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The map label for this gene is cysH-1 [H]

Identifier: 116327056

GI number: 116327056

Start: 275801

End: 276538

Strand: Direct

Name: cysH-1 [H]

Synonym: LBL_0229

Alternate gene names: 116327056

Gene position: 275801-276538 (Clockwise)

Preceding gene: 116327053

Following gene: 116327057

Centisome position: 7.63

GC content: 44.99

Gene sequence:

>738_bases
ATGAGTCCGCAAGAGTTGGAACAAAAACTTGCTCCTTTGAGCCTGGAAGATTCCTTAGAATGGATCAATAACGAGTACGG
AGAGACTGCGGCTTTTTCCACGAGCTTAGGTCTTGAGGACCAAGTTATCACTCACGTCATATTCTCCCGAAATCTGAAAA
TCAGAATTTTTACTTTGGACACGGGCCGCCTTTTCAACGAAACGTACGACCTTCACAAACTTACGAACGCGAGTTATAAC
AAAAGGATCGAAACGTATTTTCCAGACACGGCCGCGGTTCAAAATCTAATCAATACGAAAGGTCCCGACAGTTTTTACGA
TTCTGTGGATAACAGAAAAGAATGCTGTTACATCCGGAAAGTAGAGCCGTTAAACCGTGCACTTGTCGGAACAAAACTTT
GGATCACGGGAATTCGGTCGGAACAATCCGATTCCAGACATTCTTTGGCAAAAGTGGAATTCGATTCTTCGCGTAATATT
TTAAAGTATCATCCGCTTCTTGATTGGTCTTTGGAGCGCATTCAAGACTTTATCGATACATATCGGGTTCCGACTAACGT
ATTACACAAGAAAGGTTTTCCTTCGATCGGATGCGCTCCTTGCACGAGAGCCGTTCAGCCCGGAGAAGACATTCGCGCCG
GAAGGTGGTGGTGGGAAGCGTCCAATCAAGAATGTGGACTTCACGTCGTAGATGGAAAGTTGGTCCGTCAAAAATCCGGT
CCCAAAAGAGCAATATGA

Upstream 100 bases:

>100_bases
AATAGTCCCATTTTTTCTTAAATCTGGGCTATTTCTTGGATCAACCATAAATCAAGCTGGATGCGTAAACCTCCTATCCA
AGACTTAGGTTATCACGAAC

Downstream 100 bases:

>100_bases
ATTCAACTATGAATAGATCCAGACTCACACATCTCGAACAGCTGGAAGCGGAATCGATTTATATTCTTCGGGAAACTGCT
TCTCAGTTTGAAAGACCCGC

Product: phosphoadenylyl-sulfate reductase (thioredoxin)

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSPQELEQKLAPLSLEDSLEWINNEYGETAAFSTSLGLEDQVITHVIFSRNLKIRIFTLDTGRLFNETYDLHKLTNASYN
KRIETYFPDTAAVQNLINTKGPDSFYDSVDNRKECCYIRKVEPLNRALVGTKLWITGIRSEQSDSRHSLAKVEFDSSRNI
LKYHPLLDWSLERIQDFIDTYRVPTNVLHKKGFPSIGCAPCTRAVQPGEDIRAGRWWWEASNQECGLHVVDGKLVRQKSG
PKRAI

Sequences:

>Translated_245_residues
MSPQELEQKLAPLSLEDSLEWINNEYGETAAFSTSLGLEDQVITHVIFSRNLKIRIFTLDTGRLFNETYDLHKLTNASYN
KRIETYFPDTAAVQNLINTKGPDSFYDSVDNRKECCYIRKVEPLNRALVGTKLWITGIRSEQSDSRHSLAKVEFDSSRNI
LKYHPLLDWSLERIQDFIDTYRVPTNVLHKKGFPSIGCAPCTRAVQPGEDIRAGRWWWEASNQECGLHVVDGKLVRQKSG
PKRAI
>Mature_244_residues
SPQELEQKLAPLSLEDSLEWINNEYGETAAFSTSLGLEDQVITHVIFSRNLKIRIFTLDTGRLFNETYDLHKLTNASYNK
RIETYFPDTAAVQNLINTKGPDSFYDSVDNRKECCYIRKVEPLNRALVGTKLWITGIRSEQSDSRHSLAKVEFDSSRNIL
KYHPLLDWSLERIQDFIDTYRVPTNVLHKKGFPSIGCAPCTRAVQPGEDIRAGRWWWEASNQECGLHVVDGKLVRQKSGP
KRAI

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=232, Percent_Identity=28.0172413793103, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325425, Length=205, Percent_Identity=32.1951219512195, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 28091; Mature: 27960

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPQELEQKLAPLSLEDSLEWINNEYGETAAFSTSLGLEDQVITHVIFSRNLKIRIFTLD
CCHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCCEEEEEEEC
TGRLFNETYDLHKLTNASYNKRIETYFPDTAAVQNLINTKGPDSFYDSVDNRKECCYIRK
CCHHHHHHHHHHHHCCCHHHCEEEEECCCHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEC
VEPLNRALVGTKLWITGIRSEQSDSRHSLAKVEFDSSRNILKYHPLLDWSLERIQDFIDT
CCHHHHHHHHHEEEEEECCCCCCCCHHCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHH
YRVPTNVLHKKGFPSIGCAPCTRAVQPGEDIRAGRWWWEASNQECGLHVVDGKLVRQKSG
HCCCHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCCCCEEEECCCHHHCCCC
PKRAI
CCCCC
>Mature Secondary Structure 
SPQELEQKLAPLSLEDSLEWINNEYGETAAFSTSLGLEDQVITHVIFSRNLKIRIFTLD
CHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCCEEEEEEEC
TGRLFNETYDLHKLTNASYNKRIETYFPDTAAVQNLINTKGPDSFYDSVDNRKECCYIRK
CCHHHHHHHHHHHHCCCHHHCEEEEECCCHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEC
VEPLNRALVGTKLWITGIRSEQSDSRHSLAKVEFDSSRNILKYHPLLDWSLERIQDFIDT
CCHHHHHHHHHEEEEEECCCCCCCCHHCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHH
YRVPTNVLHKKGFPSIGCAPCTRAVQPGEDIRAGRWWWEASNQECGLHVVDGKLVRQKSG
HCCCHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCCCCEEEECCCHHHCCCC
PKRAI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10464198; 11481430 [H]