEVRSTKNQIEDLLVEIRKRIDVGERILITTLTKKMSEDLTDYYKEIGLQVAYLHSEVET
LDRVAIIRDLRKGIYDVLIGINLLREGLDIPEVSLVAILDADKEGFLRNYKSLIQTVGRAARNVNGTAILYADKITDSMA
KAIDETKRRRKIQEDHNLKFGITPLTIRKEVNDIIEREEKKRTSEDLVLEDVEKKFNSKKFPNKEVLKDKLREEMMKAAK
ELDFERAAILRDKMLSIQINDPSTEN
>Mature_665_residues
ASIFKIHSAYKPAGDQIKAIENIADSFQKGAEKVTLVGVTGSGKTFTMAQVIQNLGLPTLVLSHNKTLAAQLFREFKEFF
PENAVEYFVSYYDYYQPEAYVPSSDTFIEKDSSINEEIDKLRLRATSSLLEREDVVIVSSVSCIYGLGSPEEYTNSVVAL
KTGDTIERDAVIRKLLHIQYNRNDIDFSRGNFRVRGDSIEIYPAYHTDGIRIEFFGDEIDSISRINPITAQTILKLEKTY
IYPAKHFITSGPKVKVAIENIKAELEAQALFFRKNDKLLEAERIISRTNYDMEMLQEMGYCNGIENYSRHLTGRKAGERP
ACLIDYFQGEFLLIVDESHVTIPQIGGMFAGDKARKQTLVDFGFRLPSALDNRPLNFEEFETLTPKTLYVSATPADYEME
KSSKVVEQIIRPTGLLDPIVEVRSTKNQIEDLLVEIRKRIDVGERILITTLTKKMSEDLTDYYKEIGLQVAYLHSEVETL
DRVAIIRDLRKGIYDVLIGINLLREGLDIPEVSLVAILDADKEGFLRNYKSLIQTVGRAARNVNGTAILYADKITDSMAK
AIDETKRRRKIQEDHNLKFGITPLTIRKEVNDIIEREEKKRTSEDLVLEDVEKKFNSKKFPNKEVLKDKLREEMMKAAKE
LDFERAAILRDKMLSIQINDPSTEN

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain

Homologues:

Organism=Escherichia coli, GI1786996, Length=665, Percent_Identity=56.6917293233083, Blast_Score=746, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UVRB_LEPBJ (Q04QJ9)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801579.1
- ProteinModelPortal:   Q04QJ9
- SMR:   Q04QJ9
- STRING:   Q04QJ9
- GeneID:   4410451
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2353
- eggNOG:   COG0556
- HOGENOM:   HBG703949
- OMA:   AAQLCNE
- PhylomeDB:   Q04QJ9
- ProtClustDB:   PRK05298
- BioCyc:   LBOR355277:LBJ_2353-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00204
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00631

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR; SSF46600 UvrB_C

EC number: NA

Molecular weight: Translated: 75951; Mature: 75820

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIFKIHSAYKPAGDQIKAIENIADSFQKGAEKVTLVGVTGSGKTFTMAQVIQNLGLPT
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEHHHHHHHHCCCCE
LVLSHNKTLAAQLFREFKEFFPENAVEYFVSYYDYYQPEAYVPSSDTFIEKDSSINEEID
EEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHH
KLRLRATSSLLEREDVVIVSSVSCIYGLGSPEEYTNSVVALKTGDTIERDAVIRKLLHIQ
HHHHHHHHHHHCCCCEEEEEECHHHHCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHE
YNRNDIDFSRGNFRVRGDSIEIYPAYHTDGIRIEFFGDEIDSISRINPITAQTILKLEKT
ECCCCCCCCCCCEEEECCCEEEEEEECCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHH
YIYPAKHFITSGPKVKVAIENIKAELEAQALFFRKNDKLLEAERIISRTNYDMEMLQEMG
EECCHHHHHCCCCEEEEEHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCHHHHHHCC
YCNGIENYSRHLTGRKAGERPACLIDYFQGEFLLIVDESHVTIPQIGGMFAGDKARKQTL
CCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCEEHHCCCCCCCCCHHHHHHH
VDFGFRLPSALDNRPLNFEEFETLTPKTLYVSATPADYEMEKSSKVVEQIIRPTGLLDPI
HHHHCCCCHHHCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHH
VEVRSTKNQIEDLLVEIRKRIDVGERILITTLTKKMSEDLTDYYKEIGLQVAYLHSEVET
HHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHH
LDRVAIIRDLRKGIYDVLIGINLLREGLDIPEVSLVAILDADKEGFLRNYKSLIQTVGRA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHH
ARNVNGTAILYADKITDSMAKAIDETKRRRKIQEDHNLKFGITPLTIRKEVNDIIEREEK
HCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHH
KRTSEDLVLEDVEKKFNSKKFPNKEVLKDKLREEMMKAAKELDFERAAILRDKMLSIQIN
HCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEEEEC
DPSTEN
CCCCCC
>Mature Secondary Structure 
ASIFKIHSAYKPAGDQIKAIENIADSFQKGAEKVTLVGVTGSGKTFTMAQVIQNLGLPT
CCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEHHHHHHHHCCCCE
LVLSHNKTLAAQLFREFKEFFPENAVEYFVSYYDYYQPEAYVPSSDTFIEKDSSINEEID
EEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHH
KLRLRATSSLLEREDVVIVSSVSCIYGLGSPEEYTNSVVALKTGDTIERDAVIRKLLHIQ
HHHHHHHHHHHCCCCEEEEEECHHHHCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHE
YNRNDIDFSRGNFRVRGDSIEIYPAYHTDGIRIEFFGDEIDSISRINPITAQTILKLEKT
ECCCCCCCCCCCEEEECCCEEEEEEECCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHH
YIYPAKHFITSGPKVKVAIENIKAELEAQALFFRKNDKLLEAERIISRTNYDMEMLQEMG
EECCHHHHHCCCCEEEEEHHHHHHHHHHHHHEEECCCCHHHHHHHHHHCCCCHHHHHHCC
YCNGIENYSRHLTGRKAGERPACLIDYFQGEFLLIVDESHVTIPQIGGMFAGDKARKQTL
CCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCEEHHCCCCCCCCCHHHHHHH
VDFGFRLPSALDNRPLNFEEFETLTPKTLYVSATPADYEMEKSSKVVEQIIRPTGLLDPI
HHHHCCCCHHHCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHH
VEVRSTKNQIEDLLVEIRKRIDVGERILITTLTKKMSEDLTDYYKEIGLQVAYLHSEVET
HHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHH
LDRVAIIRDLRKGIYDVLIGINLLREGLDIPEVSLVAILDADKEGFLRNYKSLIQTVGRA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHH
ARNVNGTAILYADKITDSMAKAIDETKRRRKIQEDHNLKFGITPLTIRKEVNDIIEREEK
HCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHH
KRTSEDLVLEDVEKKFNSKKFPNKEVLKDKLREEMMKAAKELDFERAAILRDKMLSIQIN
HCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEEEEC
DPSTEN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA