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The map label for this gene is cysI [H]

Identifier: 116327061

GI number: 116327061

Start: 280183

End: 281865

Strand: Direct

Name: cysI [H]

Synonym: LBL_0234

Alternate gene names: 116327061

Gene position: 280183-281865 (Clockwise)

Preceding gene: 116327060

Following gene: 116327064

Centisome position: 7.75

GC content: 44.92

Gene sequence:

>1683_bases
ATGTCGGAAACGAAAGAACTATCACCAGTCGAAGAAATTAAATTGAACTCAAGGAATCTGAGAGGAAAAATCGCGGAAGG
TTTAGACCGAAACATTGATTCCTACGAGGAAGACGAGAAACAATTACTTAAATTCCACGGACTTTACCAGCAAAAAGATA
GGGACCGCAAAAAAGACGAAAGCGGAAACGATATGGAAGCTCCTACCAGCTTTATGATTCGTGGGAGAATTCCCGGAGGG
AGACTTACTCCCGAGCAATACTTAGTTTGGGATGAATTGGGGGATAAATTTGGAGGCGGAGCGATTCGTTTAACAACGAG
ACAATCGGTTCAGCTTCATACATTAAGGATCTTTCATCTCCGCGACGTAATGAAAGCGATTCACGAAGTAAATCTCTCAA
GTATGGGAGCGTGCGGAGACGTTGTGCGTAACGTGACTCAAGCCGTAAATCCTCTTGGAAAAAAAGAACTTCAGCTTCTC
GACGGGGTTTCTCAGATTTTATCCGATCACTTTAAGTACAAAACAAACGCTTATGCCGAAGTATGGTTAGGCGACAAACA
ACTCAACAAAGACGAGGAAGAGACGATCTACGGAAAAACGTATCTTCCTAGAAAGTTTAAAATCGCCGTTACTCTTGCGG
GAAACAACACCGTGGACATCTACGCAAATGATATGGGGTTTGCGGCGACTCTTTCCGCAAATGGAAAGATTGACGGTTAT
TTCGTGTTTGCCGGAGGAGGTTTCGGAATGACTCATAATAAACCGGAAACCTTCGCACGTGCCGCAAGCCTTCTTGGATG
GATTCCCGAAAGCGCATTGATCCCGGTTGCGGAAGCGATCGTAACAGCTCATAGAGATTTCGGAGATAGAACTAATCGGA
AACACGCTCGTTTGAAATATGTTCTCGCAGAAAAGGGAGTGGATTGGTTTAGATCGGAAGTGGAATCCCGTTCCAATGTA
AAATTGGATACGGATAAATCCCTTCCAAGTTGGAAGACTCCTTCTTATTTGGGTTGGAATGAAAGAGAAGACGGAACCCT
TTCTCTCGGGTTTCATACTCTTGCAGGAAGAATCAAGGATTTTCCCGGAAGACCTTTGAAGTCCGCCCTGAGAGAAATCA
TCGGTATTTACAAATTACACGTTCAGATCACGGCGGATCAGGATTTGATTCTCATCGGAATCCAAAAATCGGATCGTGAA
AAGATCGAAAAAAGATTACAAGAATTGAATGTACCTTGGAAAAGCCCTTCCCCTCTTTATGATCGCGCACTTGCTTGTCC
GGCGCTTCCGACTTGTGCGCTTGCGTTGACGGAATCGGAACGTTCTTTTCCCGAAGTTCTGAACGGAATCCAAAAAGTTT
TGGATAAACTCGGGCTCGGCGATAGAGCTCCTGTGGTTCGTATGACCGGATGTCCGAACGGATGTGCAAGACCTTATTCT
GCGGAAGTGGGAATTGTAGGTCAGCAAGCTGGAGGAAAATACTCATTATTTTTCGGGGCGGATTCCGAAGGAACCAAGGT
CGGCGAATATGTGGCTAAGAAAGTCGCTCTTGCGGATATCCCGGCTCAATTGGAAAAAGTTTTCGTACTCTGGAAAGAAG
AAGGGGACTCCGATGAGAAATTCGGAGATTTCGTAAATAGATTCCCTCTCGAAAGATTCAGAGAAGTATTGGGGTCTATG
TAA

Upstream 100 bases:

>100_bases
AGTTGTTACAAAACCTCAAAGATGGTTATTTTAAGGTTTCGACCGACTCAAAGAGAATATAACGAAACTATAAATCTGAA
AAAGGAAAAAGATCCAAACC

Downstream 100 bases:

>100_bases
GGATTAGAGTATTAGAAAAATTGAATTTCTTCGATTATCGGTTTCATTTTTGCGATTTTACCGGTGGTCGAAGAAACGAG
ATAGATATTTCCCGCGTGTT

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MSETKELSPVEEIKLNSRNLRGKIAEGLDRNIDSYEEDEKQLLKFHGLYQQKDRDRKKDESGNDMEAPTSFMIRGRIPGG
RLTPEQYLVWDELGDKFGGGAIRLTTRQSVQLHTLRIFHLRDVMKAIHEVNLSSMGACGDVVRNVTQAVNPLGKKELQLL
DGVSQILSDHFKYKTNAYAEVWLGDKQLNKDEEETIYGKTYLPRKFKIAVTLAGNNTVDIYANDMGFAATLSANGKIDGY
FVFAGGGFGMTHNKPETFARAASLLGWIPESALIPVAEAIVTAHRDFGDRTNRKHARLKYVLAEKGVDWFRSEVESRSNV
KLDTDKSLPSWKTPSYLGWNEREDGTLSLGFHTLAGRIKDFPGRPLKSALREIIGIYKLHVQITADQDLILIGIQKSDRE
KIEKRLQELNVPWKSPSPLYDRALACPALPTCALALTESERSFPEVLNGIQKVLDKLGLGDRAPVVRMTGCPNGCARPYS
AEVGIVGQQAGGKYSLFFGADSEGTKVGEYVAKKVALADIPAQLEKVFVLWKEEGDSDEKFGDFVNRFPLERFREVLGSM

Sequences:

>Translated_560_residues
MSETKELSPVEEIKLNSRNLRGKIAEGLDRNIDSYEEDEKQLLKFHGLYQQKDRDRKKDESGNDMEAPTSFMIRGRIPGG
RLTPEQYLVWDELGDKFGGGAIRLTTRQSVQLHTLRIFHLRDVMKAIHEVNLSSMGACGDVVRNVTQAVNPLGKKELQLL
DGVSQILSDHFKYKTNAYAEVWLGDKQLNKDEEETIYGKTYLPRKFKIAVTLAGNNTVDIYANDMGFAATLSANGKIDGY
FVFAGGGFGMTHNKPETFARAASLLGWIPESALIPVAEAIVTAHRDFGDRTNRKHARLKYVLAEKGVDWFRSEVESRSNV
KLDTDKSLPSWKTPSYLGWNEREDGTLSLGFHTLAGRIKDFPGRPLKSALREIIGIYKLHVQITADQDLILIGIQKSDRE
KIEKRLQELNVPWKSPSPLYDRALACPALPTCALALTESERSFPEVLNGIQKVLDKLGLGDRAPVVRMTGCPNGCARPYS
AEVGIVGQQAGGKYSLFFGADSEGTKVGEYVAKKVALADIPAQLEKVFVLWKEEGDSDEKFGDFVNRFPLERFREVLGSM
>Mature_559_residues
SETKELSPVEEIKLNSRNLRGKIAEGLDRNIDSYEEDEKQLLKFHGLYQQKDRDRKKDESGNDMEAPTSFMIRGRIPGGR
LTPEQYLVWDELGDKFGGGAIRLTTRQSVQLHTLRIFHLRDVMKAIHEVNLSSMGACGDVVRNVTQAVNPLGKKELQLLD
GVSQILSDHFKYKTNAYAEVWLGDKQLNKDEEETIYGKTYLPRKFKIAVTLAGNNTVDIYANDMGFAATLSANGKIDGYF
VFAGGGFGMTHNKPETFARAASLLGWIPESALIPVAEAIVTAHRDFGDRTNRKHARLKYVLAEKGVDWFRSEVESRSNVK
LDTDKSLPSWKTPSYLGWNEREDGTLSLGFHTLAGRIKDFPGRPLKSALREIIGIYKLHVQITADQDLILIGIQKSDREK
IEKRLQELNVPWKSPSPLYDRALACPALPTCALALTESERSFPEVLNGIQKVLDKLGLGDRAPVVRMTGCPNGCARPYSA
EVGIVGQQAGGKYSLFFGADSEGTKVGEYVAKKVALADIPAQLEKVFVLWKEEGDSDEKFGDFVNRFPLERFREVLGSM

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=548, Percent_Identity=37.4087591240876, Blast_Score=377, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6322597, Length=572, Percent_Identity=34.2657342657343, Blast_Score=335, Evalue=9e-93,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 62533; Mature: 62402

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETKELSPVEEIKLNSRNLRGKIAEGLDRNIDSYEEDEKQLLKFHGLYQQKDRDRKKDE
CCCCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
SGNDMEAPTSFMIRGRIPGGRLTPEQYLVWDELGDKFGGGAIRLTTRQSVQLHTLRIFHL
CCCCCCCCCEEEEEECCCCCCCCHHHEEEHHHHCCCCCCCEEEEEECCCEEEEHHHHHHH
RDVMKAIHEVNLSSMGACGDVVRNVTQAVNPLGKKELQLLDGVSQILSDHFKYKTNAYAE
HHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
VWLGDKQLNKDEEETIYGKTYLPRKFKIAVTLAGNNTVDIYANDMGFAATLSANGKIDGY
EEECCHHCCCCHHHEEECCEECCCEEEEEEEEECCCEEEEEECCCCEEEEEECCCCEEEE
FVFAGGGFGMTHNKPETFARAASLLGWIPESALIPVAEAIVTAHRDFGDRTNRKHARLKY
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VLAEKGVDWFRSEVESRSNVKLDTDKSLPSWKTPSYLGWNEREDGTLSLGFHTLAGRIKD
HHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHC
FPGRPLKSALREIIGIYKLHVQITADQDLILIGIQKSDREKIEKRLQELNVPWKSPSPLY
CCCCHHHHHHHHHHCEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHH
DRALACPALPTCALALTESERSFPEVLNGIQKVLDKLGLGDRAPVVRMTGCPNGCARPYS
HHHHCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
AEVGIVGQQAGGKYSLFFGADSEGTKVGEYVAKKVALADIPAQLEKVFVLWKEEGDSDEK
CCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHEEEEEECCCCCHHH
FGDFVNRFPLERFREVLGSM
HHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
SETKELSPVEEIKLNSRNLRGKIAEGLDRNIDSYEEDEKQLLKFHGLYQQKDRDRKKDE
CCCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
SGNDMEAPTSFMIRGRIPGGRLTPEQYLVWDELGDKFGGGAIRLTTRQSVQLHTLRIFHL
CCCCCCCCCEEEEEECCCCCCCCHHHEEEHHHHCCCCCCCEEEEEECCCEEEEHHHHHHH
RDVMKAIHEVNLSSMGACGDVVRNVTQAVNPLGKKELQLLDGVSQILSDHFKYKTNAYAE
HHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
VWLGDKQLNKDEEETIYGKTYLPRKFKIAVTLAGNNTVDIYANDMGFAATLSANGKIDGY
EEECCHHCCCCHHHEEECCEECCCEEEEEEEEECCCEEEEEECCCCEEEEEECCCCEEEE
FVFAGGGFGMTHNKPETFARAASLLGWIPESALIPVAEAIVTAHRDFGDRTNRKHARLKY
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VLAEKGVDWFRSEVESRSNVKLDTDKSLPSWKTPSYLGWNEREDGTLSLGFHTLAGRIKD
HHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHC
FPGRPLKSALREIIGIYKLHVQITADQDLILIGIQKSDREKIEKRLQELNVPWKSPSPLY
CCCCHHHHHHHHHHCEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHH
DRALACPALPTCALALTESERSFPEVLNGIQKVLDKLGLGDRAPVVRMTGCPNGCARPYS
HHHHCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
AEVGIVGQQAGGKYSLFFGADSEGTKVGEYVAKKVALADIPAQLEKVFVLWKEEGDSDEK
CCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHEEEEEECCCCCHHH
FGDFVNRFPLERFREVLGSM
HHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA