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The map label for this gene is hyfB-2 [H]

Identifier: 116326951

GI number: 116326951

Start: 134308

End: 135516

Strand: Reverse

Name: hyfB-2 [H]

Synonym: LBL_0117

Alternate gene names: 116326951

Gene position: 135516-134308 (Counterclockwise)

Preceding gene: 116326952

Following gene: 116326950

Centisome position: 3.75

GC content: 42.6

Gene sequence:

>1209_bases
ATGAATCTTCTCGTTTTGAACGGGATCGGAGTGATGGTTTTATTTCTGATTCTCCTTGCCTATATTTTAGCGCCTACCAG
AGGTCAAAAACAAATTACAATGTGGTCCCTTTTGATGATTCTCTGTGTCGGATTAACGTTTGCGGCATGGAATTTGGAAG
GGTTCGCTCTTCAATGGGTATTGATCGAAGCCACAACTTTTACGGGAGCGTTGCTCGTATCTTCGAGTAGAACTTCCAAA
TCCTTTCCGATCGCTTGGAAATTTCTTTTAATCAACTCTTTCGGATTGGGAATCGCATTTTTAGGAATCATTATAGTCGT
CGCCACGTTACACGGCATCGATTATCCGGTCGAAGTTTTGGCGGGCAAGTTATCCGAACACCCGGAAAATCTCTGGATTG
AAATCGGGCTTTGGCTGGCGATTTTCGGTTACTCCGCAAAACTCGGACTCTTTCCTAATCACGTTTGGGTCGTGGATACC
TACGGCGAAAGTCCGAGTCAAATCTCCGCATTGATCGCGGCGTTTGTGCCGGTTGCGGTAAGTTACGCCTTACGCCCGTT
CATTCACATGGATCATCAATTGTATCCGACCACTTTCAGCGCGGCGGATGGATTGTTGATTCTAGGAATTATAACAATGT
TACAGAGTATCGTCGCGCTCTATCAGAGGAACGATTTGAGAATGATGACGGCGAAGGTAGCTTTGTTTCACAGTGGTGCG
ATCGGAATTTTTCTTTGGATGGATCTTACAGATTCAGTGTTTAATTTTGTGATGGCCGGAAATATCGTAATGAAATCTGT
CCTATTTTTAACGATGGGGATTGTGAGAATGGATGCCGGGAAAAGGGAATTGAGCAAAATTTTTCTTTCTGAAAAAATCA
ATAAAAAAGCGTTGTCGCTTTATACAGCTTCTTTGTTCGTCGCCTTTATACTTCCCGGTTCTCCAATTTTCGTAAATGAT
CTTTTACTTTTAAAAGTAGGCTGGGCCCAAGGTCAATGGTTTGTGATTACGGTGCCGTTTTTAGGATTGATCTTTTTCGG
AGTACTACTCTACAAAACGGTTCCTCTTTTTAATTTGGAAGATCGTCCGTTTGTAAAGGAATTTGCCGGAACATTACAGA
TCCGAATGACCAATTCGCTTTTGCTTTTTATCATGGTACTGGGTCTCGGTATTTGGGGTTTCTATCATCTACTGCAAGGA
GAATTTTGA

Upstream 100 bases:

>100_bases
TCGGTTCTTTCTTAGATGCGGTTTTTATCATCGGCGCGGCCGCGACTCTTAGAATTAGTATGGAAAGTTCTTCGGAGAAC
GAAAAAGAAGAGGCACCGAT

Downstream 100 bases:

>100_bases
ATGCGTAGCGTGACTGGTTTATACCCGAGAAAAGGTAAAAAGGGTTACAACCGCTTTTGGCTTACAAGCGACGGAGTAGA
ACATGAAATCGTAGAATATT

Product: formate hydrogenase, subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MNLLVLNGIGVMVLFLILLAYILAPTRGQKQITMWSLLMILCVGLTFAAWNLEGFALQWVLIEATTFTGALLVSSSRTSK
SFPIAWKFLLINSFGLGIAFLGIIIVVATLHGIDYPVEVLAGKLSEHPENLWIEIGLWLAIFGYSAKLGLFPNHVWVVDT
YGESPSQISALIAAFVPVAVSYALRPFIHMDHQLYPTTFSAADGLLILGIITMLQSIVALYQRNDLRMMTAKVALFHSGA
IGIFLWMDLTDSVFNFVMAGNIVMKSVLFLTMGIVRMDAGKRELSKIFLSEKINKKALSLYTASLFVAFILPGSPIFVND
LLLLKVGWAQGQWFVITVPFLGLIFFGVLLYKTVPLFNLEDRPFVKEFAGTLQIRMTNSLLLFIMVLGLGIWGFYHLLQG
EF

Sequences:

>Translated_402_residues
MNLLVLNGIGVMVLFLILLAYILAPTRGQKQITMWSLLMILCVGLTFAAWNLEGFALQWVLIEATTFTGALLVSSSRTSK
SFPIAWKFLLINSFGLGIAFLGIIIVVATLHGIDYPVEVLAGKLSEHPENLWIEIGLWLAIFGYSAKLGLFPNHVWVVDT
YGESPSQISALIAAFVPVAVSYALRPFIHMDHQLYPTTFSAADGLLILGIITMLQSIVALYQRNDLRMMTAKVALFHSGA
IGIFLWMDLTDSVFNFVMAGNIVMKSVLFLTMGIVRMDAGKRELSKIFLSEKINKKALSLYTASLFVAFILPGSPIFVND
LLLLKVGWAQGQWFVITVPFLGLIFFGVLLYKTVPLFNLEDRPFVKEFAGTLQIRMTNSLLLFIMVLGLGIWGFYHLLQG
EF
>Mature_402_residues
MNLLVLNGIGVMVLFLILLAYILAPTRGQKQITMWSLLMILCVGLTFAAWNLEGFALQWVLIEATTFTGALLVSSSRTSK
SFPIAWKFLLINSFGLGIAFLGIIIVVATLHGIDYPVEVLAGKLSEHPENLWIEIGLWLAIFGYSAKLGLFPNHVWVVDT
YGESPSQISALIAAFVPVAVSYALRPFIHMDHQLYPTTFSAADGLLILGIITMLQSIVALYQRNDLRMMTAKVALFHSGA
IGIFLWMDLTDSVFNFVMAGNIVMKSVLFLTMGIVRMDAGKRELSKIFLSEKINKKALSLYTASLFVAFILPGSPIFVND
LLLLKVGWAQGQWFVITVPFLGLIFFGVLLYKTVPLFNLEDRPFVKEFAGTLQIRMTNSLLLFIMVLGLGIWGFYHLLQG
EF

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788831, Length=290, Percent_Identity=24.8275862068966, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 44689; Mature: 44689

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLVLNGIGVMVLFLILLAYILAPTRGQKQITMWSLLMILCVGLTFAAWNLEGFALQWV
CCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
LIEATTFTGALLVSSSRTSKSFPIAWKFLLINSFGLGIAFLGIIIVVATLHGIDYPVEVL
EEEEEHHCEEEEEECCCCCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHCCCCCHHHH
AGKLSEHPENLWIEIGLWLAIFGYSAKLGLFPNHVWVVDTYGESPSQISALIAAFVPVAV
HHHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
SYALRPFIHMDHQLYPTTFSAADGLLILGIITMLQSIVALYQRNDLRMMTAKVALFHSGA
HHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
IGIFLWMDLTDSVFNFVMAGNIVMKSVLFLTMGIVRMDAGKRELSKIFLSEKINKKALSL
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHH
YTASLFVAFILPGSPIFVNDLLLLKVGWAQGQWFVITVPFLGLIFFGVLLYKTVPLFNLE
HHHHHHHHHHCCCCCEECCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCC
DRPFVKEFAGTLQIRMTNSLLLFIMVLGLGIWGFYHLLQGEF
CCCHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLLVLNGIGVMVLFLILLAYILAPTRGQKQITMWSLLMILCVGLTFAAWNLEGFALQWV
CCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
LIEATTFTGALLVSSSRTSKSFPIAWKFLLINSFGLGIAFLGIIIVVATLHGIDYPVEVL
EEEEEHHCEEEEEECCCCCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHCCCCCHHHH
AGKLSEHPENLWIEIGLWLAIFGYSAKLGLFPNHVWVVDTYGESPSQISALIAAFVPVAV
HHHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
SYALRPFIHMDHQLYPTTFSAADGLLILGIITMLQSIVALYQRNDLRMMTAKVALFHSGA
HHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
IGIFLWMDLTDSVFNFVMAGNIVMKSVLFLTMGIVRMDAGKRELSKIFLSEKINKKALSL
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHH
YTASLFVAFILPGSPIFVNDLLLLKVGWAQGQWFVITVPFLGLIFFGVLLYKTVPLFNLE
HHHHHHHHHHCCCCCEECCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCC
DRPFVKEFAGTLQIRMTNSLLLFIMVLGLGIWGFYHLLQGEF
CCCHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA