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The map label for this gene is trmA-1 [C]

Identifier: 116326897

GI number: 116326897

Start: 64474

End: 65727

Strand: Reverse

Name: trmA-1 [C]

Synonym: LBL_0052

Alternate gene names: 116326897

Gene position: 65727-64474 (Counterclockwise)

Preceding gene: 116326900

Following gene: 116326896

Centisome position: 1.82

GC content: 37.24

Gene sequence:

>1254_bases
ATGAGATCCGAATCCGAGGGACATAAAAATCGTCCGAAAAAACGAATTCGCAAAATTTCTTCGGATAAAATTTTGCTGAA
ACCTCGGTCCTGGGTAAACTTAGGTTATACGATCGCAAATTCTGAGGAAGCGACTTTTTTTTTGAAAAATGCGATACCGG
GAGAAAAAGTTTATACGGTTCCTCTTAAAAAATCCGGCTCTTTAATTTGGGGAGTCGTCTCCGAAATCGAAGTGATTTCG
CCGGAAAGAATTCCATCCGATTGTAGTTCGTTTCCTCGTTGTGGAGGATGTTCTTATCGTCATATTTCTTATCAAAATGA
ATTGAAGATCAAAAAATTCCTTCTTCAAGAAACGCTCGAACGTTTTTTATCGAAAAATCATATTCAAATTCCTGAAATCG
AAATTCTCAGTGAAAATCCGAACGGGTATAGAAATACGGCACAGATTCAATTAGGATTTGTAGGTGGTAAAAGGCTTGCA
GGTTTTTATGAGGAATTTTCACATTCGATCGTAAATCTTCCCGAATATGGATGCAAAAATCTTCCGGAAGAAATGAATTC
GGTATTCTCTGATCTTTTGAAAAAAGAAAAAACAGAATCTATGTTCGCCTCAGGATCAAAAACTATTTCCTTGCGATTAG
AAGGAACGAAAGTTGTTCCCTACAAAAAAGAGTCGGTTACGTTTCGGGAAAAAATTTCCGTTCCGGAACTCAAGGAAATC
GTTTGGGAAATTCAAGCCGGAGGGTTTTCACAAATAAATCGATATCTTGTCGCCTCTTGGCTCGAAAAGATTTTCGAACT
GGTTCCGGAAAATCAAGATCGAATATTAGAATTGTATTGTGGTTCCGGTTTGATTGCAATTGCTCTTCAATCTAAAACCA
AAAAATGGATCGGTTACGAATTTTCTTTAAATTCCGTAAAACAGGCGAAAAAAAACGCTCAACGAAACGGGTTTTATTCC
TGCGATTTCAAAACTTTAAACTTGGAAACAAATTGGATCGATTCCGAAGAGGCATTGTATTCTTCTTTTTGGATCATGAA
CCCTCCAAGAGCGGGTTTATCTAAAAAAGTTCTACAAACGCTGATAGAAAAAAATCCACGCGGATTTTTATACTCAAGTT
GTAATCACACAACTCTTGCGAGAGATCTCTCTTTGATCTTGAACGGCAATTATAGACTTTCGAACGTGACGCTGGTCGAT
TTTTTTCCAAGAACCAAACATTTCGAAGTCATCGTAAAAGTTGAAAGAATTTAG

Upstream 100 bases:

>100_bases
ACTTCTCCTGACGGAATTACTTTACCGTGAGAAAACCCTCTCGTGTCAAGGTGAATTGTTGTTTGCGCTTGGGGATTCCT
GAAAACAACTACTAAAAGAG

Downstream 100 bases:

>100_bases
ACAAAATGGCGTCTCTTCCGAGTTTGAAAATTGTGGGTTAGATACAAAGCATTTCTCACAAAACAAGAAAACCAATTTTA
ATCTTTTTATTATCACTCCC

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MRSESEGHKNRPKKRIRKISSDKILLKPRSWVNLGYTIANSEEATFFLKNAIPGEKVYTVPLKKSGSLIWGVVSEIEVIS
PERIPSDCSSFPRCGGCSYRHISYQNELKIKKFLLQETLERFLSKNHIQIPEIEILSENPNGYRNTAQIQLGFVGGKRLA
GFYEEFSHSIVNLPEYGCKNLPEEMNSVFSDLLKKEKTESMFASGSKTISLRLEGTKVVPYKKESVTFREKISVPELKEI
VWEIQAGGFSQINRYLVASWLEKIFELVPENQDRILELYCGSGLIAIALQSKTKKWIGYEFSLNSVKQAKKNAQRNGFYS
CDFKTLNLETNWIDSEEALYSSFWIMNPPRAGLSKKVLQTLIEKNPRGFLYSSCNHTTLARDLSLILNGNYRLSNVTLVD
FFPRTKHFEVIVKVERI

Sequences:

>Translated_417_residues
MRSESEGHKNRPKKRIRKISSDKILLKPRSWVNLGYTIANSEEATFFLKNAIPGEKVYTVPLKKSGSLIWGVVSEIEVIS
PERIPSDCSSFPRCGGCSYRHISYQNELKIKKFLLQETLERFLSKNHIQIPEIEILSENPNGYRNTAQIQLGFVGGKRLA
GFYEEFSHSIVNLPEYGCKNLPEEMNSVFSDLLKKEKTESMFASGSKTISLRLEGTKVVPYKKESVTFREKISVPELKEI
VWEIQAGGFSQINRYLVASWLEKIFELVPENQDRILELYCGSGLIAIALQSKTKKWIGYEFSLNSVKQAKKNAQRNGFYS
CDFKTLNLETNWIDSEEALYSSFWIMNPPRAGLSKKVLQTLIEKNPRGFLYSSCNHTTLARDLSLILNGNYRLSNVTLVD
FFPRTKHFEVIVKVERI
>Mature_417_residues
MRSESEGHKNRPKKRIRKISSDKILLKPRSWVNLGYTIANSEEATFFLKNAIPGEKVYTVPLKKSGSLIWGVVSEIEVIS
PERIPSDCSSFPRCGGCSYRHISYQNELKIKKFLLQETLERFLSKNHIQIPEIEILSENPNGYRNTAQIQLGFVGGKRLA
GFYEEFSHSIVNLPEYGCKNLPEEMNSVFSDLLKKEKTESMFASGSKTISLRLEGTKVVPYKKESVTFREKISVPELKEI
VWEIQAGGFSQINRYLVASWLEKIFELVPENQDRILELYCGSGLIAIALQSKTKKWIGYEFSLNSVKQAKKNAQRNGFYS
CDFKTLNLETNWIDSEEALYSSFWIMNPPRAGLSKKVLQTLIEKNPRGFLYSSCNHTTLARDLSLILNGNYRLSNVTLVD
FFPRTKHFEVIVKVERI

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=258, Percent_Identity=28.2945736434109, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI51173878, Length=258, Percent_Identity=28.2945736434109, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI269784680, Length=215, Percent_Identity=28.3720930232558, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI269784676, Length=215, Percent_Identity=28.3720930232558, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI20149688, Length=215, Percent_Identity=28.3720930232558, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI269784678, Length=215, Percent_Identity=28.3720930232558, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1789148, Length=432, Percent_Identity=25.462962962963, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1787083, Length=135, Percent_Identity=28.8888888888889, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24666579, Length=280, Percent_Identity=27.5, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 47781; Mature: 47781

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSESEGHKNRPKKRIRKISSDKILLKPRSWVNLGYTIANSEEATFFLKNAIPGEKVYTV
CCCCCCCCCCCHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCEEEEECCCCCCEEEEE
PLKKSGSLIWGVVSEIEVISPERIPSDCSSFPRCGGCSYRHISYQNELKIKKFLLQETLE
ECCCCCCEEEEEHHHHEECCCCCCCHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
RFLSKNHIQIPEIEILSENPNGYRNTAQIQLGFVGGKRLAGFYEEFSHSIVNLPEYGCKN
HHHCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCC
LPEEMNSVFSDLLKKEKTESMFASGSKTISLRLEGTKVVPYKKESVTFREKISVPELKEI
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEECCCCCCCCCHHHHCCCCHHHHH
VWEIQAGGFSQINRYLVASWLEKIFELVPENQDRILELYCGSGLIAIALQSKTKKWIGYE
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCCHHEEEE
FSLNSVKQAKKNAQRNGFYSCDFKTLNLETNWIDSEEALYSSFWIMNPPRAGLSKKVLQT
EEHHHHHHHHHHHHHCCEEEECEEEEEEEECCCCCHHHHHHEEEEECCCCCCHHHHHHHH
LIEKNPRGFLYSSCNHTTLARDLSLILNGNYRLSNVTLVDFFPRTKHFEVIVKVERI
HHHCCCCCEEEECCCCCHHHHCEEEEEECCEEEEEEEEEEECCCCCCEEEEEEEECC
>Mature Secondary Structure
MRSESEGHKNRPKKRIRKISSDKILLKPRSWVNLGYTIANSEEATFFLKNAIPGEKVYTV
CCCCCCCCCCCHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCEEEEECCCCCCEEEEE
PLKKSGSLIWGVVSEIEVISPERIPSDCSSFPRCGGCSYRHISYQNELKIKKFLLQETLE
ECCCCCCEEEEEHHHHEECCCCCCCHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
RFLSKNHIQIPEIEILSENPNGYRNTAQIQLGFVGGKRLAGFYEEFSHSIVNLPEYGCKN
HHHCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCC
LPEEMNSVFSDLLKKEKTESMFASGSKTISLRLEGTKVVPYKKESVTFREKISVPELKEI
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEECCCCCCCCCHHHHCCCCHHHHH
VWEIQAGGFSQINRYLVASWLEKIFELVPENQDRILELYCGSGLIAIALQSKTKKWIGYE
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCCCHHEEEE
FSLNSVKQAKKNAQRNGFYSCDFKTLNLETNWIDSEEALYSSFWIMNPPRAGLSKKVLQT
EEHHHHHHHHHHHHHCCEEEECEEEEEEEECCCCCHHHHHHEEEEECCCCCCHHHHHHHH
LIEKNPRGFLYSSCNHTTLARDLSLILNGNYRLSNVTLVDFFPRTKHFEVIVKVERI
HHHCCCCCEEEECCCCCHHHHCEEEEEECCEEEEEEEEEEECCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA