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The map label for this gene is truD [H]

Identifier: 116327017

GI number: 116327017

Start: 219451

End: 220659

Strand: Direct

Name: truD [H]

Synonym: LBL_0188

Alternate gene names: 116327017

Gene position: 219451-220659 (Clockwise)

Preceding gene: 116327014

Following gene: 116327022

Centisome position: 6.07

GC content: 38.63

Gene sequence:

>1209_bases
TTGAGTGGGTTTTTAGTTTATGATTTGAAACAGAATCCGGAAGATTTTCGGGTTGAAGAAATTCTTCGTCCCGGTTTTAT
ACAAAAATCCGGGAAATGGACGATTTTCCGACTTCATAAATCCGGTTGGAATACGTTGGACGCTCTCTTGAAAATTTCCA
AGGAATCTAAGGTCTCTATCTCCGAGATCGGATATGCAGGCAAGAAGGATCGACACGCTAACACTTCTCAATATTTAAGT
TGTCAAAAGCCATTGAAAATTCCCAAGGAATTTGCGAACGTTTTGCAATTGGAAAGGATCGGCTTTAGCGAAAAATCCTT
GAGTCCGGAAGCGAATGCCGGCAATCGTTTTGTTCTCATTCTTAGAAATTTACTCGAAAAAGAAATTGAATCGGTTCGGA
ACAATTTTGAAAAGATCGGTAAAAATGGTTTCATCAATTATTATGATTCGCAAAGGTTTAGCCGATTTCATCCGGAGTTT
CGACTCCCTATTTTTCCGTATTTGAAAGGGGACGCTGAAACTTGTCTGAAATTGATTTTGACTGATCCCTATGCGGGGGA
GAAAAAACAAACTCGGGATCGAAAGAAAAAAATTCAGGTCGCTTGGGGAAACTGGTCTCAATGTAAAAAGTGGTCTAACA
ACAAACTGGAAAATAAAATCTTTTTCAATTTAAGTCGGGAAAAGAACCCGACCCAAAAGACGTATTCTACTTTGATTCTT
CAGTTTCCTGAAGAAGAGCTGTTGATGTTGATCTCATCGATGCAATCCTTAATCTGGAACGAATTTGTATCCGAGCTTTT
GGTCTCGGAAGGATGTTCCGGTGTTCGGATCAAAACTAAGACGGGATTTTTATTTTTTCCTGAGGAATCATCCATGAAAT
CCTTGTCGTCTTTTCGAAATCTTCCGGTTCCAGGGGAATTGGGAATTTATAAATTAGAATATTCTAAAAAAGAAGTAGAT
GTTTTAAGGAGAATTCTTGATTTGAACGGGTTAAATGAAGCGGTATTCGACCATTCTCCTTTTCCGGGTATTAAAATGAA
TTCCTTTGAACGAAAAATGAATGTGATTCCGGAAAACTTTCAAATGACGGATTTCGAAGATGACGATTTGCATCCAGGAA
AAAGGAAAGTGAAAATATCCTTTCAACTTCCTTCCGGTGCTTATGCGACGGTGCTGGTCAAACGATTGATGCTCAGAGCG
AATATATAA

Upstream 100 bases:

>100_bases
TTAAACTACTATTTTAAAATGTGTCTTCCGATCAAGGAATTTTTTAATTCAAGATTGTTCTTGTGAGAAGTTTTCATATT
TTGGAACTGTGACAAGTCCA

Downstream 100 bases:

>100_bases
GACGTAATCAAAAATAACTTACAGCGCATCCAAAACCTGCGGAAGGATTTGACATAAGATACATAGAAGAGAGCAGCGCT
TTAGTTTAAAAAATTCCGTT

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSGFLVYDLKQNPEDFRVEEILRPGFIQKSGKWTIFRLHKSGWNTLDALLKISKESKVSISEIGYAGKKDRHANTSQYLS
CQKPLKIPKEFANVLQLERIGFSEKSLSPEANAGNRFVLILRNLLEKEIESVRNNFEKIGKNGFINYYDSQRFSRFHPEF
RLPIFPYLKGDAETCLKLILTDPYAGEKKQTRDRKKKIQVAWGNWSQCKKWSNNKLENKIFFNLSREKNPTQKTYSTLIL
QFPEEELLMLISSMQSLIWNEFVSELLVSEGCSGVRIKTKTGFLFFPEESSMKSLSSFRNLPVPGELGIYKLEYSKKEVD
VLRRILDLNGLNEAVFDHSPFPGIKMNSFERKMNVIPENFQMTDFEDDDLHPGKRKVKISFQLPSGAYATVLVKRLMLRA
NI

Sequences:

>Translated_402_residues
MSGFLVYDLKQNPEDFRVEEILRPGFIQKSGKWTIFRLHKSGWNTLDALLKISKESKVSISEIGYAGKKDRHANTSQYLS
CQKPLKIPKEFANVLQLERIGFSEKSLSPEANAGNRFVLILRNLLEKEIESVRNNFEKIGKNGFINYYDSQRFSRFHPEF
RLPIFPYLKGDAETCLKLILTDPYAGEKKQTRDRKKKIQVAWGNWSQCKKWSNNKLENKIFFNLSREKNPTQKTYSTLIL
QFPEEELLMLISSMQSLIWNEFVSELLVSEGCSGVRIKTKTGFLFFPEESSMKSLSSFRNLPVPGELGIYKLEYSKKEVD
VLRRILDLNGLNEAVFDHSPFPGIKMNSFERKMNVIPENFQMTDFEDDDLHPGKRKVKISFQLPSGAYATVLVKRLMLRA
NI
>Mature_401_residues
SGFLVYDLKQNPEDFRVEEILRPGFIQKSGKWTIFRLHKSGWNTLDALLKISKESKVSISEIGYAGKKDRHANTSQYLSC
QKPLKIPKEFANVLQLERIGFSEKSLSPEANAGNRFVLILRNLLEKEIESVRNNFEKIGKNGFINYYDSQRFSRFHPEFR
LPIFPYLKGDAETCLKLILTDPYAGEKKQTRDRKKKIQVAWGNWSQCKKWSNNKLENKIFFNLSREKNPTQKTYSTLILQ
FPEEELLMLISSMQSLIWNEFVSELLVSEGCSGVRIKTKTGFLFFPEESSMKSLSSFRNLPVPGELGIYKLEYSKKEVDV
LRRILDLNGLNEAVFDHSPFPGIKMNSFERKMNVIPENFQMTDFEDDDLHPGKRKVKISFQLPSGAYATVLVKRLMLRAN
I

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI148762985, Length=415, Percent_Identity=26.2650602409639, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI148762961, Length=415, Percent_Identity=26.2650602409639, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI148762948, Length=415, Percent_Identity=26.2650602409639, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789102, Length=154, Percent_Identity=34.4155844155844, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324817, Length=256, Percent_Identity=29.6875, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21355799, Length=249, Percent_Identity=24.8995983935743, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 46582; Mature: 46451

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50984 TRUD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFLVYDLKQNPEDFRVEEILRPGFIQKSGKWTIFRLHKSGWNTLDALLKISKESKVSI
CCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCH
SEIGYAGKKDRHANTSQYLSCQKPLKIPKEFANVLQLERIGFSEKSLSPEANAGNRFVLI
HHCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHH
LRNLLEKEIESVRNNFEKIGKNGFINYYDSQRFSRFHPEFRLPIFPYLKGDAETCLKLIL
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
TDPYAGEKKQTRDRKKKIQVAWGNWSQCKKWSNNKLENKIFFNLSREKNPTQKTYSTLIL
CCCCCCCHHHHHHHHHEEEEEECCHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHE
QFPEEELLMLISSMQSLIWNEFVSELLVSEGCSGVRIKTKTGFLFFPEESSMKSLSSFRN
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCHHHHHHHHHCC
LPVPGELGIYKLEYSKKEVDVLRRILDLNGLNEAVFDHSPFPGIKMNSFERKMNVIPENF
CCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHCCCCCC
QMTDFEDDDLHPGKRKVKISFQLPSGAYATVLVKRLMLRANI
EECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGFLVYDLKQNPEDFRVEEILRPGFIQKSGKWTIFRLHKSGWNTLDALLKISKESKVSI
CCEEEEECCCCCCCCCHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCH
SEIGYAGKKDRHANTSQYLSCQKPLKIPKEFANVLQLERIGFSEKSLSPEANAGNRFVLI
HHCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHH
LRNLLEKEIESVRNNFEKIGKNGFINYYDSQRFSRFHPEFRLPIFPYLKGDAETCLKLIL
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
TDPYAGEKKQTRDRKKKIQVAWGNWSQCKKWSNNKLENKIFFNLSREKNPTQKTYSTLIL
CCCCCCCHHHHHHHHHEEEEEECCHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHE
QFPEEELLMLISSMQSLIWNEFVSELLVSEGCSGVRIKTKTGFLFFPEESSMKSLSSFRN
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCHHHHHHHHHCC
LPVPGELGIYKLEYSKKEVDVLRRILDLNGLNEAVFDHSPFPGIKMNSFERKMNVIPENF
CCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHCCCCCC
QMTDFEDDDLHPGKRKVKISFQLPSGAYATVLVKRLMLRANI
EECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA