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The map label for this gene is ispE [H]

Identifier: 116327333

GI number: 116327333

Start: 579731

End: 580624

Strand: Reverse

Name: ispE [H]

Synonym: LBL_0528

Alternate gene names: 116327333

Gene position: 580624-579731 (Counterclockwise)

Preceding gene: 116327334

Following gene: 116327332

Centisome position: 16.06

GC content: 41.39

Gene sequence:

>894_bases
TTGATTTCTCCGGCTAAAATCAATCTCGGTTTGGAAATTCCGTTTAAACGTCCGGACGGGTTTCATGAAATTCGAAGTAT
ATTTCTAAAAATCTCTTGGGGAGACGATATCGAAATTGAACCAGCGGATAACGGAGTTTTCGAACTTTTTTCTGAAAACG
AAATCATATTAGAAAAACGAAAACTATACGATCAGGTTTCAGAGATAGGCGACATCAAAAAAAATATTCTTTATAAAACT
TTTATAAAGGCAAGATCCCTTTTTCCGGAACTTCCAGGAGTAAAAATCCATCTTACAAAAAGAATTTCTCCGGCTGGGGG
CCTCGGAGGAGGAAGTACAAACGCGGCTTCTTTATTAAACTTTCTTTTTTCTTGGCGTTCCTTTTTTACCTCCGACGAAA
TGCGCGTATTTGCGACCGAAATCGGTTCGGATGTTCCTTTTTTCCTAGGAGAAGGACATGCATTCGTTACCGGAAAGGGA
GAAATTTCGGAAGAGATCGAGGTTCATCCCGGCCAAGGAATCCTTGCTTTAACTCCGCAAGTGATGAATACGGCGGAAAT
GTATACCTTGTTGAAAAAACCTTTACAAGAGAGTGCTTCTCAGAAAAATGGAAACACTCTGTCGGAAAGTCTGATTTCTG
TTCTAAAAAGCGGAGACTGGAGATCTCTACAGGGTAGGCTCTTGAATGATTTTGAGCCGGTTGCCTTCCAACTTCATCCG
GAATTGGGAGTTCTCAAGGACAAATTCCTGGAGTTTGGATCCAGTTATTGTTCTTTGACCGGTTCAGGTTCAAGTATGTA
CGGGCTGGTTCAGGGCCTTGAGATCCAAGAAGAACTGTTGCACAGACTGAGGCAGGAATTCTCAAATCTCACATTCGTAC
GATTTAATTTTTAG

Upstream 100 bases:

>100_bases
ATCAGCTTTCTCTAAAGCAAACAACGTTGAGATTCCATTTTGAATTTTCGGTTGGAGCAAAAGGAAGCGTAGTGTTTCAT
GAACTTATAGGGAGTCGTCT

Downstream 100 bases:

>100_bases
AAACTGGGCTGTCGCCAAGTGGTAAGGCAGCGGTTTTTGGTATCGCCATTTCCTAGGTTCGAATCCTAGCAGCCCAGCCA
TTGAATTACTGACATTGATT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MISPAKINLGLEIPFKRPDGFHEIRSIFLKISWGDDIEIEPADNGVFELFSENEIILEKRKLYDQVSEIGDIKKNILYKT
FIKARSLFPELPGVKIHLTKRISPAGGLGGGSTNAASLLNFLFSWRSFFTSDEMRVFATEIGSDVPFFLGEGHAFVTGKG
EISEEIEVHPGQGILALTPQVMNTAEMYTLLKKPLQESASQKNGNTLSESLISVLKSGDWRSLQGRLLNDFEPVAFQLHP
ELGVLKDKFLEFGSSYCSLTGSGSSMYGLVQGLEIQEELLHRLRQEFSNLTFVRFNF

Sequences:

>Translated_297_residues
MISPAKINLGLEIPFKRPDGFHEIRSIFLKISWGDDIEIEPADNGVFELFSENEIILEKRKLYDQVSEIGDIKKNILYKT
FIKARSLFPELPGVKIHLTKRISPAGGLGGGSTNAASLLNFLFSWRSFFTSDEMRVFATEIGSDVPFFLGEGHAFVTGKG
EISEEIEVHPGQGILALTPQVMNTAEMYTLLKKPLQESASQKNGNTLSESLISVLKSGDWRSLQGRLLNDFEPVAFQLHP
ELGVLKDKFLEFGSSYCSLTGSGSSMYGLVQGLEIQEELLHRLRQEFSNLTFVRFNF
>Mature_297_residues
MISPAKINLGLEIPFKRPDGFHEIRSIFLKISWGDDIEIEPADNGVFELFSENEIILEKRKLYDQVSEIGDIKKNILYKT
FIKARSLFPELPGVKIHLTKRISPAGGLGGGSTNAASLLNFLFSWRSFFTSDEMRVFATEIGSDVPFFLGEGHAFVTGKG
EISEEIEVHPGQGILALTPQVMNTAEMYTLLKKPLQESASQKNGNTLSESLISVLKSGDWRSLQGRLLNDFEPVAFQLHP
ELGVLKDKFLEFGSSYCSLTGSGSSMYGLVQGLEIQEELLHRLRQEFSNLTFVRFNF

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=275, Percent_Identity=29.8181818181818, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 33216; Mature: 33216

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPAKINLGLEIPFKRPDGFHEIRSIFLKISWGDDIEIEPADNGVFELFSENEIILEKR
CCCCEEEEEEEEECCCCCCCHHHHHHEEEEEECCCCEEEEECCCCCEEEECCCHHHHHHH
KLYDQVSEIGDIKKNILYKTFIKARSLFPELPGVKIHLTKRISPAGGLGGGSTNAASLLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHH
FLFSWRSFFTSDEMRVFATEIGSDVPFFLGEGHAFVTGKGEISEEIEVHPGQGILALTPQ
HHHHHHHHCCCCCCEEEEHHHCCCCCEEEECCEEEEECCCCCCCCEEECCCCCEEEECHH
VMNTAEMYTLLKKPLQESASQKNGNTLSESLISVLKSGDWRSLQGRLLNDFEPVAFQLHP
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEECC
ELGVLKDKFLEFGSSYCSLTGSGSSMYGLVQGLEIQEELLHRLRQEFSNLTFVRFNF
CHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MISPAKINLGLEIPFKRPDGFHEIRSIFLKISWGDDIEIEPADNGVFELFSENEIILEKR
CCCCEEEEEEEEECCCCCCCHHHHHHEEEEEECCCCEEEEECCCCCEEEECCCHHHHHHH
KLYDQVSEIGDIKKNILYKTFIKARSLFPELPGVKIHLTKRISPAGGLGGGSTNAASLLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHH
FLFSWRSFFTSDEMRVFATEIGSDVPFFLGEGHAFVTGKGEISEEIEVHPGQGILALTPQ
HHHHHHHHCCCCCCEEEEHHHCCCCCEEEECCEEEEECCCCCCCCEEECCCCCEEEECHH
VMNTAEMYTLLKKPLQESASQKNGNTLSESLISVLKSGDWRSLQGRLLNDFEPVAFQLHP
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEECC
ELGVLKDKFLEFGSSYCSLTGSGSSMYGLVQGLEIQEELLHRLRQEFSNLTFVRFNF
CHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA