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The map label for this gene is bioB [H]

Identifier: 115353034

GI number: 115353034

Start: 3292258

End: 3293277

Strand: Direct

Name: bioB [H]

Synonym: Bamb_2983

Alternate gene names: 115353034

Gene position: 3292258-3293277 (Clockwise)

Preceding gene: 115353033

Following gene: 115353037

Centisome position: 92.57

GC content: 67.55

Gene sequence:

>1020_bases
ATGACCCAAGCCCAGACCGCCACCGTGCAACCCGACGCGATTCCTGTCGCCGCTCCGGCCTCGCAGCGCTGGCGCGTCGC
CGACGTCGTCGCGCTGTTCGAGCTGCCGTTCAACGACCTGATCTTCCGCGCGCAGCAGGTGCATCGCGAACACTTCGACG
CGAACGCGGTGCAGCTGTCGACGCTGCTGTCGATCAAGACGGGCGGCTGCGAGGAAGATTGCGGCTACTGCTCGCAGTCG
TCGCACCACGACACGGGCCTGAAGGCCGAGAAGCTGATGGACGTCGACGCGGTGCTCGACGCCGCGCGCGCGGCGAAGGC
GAACGGCGCGAGCCGCTTCTGCATGGGCGCCGCATGGCGCAACCCGAAGGAGCGCCACATGCCAGCGCTGACCGAGATGG
TGCGTGGCGTGAAGGAACTCGGCCTCGAAACCTGCATGACGCTCGGCATGCTGGAAGACGAACAGGCGCAGGAACTCGCG
AACGCGGGCCTCGACTACTACAACCACAACCTCGACACGTCGCCGGAGTTCTACGGCCAGGTGATCTCGACGCGCACGTA
CCAGGATCGCCTCGACACGCTCGACCGCGTGCGCGACGCGGGCATCAACGTGTGCTGCGGCGGCATCATCGGGATGGGTG
AATCGCGCCGCGAACGCGCGGGCCTGATCTCGCAGCTCGCGAACCTGAACCCGTATCCGGATTCGGTGCCGATCAACAAC
CTCGTCGCGATCGAAGGCACGCCGCTCGAAGGCACCGCACCGCTCGACCCGTTCGAATTCGTGCGCACGATCGCGGTCGC
GCGCATCACGATGCCGAAGGCCGTCGTGCGCCTGTCGGCCGGCCGCGAGCAGCTCGACGACGGGCTGCAGGCGATGTGCT
TCCTCGCCGGCGCGAACTCGATGTTCTACGGCGACCAGCTGCTGACGACGAGCAACCCGCAGTCGCAGAAGGATCGCGCG
CTGTTCGAGCGCCTCGGCATCCGCGCGAGCGACGCGGACGCGATGCAGGCGAACGCGTAA

Upstream 100 bases:

>100_bases
GACATCGCCGCGCTCGTCGAGACGCTGCGCGCCGCGCAGCATTGACCCAACAGAAACAGATCCCCCGATCCCTCATCCCC
GTCCAGGACAGGAAAACGAT

Downstream 100 bases:

>100_bases
GCGGCAGCACCGCGTACCAACGAAAAAGCCGGGGCATGCCCCGGCTTTTTCTTGCCCGATCGTCCGCATCGGGTCGCGCT
GCGCGTCGCGAAGCGTGCGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTQAQTATVQPDAIPVAAPASQRWRVADVVALFELPFNDLIFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQS
SHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQELA
NAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPINN
LVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGANSMFYGDQLLTTSNPQSQKDRA
LFERLGIRASDADAMQANA

Sequences:

>Translated_339_residues
MTQAQTATVQPDAIPVAAPASQRWRVADVVALFELPFNDLIFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQS
SHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQELA
NAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPINN
LVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGANSMFYGDQLLTTSNPQSQKDRA
LFERLGIRASDADAMQANA
>Mature_338_residues
TQAQTATVQPDAIPVAAPASQRWRVADVVALFELPFNDLIFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQSS
HHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQELAN
AGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPINNL
VAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGANSMFYGDQLLTTSNPQSQKDRAL
FERLGIRASDADAMQANA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=65.9016393442623, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321725, Length=320, Percent_Identity=48.125, Blast_Score=324, Evalue=9e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36954; Mature: 36823

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAQTATVQPDAIPVAAPASQRWRVADVVALFELPFNDLIFRAQQVHREHFDANAVQLS
CCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
TLLSIKTGGCEEDCGYCSQSSHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWR
HHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
NPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQELANAGLDYYNHNLDTSPEFYGQ
CCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCCCCCHHHHHH
VISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPINN
HHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
LVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGANS
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
MFYGDQLLTTSNPQSQKDRALFERLGIRASDADAMQANA
CEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
TQAQTATVQPDAIPVAAPASQRWRVADVVALFELPFNDLIFRAQQVHREHFDANAVQLS
CCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
TLLSIKTGGCEEDCGYCSQSSHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWR
HHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
NPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQELANAGLDYYNHNLDTSPEFYGQ
CCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHCCCCCCCHHHHHH
VISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPINN
HHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
LVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGANS
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
MFYGDQLLTTSNPQSQKDRALFERLGIRASDADAMQANA
CEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA