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The map label for this gene is topB [H]

Identifier: 115353236

GI number: 115353236

Start: 3520118

End: 3522715

Strand: Direct

Name: topB [H]

Synonym: Bamb_3185

Alternate gene names: 115353236

Gene position: 3520118-3522715 (Clockwise)

Preceding gene: 115353235

Following gene: 115353238

Centisome position: 98.98

GC content: 63.74

Gene sequence:

>2598_bases
ATGTCCAAAGCTCTGATCATTGCGGAAAAGCCTTCTGTCGCGAACGACATCGCGCGCGCTTTGGGCGGCTTTACCAAGCA
TGACGAATACTTCGAAAGCGACGAATTCGTCCTTTCGTCCGCGGTCGGCCACCTGCTGGAAATCGCCGCCCCGGAAGAGT
ACGAGGTCAAGCGCGGCAAATGGAGCTTCGCTCATCTGCCCGTCATCCCCCCGCATTTCGACCTCAACCCGATTGCAAAA
AGCGAATCGCGCCTGAAGGTCCTTACCAAGCTGATGAAGCGCAAGGACGTCGACCGCCTGATCAACGCATGTGACGCGGG
GCGCGAGGGCGAACTGATTTTCCGCCTGATCGTGCAGCATGCGAAGGCGAAGCAGCCGGTCCAGCGCCTGTGGCTGCAGT
CGATGACGCCGCAGGCGATTCGCGACGGTTTCGCGCACCTGCGCAGCGACACCGACATGCAACCGCTCGCCGACGCCGCA
CGCTGCCGCTCCGAAGCCGACTGGCTCGTCGGGATCAACGGCACCCGCGCGATGACCGCGTTCAACAGCAAGGGCGGCGG
CTTCTTCCTGACGACCGTCGGCCGCGTTCAGACGCCAACGCTGTCGATCGTCGTCGAACGTGAAGAGAAAATCCGCCGCT
TCGTCCCGCGGGACTACTGGGAAGTGAAGGCCGCGTTCGCCTGCGCGAACGGCTTCTACGAAGGCAAGTGGTACGACCCG
AAATTCAAGCGGGACGAGTTCGATCCTGAAAGGCGCGACTCCCGCCTGTGGAGCCTGCCGGCCGCCGAAACCATCGTCGC
CGCATGCCGCGACCAGGTCGGCACGGTATCCGAGGAATCGAAGCCGTCGACCCAGCTGTCGCCGCTGCTGTTCGATCTGA
CGAGCCTGCAGCGCGAGGCGAACAGCCGCTTCGGCTTCTCCGCGAAGAATACGCTCGGCCTCGCGCAGGCGCTGTATGAA
AAGCACAAGGTGCTGACGTACCCGCGTACCGACGCGCGCGCGCTGCCGGAAGACTACATTTCGACGGTGCAGTCGACGCT
CGAAATGCTCAAGGAGAGCCACAACTACCTGCCGCATGCGAAGCGGGTGCTGGACCAGGGCTGGGTGAAGCCGAACAAGC
GGATCTTCGACAATTCGAAGATCAGCGACCACTTCGCCATCATCCCGACGCTGCAGGCGCCGAAGTCGCTGTCCGAGCCG
GAGCAGAAGCTGTACGACATGGTCGTGAAGCGCTTCCTCGCGGTGTTCTTCCCGGCGGCCGAATTCCGTGTCACGACGCG
GATCACCGAAGTCGCCGGTCATCACTTCAAGACCGAAGGCAAGGTGCTCGTCGAGCCGGGCTGGCTGCAGGTGTACGGCC
GCGACGCCGAAGGCGCGGACGCGAACCTCGTGCCGGTGCAGAAGGACGAGAAGGTGAAGACGGACGAGATCGCCGCCGTG
GCGCTCGTGACCAAGCCGCCCGCACGCTACTCCGAAGCGACGCTGTTGTCGGCAATGGAAGGCGCGGGCAAGCTCGTCGA
GGACGACGAGCTGCGCGAGGCGATGGCCGCGAAGGGCCTCGGCACGCCGGCAACGCGCGCGGCGATCATCGAAGGCCTAC
TGGGCGAAAAATATCTCGTCCGTGAAGGCCGCGAGCTGATCCCGACCGCGAAGGCATTCCAGCTGATGACGCTGCTGCGC
GGGCTCGGCGTGAAGGAGCTGACCGCGCCCGAGCTCACCGGCGAATGGGAATACAAGCTGTCGCAGATGGAGCGCGGCAA
CCTCGGCCGCGATGCGTTCATGCAGGAAATCGCCCGCATGACGCAGCAGATCGTCAAGCGCGCGAAGGAATACGATTCCG
ACACGATCCCCGGCGATTACGCGACGCTCCAGACGCCGTGCCCGAACTGCGGCGGCCAGGTGAAGGAAAACTACCGCCGC
TTCGCGTGCACGAAGTGCGAGTTCTCGATCTCGAAGATTCCGGGCAGCCGGCAGTTCGAGATCGCGGAAGTCGAGGAACT
GCTGCAGCAGAAGACCATCGGTCCGCTGTCCGGTTTCCGCAGCAAGATGGGCCGTCCGTTCTCGGCGATCCTGAAGCTCT
CGTTCGACGACGAAACGAAGAACTACAAGCTCGAGTTCGACTTCGGCCAGGACCAGGGCGGCGAGGAAGGCGAGGCGCCC
GACTTCTCCGAGCAGGAACCGGTCGGCGCGTGCCCGAAATGCAACGGCCGCGTGTTCGAGCACGGGATGAGCTATGTGTG
CGAGCACTCGGTCGCGAATCCGAAGACCTGCGACTTCCGTTCGGGCAAGGTGATCCTGCAGCAGGAAATCACGCGCGACC
AGATGGCCAAGCTGCTCGCCGACGGTCGCACGGATCTGATGCCGAACTTCAAGTCGTCGCGCACGGGCCGCAACTTCAAG
GCGTTCCTCGTGAAGCAGCCGGACGGCAAGATCGGTTTCGAGTTCGAAAAGAAGGAGCCGAAGGCTGCCGCCGCGAAGAA
GACGGCAAAATCGGCGACGAAGGATGCCGAAACCGTGACGGAAGGCGCCGACGAGAAACCGGCACCGGCCCGCAAGACCG
CCGCCCGCAAGACGACGGCGCGCAAGACGGGCTCGTAA

Upstream 100 bases:

>100_bases
GCTTTTTACACGCCGCGCCGCCGTCGCCGCCGCGTGTGCTATAAAGCCGTCGTAAAAGGGACCCACGCCGGCGAACCCGG
TCTACCAACACACACCTGTC

Downstream 100 bases:

>100_bases
CGACCCCACCGCCGGGGGGCTCCCCCCGGCGCATTGCACGCCGGTCAGCCGGTCAAACGGCGATAAAAAAACGCGGATCG
ATCGCTCGATCCGCGTTTTT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 865; Mature: 864

Protein sequence:

>865_residues
MSKALIIAEKPSVANDIARALGGFTKHDEYFESDEFVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAK
SESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIVQHAKAKQPVQRLWLQSMTPQAIRDGFAHLRSDTDMQPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAAFACANGFYEGKWYDP
KFKRDEFDPERRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREANSRFGFSAKNTLGLAQALYE
KHKVLTYPRTDARALPEDYISTVQSTLEMLKESHNYLPHAKRVLDQGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEP
EQKLYDMVVKRFLAVFFPAAEFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLVREGRELIPTAKAFQLMTLLR
GLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLQTPCPNCGGQVKENYRR
FACTKCEFSISKIPGSRQFEIAEVEELLQQKTIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDQGGEEGEAP
DFSEQEPVGACPKCNGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITRDQMAKLLADGRTDLMPNFKSSRTGRNFK
AFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVTEGADEKPAPARKTAARKTTARKTGS

Sequences:

>Translated_865_residues
MSKALIIAEKPSVANDIARALGGFTKHDEYFESDEFVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAK
SESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIVQHAKAKQPVQRLWLQSMTPQAIRDGFAHLRSDTDMQPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAAFACANGFYEGKWYDP
KFKRDEFDPERRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREANSRFGFSAKNTLGLAQALYE
KHKVLTYPRTDARALPEDYISTVQSTLEMLKESHNYLPHAKRVLDQGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEP
EQKLYDMVVKRFLAVFFPAAEFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLVREGRELIPTAKAFQLMTLLR
GLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLQTPCPNCGGQVKENYRR
FACTKCEFSISKIPGSRQFEIAEVEELLQQKTIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDQGGEEGEAP
DFSEQEPVGACPKCNGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITRDQMAKLLADGRTDLMPNFKSSRTGRNFK
AFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVTEGADEKPAPARKTAARKTTARKTGS
>Mature_864_residues
SKALIIAEKPSVANDIARALGGFTKHDEYFESDEFVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAKS
ESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIVQHAKAKQPVQRLWLQSMTPQAIRDGFAHLRSDTDMQPLADAAR
CRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAAFACANGFYEGKWYDPK
FKRDEFDPERRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREANSRFGFSAKNTLGLAQALYEK
HKVLTYPRTDARALPEDYISTVQSTLEMLKESHNYLPHAKRVLDQGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEPE
QKLYDMVVKRFLAVFFPAAEFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAVA
LVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLVREGRELIPTAKAFQLMTLLRG
LGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLQTPCPNCGGQVKENYRRF
ACTKCEFSISKIPGSRQFEIAEVEELLQQKTIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDQGGEEGEAPD
FSEQEPVGACPKCNGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITRDQMAKLLADGRTDLMPNFKSSRTGRNFKA
FLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVTEGADEKPAPARKTAARKTTARKTGS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=649, Percent_Identity=26.040061633282, Blast_Score=145, Evalue=2e-34,
Organism=Homo sapiens, GI4507635, Length=655, Percent_Identity=24.2748091603053, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI1788061, Length=620, Percent_Identity=34.5161290322581, Blast_Score=332, Evalue=6e-92,
Organism=Escherichia coli, GI1787529, Length=669, Percent_Identity=25.7100149476831, Blast_Score=155, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32563869, Length=626, Percent_Identity=25.2396166134185, Blast_Score=132, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=634, Percent_Identity=23.8170347003155, Blast_Score=98, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323263, Length=668, Percent_Identity=25, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585251, Length=651, Percent_Identity=25.652841781874, Blast_Score=122, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640096, Length=640, Percent_Identity=22.03125, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 96607; Mature: 96476

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKALIIAEKPSVANDIARALGGFTKHDEYFESDEFVLSSAVGHLLEIAAPEEYEVKRGK
CCCEEEEECCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCHHCCCC
WSFAHLPVIPPHFDLNPIAKSESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIVQH
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AKAKQPVQRLWLQSMTPQAIRDGFAHLRSDTDMQPLADAARCRSEADWLVGINGTRAMTA
HHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAAFACANGFYEGKWYDP
ECCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
KFKRDEFDPERRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREA
CCCCCCCCHHHHCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
NSRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYISTVQSTLEMLKESHNYLPHA
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCHHH
KRVLDQGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEPEQKLYDMVVKRFLAVFFPAA
HHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
EFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
HHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCCEECCCCCCCCCHHHEEEE
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLV
EEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHH
REGRELIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARM
HCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
TQQIVKRAKEYDSDTIPGDYATLQTPCPNCGGQVKENYRRFACTKCEFSISKIPGSRQFE
HHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCEEHHHCCCCCCEE
IAEVEELLQQKTIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDQGGEEGEAP
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DFSEQEPVGACPKCNGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITRDQMAKLLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DGRTDLMPNFKSSRTGRNFKAFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVT
CCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHH
EGADEKPAPARKTAARKTTARKTGS
CCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKALIIAEKPSVANDIARALGGFTKHDEYFESDEFVLSSAVGHLLEIAAPEEYEVKRGK
CCEEEEECCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCHHCCCC
WSFAHLPVIPPHFDLNPIAKSESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIVQH
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AKAKQPVQRLWLQSMTPQAIRDGFAHLRSDTDMQPLADAARCRSEADWLVGINGTRAMTA
HHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFVPRDYWEVKAAFACANGFYEGKWYDP
ECCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
KFKRDEFDPERRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREA
CCCCCCCCHHHHCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
NSRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYISTVQSTLEMLKESHNYLPHA
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCHHH
KRVLDQGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEPEQKLYDMVVKRFLAVFFPAA
HHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
EFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
HHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCCEECCCCCCCCCHHHEEEE
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLV
EEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHH
REGRELIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARM
HCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
TQQIVKRAKEYDSDTIPGDYATLQTPCPNCGGQVKENYRRFACTKCEFSISKIPGSRQFE
HHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCEEHHHCCCCCCEE
IAEVEELLQQKTIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDQGGEEGEAP
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DFSEQEPVGACPKCNGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITRDQMAKLLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DGRTDLMPNFKSSRTGRNFKAFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVT
CCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHH
EGADEKPAPARKTAARKTTARKTGS
CCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA