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The map label for this gene is cysH-2 [H]

Identifier: 116327057

GI number: 116327057

Start: 276535

End: 277452

Strand: Direct

Name: cysH-2 [H]

Synonym: LBL_0230

Alternate gene names: 116327057

Gene position: 276535-277452 (Clockwise)

Preceding gene: 116327056

Following gene: 116327058

Centisome position: 7.65

GC content: 48.15

Gene sequence:

>918_bases
ATGAATTCAACTATGAATAGATCCAGACTCACACATCTCGAACAGCTGGAAGCGGAATCGATTTATATTCTTCGGGAAAC
TGCTTCTCAGTTTGAAAGACCCGCTCTTCTTTTTTCGGGCGGAAAGGATTCGATCACGCTTGTGCATCTCGCGTTGAAGG
CGTTTCGCCCCGGCAAGTTTCCGTTTCCACTCGTTCACATCGATACCGGTCATAACTTTCAAGAGGCTCTCGACTTTAGA
GACAAACTCGCTTCCAAAATCGGCGAGAAGCTGATCGTCCGTTACGTTCAAGATTCCATCGATCAAGGCAAAGCGGTCGA
AGAAAAAGGAAAGTTCCCGAGTAGAAATGGAATTCAAACCGTGACACTTTTGGATACGATCTCGGAATTCAAGTTCGACG
CTTGTATCGGTGGTGCCCGCAGGGACGAAGAAAAAGCCAGAGCGAAAGAAAGGGTCTTTTCGGTTCGAGACGAATTCGGT
CAATGGGATCCGAAACTGCAAAGACCCGAACTTTGGAATATCTACAACGGCAAGATCAGTCCGGGCGAGAATGTTCGCGT
TTTCCCGATTTCCAACTGGACCGAGTTGGACGTTTGGGAATACATCCGAAAGGAAAACATTGCCCTTCCTTCCTTATATT
TTTCTCATAAACGACAAGTGATCTATCGCGAGAATCTTCTCTTCCCTGTGTCGAAGTTCATTACGATCGACAGAAACGAT
CGCGTGGAAGACAAGATCGTTCGTTTCCGCACGGTGGGGGATATGACTTGCACCGCGGCCGTGGATTCGCAAGCGGATAA
CATCGACGACATCATCCTCGAAATCCAGACGACTCGGACTACCGAAAGAGGTTCTAGGCTCGACGACAAACGTTCCGAAG
CGGCTATGGAAGACAGAAAAAGGGGAGGATACTTCTGA

Upstream 100 bases:

>100_bases
GCGCCGGAAGGTGGTGGTGGGAAGCGTCCAATCAAGAATGTGGACTTCACGTCGTAGATGGAAAGTTGGTCCGTCAAAAA
TCCGGTCCCAAAAGAGCAAT

Downstream 100 bases:

>100_bases
TGGATTTATTACGTTTTATCACTGCGGGAAGCGTGGATGACGGAAAATCCACATTGATCGGAAGACTTCTCTATGATAGC
AAATCGATTTTCGAAGACCA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNSTMNRSRLTHLEQLEAESIYILRETASQFERPALLFSGGKDSITLVHLALKAFRPGKFPFPLVHIDTGHNFQEALDFR
DKLASKIGEKLIVRYVQDSIDQGKAVEEKGKFPSRNGIQTVTLLDTISEFKFDACIGGARRDEEKARAKERVFSVRDEFG
QWDPKLQRPELWNIYNGKISPGENVRVFPISNWTELDVWEYIRKENIALPSLYFSHKRQVIYRENLLFPVSKFITIDRND
RVEDKIVRFRTVGDMTCTAAVDSQADNIDDIILEIQTTRTTERGSRLDDKRSEAAMEDRKRGGYF

Sequences:

>Translated_305_residues
MNSTMNRSRLTHLEQLEAESIYILRETASQFERPALLFSGGKDSITLVHLALKAFRPGKFPFPLVHIDTGHNFQEALDFR
DKLASKIGEKLIVRYVQDSIDQGKAVEEKGKFPSRNGIQTVTLLDTISEFKFDACIGGARRDEEKARAKERVFSVRDEFG
QWDPKLQRPELWNIYNGKISPGENVRVFPISNWTELDVWEYIRKENIALPSLYFSHKRQVIYRENLLFPVSKFITIDRND
RVEDKIVRFRTVGDMTCTAAVDSQADNIDDIILEIQTTRTTERGSRLDDKRSEAAMEDRKRGGYF
>Mature_305_residues
MNSTMNRSRLTHLEQLEAESIYILRETASQFERPALLFSGGKDSITLVHLALKAFRPGKFPFPLVHIDTGHNFQEALDFR
DKLASKIGEKLIVRYVQDSIDQGKAVEEKGKFPSRNGIQTVTLLDTISEFKFDACIGGARRDEEKARAKERVFSVRDEFG
QWDPKLQRPELWNIYNGKISPGENVRVFPISNWTELDVWEYIRKENIALPSLYFSHKRQVIYRENLLFPVSKFITIDRND
RVEDKIVRFRTVGDMTCTAAVDSQADNIDDIILEIQTTRTTERGSRLDDKRSEAAMEDRKRGGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=46.3576158940397, Blast_Score=284, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35256; Mature: 35256

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTMNRSRLTHLEQLEAESIYILRETASQFERPALLFSGGKDSITLVHLALKAFRPGKF
CCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCC
PFPLVHIDTGHNFQEALDFRDKLASKIGEKLIVRYVQDSIDQGKAVEEKGKFPSRNGIQT
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEE
VTLLDTISEFKFDACIGGARRDEEKARAKERVFSVRDEFGQWDPKLQRPELWNIYNGKIS
EHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCC
PGENVRVFPISNWTELDVWEYIRKENIALPSLYFSHKRQVIYRENLLFPVSKFITIDRND
CCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHEEECCCCCEECHHEEEECCCC
RVEDKIVRFRTVGDMTCTAAVDSQADNIDDIILEIQTTRTTERGSRLDDKRSEAAMEDRK
CHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RGGYF
CCCCC
>Mature Secondary Structure
MNSTMNRSRLTHLEQLEAESIYILRETASQFERPALLFSGGKDSITLVHLALKAFRPGKF
CCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCC
PFPLVHIDTGHNFQEALDFRDKLASKIGEKLIVRYVQDSIDQGKAVEEKGKFPSRNGIQT
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEE
VTLLDTISEFKFDACIGGARRDEEKARAKERVFSVRDEFGQWDPKLQRPELWNIYNGKIS
EHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCC
PGENVRVFPISNWTELDVWEYIRKENIALPSLYFSHKRQVIYRENLLFPVSKFITIDRND
CCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHEEECCCCCEECHHEEEECCCC
RVEDKIVRFRTVGDMTCTAAVDSQADNIDDIILEIQTTRTTERGSRLDDKRSEAAMEDRK
CHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RGGYF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA