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The map label for this gene is amiA [H]

Identifier: 116327339

GI number: 116327339

Start: 587912

End: 589003

Strand: Reverse

Name: amiA [H]

Synonym: LBL_0535

Alternate gene names: 116327339

Gene position: 589003-587912 (Counterclockwise)

Preceding gene: 116327340

Following gene: 116327338

Centisome position: 16.3

GC content: 41.58

Gene sequence:

>1092_bases
TTGGCAAAAAATCAAATCTATTTTTGGACGCTGATCTTATTTTTTCCAAACGCGTGGGAGTTAGAAGCTAAAATTTCCAT
TCCCACTCAATCCTCTAAACATTATGTGCACTTTGAGGACATACAAAAAGAATTTCCTTCTCTCAAATCCTCCTTTAATC
CCGCTACCTTCGTAGGGGCGATCCAACATCCTTCCGGGGAAGTTCGTTTTAGAGTCGGCTCCTCCTTTTACACCTTTAAT
CAAACTATAGAAAAAACTTCCGTTCCGGTTTTATACAAGAAAAAGGATTTTCTGATCCCTCCCGAAATCGTAGAAGCGAT
TTTCGTCCAACTCATGTCGGACGATATCCGCTACGAATATAAGGAAAACGTATTGGAATTGGAGATTTTACCGAGTACTG
AAAAACTGGAAATCAAAACAATTCTCATCGATGCGGGACACGGAGGAAAAGATCCGGGAACGGCGTCAACCGACGGAACC
AATGAAAAGTTATTAGCTTTACAAGTCGCCAAAATTCTTCAGAAATTTTTCGAGAAAGTATATCCGACAATTAATGTCGT
ATTAACGAGATCAGACGATACGTTTATCGAATTAGAACGAAGATCCGAAATCGCAAACCGGGAACTGAAAAAAAGCGGAA
GCGCGTTGTTCATCAGTCTACACTGTAATTCTTCGATCAACGTGGACGTGAACGGATTCGAGATCTATTATCTTTCTCAA
ACTCCATCCACCGAGTCGGCTCGTGAGACCGCTCTTCTGGAAAACAGGATTTTTAAACCGAAAGGGTCCCCGGCCATCAA
AAAAGTCCAGGCAGGGATGATGTCCTCTCTGATTCAAAGAAGAAGTAGAATCTTGGCCAGGTCCTTGGAATCGGAGATGA
AAAAAAAGCTTCAACCTCAAATCCTGTCCAGGGGAGTGAAAAAAGCGGATTTTTCGGTCCTCAGAGGAAGCCTCATGCCT
GCGGTTCTTGTGGAAATGGGGTACCTTTCTCATGAAAAAGAATCCAAACTTTTGCAGAGTAAAAGTTTACAAGTTAAGAT
AGCGAAAAGCATCGTAGAAGGAATTCGAGGATATGAATTGGCAAAAAATTAA

Upstream 100 bases:

>100_bases
ACAAACAACACGTGAACCAAAATTACGACAAGAGTAAACGAAATCTTTTCGATATCAACGATACCGTAAGAGAAGACACC
AAAAAGAAAAAGGGTTCGAT

Downstream 100 bases:

>100_bases
AAAACCTGCGATCGCAATCAGAGACGCAATTTGGAAAAAGATGAAAGTCGCGGGAGAAAAAATCAATCAAGGATATCTTT
GGCTTTTTCGTATCGCGACC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAKNQIYFWTLILFFPNAWELEAKISIPTQSSKHYVHFEDIQKEFPSLKSSFNPATFVGAIQHPSGEVRFRVGSSFYTFN
QTIEKTSVPVLYKKKDFLIPPEIVEAIFVQLMSDDIRYEYKENVLELEILPSTEKLEIKTILIDAGHGGKDPGTASTDGT
NEKLLALQVAKILQKFFEKVYPTINVVLTRSDDTFIELERRSEIANRELKKSGSALFISLHCNSSINVDVNGFEIYYLSQ
TPSTESARETALLENRIFKPKGSPAIKKVQAGMMSSLIQRRSRILARSLESEMKKKLQPQILSRGVKKADFSVLRGSLMP
AVLVEMGYLSHEKESKLLQSKSLQVKIAKSIVEGIRGYELAKN

Sequences:

>Translated_363_residues
MAKNQIYFWTLILFFPNAWELEAKISIPTQSSKHYVHFEDIQKEFPSLKSSFNPATFVGAIQHPSGEVRFRVGSSFYTFN
QTIEKTSVPVLYKKKDFLIPPEIVEAIFVQLMSDDIRYEYKENVLELEILPSTEKLEIKTILIDAGHGGKDPGTASTDGT
NEKLLALQVAKILQKFFEKVYPTINVVLTRSDDTFIELERRSEIANRELKKSGSALFISLHCNSSINVDVNGFEIYYLSQ
TPSTESARETALLENRIFKPKGSPAIKKVQAGMMSSLIQRRSRILARSLESEMKKKLQPQILSRGVKKADFSVLRGSLMP
AVLVEMGYLSHEKESKLLQSKSLQVKIAKSIVEGIRGYELAKN
>Mature_362_residues
AKNQIYFWTLILFFPNAWELEAKISIPTQSSKHYVHFEDIQKEFPSLKSSFNPATFVGAIQHPSGEVRFRVGSSFYTFNQ
TIEKTSVPVLYKKKDFLIPPEIVEAIFVQLMSDDIRYEYKENVLELEILPSTEKLEIKTILIDAGHGGKDPGTASTDGTN
EKLLALQVAKILQKFFEKVYPTINVVLTRSDDTFIELERRSEIANRELKKSGSALFISLHCNSSINVDVNGFEIYYLSQT
PSTESARETALLENRIFKPKGSPAIKKVQAGMMSSLIQRRSRILARSLESEMKKKLQPQILSRGVKKADFSVLRGSLMPA
VLVEMGYLSHEKESKLLQSKSLQVKIAKSIVEGIRGYELAKN

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=32.4444444444444, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790611, Length=236, Percent_Identity=31.7796610169492, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082163, Length=227, Percent_Identity=28.6343612334802, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 41077; Mature: 40946

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNQIYFWTLILFFPNAWELEAKISIPTQSSKHYVHFEDIQKEFPSLKSSFNPATFVGA
CCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEE
IQHPSGEVRFRVGSSFYTFNQTIEKTSVPVLYKKKDFLIPPEIVEAIFVQLMSDDIRYEY
EECCCCCEEEEECCEEEEHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
KENVLELEILPSTEKLEIKTILIDAGHGGKDPGTASTDGTNEKLLALQVAKILQKFFEKV
HCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YPTINVVLTRSDDTFIELERRSEIANRELKKSGSALFISLHCNSSINVDVNGFEIYYLSQ
CCEEEEEEECCCCCEEEEHHHHHHHHHHHHCCCCEEEEEEEECCEEEEEECCEEEEEEEC
TPSTESARETALLENRIFKPKGSPAIKKVQAGMMSSLIQRRSRILARSLESEMKKKLQPQ
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ILSRGVKKADFSVLRGSLMPAVLVEMGYLSHEKESKLLQSKSLQVKIAKSIVEGIRGYEL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
AKN
CCC
>Mature Secondary Structure 
AKNQIYFWTLILFFPNAWELEAKISIPTQSSKHYVHFEDIQKEFPSLKSSFNPATFVGA
CCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEE
IQHPSGEVRFRVGSSFYTFNQTIEKTSVPVLYKKKDFLIPPEIVEAIFVQLMSDDIRYEY
EECCCCCEEEEECCEEEEHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
KENVLELEILPSTEKLEIKTILIDAGHGGKDPGTASTDGTNEKLLALQVAKILQKFFEKV
HCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YPTINVVLTRSDDTFIELERRSEIANRELKKSGSALFISLHCNSSINVDVNGFEIYYLSQ
CCEEEEEEECCCCCEEEEHHHHHHHHHHHHCCCCEEEEEEEECCEEEEEECCEEEEEEEC
TPSTESARETALLENRIFKPKGSPAIKKVQAGMMSSLIQRRSRILARSLESEMKKKLQPQ
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ILSRGVKKADFSVLRGSLMPAVLVEMGYLSHEKESKLLQSKSLQVKIAKSIVEGIRGYEL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
AKN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]