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The map label for this gene is gst-1 [H]

Identifier: 116327436

GI number: 116327436

Start: 723171

End: 723827

Strand: Reverse

Name: gst-1 [H]

Synonym: LBL_0650

Alternate gene names: 116327436

Gene position: 723827-723171 (Counterclockwise)

Preceding gene: 116327437

Following gene: 116327430

Centisome position: 20.03

GC content: 40.79

Gene sequence:

>657_bases
TTGATCGAGTTATATTCTGCTTCCACACCCAACGGAAGAAAAGTTTCCATCATGTTAGAAGAATTAGGAATTCCTTATAC
GATTCATCCGATTGATCTGGACAAACTGGAGCAAAAACAAGAGTGGTTTTTGAAAATTAATCCGAACGGTAGAATCCCTG
CGATTATCGACACGGACAATGGAAACTTTGCCGTGTTCGAATCGGGAGCGATTCTGATCTATCTCGCGGAAAAAACCGGT
AAATTTTTACCGAAAGATCCAAAGGAAAAAAGTATCGCACTACAATGGCTTATGTTTCAGATGGGCGGGGTCGGTCCGAT
GCAAGGACAAGCCGGAGTATTCTTAAAATACGCTCCCGAAAAGATTCCTTTCGCGATTTCCAGATATCAAAACGAAACAA
AACGTCTGTATTCCGTTTTGGATAGAAGACTCGGTGAAAGTCAATTTTTAGCCGGAAAAGAATTATCGATCGCCGATATC
GCAACCTGGCCTTGGGTCAACATACACGATTGGGTAGAAATTTCTCTGGATGAATTTCCGAATTTAAAACGTTGGAACGA
AGAACTCGCAGAACGCCCGGCTTTCATCAAAGGAAAAAACATTCCGAACAAAACGAATCATAAAAATAACACGGAAGAGA
TAAAAAAGAAAGTTTAG

Upstream 100 bases:

>100_bases
AAAAATCGACTTCGGAATTTTAACTTTTTTATTTACATTTCAAAAAGGATAAAAGTCTAATCCCCAAAATAAGTAATTTA
AAAATTTTAGGAGAAAACCT

Downstream 100 bases:

>100_bases
GTCACACTAAGAAAGTAGAATTTTCTAATTTTTCCGGAGACGGTTCGGCAACGGGAAGGATCACGAAAAAAGTGGTCCCC
TGTTCCGAAGTTTCAAAAAA

Product: glutathione transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MIELYSASTPNGRKVSIMLEELGIPYTIHPIDLDKLEQKQEWFLKINPNGRIPAIIDTDNGNFAVFESGAILIYLAEKTG
KFLPKDPKEKSIALQWLMFQMGGVGPMQGQAGVFLKYAPEKIPFAISRYQNETKRLYSVLDRRLGESQFLAGKELSIADI
ATWPWVNIHDWVEISLDEFPNLKRWNEELAERPAFIKGKNIPNKTNHKNNTEEIKKKV

Sequences:

>Translated_218_residues
MIELYSASTPNGRKVSIMLEELGIPYTIHPIDLDKLEQKQEWFLKINPNGRIPAIIDTDNGNFAVFESGAILIYLAEKTG
KFLPKDPKEKSIALQWLMFQMGGVGPMQGQAGVFLKYAPEKIPFAISRYQNETKRLYSVLDRRLGESQFLAGKELSIADI
ATWPWVNIHDWVEISLDEFPNLKRWNEELAERPAFIKGKNIPNKTNHKNNTEEIKKKV
>Mature_218_residues
MIELYSASTPNGRKVSIMLEELGIPYTIHPIDLDKLEQKQEWFLKINPNGRIPAIIDTDNGNFAVFESGAILIYLAEKTG
KFLPKDPKEKSIALQWLMFQMGGVGPMQGQAGVFLKYAPEKIPFAISRYQNETKRLYSVLDRRLGESQFLAGKELSIADI
ATWPWVNIHDWVEISLDEFPNLKRWNEELAERPAFIKGKNIPNKTNHKNNTEEIKKKV

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=198, Percent_Identity=51.010101010101, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI87082195, Length=219, Percent_Identity=42.4657534246575, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI87081789, Length=165, Percent_Identity=30.9090909090909, Blast_Score=71, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324100, Length=230, Percent_Identity=35.6521739130435, Blast_Score=131, Evalue=9e-32,
Organism=Drosophila melanogaster, GI17864596, Length=188, Percent_Identity=30.3191489361702, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17864594, Length=190, Percent_Identity=31.5789473684211, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17933730, Length=189, Percent_Identity=30.1587301587302, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17864598, Length=190, Percent_Identity=30, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45549270, Length=187, Percent_Identity=29.9465240641711, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864592, Length=181, Percent_Identity=30.939226519337, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI85725204, Length=192, Percent_Identity=30.7291666666667, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17737923, Length=192, Percent_Identity=30.7291666666667, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571670, Length=171, Percent_Identity=32.1637426900585, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922160, Length=200, Percent_Identity=27, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24646251, Length=187, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221378141, Length=193, Percent_Identity=27.979274611399, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20130115, Length=192, Percent_Identity=26.0416666666667, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI62472445, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922932, Length=197, Percent_Identity=26.9035532994924, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24654965, Length=198, Percent_Identity=25.7575757575758, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922526, Length=206, Percent_Identity=27.6699029126214, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 24972; Mature: 24972

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELYSASTPNGRKVSIMLEELGIPYTIHPIDLDKLEQKQEWFLKINPNGRIPAIIDTDN
CEEEECCCCCCCCEEEEEEHHHCCCEEEECCCHHHHCCCCEEEEEECCCCCCCEEEECCC
GNFAVFESGAILIYLAEKTGKFLPKDPKEKSIALQWLMFQMGGVGPMQGQAGVFLKYAPE
CCEEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
KIPFAISRYQNETKRLYSVLDRRLGESQFLAGKELSIADIATWPWVNIHDWVEISLDEFP
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEHHHCC
NLKRWNEELAERPAFIKGKNIPNKTNHKNNTEEIKKKV
CHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MIELYSASTPNGRKVSIMLEELGIPYTIHPIDLDKLEQKQEWFLKINPNGRIPAIIDTDN
CEEEECCCCCCCCEEEEEEHHHCCCEEEECCCHHHHCCCCEEEEEECCCCCCCEEEECCC
GNFAVFESGAILIYLAEKTGKFLPKDPKEKSIALQWLMFQMGGVGPMQGQAGVFLKYAPE
CCEEEEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
KIPFAISRYQNETKRLYSVLDRRLGESQFLAGKELSIADIATWPWVNIHDWVEISLDEFP
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEHHHCC
NLKRWNEELAERPAFIKGKNIPNKTNHKNNTEEIKKKV
CHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]