Click here to switch to the map view.

The map label for this gene is pntB [H]

Identifier: 116327177

GI number: 116327177

Start: 415982

End: 417382

Strand: Reverse

Name: pntB [H]

Synonym: LBL_0356

Alternate gene names: 116327177

Gene position: 417382-415982 (Counterclockwise)

Preceding gene: 116327178

Following gene: 116327176

Centisome position: 11.55

GC content: 47.47

Gene sequence:

>1401_bases
ATGGAAAAGTCGATCATCAATTTAGTTTATCTTCTATCCTCGATCCTTTTTATCGTAGGATTAAAACTTCTTTCTCACCC
AAAGACCGCAGTCAAAGGAAACTTTACAGGCGCTGTGGGAATGTTCTTCGCGATAGCGGCGGCCCTTATTGAAAAAGGTC
TTGCATACGAGTATATCCTAGCGGGATTCATCGTCGGAACCGCAATCGGAGTTTATCTCTCCGTGAAAGTGGAAATGACC
GCAATGCCTCAACTTGTCGCGGCATTGAACGGATTCGGAGGACTTGCCTCCTTTCTCGTTGCAGGCGCCGCAGTGATGGA
AGTTCTACATCAAGGAACCAACCTCGCAGTTTTAAAATCGTATCAATTTACGATTTCCACCGCCGCTTCCGGAATTATCG
GAGCCGTGACTCTTACTGGAAGTTTTGTGGCTTATGGAAAACTCCAGGGAATTCTTTCCGAAAAAGCGGTTCGTTATCCG
GGCGATCAGCTTGTAAAAATTCTTTTCTTTCTCGGTTCCATCGCTCTCAGCTACTTTGTTGTGATTCAACCTGAAAAGAT
AGAATGGTATTGGTATGTAGTCGTAGCGGGTTCCATTCTAGGAATTTTACTCGTAATGCCGATCGGCGGAGCGGATATGC
CCGTCGTCATCGCACTTTTGAACTCTTATTCGGGAATCGCGGCTTCCGCCACCGGATTTGTCCTCGGCAACAACGTTCTC
ATCATCGCGGGTTCTCTCGTGGGAGCTTCCGGAATTATCCTAACACAAATTATGTGCAAGGCAATGAACCGTTCTCTTCC
GAATGTTCTTTTCGGTGGACTCGGCGCCGCTGTAGACAACTCCAAAGGCGGAGAAGACATCTATGCAGGAAAAACCAAAA
GTACTTCCGCAGAAGAAGTTGCGATGCTTTTGGATATGGCCCAGAGAGTCGTGATCGTTCCCGGCTACGGAATGGCGGTT
GCACAGGCTCAACATGCCGTAAGAGATCTTTATAATATTCTAACGGATAAAGGAATCGACGTTGAATTTGCGATTCATCC
CGTTGCGGGAAGAATGCCCGGTCACATGAACGTTCTCTTGGCGGAAGCCGACATCCCTTACGACAAGATGAAAGAAATGG
ACGAGATCAATCCTTCTTTCGAACAAGTCGATGTGGTGATCGTCAACGGCGCCAACGACGTGGTAAATCCCCTTGCTAAA
ACGGATCCGAGTAGCCCGATCGCGGGAATGCCGATTTTGGACGTGGACAAAGCAAAAACTGTCATCGTTATTAAGCGAAG
TCTAAGCCCGGGGTTTGCGGGAGTTCCCAACCCGCTCTTTATCCAAGATAACGCCCTTATGTATTTCCAAGACGGTAAAA
AAGCGACTCAGGAAATCGTAACAGCTCTGAAAGATACCTAA

Upstream 100 bases:

>100_bases
GGATTTATCGCGATGGTCGCCGCAACGATCAACGTTGTAGGCGGGTTCTTAGTGACCCATAGAATGTTGGGAATGTTCAA
GAAGAAAGAAAAATAAGAAT

Downstream 100 bases:

>100_bases
AAGGTTCGTTTTTTCAAGAACACACTAAAAAGCTGGACTGTAAAGTTCGGCTTTTTTATTCTGACAAATCTCTCCTTCAC
TAAAATAACTCTTACTATGA

Product: NAD(P)(+) transhydrogenase, beta component

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MEKSIINLVYLLSSILFIVGLKLLSHPKTAVKGNFTGAVGMFFAIAAALIEKGLAYEYILAGFIVGTAIGVYLSVKVEMT
AMPQLVAALNGFGGLASFLVAGAAVMEVLHQGTNLAVLKSYQFTISTAASGIIGAVTLTGSFVAYGKLQGILSEKAVRYP
GDQLVKILFFLGSIALSYFVVIQPEKIEWYWYVVVAGSILGILLVMPIGGADMPVVIALLNSYSGIAASATGFVLGNNVL
IIAGSLVGASGIILTQIMCKAMNRSLPNVLFGGLGAAVDNSKGGEDIYAGKTKSTSAEEVAMLLDMAQRVVIVPGYGMAV
AQAQHAVRDLYNILTDKGIDVEFAIHPVAGRMPGHMNVLLAEADIPYDKMKEMDEINPSFEQVDVVIVNGANDVVNPLAK
TDPSSPIAGMPILDVDKAKTVIVIKRSLSPGFAGVPNPLFIQDNALMYFQDGKKATQEIVTALKDT

Sequences:

>Translated_466_residues
MEKSIINLVYLLSSILFIVGLKLLSHPKTAVKGNFTGAVGMFFAIAAALIEKGLAYEYILAGFIVGTAIGVYLSVKVEMT
AMPQLVAALNGFGGLASFLVAGAAVMEVLHQGTNLAVLKSYQFTISTAASGIIGAVTLTGSFVAYGKLQGILSEKAVRYP
GDQLVKILFFLGSIALSYFVVIQPEKIEWYWYVVVAGSILGILLVMPIGGADMPVVIALLNSYSGIAASATGFVLGNNVL
IIAGSLVGASGIILTQIMCKAMNRSLPNVLFGGLGAAVDNSKGGEDIYAGKTKSTSAEEVAMLLDMAQRVVIVPGYGMAV
AQAQHAVRDLYNILTDKGIDVEFAIHPVAGRMPGHMNVLLAEADIPYDKMKEMDEINPSFEQVDVVIVNGANDVVNPLAK
TDPSSPIAGMPILDVDKAKTVIVIKRSLSPGFAGVPNPLFIQDNALMYFQDGKKATQEIVTALKDT
>Mature_466_residues
MEKSIINLVYLLSSILFIVGLKLLSHPKTAVKGNFTGAVGMFFAIAAALIEKGLAYEYILAGFIVGTAIGVYLSVKVEMT
AMPQLVAALNGFGGLASFLVAGAAVMEVLHQGTNLAVLKSYQFTISTAASGIIGAVTLTGSFVAYGKLQGILSEKAVRYP
GDQLVKILFFLGSIALSYFVVIQPEKIEWYWYVVVAGSILGILLVMPIGGADMPVVIALLNSYSGIAASATGFVLGNNVL
IIAGSLVGASGIILTQIMCKAMNRSLPNVLFGGLGAAVDNSKGGEDIYAGKTKSTSAEEVAMLLDMAQRVVIVPGYGMAV
AQAQHAVRDLYNILTDKGIDVEFAIHPVAGRMPGHMNVLLAEADIPYDKMKEMDEINPSFEQVDVVIVNGANDVVNPLAK
TDPSSPIAGMPILDVDKAKTVIVIKRSLSPGFAGVPNPLFIQDNALMYFQDGKKATQEIVTALKDT

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=475, Percent_Identity=40.8421052631579, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI122939153, Length=475, Percent_Identity=40.8421052631579, Blast_Score=315, Evalue=5e-86,
Organism=Escherichia coli, GI1787886, Length=473, Percent_Identity=46.9344608879493, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17550456, Length=472, Percent_Identity=41.1016949152542, Blast_Score=310, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 49127; Mature: 49127

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSIINLVYLLSSILFIVGLKLLSHPKTAVKGNFTGAVGMFFAIAAALIEKGLAYEYIL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
AGFIVGTAIGVYLSVKVEMTAMPQLVAALNGFGGLASFLVAGAAVMEVLHQGTNLAVLKS
HHHHHHHHHHEEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEE
YQFTISTAASGIIGAVTLTGSFVAYGKLQGILSEKAVRYPGDQLVKILFFLGSIALSYFV
EEEEEEHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEE
VIQPEKIEWYWYVVVAGSILGILLVMPIGGADMPVVIALLNSYSGIAASATGFVLGNNVL
EEECCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEECCCCEEECCCEE
IIAGSLVGASGIILTQIMCKAMNRSLPNVLFGGLGAAVDNSKGGEDIYAGKTKSTSAEEV
EEEECHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCCHHHH
AMLLDMAQRVVIVPGYGMAVAQAQHAVRDLYNILTDKGIDVEFAIHPVAGRMPGHMNVLL
HHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEE
AEADIPYDKMKEMDEINPSFEQVDVVIVNGANDVVNPLAKTDPSSPIAGMPILDVDKAKT
EECCCCHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCCE
VIVIKRSLSPGFAGVPNPLFIQDNALMYFQDGKKATQEIVTALKDT
EEEEEECCCCCCCCCCCCEEEECCEEEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKSIINLVYLLSSILFIVGLKLLSHPKTAVKGNFTGAVGMFFAIAAALIEKGLAYEYIL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
AGFIVGTAIGVYLSVKVEMTAMPQLVAALNGFGGLASFLVAGAAVMEVLHQGTNLAVLKS
HHHHHHHHHHEEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEE
YQFTISTAASGIIGAVTLTGSFVAYGKLQGILSEKAVRYPGDQLVKILFFLGSIALSYFV
EEEEEEHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEE
VIQPEKIEWYWYVVVAGSILGILLVMPIGGADMPVVIALLNSYSGIAASATGFVLGNNVL
EEECCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEECCCCEEECCCEE
IIAGSLVGASGIILTQIMCKAMNRSLPNVLFGGLGAAVDNSKGGEDIYAGKTKSTSAEEV
EEEECHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCCHHHH
AMLLDMAQRVVIVPGYGMAVAQAQHAVRDLYNILTDKGIDVEFAIHPVAGRMPGHMNVLL
HHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEE
AEADIPYDKMKEMDEINPSFEQVDVVIVNGANDVVNPLAKTDPSSPIAGMPILDVDKAKT
EECCCCHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCCE
VIVIKRSLSPGFAGVPNPLFIQDNALMYFQDGKKATQEIVTALKDT
EEEEEECCCCCCCCCCCCEEEECCEEEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]