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The map label for this gene is gyrB [H]

Identifier: 116327080

GI number: 116327080

Start: 302315

End: 304450

Strand: Reverse

Name: gyrB [H]

Synonym: LBL_0253

Alternate gene names: 116327080

Gene position: 304450-302315 (Counterclockwise)

Preceding gene: 116327081

Following gene: 116327079

Centisome position: 8.42

GC content: 42.37

Gene sequence:

>2136_bases
ATGAGCGCCGATAAAACCAAAGTAAAAGAAAAATCATCCCAAGAAAGAAATTTTAAAAAGTTATCCAACGTGGAACACGT
CCGAATGAGAACCGGTATGTGGTTGGGTCAAAATTCCGCTTCCACGTTCGAACAACATTTTTTTCGTAAAAATAACGAAG
GAAAATACGAGATCGTTCACGAAGAGCTCGAAGATGTTCCGGCAAAACTGAAATGCCTGGACGAAGCTTGTATGAACGCA
GTGGACGAATATCGTAAAAATCAAAAGGATAAAACGATTCCTGAAAAGGATAAAATGTCTAAATTAATCGTTCAACTTTC
CTCCGACCGGAAATGTGTGACTATTACCGATAACGGACGAGGAATTCCCGCCGCAAACGCGGAAGGTGTTTATCTCCATT
TGATGTACGGGGAAAATTTCGACGATCATGTAAAACAGGATCACGTTGCGGGTCAAAACGGCGTGGGGATCTCCCTTGTG
AGAATGGTCTCCAACTACTTCAAAGTGAAAACCGTAAACAACGGAAGTTCTTTTAAGAAATTGTTCACCGTTCACGACGA
CGTGAAAAAACAAATTCGTTCCTACAAACTTTCCAAAGAGGATATCGAGCGCGTCTTTCTCTACTTCGACGAACACGGAA
AGTTCACCGACTGCAACCTTCTTACCAAGGATCAAATCGACAAACTCAATTCTCTATTAAAGAAAACAAATATGCAGGAA
TCAATCGAAAAGGCTTCCAAAGAGGATCACGGAACTTCGGTAGAGTTCGAACTCAATCCTAAATACTTCAATAACCTGGA
TATTTCGTTTAACGTGGATCTGATGAAACAGTATCTGCAGGATATCGCGATGACTAATCCGGGTTTGGAAGTCCAGTTCG
TTTTTAAAGGTAAAAAAGAGAAGTATAAATTCAAGAAGGGTCTGGACGAGATTTTTTCCCATTCGGATCTTGTTTATTAC
AAAATGGATTATACCGCACCTGGCTCCGCTTCTCAACTTCATTTGGAAACTTATCTGGTAATCGGGCAAAACAAAAATCT
CACTTGGGTGAATTCCATCTTTGCACCGCAGGGCGGTTCTGCGATAGAATATCTGGAAAACAGAATCTGCGACGAGGTTC
GTAAAAAAAGTCAGATCGTGGCTCTTGAGAAAAAACTCAAAACCAGCTCCACTCGTAACGACGTGAGAAACTGCTTTCAC
ATGTATGTGAATGCAAGACTCCTAAATCCTCGCTTTAAATCTCAGGACAAGTCTTACCTCATCAACGACCTGAATGAGGA
TATCCGCAACGCTGTGGATAAACATCTTGAAAAGTTCATCAAAAAGACGGGGCTTCTGGAAGAGATAAAACTTCAGATGG
AAAAACGAACTCAACTCAAGGCCTTTGAAGACGCTCAAAAAGGTCTTAAAAAAGCGAGCCGCATGAACATTCCAAAACTC
ATGCCTCCGACCGGAAAACCAGGAGATCCAGGAAGGGTATTGTTCGTTGCGGAAGGGGATTCCGCAATCGCAGGCCTTCG
TCCCGCAAGAAACCCGAAGCTGCACGGCTTATTTCCTCTAAGAGGAAAGCCTCTCAACTGTAAAGGTTTAAGCATTGCAA
AGGCAATCGCAAACGAAGAATTAAAAAATATCGTCGCGATCGTCGGCCTACCATTGGATCAGAAAGTAAAATCCGTAGAC
GAACTAAACTACGATAAGGTCAGCATCATCACCGATGCCGATTTCGACGGATATGCGATCCGATCCTTGATGCTTTCCTT
CTTTTACGAATACTGGCCTGAACTTTTCGAGCTTGGTTTTATTCATATTTCCAGCGCTCCTCTTTACGAAGTGGACGTGA
AATTCGGGGATAAGAAGAAAGAAACCGTGTTCTGCATCGACGATAAGGATTACGAAGATCTAATCAAGCGTGTGGAAAAA
AACGGAGGAGAAATTACTCGCAAAAAACGGAATAAAGGACTCGGAGAAACGGGTAAAGAAGCGATGAAGTTTGCAGTCGA
TGAGTGTATGACTAAGATCACGATCGGAAATAAAAAAGACGCTTCTAAAATTCAGAATCTCTGGTTCCACAAGGACTTTG
CGGAACAAAGAAGGGATGCTATCTCCGAATACGCGATGAGTGTGATCGAAGACTAA

Upstream 100 bases:

>100_bases
CGACCCTGGCTCGTTATCAAGGTCTGGTCAAAGAGGATAAAAAACTGACCGACTATATGATCACCGAGTTGAACGAACTT
CAAAACAAATGGGATAAAGT

Downstream 100 bases:

>100_bases
CGTTCGCATTATCTTCGAATTTTATAGAAATTTTCAGAAGAGCAGTTTTCTCCTCAAAAAGAATTCATTATGCTCCTTGT
ATATTAGAGTTATTGGAAAA

Product: DNA topoisomerase (ATP-hydrolyzing), subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MSADKTKVKEKSSQERNFKKLSNVEHVRMRTGMWLGQNSASTFEQHFFRKNNEGKYEIVHEELEDVPAKLKCLDEACMNA
VDEYRKNQKDKTIPEKDKMSKLIVQLSSDRKCVTITDNGRGIPAANAEGVYLHLMYGENFDDHVKQDHVAGQNGVGISLV
RMVSNYFKVKTVNNGSSFKKLFTVHDDVKKQIRSYKLSKEDIERVFLYFDEHGKFTDCNLLTKDQIDKLNSLLKKTNMQE
SIEKASKEDHGTSVEFELNPKYFNNLDISFNVDLMKQYLQDIAMTNPGLEVQFVFKGKKEKYKFKKGLDEIFSHSDLVYY
KMDYTAPGSASQLHLETYLVIGQNKNLTWVNSIFAPQGGSAIEYLENRICDEVRKKSQIVALEKKLKTSSTRNDVRNCFH
MYVNARLLNPRFKSQDKSYLINDLNEDIRNAVDKHLEKFIKKTGLLEEIKLQMEKRTQLKAFEDAQKGLKKASRMNIPKL
MPPTGKPGDPGRVLFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGLSIAKAIANEELKNIVAIVGLPLDQKVKSVD
ELNYDKVSIITDADFDGYAIRSLMLSFFYEYWPELFELGFIHISSAPLYEVDVKFGDKKKETVFCIDDKDYEDLIKRVEK
NGGEITRKKRNKGLGETGKEAMKFAVDECMTKITIGNKKDASKIQNLWFHKDFAEQRRDAISEYAMSVIED

Sequences:

>Translated_711_residues
MSADKTKVKEKSSQERNFKKLSNVEHVRMRTGMWLGQNSASTFEQHFFRKNNEGKYEIVHEELEDVPAKLKCLDEACMNA
VDEYRKNQKDKTIPEKDKMSKLIVQLSSDRKCVTITDNGRGIPAANAEGVYLHLMYGENFDDHVKQDHVAGQNGVGISLV
RMVSNYFKVKTVNNGSSFKKLFTVHDDVKKQIRSYKLSKEDIERVFLYFDEHGKFTDCNLLTKDQIDKLNSLLKKTNMQE
SIEKASKEDHGTSVEFELNPKYFNNLDISFNVDLMKQYLQDIAMTNPGLEVQFVFKGKKEKYKFKKGLDEIFSHSDLVYY
KMDYTAPGSASQLHLETYLVIGQNKNLTWVNSIFAPQGGSAIEYLENRICDEVRKKSQIVALEKKLKTSSTRNDVRNCFH
MYVNARLLNPRFKSQDKSYLINDLNEDIRNAVDKHLEKFIKKTGLLEEIKLQMEKRTQLKAFEDAQKGLKKASRMNIPKL
MPPTGKPGDPGRVLFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGLSIAKAIANEELKNIVAIVGLPLDQKVKSVD
ELNYDKVSIITDADFDGYAIRSLMLSFFYEYWPELFELGFIHISSAPLYEVDVKFGDKKKETVFCIDDKDYEDLIKRVEK
NGGEITRKKRNKGLGETGKEAMKFAVDECMTKITIGNKKDASKIQNLWFHKDFAEQRRDAISEYAMSVIED
>Mature_710_residues
SADKTKVKEKSSQERNFKKLSNVEHVRMRTGMWLGQNSASTFEQHFFRKNNEGKYEIVHEELEDVPAKLKCLDEACMNAV
DEYRKNQKDKTIPEKDKMSKLIVQLSSDRKCVTITDNGRGIPAANAEGVYLHLMYGENFDDHVKQDHVAGQNGVGISLVR
MVSNYFKVKTVNNGSSFKKLFTVHDDVKKQIRSYKLSKEDIERVFLYFDEHGKFTDCNLLTKDQIDKLNSLLKKTNMQES
IEKASKEDHGTSVEFELNPKYFNNLDISFNVDLMKQYLQDIAMTNPGLEVQFVFKGKKEKYKFKKGLDEIFSHSDLVYYK
MDYTAPGSASQLHLETYLVIGQNKNLTWVNSIFAPQGGSAIEYLENRICDEVRKKSQIVALEKKLKTSSTRNDVRNCFHM
YVNARLLNPRFKSQDKSYLINDLNEDIRNAVDKHLEKFIKKTGLLEEIKLQMEKRTQLKAFEDAQKGLKKASRMNIPKLM
PPTGKPGDPGRVLFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGLSIAKAIANEELKNIVAIVGLPLDQKVKSVDE
LNYDKVSIITDADFDGYAIRSLMLSFFYEYWPELFELGFIHISSAPLYEVDVKFGDKKKETVFCIDDKDYEDLIKRVEKN
GGEITRKKRNKGLGETGKEAMKFAVDECMTKITIGNKKDASKIQNLWFHKDFAEQRRDAISEYAMSVIED

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=705, Percent_Identity=26.241134751773, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI19913406, Length=750, Percent_Identity=25.3333333333333, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI48994957, Length=627, Percent_Identity=22.6475279106858, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789408, Length=634, Percent_Identity=21.4511041009464, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17535065, Length=717, Percent_Identity=23.4309623430962, Blast_Score=122, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=621, Percent_Identity=26.0869565217391, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=254, Percent_Identity=29.9212598425197, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=381, Percent_Identity=26.246719160105, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17136538, Length=643, Percent_Identity=25.3499222395023, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 81495; Mature: 81364

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADKTKVKEKSSQERNFKKLSNVEHVRMRTGMWLGQNSASTFEQHFFRKNNEGKYEIVH
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEECCCCHHHHHHHHHHCCCCCCEEEHH
EELEDVPAKLKCLDEACMNAVDEYRKNQKDKTIPEKDKMSKLIVQLSSDRKCVTITDNGR
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCC
GIPAANAEGVYLHLMYGENFDDHVKQDHVAGQNGVGISLVRMVSNYFKVKTVNNGSSFKK
CCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEEECCCCHHHH
LFTVHDDVKKQIRSYKLSKEDIERVFLYFDEHGKFTDCNLLTKDQIDKLNSLLKKTNMQE
HHHHHHHHHHHHHHHCCCHHHHHEEEEEEECCCCEECCCCCCHHHHHHHHHHHHHCCHHH
SIEKASKEDHGTSVEFELNPKYFNNLDISFNVDLMKQYLQDIAMTNPGLEVQFVFKGKKE
HHHHHCCCCCCCEEEEEECCHHHCCCCEEECHHHHHHHHHHHHHCCCCCEEEEEECCCHH
KYKFKKGLDEIFSHSDLVYYKMDYTAPGSASQLHLETYLVIGQNKNLTWVNSIFAPQGGS
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEHEECCCCCH
AIEYLENRICDEVRKKSQIVALEKKLKTSSTRNDVRNCFHMYVNARLLNPRFKSQDKSYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHH
INDLNEDIRNAVDKHLEKFIKKTGLLEEIKLQMEKRTQLKAFEDAQKGLKKASRMNIPKL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MPPTGKPGDPGRVLFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGLSIAKAIANEE
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
LKNIVAIVGLPLDQKVKSVDELNYDKVSIITDADFDGYAIRSLMLSFFYEYWPELFELGF
HHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCE
IHISSAPLYEVDVKFGDKKKETVFCIDDKDYEDLIKRVEKNGGEITRKKRNKGLGETGKE
EEECCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCCCHHHH
AMKFAVDECMTKITIGNKKDASKIQNLWFHKDFAEQRRDAISEYAMSVIED
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SADKTKVKEKSSQERNFKKLSNVEHVRMRTGMWLGQNSASTFEQHFFRKNNEGKYEIVH
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEECCCCHHHHHHHHHHCCCCCCEEEHH
EELEDVPAKLKCLDEACMNAVDEYRKNQKDKTIPEKDKMSKLIVQLSSDRKCVTITDNGR
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCC
GIPAANAEGVYLHLMYGENFDDHVKQDHVAGQNGVGISLVRMVSNYFKVKTVNNGSSFKK
CCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEEECCCCHHHH
LFTVHDDVKKQIRSYKLSKEDIERVFLYFDEHGKFTDCNLLTKDQIDKLNSLLKKTNMQE
HHHHHHHHHHHHHHHCCCHHHHHEEEEEEECCCCEECCCCCCHHHHHHHHHHHHHCCHHH
SIEKASKEDHGTSVEFELNPKYFNNLDISFNVDLMKQYLQDIAMTNPGLEVQFVFKGKKE
HHHHHCCCCCCCEEEEEECCHHHCCCCEEECHHHHHHHHHHHHHCCCCCEEEEEECCCHH
KYKFKKGLDEIFSHSDLVYYKMDYTAPGSASQLHLETYLVIGQNKNLTWVNSIFAPQGGS
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEHEECCCCCH
AIEYLENRICDEVRKKSQIVALEKKLKTSSTRNDVRNCFHMYVNARLLNPRFKSQDKSYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHH
INDLNEDIRNAVDKHLEKFIKKTGLLEEIKLQMEKRTQLKAFEDAQKGLKKASRMNIPKL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MPPTGKPGDPGRVLFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGLSIAKAIANEE
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
LKNIVAIVGLPLDQKVKSVDELNYDKVSIITDADFDGYAIRSLMLSFFYEYWPELFELGF
HHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCE
IHISSAPLYEVDVKFGDKKKETVFCIDDKDYEDLIKRVEKNGGEITRKKRNKGLGETGKE
EEECCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCCCHHHH
AMKFAVDECMTKITIGNKKDASKIQNLWFHKDFAEQRRDAISEYAMSVIED
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]