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The map label for this gene is bioD

Identifier: 115353033

GI number: 115353033

Start: 3291483

End: 3292202

Strand: Direct

Name: bioD

Synonym: Bamb_2982

Alternate gene names: 115353033

Gene position: 3291483-3292202 (Clockwise)

Preceding gene: 115353032

Following gene: 115353034

Centisome position: 92.55

GC content: 72.5

Gene sequence:

>720_bases
ATGACCGCCCCGCTCTCGCTGTTCGTCACCGGCACCGACACCGAAATCGGCAAGACCTTCGTCTCGGCTGCGATGCTGCA
CGGCTTCGCGCGGCATGGCCTGCGTGCAGCCGCGCTGAAGCCCGTCGCGGCGGGCGCGTATGAACGCGACGGTGTCTGGC
GCAACGAGGACGCCGACCAGCTCGACGCGGCCGCCAACGTCGTGCTGCCGCCCGAGCTGCGCACGCCGTTCCTGCTGAAG
GCGCCCGCCGCGCCGCACATCGTCGCCGCGCAGGAAGGCGTGACGCTCGACATCGACACGATCGTCGCGTGCCATCGCGA
AGCGCTGACGCGCGCGGACGTCGTCGTCGTCGAGGGCGTCGGCGGCTTCCGCGTGCCGCTCACCGACACGCAGGACACGG
CCGATCTCGCGGTCGCGCTCGGCCTACCGGTGGTGCTCGTCGTCGGCGTGCGGCTCGGCTGCATCAGCCATGCGCTGCTC
ACCGCCGACGCGATTCGCCAGCGCGGCCTCACGCTCGCCGGCTGGGTCGCGAACCACGTCGACCCGGCGATGTCGTACGC
CGACGAGAACGTCGCGACGATTCGCGACTGGCTCGCCCGCGAGCACGGCGCGCCGCTGCTCGGCCGCATTCCGCACATGA
GCCCGGCCGCCGCCGAATCCGCCGCGGCGATGCTCGACATCGCCGCGCTCGTCGAGACGCTGCGCGCCGCGCAGCATTGA

Upstream 100 bases:

>100_bases
CGGCCCGGCGGGATGCAGAACAACCCCCACGCTCACTGCGTTCGCTGCCCCCCGAGGGGGCTGCAGCCTCCCTTGGGGCG
GCCCGGCGGGAGACTGCGGC

Downstream 100 bases:

>100_bases
CCCAACAGAAACAGATCCCCCGATCCCTCATCCCCGTCCAGGACAGGAAAACGATATGACCCAAGCCCAGACCGCCACCG
TGCAACCCGACGCGATTCCT

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTAPLSLFVTGTDTEIGKTFVSAAMLHGFARHGLRAAALKPVAAGAYERDGVWRNEDADQLDAAANVVLPPELRTPFLLK
APAAPHIVAAQEGVTLDIDTIVACHREALTRADVVVVEGVGGFRVPLTDTQDTADLAVALGLPVVLVVGVRLGCISHALL
TADAIRQRGLTLAGWVANHVDPAMSYADENVATIRDWLAREHGAPLLGRIPHMSPAAAESAAAMLDIAALVETLRAAQH

Sequences:

>Translated_239_residues
MTAPLSLFVTGTDTEIGKTFVSAAMLHGFARHGLRAAALKPVAAGAYERDGVWRNEDADQLDAAANVVLPPELRTPFLLK
APAAPHIVAAQEGVTLDIDTIVACHREALTRADVVVVEGVGGFRVPLTDTQDTADLAVALGLPVVLVVGVRLGCISHALL
TADAIRQRGLTLAGWVANHVDPAMSYADENVATIRDWLAREHGAPLLGRIPHMSPAAAESAAAMLDIAALVETLRAAQH
>Mature_238_residues
TAPLSLFVTGTDTEIGKTFVSAAMLHGFARHGLRAAALKPVAAGAYERDGVWRNEDADQLDAAANVVLPPELRTPFLLKA
PAAPHIVAAQEGVTLDIDTIVACHREALTRADVVVVEGVGGFRVPLTDTQDTADLAVALGLPVVLVVGVRLGCISHALLT
ADAIRQRGLTLAGWVANHVDPAMSYADENVATIRDWLAREHGAPLLGRIPHMSPAAAESAAAMLDIAALVETLRAAQH

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=207, Percent_Identity=45.4106280193237, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI87081944, Length=228, Percent_Identity=36.4035087719298, Blast_Score=136, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324385, Length=171, Percent_Identity=35.0877192982456, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 25058; Mature: 24927

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLSLFVTGTDTEIGKTFVSAAMLHGFARHGLRAAALKPVAAGAYERDGVWRNEDADQ
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCHHHCCCCCCCCCCCCCCHHH
LDAAANVVLPPELRTPFLLKAPAAPHIVAAQEGVTLDIDTIVACHREALTRADVVVVEGV
HHHHCCEEECCCCCCCEEEECCCCCCEEEECCCCEEEHHHHHHHHHHHHHHCCEEEEECC
GGFRVPLTDTQDTADLAVALGLPVVLVVGVRLGCISHALLTADAIRQRGLTLAGWVANHV
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHH
DPAMSYADENVATIRDWLAREHGAPLLGRIPHMSPAAAESAAAMLDIAALVETLRAAQH
CHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAPLSLFVTGTDTEIGKTFVSAAMLHGFARHGLRAAALKPVAAGAYERDGVWRNEDADQ
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHCHHHCCCCCCCCCCCCCCHHH
LDAAANVVLPPELRTPFLLKAPAAPHIVAAQEGVTLDIDTIVACHREALTRADVVVVEGV
HHHHCCEEECCCCCCCEEEECCCCCCEEEECCCCEEEHHHHHHHHHHHHHHCCEEEEECC
GGFRVPLTDTQDTADLAVALGLPVVLVVGVRLGCISHALLTADAIRQRGLTLAGWVANHV
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHH
DPAMSYADENVATIRDWLAREHGAPLLGRIPHMSPAAAESAAAMLDIAALVETLRAAQH
CHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA