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The map label for this gene is purR [H]

Identifier: 115353070

GI number: 115353070

Start: 3339242

End: 3340261

Strand: Direct

Name: purR [H]

Synonym: Bamb_3019

Alternate gene names: 115353070

Gene position: 3339242-3340261 (Clockwise)

Preceding gene: 115353069

Following gene: 115353071

Centisome position: 93.89

GC content: 72.75

Gene sequence:

>1020_bases
ATGGGCACCACCATTCGCGACGTAGCGCGGGCGGCAGAGGTCTCGATCGGCACCGTCTCCCGCGCGCTGAAAAACCAGCC
GGGCCTGTCCGAAGCCACGCGCGCGCGCATCGTCGAGATCGCGCAGCGGCTCGGTTACGACCCGGCCCAGTTGCGCCCGC
GCATCCGCCGGCTCACCTTCCTGCTGCACCGCCAGCACAACCGCTTTCCCGCCAGTCCGTTCTTCTCGCACGTGCTGCAC
GGCGTCGAGGACGCATGCCGCGAGCGCGGCATCGTGCCGACGCTGCTCACGGTCGGCCCGAACGACGACGTGCTGCGCCA
GATGCGCCCGCACGCGCCGGACGCGATCGCGGTGGCCGGCTTCATCGAGCCCGAGACGATCGAGGCGCTCGCCGCGACCG
GCCGCCCGCTCGTGCTGATCGACCTGTGGGCGCCCGGCCTGCGCTCGGTGAACATCGACAACGCGACGGGCGCGGCGCTC
GCGATGCGCCACCTGCTCGCCACCGGCCGCTCGCGGATCGCGTTCATCGGCGGCTCCGCCGCGCACTACAGCATCGCGCA
GCGCGCGATCGGCTATCGCCGCGCGTTCTTCGAGGCCGGGCGGCTGTTCGATCCCGCATACGAAGTGACGATCGACGCCG
GGCTCGACCCCGACACCGGCGCCACGCGCGCGATGCAGCAGCTGCTCGACGCGCCGGGCCCGCGCCCCGACGCCGTGTTC
GCGTACAACGACGCCGCCGCGCTGGCCGCGCAGCGCGTGTGCATCGCGCGCGGCGTGCGGATTCCGGAAGACATCGCGAT
CGTCGGCTTCGACGACATCCCCGCCGCCGCGCACGCGAACCCGCCGCTCACGACGCTCGCCGTCGACAAGGAAGCGCTCG
GCCGCCGCGGCGTCGAACTGCTGCTCGCCGAAGCGCCCGAGCGCACCGAGATCTCCCTGCCCGTCGAGCTGATCGTGCGG
GCCAGCAGCCAGCCCGCCGGCTCACCGGCACTCGATACCGCCACGGTCACCGAATCATGA

Upstream 100 bases:

>100_bases
AACCCGTTATTTCCGGGTATTCCCCGGTTATCCGGCCCGAATTTGGCGCCGAGTTTTACTATACAATCGCCGTCACCCTA
AACAAACCGGATCCCGAACG

Downstream 100 bases:

>100_bases
ACATGCCCCCGGTCCAATCCCGCACGGCCACGCCGGCCAACCACGCGCAAGCGGCGCCGTTCGTCGCGAGCTTCCGCGAT
CCGTCGTTCCTGCTGTCGCA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MGTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVEIAQRLGYDPAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLH
GVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAAL
AMRHLLATGRSRIAFIGGSAAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGATRAMQQLLDAPGPRPDAVF
AYNDAAALAAQRVCIARGVRIPEDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVELLLAEAPERTEISLPVELIVR
ASSQPAGSPALDTATVTES

Sequences:

>Translated_339_residues
MGTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVEIAQRLGYDPAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLH
GVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAAL
AMRHLLATGRSRIAFIGGSAAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGATRAMQQLLDAPGPRPDAVF
AYNDAAALAAQRVCIARGVRIPEDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVELLLAEAPERTEISLPVELIVR
ASSQPAGSPALDTATVTES
>Mature_338_residues
GTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVEIAQRLGYDPAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLHG
VEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALA
MRHLLATGRSRIAFIGGSAAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGATRAMQQLLDAPGPRPDAVFA
YNDAAALAAQRVCIARGVRIPEDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVELLLAEAPERTEISLPVELIVRA
SSQPAGSPALDTATVTES

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=351, Percent_Identity=32.1937321937322, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=32.5878594249201, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789456, Length=320, Percent_Identity=30, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787948, Length=328, Percent_Identity=28.6585365853659, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=30.6122448979592, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=29.8780487804878, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI48994940, Length=338, Percent_Identity=28.4023668639053, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.3492063492064, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789068, Length=298, Percent_Identity=26.510067114094, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786540, Length=353, Percent_Identity=26.0623229461756, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787906, Length=269, Percent_Identity=28.2527881040892, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36277; Mature: 36146

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVEIAQRLGYDPAQLRPRIRRLTF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLHRQHNRFPASPFFSHVLHGVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCCCEEEEE
FIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALAMRHLLATGRSRIAFIGGSA
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEECCCH
AHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGATRAMQQLLDAPGPRPDAVF
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEE
AYNDAAALAAQRVCIARGVRIPEDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVEL
EECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECHHHHHCCCCEE
LLAEAPERTEISLPVELIVRASSQPAGSPALDTATVTES
EEECCCCCCEECCCHHHEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVEIAQRLGYDPAQLRPRIRRLTF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLHRQHNRFPASPFFSHVLHGVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCCCEEEEE
FIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALAMRHLLATGRSRIAFIGGSA
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEECCCH
AHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGATRAMQQLLDAPGPRPDAVF
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEE
AYNDAAALAAQRVCIARGVRIPEDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVEL
EECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECHHHHHCCCCEE
LLAEAPERTEISLPVELIVRASSQPAGSPALDTATVTES
EEECCCCCCEECCCHHHEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA